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医药导报  2019, Vol. 38 Issue (6): 721-725    DOI: 10.3870/j.issn.1004-0781.2019.06.007
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重楼总皂苷对肝癌HepG2细胞放射敏感性的影响*
钟勇1(),但卫斌1(),谢俊杰2,刘江勇3,邵志雄2,易峰涛2,邓波4
1.湖北省通城县人民医院肿瘤科,咸宁 437400
2.中国人民解放军中部战区总医院放射治疗科,武汉 430070
3.中国人民解放军中部战区总医院放射科,武汉 430070
4.湖北省咸宁市中心医院肿瘤中心,咸宁 437000
Effects of Total Saponins from Rhizoma Paris on Radiosensitivity of Hepatocellular Carcinoma HepG2 Cells
Yong ZHONG1(),Weibin DAN1(),Junjie XIE2,Jiangyong LIU3,Zhixiong SHAO2,Fengtao YI2,Bo DENG4
1.Department of Oncology, the People's Hospital of Tongcheng County,Hubei Province, Xianning 437400,China
2.Department of Rradiotherapy,General Hospital of Central Theater Command of Chinese People’s Liberation Army, Wuhan 430070,China
3.Department of Radiology,General Hospital of Central Theater Command of Chinese People’s Liberation Army, Wuhan 430070,China
4.Cancer Center of Xianning Central Hospital, Hubei Province,Xianning 437000,China
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摘要 

目的 探讨重楼总皂苷(RPTS)对肝癌HepG2细胞放射敏感性的影响及其机制。方法 HepG2细胞培养后分为4组。空白对照组:用正常培养液继续培养48 h;RPTS组:用低细胞毒性浓度的RPTS(25 μg·mL-1)干预48 h;单纯照射组:先用正常培养液继续培养48 h,再用6 MV的X线进行照射;照射+RPTS组:先用低细胞毒性浓度的RPTS(25 μg·mL-1)干预48 h,再用6 MV的X线进行照射。采用CCK-8法检测重楼总皂苷对HepG2细胞增殖的影响;流式细胞术检测重楼总皂苷对细胞周期及凋亡的影响; Western blotting法检测MUC-1蛋白的表达情况。结果 与空白对照组比较,RPTS能明显抑制HepG2细胞增殖,其抑制率随药物浓度的增加、药物持续时间的延长明显增大。与RPTS组比较,照射+RPTS组早期凋亡率和晚期凋亡率明显增加(P<0.05)。照射+RPTS组S期细胞明显减少,MUC-1蛋白的表达最低,呈现时间依赖性(P<0.05)。结论 重楼总皂苷对HepG2细胞具有一定的放疗增敏作用,其机制可能与抑制细胞增殖、调节细胞周期、促进细胞凋亡、降低MUC-1蛋白的表达有关。

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钟勇
但卫斌
谢俊杰
刘江勇
邵志雄
易峰涛
邓波
关键词 重楼总皂苷肝癌增殖抑制放射敏感性MUC-1蛋白    
Abstract

Objective To investigate the effect of total saponins from Rhizoma Paris (RPTS) on radiosensitivity of hepatocellular carcinoma (HepG2) cells and its possible mechanism. Methods HepG2 cells were divided into 4 groups:blank control group, cultured with normal culture solution for 48 h; RPTS group, treated with RPTS (25 μg·mL-1) for 48 h; irradiation group, after culture with normal culture solution for 48 h, the cells were irradiated by 6 MV X ray; irradiation+RPTS group, after treatment with RPTS (25 μg·mL-1) for 48 h, the cells were irradiated by 6 MV X ray. CCK-8 was used to detect effect of total saponins on proliferation of HepG2. Influence of total saponins on cell cycle and apoptosis was detected by flow cytometry. The expression of MUC-1 protein was detected by Western blotting. Results The RPTS had inhibitory effects on HepG2 cells in a time and concentration dependent manner. The low cytotoxic concentration of RPTS regulated the cell cycle distribution of HepG2 cells.The low cytotoxic concentration of total saponins from Rhizoma Parisfurther induced apoptosis of hepatoma cells by X-ray(P<0.05).The low cytotoxic concentration of RPTS further inhibited the expression of MUC-1 protein (P<0.01). Conclusion RPTS have a certain radiosensitization effect on HepG2 cells, which may be related to the inhibition of cell proliferation, regulation of cell cycle, promotion of cell apoptosis and the reduction of the expression of MUC-1 protein.

Key wordsTotal saponin from Rhizoma Paris    Liver cancer    Proliferation inhibition    Radiosensibility    MUC-1 protein
收稿日期: 2018-04-03      出版日期: 2019-06-11
基金资助:*湖北省卫计委科研项目(2017H0054)
引用本文:   
钟勇,但卫斌,谢俊杰,刘江勇,邵志雄,易峰涛,邓波. 重楼总皂苷对肝癌HepG2细胞放射敏感性的影响*[J]. 医药导报, 2019, 38(6): 721-725.
Yong ZHONG,Weibin DAN,Junjie XIE,Jiangyong LIU,Zhixiong SHAO,Fengtao YI,Bo DENG. Effects of Total Saponins from Rhizoma Paris on Radiosensitivity of Hepatocellular Carcinoma HepG2 Cells. Herald of Medicine, 2019, 38(6): 721-725.
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http://www.yydbzz.com/CN/10.3870/j.issn.1004-0781.2019.06.007      或      http://www.yydbzz.com/CN/Y2019/V38/I6/721
图1  RPTS对HepG2细胞生长抑制曲线
组别 G0/G1 S期 G2/M期
空白对照组 77.19±2.49 11.09±0.55 11.72±1.95
RPTS组 49.8±6.55 35.07±2.47 15.12±5.04
单纯照射组 85.90±4.92 11.40±3.37 2.70±1.58
照射+RPTS组 88.74±1.07 5.62±0.44 5.64±1.05
表1  4组HepG2细胞作用48 h后细胞周期分布情况
组别 正常活细胞 凋亡早期细胞
空白对照组 99.72±0.26 0.10±0.14
RPTS组 92.43±0.55 4.07±0.34
单纯照射组 92.14±0.48 4.20±0.21
照射+RPTS组 83.50±0.60 11.68±0.12
组别 凋亡晚期细胞 死亡细胞
空白对照组 0.00±0.00 0.18±0.31
RPTS组 3.35±0.18 0.15±0.05
单纯照射组 3.51±0.25 0.16±0.07
照射+RPTS组 4.61±0.61 0.21±0.03
表2  4组HepG2细胞48 h后细胞凋亡变化情况
图2  4组HepG2细胞在48和72 h MUC-1蛋白表达
A.空白对照组(48 h);B.空白对照组(72 h);C.RPTS组(48 h);D.RPTS组(72 h);E.单纯照射组(48 h);F.单纯照射组(72 h);G.照射+RPTS组(48 h);H.照射+RPTS组(72 h)
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