1.School of Basic Medicine, Shanxi University of Chinese Medicine,Jinzhong 030600, China 2. People's Hospital of Zhenlai County,Jilin Province, Jilin Baicheng ,030024, China 3.Institute of Information, Traditional Chinese Medicine of China Academy of Chinese Medical Sciences,Beijing 100700,China 4.College of Pharmacy, Minzu University of China,Beijing 100081,China
Objective To explore the molecular mechanism of Gastrodia rhizoma in the prevention of headache, and to predict the potential target. Methods Using Integrated Pharmacological platform of traditional Chinese Medicine(TCM-IP) to predict the target based on Chemical Information of Gastrodia rhizoma and Construct the PPI (protein-protein interaction) network with target information for headache related diseases,enriching and analyzing the biological gene function and related pathways of Gastrodia rhizoma, diseases and key targets. constructing the interaction network with Chemical ingredients of Gastrodia rhizoma, key targets and drawing the “Gastrodia rhizoma -key Target-Pathway” Network. Results The active constituents of Gastrodia rhizoma Blume include ingphenols, polysaccharides and other ingredients, such as gastrodin, bis (4-hydroxyphenyl) ether beta-D-glucopyranoside, citricacid, xanthostrumarin/sitosterol 3-O-beta-D-pyran glucoside, hexadecanoic acid for the prevention of headache; 118 core target information was obtained for the prevention and treatment of headache in Gastrodia rhizoma, including 9 Putative Drug Target (potential drug target),such as GCK (Glucokinase),ATP1A1(Sodium/potassium transported alkaline phosphatase subunit α-1) and RPIA (5- phosphate isomerase A); 27 Known Disease Target , including HTR2B (serotonin receptor 2B), HTR1B (serotonin receptor 1B), HTR1D (serotonin receptor 1D), HTR1A (serotonin receptor 1A), PIK3CA (phosphatidylinositol 3-kinase) etc. Conclusion Based on Traditional Chinese medicine integrated pharmacology platform V1.0, this study analyzed the molecular mechanism of Gastrodia rhizoma in preventing and treating headache, and predicted the potential drug targets of Gastrodia rhizoma against headache, which will provide scientific basis for further experimental research of Gastrodia rhizoma.
周荣荣,李培硕,关伟,王丽萍,李彦文,李志勇. 基于整合药理学平台天麻治疗头痛的作用机制*[J]. 医药导报, 2019, 38(6): 706-710.
Rongrong ZHOU,Peishuo LI,Wei GUAN,Liping WANG,Yanwen LI,Zhiyong LI. Mechanism of Gastrodia Rhizoma in the Treatment of Headache Based on Integrated Pharmacology Platform. Herald of Medicine, 2019, 38(6): 706-710.
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