1.Department of Immunology，Tongji Medical College, Huazhong University of Science and Technology，Wuhan 430030，China；2.Department of Clinical Laboratory，the First Hospital of Xiamen City，Fujian Province 361000，China
Abstract：To produce and express a biralent siglechain Fv(BsFv) specifically binding to liver cancer cells，and to identify its biologic activity. MethodsPCR primers were designed to introduce interlinker G4S and the restriction endonuclease AscI，and two fragments were produced from the parent scFv SLH10 by PCR，and then linked into the expression vector pAB1. The cellbinding activities of the BsFv and scFv were analyzed by FACS using some cell lines. ResultsBsFv was found to bind to selected cell lines more specifically than parent scFv. ConclusionBsFv is successfully constructed with prominent superiority to scFv，laying a foundation for its use in the diagnosis and treatment in clinical tumors.