1.Department of Oncology, the People's Hospital of Tongcheng County,Hubei Province, Xianning 437400,China 2.Department of Rradiotherapy,General Hospital of Central Theater Command of Chinese People’s Liberation Army, Wuhan 430070,China 3.Department of Radiology,General Hospital of Central Theater Command of Chinese People’s Liberation Army, Wuhan 430070,China 4.Cancer Center of Xianning Central Hospital, Hubei Province,Xianning 437000,China
Objective To investigate the effect of total saponins from Rhizoma Paris (RPTS) on radiosensitivity of hepatocellular carcinoma (HepG2) cells and its possible mechanism. Methods HepG2 cells were divided into 4 groups:blank control group, cultured with normal culture solution for 48 h; RPTS group, treated with RPTS (25 μg·mL-1) for 48 h; irradiation group, after culture with normal culture solution for 48 h, the cells were irradiated by 6 MV X ray; irradiation+RPTS group, after treatment with RPTS (25 μg·mL-1) for 48 h, the cells were irradiated by 6 MV X ray. CCK-8 was used to detect effect of total saponins on proliferation of HepG2. Influence of total saponins on cell cycle and apoptosis was detected by flow cytometry. The expression of MUC-1 protein was detected by Western blotting. Results The RPTS had inhibitory effects on HepG2 cells in a time and concentration dependent manner. The low cytotoxic concentration of RPTS regulated the cell cycle distribution of HepG2 cells.The low cytotoxic concentration of total saponins from Rhizoma Parisfurther induced apoptosis of hepatoma cells by X-ray(P<0.05).The low cytotoxic concentration of RPTS further inhibited the expression of MUC-1 protein (P<0.01). Conclusion RPTS have a certain radiosensitization effect on HepG2 cells, which may be related to the inhibition of cell proliferation, regulation of cell cycle, promotion of cell apoptosis and the reduction of the expression of MUC-1 protein.
钟勇,但卫斌,谢俊杰,刘江勇,邵志雄,易峰涛,邓波. 重楼总皂苷对肝癌HepG2细胞放射敏感性的影响*[J]. 医药导报, 2019, 38(6): 721-725.
Yong ZHONG,Weibin DAN,Junjie XIE,Jiangyong LIU,Zhixiong SHAO,Fengtao YI,Bo DENG. Effects of Total Saponins from Rhizoma Paris on Radiosensitivity of Hepatocellular Carcinoma HepG2 Cells. Herald of Medicine, 2019, 38(6): 721-725.
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