Objective To determine whether 7-methoxy-4’-hydroxyisoflavone promote maturation of osteoblasts. Methods Osteoblasts was treated by different concentrations (0, 10-4, 10-5, 10-6, 10-7, 10-8 mol·L-1) of 7-methoxy-4’-hydroxyisoflavone. The concentration of osteocalcin(OC) and alkaline phosphatase(ALP) were determined.The expression levels of protein sGC and PKG-1 in cells were detected by Western blotting. Results The group of 10-6 mol·L-1 significantly increased ALP activity and OC content in osteoblasts. The effect of L-NMA on ALP activity and OC content in osteoblasts was inhibited, and the expression of NOS, NO, cGMP, sGC and PKG was inhibited Conclusion 7-methoxy-4’-hydroxyisoflavone promoted osteoblasts differentiation through NO/cGMP/sGC signal pathway.
宋明甲,文益民,吴晓燕,柴晓亮,周建. 7-甲氧基-4’-羟基异黄酮促进大鼠成骨细胞成骨性分化的作用机制*[J]. 医药导报, 2019, 38(6): 730-734.
Mingjia SONG,Yiming WEN,Xiaoyan WU,Xiaoliang CHAI,Jian ZHOU. Mechanism of 7-Methoxy-4’-hydroxyisoflavone on the Promotion of OsteogenesIs Differentiation of Rat Osteoblast Cultured. Herald of Medicine, 2019, 38(6): 730-734.
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