Objective To study the protective effect of grape seed oil (GSO) on methimazole (MMI) induced hypothyroidism mice, and to test its effect on the expression of serum inflammatory factors IL-1β,IL-2 and INF-γ. Methods A total of 24 female mice were randomly divided into three groups: normal control group, model control group and grape seed oil group (n=8). Animal model was constructed by gavage administration of MMI. Then the mice were sacrificed after 1 mg·kg-1·d-1GSO treatment for 4 weeks. The spleen, thymus and thyroid tissue were separated . The function of thyroid gland and the content of cytokines in mice were mesured. Results Body weight of normal control group was (23.54±1.06) g, model control group was (20.80±1.32) g and grape seed oil group was (20.28±0.82)g. GSO could reduce the spleen quality comparing with model control group(P<0.05). GSO treatment could increase the production of FT3 and FT4 (P<0.05) and significantly down-regulate the expression of IL-1β and IL-2 in serum. Conclusion GSO administration could provide protective effects on hypothyroidism through reducing inflammation and regulating the expression of IL-1β and IL-2.
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