Archive

• 01 September 2014 Volume 33 Issue 9

    

  ---

  药物研究
  麻醉科用药专栏
  药物与临床
  药物制剂与药品质量控制
  用药指南
  

• Select all
  |

药物研究
• Select
  药物研究

  Establishment of a TMZresistant Human Glioma Cell Line U251/TR and the Mechanism of Drugresistance

  PAN Qiang;YANG Xuejun;Gao Song;JI Yanwei;ZHANG Wengao

  2014, 33(9): 1121-1125.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo establish a drugresistance cell line of human glioma with temozolomide (TMZ),investigate its resistance mechanisms,and provide experimental evidence for optimal TMZ therapy.MethodsA TMZresistant human glioma cell line,U251/TR,was established by stepwise exposure of human parental U251 cells to TMZ.Resistance index and cell viability were accessed by MTT assay.WesternBlot,RTPCR,immunohistochemistry and immunofluorescence were used to detect MGMT expression for the analysis of resistance mechanism.ResultsA TMZresistant human glioma cell line,U251/TR,was developed after 8 months of stepwise induction with 0.25－16.00 μg?mL－1 TMZ.IC50 in U251/TR cells was approximately 7 times higher compared with that in U251 cells (P＝0.00 ).The MGMT expression was significantly increased in U251/TR cells compared with that in parental U251 cells （P＝0.00） .ConclusionA TMZresistant human glioma cell line,U251/TR,was established by stepwise exposure of human parental U251 cells to TMZ.The primary mechanism of TMZ resistance is associated with increased activity of MGMT.
• Select
  药物研究

  Protective Effects of Acanthoic Acid on Acute Liver Injury in Mice

  HAO Chengyi;WU Yanling;LIAN Lihua;NAN Jixing

  2014, 33(9): 1126-1128.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo study protective effects of acanthoic acid (AA) against acute liver injury.MethodsSixty mice were randomly divided into six groups (n＝10 each),including normal control group,model control group,positive control group (Nacetyl L cysteine,NAC,300 mg?kg－1),and AA at small (50 mg?kg－1),middle (100 mg?kg－1),and high (200 mg?kg－1) dose groups.Each group received respective treatment for 3 days and fasted for 16 h before the last dose.All animals except of the normal control group were treated with tacrine (35 mg?kg－1) 1 h after the treatments.Hepatic pathological and serum biochemical changes were observed.ResultsThe highdose of AA significantly reduced the levels of AST (143±46) U?L－1，ALT (32±9) U?L－1，LDH (1 218±312) U?L－1,MDA (3.24±0.48) μmol?g－1,and GSH (417±15) mg?g－1 compared with the model control group (P＜0.05 or P＜0.01).Liver injury was also ameliorated in AA high dose group.ConclusionAA has a protective effect on acute liver injury in mice.
• Select
  药物研究

  BQ123 Alleviates Lung Injury Following Limb Ischemia-Reperfusion in Rats

  LIU Yan;PENG Haibing;WANG Jianhui;WANG Yinhuan;ZHANG Na;LI Ying;ZHU Liyan;YANG Xiu

  2014, 33(9): 1129-1133.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo observe BQ123 on lung tissue injury following ischemiareperfusion of hind limbs in rats.MethodsA total of 30 male SD rats were randomly divided into control,IR,and BQ123 groups,with 10 rats in each group.After 4 hour of ischemia and 4hour of reperfusion to the hind limbs,ET1 was measured by radioimmunoassay,levels of myeloperoxidase (MPO) and malondialehyde (MDA) were examined by biochemical method,and the content of Pselectin was examined by ELISA.Immunohistochemical method was used to detect the expressions of Fas,Bcl2 and Caspase3 in lung tissues.Pulmonary apoptosis was examined by means of TdTmediated dUTP nick end labeling (TUNEL).ResultsCompared with the control group,the levels of ET1,MPO,MDA and Pselectin in lung tissues were all increased significantly in IR group (P＜0.01).The expressions of Fas,Bcl2 and Caspase3 were 0.294±0.003,0.108±0.005,and 0.174±0.003,significantly upregulated in the IR group.The apoptosis rate ［(18.83±2.86)%］ was significantly increased in the IR group (P＜0.01).Compared with IR group,the tissue contents of ET1,MPO,MDA and Pselectin were significantly lowered in BQ123 group (P＜0.01).The expression levels of Fas (0.115±0.007) and Caspase3 (0.159±0.006) were decreased,but the expression of Bcl2 was increased (0.128±0.005).The apoptosis rate in BQ123 group was significantly lower ［(10.67±2.16)%,P＜0.01］.ConclusionBQ123 may have protective effects on lung tissue after limb ischemiareperfusion in rats by means of improving neutrophil aggregation and reducing the expression of proteins related to cell apoptosis.
• Select
  药物研究

  Tumor Inhibition of AcidResponsive PolymerCisplatin Conjugate Nanoparticles Drug Delivery System

  LIANG Tao

  2014, 33(9): 1132-1136.

  Download PDF ( )   Knowledge map   Save

  Objective To synthesize polymercisplatin conjugate nanoparticles for acidresponsive drug delivery,and verify the inhibitory effect of the nanosystem on cancer cells.MethodsAcidresponsive polymercisplatin conjugate nanodrug carrier was synthesized to simulate the drug release under an acidic environment.Cell viability was examined by MTT assay.ResultsThe polymercisplatin nanoparticles exhibited wellcontrolled cisplatin loading rate,and excellent acidresponsive drug release kinetics.At pH 7.4, little drug was released.At pH 5.0 or 6.0,the drug release was significant.Compared with free cisplatin,cisplatin in the conjugate nanoparticles showed higher cytotoxicity against ovarian cancer cells in vitro.An equivalent dose of 7 μmol?L－1 cisplatin loaded in the conjugate nanoparticles exhibited same cytotoxicity as 50 μmol?L－1 free cisplatin.ConclusionPolymercisplatin conjugate is an acidresponsive drug delivery system that greatly improves the bioavailability of cisplatin to tumor cells.It is expected to play a better tumor inhibition effect by quickly releasing a high dose of chemotherapy drug inside tumor cells.
• Select
  药物研究

  Protective Effects of Schisandra Lignans Against DoxorubicinInduced Liver Injury in Rats

  AN Ying;WANG Yanchun;XU Lu;FAN Hongyan;SHEN Nan;ZHAO Lijing;CHEN Xue;XU Bo

  2014, 33(9): 1136-1139.

  Download PDF ( )   Knowledge map   Save

  
  ABSTRACT Objective To investigate protective effects of schisandra lignans on doxorubicininduced liver injury rats.MethodsSD rats were randomly divided into six groups: normal control,model control, vitamin C and schisandra lignans at small, middle,and high doses.The liver injury model was established by intraperitoneal injection of doxorubicin.Normal controls were intragastrically administrated with 0.9%sodium chloride solution,while other groups were administrated with different medications,respectively. Interleukin10 (IL10) in serum,nitric oxide (NO) and aspartate aminotransferase (AST) in liver tissue homogenate were measured.Histopathological changes in hepatic tissues were also observed.ResultsSerum IL10 and tissue NO were obviously lower in the model control group than those in other groups,while those in all schisandra lignans treated groups were significantly improved (P＜0.05, P＜0.01).The AST level in schisandra lignans middle and high dose groups was (372.58±105.80) and (327.92±42.80) U?g－1,respectively,significantly lower than （565.07±22.13） U?g－1 in the model control group (P＜0.05,P＜0.01).Histopathological analysis showed that degeneration and necrosis of hepatocytes were remarkably alleviated in all schisandra lignans treated groups.ConclusionSchisandra lignans protect rats against doxorubicininduced liver injury.
  KEY WORDS
• Select
  药物研究

  Effects of Total Flavonoids from Portulaca on Transforming Growth Factor β1 in Rats with Hepatic Fibrosis

  PAN Xiaoli;XIONG Yongai;TAN Yuzhu;XIANG Hui

  2014, 33(9): 1140-1143.

  Download PDF ( )   Knowledge map   Save

  
  ABSTRACT Objective To explore the effects of total flavonoids from portulaca against liver fibrosis in rats by detecting TGFβ1 gene and protein expressions.MethodsA total of 48 SD rats were randomly divided into normal control, model control, glucyrrhizin aqueous,and total flavonoids groups,with 12 rats in each group.Except those in the normal control group,rats in other groups were intraperitoneally injected with 2 mL?kg－1?d－1 carbon tetrachloride to induce liver fibrosis.Rats in glucyrrhizin aqueous group and total flavonoids ones were intragastrically administered with 15.75 mg?kg－1 of glucyrrhizin aqueous solution or 35.6 mg?kg－1 of total flavonoids aqueous solution,respectively.The normal and model control groups were administered with equal volume of aqueous solution.Thirty days later,rats were sacrificed by anesthesia.Livers were obtained to detect TGFβ1 gene and protein expressions by RTPCR and WesternBlot.ResultsRelative gene expression of TGFβ1 in the normal control,model control,glucyrrhizin aqueous and flavonoids groups was 0.725±0.130,7.493±1.410,3.016±1.240,and 2.668±1.150,respectively.Total flavonoids from portulaca significantly reduced the gene (P＜0.01) and protein (P＜0.01) expressions of TGFβ1.ConclusionEfficacy of total flavonoids from portulaca in treating hepatic fibrosis may be related to decreased TGFβ1 expression in rats.
  KEY WORDS Total flavonoids from portulaca; Liver cirrhosis; Transforming growth factors
  
• Select
  药物研究

  Comparison of Antiinflammatory and Hepatoprotective Activities of Bupleurum marginatum Wall.ex DC.and Bupleurum chinense DC.

  DU Ting;DU Shiming;WANG Gang;SUN Rongjin;YANG Guangyi;YE Fang;YUAN Lingling;LIU Tao

  2014, 33(9): 1144-1149.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo compare antiinflammatory and hepatoprotective effects of Bupleurum marginatum Wall.ex DC.and Bupleurum chinense DC.MethodsThe antiinflammatory effect of two kinds of radix bupleuri decoction were compared using the xyleneinduced ear edema mice model and the egg whiteinduced foot swelling rats model.Acute liver injury in mice was induced through intraperitoneal injection of peanut oil with 0.1%carbon tetrachloride.Alanine aminotransferase (ALT),aspartate aminotransferase (AST),and alkaline phosphatase (ALP) in serum,superoxide dismutase (SOD),malondialdehyde (MDA),and glutathione (GSH) in liver tissue homogenate were detected.Histopathological changes,transforming growth factorβ (TGFβ) and nuclear factorκB (NF κB) in hepatic tissues were also observed.ResultsEar swelling experiment showed that the decoction of both Bupleurum marginatum Wall.ex DC.and Bupleurum chinense DC.had equal antiinflammatory effects (P＞0.05).Similarly,the decoction of Bupleurum marginatum Wall.ex DC.and Bupleurum chinense DC.improved levels of ALT,AST,SOD,GSH,and MDA to the same extent compared with the model control group (P＜0.05 or P＜0.01).There was no significant difference among all groups in ALP levels (P＞0.05).Histopathological and immunohistochemical experiments showed that expressions of TGFβ and NF κB were similar between the two kinds of radix bupleuri.ConclusionBupleurum marginatum Wall.ex DC.and Bupleurum chinense DC.have similar antiinflammatory and hepatoprotective effects.
  KEY WORDS
• Select
  药物研究

  Study on Pharmacokinetics and in Vitroin Vivo Correlation of Riluzole Tablets in Dogs

  MU Jinchao;LIU Chunfen

  2014, 33(9): 1149-1153.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo study the pharmacokinetic parameters of riluzole tablets and commercial riluzole capsules in Beagle dogs,and evaluate the correlation between the release rate in vitro and the absorption in vivo,and calculate the relative bioavailability of riluzole tablets.MethodsSix Beagle dogs were treated with 50 mg of riluzole tablets or capsules,and then crosstreated by the other drug after 14days wash out period.Blood concentration of riluzole was measured by high performance liquid chromatography (HPLC).The atrioventricular model was used to calculate the pharmacokinetic parameters and WangerNelson method was applied to assess the correlation between the release rate in vitro and the absorption in vivo.ResultsThe t1/2,tmax,Cmax,AUC072 and AUC0∞ in the tablet and capsule groups were (12.43±3.87) and (12.57±3.25) h,(6.00±2.60) and (6.23±2.72) h,(56.24±16.51) and (60.82±18.13) ng?mL－1,(1 255.83±311.39) and (1 283.50±313.81) ng?mL－1?h,(1 282.57±322.64) and (1 297.22±297.39) ng?mL－1?h,respectively.The relative bioavailability of capsules versus tablets was (105.9±30.6)%.ConclusionHPLC method can be applied to measure the concentration of blood riluzole with little interference and high levels of repeatability and accuracy.The absorption in Beagle dogs between riluzole tablets and capsules was equal,and a good correlation between the in vivo absorption and in vitro release has been found in this study.
  KEY WORDS Riluzole; Pharmacokinetics; Chromatography, high performance liquid
麻醉科用药专栏
• Select
  麻醉科用药专栏

  Comparative Study of Propofol and Isoflurane on Inflammatory Cytokines in Patients with Intracranial Neoplasm

  XU Lufeng;PAN Wen;GONG Jian;GUO Feng;FENG Rui;CUI Wenyao;LV Fubin;GAO Chengjie

  2014, 33(9): 1154-1156.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo explore the neuroprotective mechanism of propofol by comparing the influence of propofol and isoflurane on inflammatory cytokines (TNFα、IL1、ICAM1 ) in patients with intracranial tumors.MethodsOne hundred and sixtyeight patients with intracranial neoplasm were randomly divided into two groups: the propofol (Group P) and isoflurane (Group I),84 cases in each.Patients were given with propofol (3－6 μg?mL－1) by plasma targetcontrolled infusion or with continuously inhaled isoflurane (1%－2%),respectively.The serum levels of TNFα,IL1 and ICAM1 were detected before anesthesia and at 0,24,and 48 h after operation.ResultsThe serum levels of TNFα,IL1 and ICAM1 were significantly increased after operation as compared to baseline in both groups.The serum level of TNFα was（69.11±8.95） and （76.26±11.28） μg?mL－1,IL1 was（21.57±3.19） and （29.58±4.38） ng?L－1,and ICAM1 was （1.63±0.24）and （1.94±0.29） g?L－1 at 24 h post operation in Group P and Group I,respectively. These inflammatory cytokine levels were significantly higher in group I compared to group P at 24 and 48 h after operation (P＜0.05 or P＜0.01).ConclusionThe targetcontrolled infusion of propofol brings about lower level of inflammatory reaction than isoflurane inhalation in patients with intracranial neoplasm,which may attribute to the mechanism of brain protection against injury.
  KEY WORDS Propofol; Isoflurane; Neoplasms,nervous tissue; Tumor necrosis factor; Interleukin; Intercellular adhesion molecular
  
• Select
  麻醉科用药专栏

  Effect of Sufentanil Combined with Dexmedetomidine on Hemodynamic and Narcotrend Index During Pediatric Anesthesia Induction

  QI Xiang;LIANG Zhi;LU Huaihai;BO Lijun;GAO Luchao;XU Xue

  2014, 33(9): 1157-1161.

  Download PDF ( )   Knowledge map   Save

  Objective To investigate the effect of different doses of sufentanil combined with dexmedetomidine (DEX) on hemodynamic and Narcotrend index (NI) during pediatric anesthesia induction.MethodsA total of 45 children with lower abdominal surgery were randomly divided into three groups evenly: sufentanil 0.1 μg?kg－1+ DEX (S1 group),sufentanil 0.2 μg?kg－1+DEX (S2 group),and sufentanil 0.3 μg?kg－1+ DEX (S3 group).Patients in each group began with intubation at the peak point of administration.Blood pressure,heart rate,perfusion index (PI) and NI were detected at the baseline (t0),delivering DEX 0.5 μg?kg－1?h－1 and sufentanil intravenously for 5 min (t1),delivering sufentanil for 3 min (t2),time of intubation (t3),1 min (t4),and 5 min (t5)after intubation.The application rate of atropine and propofol was recorded.Patient recovery time and adverse reactions were observed.ResultsCompared with baseline value at t0 time point,hemodynamic parameters and NI were decreased at t1 and t2,while PI was increased in both groups.At t3,t4,and t5,all of the indicators in S1 group were significantly different from those at t0,and also significantly different from those in S2 and S3 group. Six patients were treated with propofol in S1 group and four presented with agitation after operation,more than S2 and S3 groups.Three patients were treatment with atropine in S3 group.ConclusionSufentanil (0.2 μg?kg－1) combined with dexmedetomidine can be used to induce intubation for pediatric anesthesia with stable hemodynamic profile and low incidence of adverse effects.
  KEY WORDS Sufentanil; Dexmedetomidine； Pediatrics; Anesthesia induction; Narcotrend index
• Select
  麻醉科用药专栏

  Neuroprotective Effect of Sevoflurane on Controlled Hypotension in Patients with Coronary Heart Disease Undergoing Craniocerebral Surgery

  YANG Yunzhao;LI Jin;FENG Xinmin

  2014, 33(9): 1161-1166.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo study the neuroprotective effect of sevoflurane on controlled hypotension in patients with coronary heart disease undergoing craniocerebral surgery.MethodsTwentysix patients with coronary heart disease undergoing craniocerebral surgery were randomly divided into two groups,receiving either inhaled 2%－5%sevoflurane plus intravenous sodium nitroprusside (treatment group,n=13) or intravenous sodium nitroprusside 3－8 μg?kg－1 alone (contorl group,n=13) for blood pressure control.The hemodynamic changes were recorded during the operation.Patient satisfaction with surgeons and the duration hospital stay after surgery were recorded.The levels of cardiac troponin I (TNI),creatine kinaseMB (CKMBM),neuron specific enolase (NSE) and S100 calcium binding protein β (S100β protein) in serum were detected at one day preoperation,the end of the operation,and one day postoperation.ResultsThe duration of hospital stay after surgery was significantly shortened in treatment group ［（20.3±3.8） versus （23.9±4.6） d,P＜0.05) compared with control group.The average heart rate significantly decreased,surgeon satisfaction significantly increased,and serum levels of NSE and S100β protein one day postoperation significantly decreased as compared to control group (all P＜0.05).Compared with the day before operation,serum levels of NSE and S100β protein in the two groups increased significantly at the end of surgery (P＜0.05),and the levels of TNI,CKMBM,NSE and S100β significantly elevated one day postoperation (P＜0.05).Compared with the end of operation,serum levels of NSE and S100β in contorl group increased remarkably (P＜0.05)ConclusionSevoflurane plays an important neuroprotective role,as evidenced by improving surgeon's satisfaction,reducing hospital stay after surgery,and maintaining the balance of myocardial oxygen delivery and consumption in craniocerebral surgery patients with coronary heart disease during controlled hypotension.However,it can not prevent postoperative myocardial injury in these patients.
  KEY WORDS Sevoflurane; Sodium nitroprusside; Hypotension,controlled; Neuroprotection; Calciumbinding proteins; Neuron specific enolase
  
药物与临床
• Select
  药物与临床

  Effect of Quetiapine on Brainderived Neurotrophic Factors in Patients with Firstepisode Schizophrenia

  LIU Jianjun;SUN Jushui;SHEN Xinhua;ZHONG Hua;GUO Weigang;ZHI Shengli;SONG Guangming;XU Qiuxia

  2014, 33(9): 1185-1187.

  Download PDF ( )   Knowledge map   Save

  
  ABSTRACT ObjectiveTo investigate the effects of quetiapine on serum levels of brainderived neurotrophic factors (BDNF) and the correlation between BDNF and psychiatric symptoms and cognitive function in patients with firstepisode schizophrenia.MethodsEighty patients with firstepisode schizophrenia (treatment group) were treated with quetiapine orally for 4 weeks,at initial dose of 100 mg?d－1 and average dose of (580±120) mg?d－1.The psychiatric symptoms were evaluated by using the positive and negative syndrome scale (PANSS).The cognitive function was assessed by using Wisconsin cards sort test (WCST).The serum BDNF level was detected with enzymelinked immunosorbent assay (ELISA).ResultsThe serum level of BDNF was markedly lower in schizophrenic patients before［(13.72±8.79) ng?mL－1,P＜0.01］ and after treatment［(18.02±9.06) ng?mL－1,P＜0.05］in comparison with normal controls(23.67±10.13) ng?mL－1］.After treatment,the PANSS total scores and subscale scores decreased,WCST number of categories and the number of correct answers increased,and the number of wrong answers reduced.There was a positive correlation between the serum BDNF and negative symptoms (SANS) (r= 0.54,P＝0.032),and the number of correct answers.ConclusionThe quetiapine significantly increases serum level of BDNF in schizophrenia patients,which correlates positively with improvements in symptoms and cognitive function.
  KEY WORDS Quetiapine; Schizophrenia; Neurotrophic factors,brainderived; Cognitive function
  
药物制剂与药品质量控制
• Select
  药物制剂与药品质量控制

  Optimization for Extracting Polysaccharide from Paris polyphylla Smith var.chinensis（Franch.）Hara in Shaan'xi by Response Surface Method

  LIU Yun;ZHAO Peng;SONG Xiao;WANG Changli

  2014, 33(9): 1212-1215.

    Knowledge map   Save

  ABSTRACT ObjectiveTo select the optimum extracting method of polysaccharide from Paris polyphylla Smith var.chinensis（Franch.）Hara in Shaan'xi.MethodsThe optimum extraction technology and condition were determined by using response surface methodology and single factor experiment,with the extraction ratio of polysaccharides from Paris polyphylla Smith var.chinensis（Franch.）Hara in Shaan'xi as indicator.ResultsThe optimum extraction condition for the polysaccharides was as follows: extracting at 92 ℃ for 2.6 h and with the liquid to solid ratio of 15 (mL：g).The average extraction yield of polysaccharide was 3.22%under the optimum extraction condition.ConclusionThe method is suitable for the extraction and determination of polysaccharides from Paris polyphylla Smith var.chinensis（Franch.）Hara in Shaan'xi.
  KEY WORDS Paris polyphylla Smith var.chinensis（Franch.）Hara in Shaan'xi; Polysaccharides; Response surface methodology optimization; Extraction
  
• Select
  药物制剂与药品质量控制

  Preparation of Paliperidone Long-acting Suspension for Injection

  NING Wenqi;LI Dan

  2014, 33(9): 1216-1219.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo prepare the paliperidone longacting suspension for injection and study the quality of it.MethodsThe optimum formula was measured by using orthogonal design method regarding the content of paliperidone,NaCMC,mannitol and the ground time as variable factors.Orthogonal design table L9（34） was used in the experiment.The paliperidone longacting suspension for injection was prepared by grinding the dispersion,and its quality was evaluated.ResultsThe optimum prescription was as follows: using paliperidone 40 g,NaCMC 15 g,mannitol 60 g,purified water 1 000 mL,appropriate amount of NaH2PO4 and NaOH,and grinding for 1 h.Paliperidone longacting suspension for injection was characterized by an average pH of 7.0,and ratio of average sedimentation volume of 90.3%.The suspension released smoothly within 1 h in vitro and achieved sustained release.The suspension was stable in a freezedried powder.ConclusionThe preparation for paliperidone longacting suspension for injection is simple and stable,which may be a novel drug delivery system.
  KEY WORDS Paliperidone; Longacting; Suspension
• Select
  药物制剂与药品质量控制

  Content Variation of Bupleurum Polysaccharides from Bupleurum marginatum at Different Collecting Periods in Northwest of Hubei Province

  YE Fang;YANG Guangyi;DU Shiming;WANG Gang;DU Ting;SUN Rongjin

  2014, 33(9): 1219-1221.

  Download PDF ( )   Knowledge map   Save

  
  ABSTRACT ObjectiveTo study the dynamic change of polysaccharides from Bupleurum marginatum in Northwest of Hubei province,and to explore the optimal harvest period for Bupleurum marginatum.MethodsThe contents of soluble and crude Bupleurum polysaccharides were determined by anthronesulphuric acid colorimetric method and detected at 620 nm,and total polysaccharide content was calculated.ResultsThe content of polysaccharides at various collecting periods was very different from 9.642% to 15.479%,and reached the highest level in November when the seed comes to mature.ConclusionThe optimal harvest period for Bupleurum marginatum in Northwest of Hubei is at the end of November.
  KEY WORDS Bupleurum marginatum Wall.ex DC.; Northwest of Hubei province; Polysaccharides; Collecting periods
  
• Select
  药物制剂与药品质量控制

  Content Determination of Rutin in Stems,Leaves,and Fruits of Sambucus williamsii Hance by HPLC

  HU Yanwu;GUAN Yingli;ZHANG Liwei;WANG Zhaohui

  2014, 33(9): 1222-1224.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo determine the content of rutin in fruits,stems,and leaves of Sambucus williamsii Hance,and provide an essential reference for the exploitation and utilization of the resources.MethodsThe content of rutin was analyzed on an Agilent ZORBAX Eclispse XDB C18 column(150 mm×4.6 mm,5 μm).The mobile phase was methanol0.1%phosphoric acid (45：55),the column temperature was set at 25 ℃,the flow rate was 0.8 mL?min－1,and the detection wavelength was set at 360 nm.ResultsThe linear range of rutin was 0.105－0.525 μg (r＝0.999 6),the average recovery was 97.86%,and RSD was 0.49%.The content of rutin in stems,leaves,and fruits of Sambucus williamsii Hance was 0.171,0.522,and 0.165 mg?g－1,respectively.ConclusionThe stems,leaves and fruits of Sambucus williamsii Hance all contain rutin,but at a significant different content level.The leaves contain the highest level of rutin.
  KEY WORDS Sambucus williamsii Hance; Rutin; Chromatography, high performance liquid
  
• Select
  药物制剂与药品质量控制

  Simultaneous Determination of Icariin and Naringin in Bushen Huayu Extract by HPLC

  OUYANG Lu;XIA Yong;XIA Peng;LI Hongliang;WANG Xuanbin;

  2014, 33(9): 1224-1226.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo establish a method for simultaneous determination of icariin and naringin content in bushen huayu extract by HPLC.MethodsReversephase high performance liquid chromatography (RPHPLC) separation was performed one C18 column (4.6 mm×250 mm,5 μm).The mobile phase was acetonitrile containing 0.2%H3PO4 (pH＝2.9).Gradient elution with a flow rate of 0.8 mL?min－1 was applied to achieve the separation.The detection wavelength was set at 269 nm,and the column temperature was 30 ℃.ResultsIcariin had a good linear range from 0.3－3.0 μg (r＝0.999 9). The recovery rate was 105.6%,and RSD was 0.95%.Naringin was linear within the range of 0.12－1.20 μg (r＝0.999 9).The recovery rate was 94.0%and RSD was 0.52%.ConclusionThe method is simple,stable,accurate,and reproducible,which can be used as the quality control standard for bushen huayue extract.
  KEY WORDS Bushen huayu extract; Icariin; Naringin; Chromatography, high performance liquid

• Select
  药物制剂与药品质量控制

  Comparation of Total Iridoids in Valerian from Different Habitats

  YUAN Xiuzhi;XU Mingwang;XUE Cunkuan

  2014, 33(9): 1227-1229.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo determine and compare the contents of total iridoids in valerian from different origins.MethodsThe total iridoids in valerian was detected by reaction with alkaline hydroxylamineperchloric acid iron reagent，and the absorbance was measured at 522 nm by spectrophotometry.ResultsThe content of valepotriate and its absorption were linear within the range of 0.05－1.40 mg?mL－1.The average recovery was 98.50%，and RSD was 1.02%.There was significant difference between the contents of iridoid in valerian from different habitats.ConclusionThis method is accurate and reliable for evaluating the quality of valerianand.The contents of valeriana is related to its habitats.
  KEY WORD SValerian; Total iridoids; Valepotriate; Spectrophotometry
用药指南
• Select
  用药指南

  Effectiveness and Safety of Recombinant Human Endostatin Combined with Concurrent Chemoradiotherapy for Advanced Nonsmall Cell Lung Cancer: a MetaAnalysis

  XU Huilin;GE Wei;CAO Dedong;MING Pingpo;ZHENG Yongfa;SONG Jing;LUO Wei

  2014, 33(9): 1237-1242.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo evaluate the effectiveness and safety of recombinant human endostatin combined with concurrent chemoradiotherapy versus concurrent chemoradiotherapy for advanced non small cell lung cancer (NSCLC).MethodsElectronic databases including the Cochrane library,PubMed,the Chinese biomedical literature database,China national knowledge internet(,EMbase,VIP and Wanfang database system were searched,until August,2013.The inclusion criteria was efficacy and safety studies of randomized controlled clinical studies in which recombinant human endostatin combined with concurrent chemoradiotherapy was compared with concurrent chemoradiotherapy alone for patients with advanced NSCLC.Cochrane handbook 5.1.0 was applied in evaluating the quality of included trials and RevMan 5.1.0 software was used for data analysis.ResultsFive studies including 217 cases of advanced NSCLC were included.The results of the metaanalysis exhibited that compared with concurrent chemoradiotherapy alone,recombinant human endostatin combined with concurrent chemoradiotherapy could increase effective rate ［OR＝2.62,95%CI（1.41,4.86）,P＝0.002］.But there were no significant differences in clinical benefit rate ［OR＝2.08,95%CI（0.92,4.73）,P＝0.08］,one year survival rate ［OR＝1.18,95%CI（0.53,2.66）,P＝0.68］,improvement in quality of life ［OR＝1.57,95%CI（0.40,6.07）,P＝0.52］,rate of leucopenia ［OR＝1.25,95%CI（0.72,2.17）,P＝0.43］,radioactive esophagitis ［OR＝1.16,95%CI（0.42,3.21）,P＝0.77］ and radiation pneumonitis ［OR＝2.47,95%CI（0.34,17.68）,P＝0.37］.ConclusionCompared with concurrent chemoradiotherapy alone,recombinant human endostatin combined with concurrent chemoradiotherapy may be more effective for advanced NSCLC,whereas improvement of life quality and toxicities are similar.For the quality restriction and possible publication bias of the included studies,more high quality randomized controlled trials are required to further verify this conclusion.
  KEY WORDS Recombinant human endostatin; Lung neoplasms; Concurrent chemoradiotherapy; Metaanalysis