中国科技论文统计源期刊 中文核心期刊  
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊  
日本科学技术振兴机构数据库(JST)
第七届湖北十大名刊提名奖  

Archive

  • Select all
    |
    药物研究
  • 药物研究
    ZHOU Min;CHEN Hui-long;CHENG Sheng;ZHANG Hui-lan;XIE Min;XIONG Wei-ning;XU Yong-jian
    2013, 32(10): 1263-1266. https://doi.org/10.3870/yydb.2013.10.002
    Objective To observe the effects of aminophylline and doxofylline on the expression of AGR2 and Muc5ac protein in lung tissue in asthmatic mice,and to investigate the role of doxyfylline in asthma airway mucus hypersecretion.Methods Thirtytwo female mice were randomly divided into normal control group,asthmatic group,aminophylline group and doxofylline group.The expression levels of AGR2 and Muc5ac protein in lung were detected by immunohistochemical Methods .Interleukin13 level in bronchoalveolar lavage fluid (BALF) was analyzed by ELISA.Results The expression levels of AGR2 protein (0.544±0.039) and Muc5ac protein (0.556±0.045) in asthmatic group were significantly higher than those in normal control group (0.388±0.053 and 0.188±0.086,respectively,P<0.01).The expression levels of AGR2 (0.491±0.029,P<0.05) and Muc5ac protein (0.446±0.044,P<0.01) in doxofylline group were significantly decreased as compared with asthmatic group.KEY WORDS Doxofylline may downregulate the expression of AGR2 and Muc5ac protein in asthmatic mice and alleviate airway mucus hypersecretion.
  • 药物研究
    HE Bing-bing;LIU Pu-qing;CHEN Feng
    2013, 32(10): 1266-1269. https://doi.org/10.3870/yydb.2013.10.003
    Objective To compare the impacts of 6% hydroxyethyl starch (HES) from different enterprises on renal function of rats with mild blood loss.Methods Sixty healthy SD rats were equally divided into 6 groups.One group received no treatment (Group C).Mild blood loss model was established in the other 5 groups by producing 20% hemorrhage.The rats were administered with normal saline (Group M),HES200/0.5 (Group H1),HES200/0.5 (Group H2),HES130/0.4 (Group H3),and HES130/0.4 (Group H4) by caudal vein injection.The above treatments were repeated every 24 hours for seven days.Blood samples were collected to determine the levels of serum creatinine (Cr) and blood urea nitrogen (BUN).The rats were sacrificed,and their kidneys were taken out for SOD,MDA detection and pathological examination.Results The degree of renal function impairrment and tissue injury was higher in H1 group than in H2 group,higher in H3 than in H4 group,and higher in H2 than in H4 group.KEY WORDS The research shows that different pharmaceutical companies may have different manufacturing facilities,quality standards and management system,which Results in the differences of drug quality between different pharmaceutical companies.The higher the HES molecular mass and replacement degree are,the greater the injury of HES to renal tissues is.It may be because that the relative larger molecular mass HES stays longer in the kidney to lead to more serous injury.
  • 药物研究
    QIU Hong;ZHANG Ming-Sheng
    2013, 32(10): 1270-1273. https://doi.org/10.3870/yydb.2013.10.004
    Objective To synthesize polymerizable antimicrobial monomer through a silanebased solgel route and characterize the polymerization properties of bis-GMA resin incorporated with aforementioned monomer. Methods Tetraethoxysilane and trialkoxysilanes were used as precursors to synthesize partially hydrolyzed quaternary ammonium methacryloxy silicate (QAMS-3PH). The monomer conversion and cure shrinkage of bis-GMA resin were characterized.Results QAMS-3PH decreased the polymerization shrinkage of bis-GMA resin, without affecting the monomer conversion. Results QAMS-3PH, as a diluent monomer for bis-GMA resin, may contribute to improved mechanical properties of bis-GMA resin system.
  • 药物研究
    CAO Yong;CHEN Hui-long;FANG Hui-juan
    2013, 32(10): 1274-1277. https://doi.org/10.3870/yydb.2013.10.005
    Objective To study the expression of cysteinerich 61 (cyr61) protein in asthmatic mice and the influence of aminophylline on it.Methods Forty BALB/c mice were divided into asthma group (n=10),aminophylline group (n=10),asthma control group (n=10) and normal group (n=10).The asthma mouse model was established by ovalbumin (OVA)sensitization and challenge.Aminophylline group was administered with aminophylline into the tail vein after OVA challenge.Asthma control group was administered with normal saline.The expression of cyr61 protein in the lung tissues was detected by immunohistochemistry.The level of interleukin4 protein was determined in the bronchoalveolar lavage fluid (BALF) by enzymelinked immunosorbent assay (ELISA).The count of inflammatory cells in BALF was also analyzed.Results The results showed that the expression of cyr61 protein in asthma group was significantly higher than that in normal mice (P<0.05).The expression level of cyr61 was significantly decreased in aminophylline group as compared with asthma group (P<0.05),though it was still higher than that in normal mice (P<0.05).The cyr61 protein expression correlated positively with the total caryocytes (P<0.05),eosinophils (P<0.05),lymphocytes (P<0.05) and IL-4 level (P<0.05) in the BALF.Conclusion Our findings suggest that cyr61 might contribute to the airway inflammation in asthma.
  • 药物研究
    YAN Ming;ZHAO Bing-hai;ZHANG Chun-lei;LIU Jie-ting;LI Hong-zhi;YUAN Xiao-huan;CHU Yan-hui;WANG Bao-qing
    2013, 32(10): 1278-1281. https://doi.org/10.3870/yydb.2013.10.006
    Objective To study the effect of baicalin on injured human SH-SY5Y neuroblastoma cells induced by H2O2 and the related mechanism. Methods SH-SY5Y cells were pretreated with different concentrations of baicalin for 24 h. The cells were then treated with 200 μmol.L-1 H2O2 for 24 h. Realtime fluorescent quantitative PCR was used to determine thioredoxin protein (Trx) mRNA expression. ELISA was used to determine the Trx protein expression. Results Baicalin in the 50-300 μmol.L-1 concentration range exhibited no cytotoxic effect to SH-SY5Y cells. The cell survival rate was (0.410±0.096), (1.304±0.101), (0.889±0.089), and (0.609 ± 0.023) after treatment with 0, 50, 100 and 200 μmol.L-1of baicalin, respectively. The higher dose groups (50, 100 and 200 μmol.L-1 ) had significantly higher survival than the H2O2 injured group (0.339±0.073). The normal group, H2O2 injured group, 50,100 and 200 μmol.L-1 baicalin treatment group had Trx mRNA expression level of (1.203±0.014), (0.021±0.004), (0.054±0.005), (0.071±0.013) and (0.042±0.004), respectively. The Trx protein expression level was (26.230±0.857), (19.230±0.982) (22.440±0.888), (23.020±1.070) and (22.330±1.067), respectively.Conclusion Baicalin has protection effect on H2O2injured SH-SY5Y cells, likely due to resistance to oxidative stress and apoptosis through downregulation of Trx expression.

  • 药物研究
    LI Juan;ZHAO Ying;WANG Gui-ling;FEI Hong-rong
    2013, 32(10): 1281-1284. https://doi.org/10.3870/yydb.2013.10.007
    Objective To detect the effect of perifosine,an inhibitor of phosphoinositol3-kinase/Akt,on the cell growth of human osteosarcoma U2-OS cells,and to study the molecular mechanism of the synergistic antiproliferative effect of perifosine combined with cisplatin on U2-OS cells.Methods Cell growth inhibition was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.AnnexinVFITC apoptosis detection kit was used to detect cell apoptosis.Protein expression was examined by Western blotting.Results Akt phosphorylation was inhibited by perifosine in U2-OS cells.MTT Results indicated that perifosine inhibited the growth of U2-OS cells in a dosedependent manner.Combined utilization of perifosine substantially increased cytotoxic effects of cisplatin on U2-OS cells and induced the cell apoptosis by activating the caspase related signaling pathway.Conclusion The PI3K/Akt inhibitor perifosine can significantly inhibit the growth of U2-OS cells.Perifosine combined with cisplatin can synergistically induce apoptosis of human osteosarcoma cells.The mechanism might be related to the co-regulation of caspases activation pathways.

  • 药物研究
    PAN Yong-li
    2013, 32(10): 1285-1288. https://doi.org/10.3870/yydb.2013.10.008
    Objective To explore the effects of piclamilast on TNF-α/NF-κB signaling in lung of rats with endotoxin (LPS)induced acute lung injury (ALI) and the related mechanism.Methods SD rats were randomly divided into five groups: control group,model group,1,2 and 4 mg.kg-1 piclamilast groups (n=10 in each group).The model of ALI was established by intratracheal instillation of LPS.Piclamilast was orally administrated 1 h before LPS.Six hours after the LPS instillation,arterial blood was collected for blood gas analysis,and then the rats were killed.The protein expression levels of tumor necrsis factor(TNF-α),TNFR1 and NF-κB p65 in lung were determined by ELISA or Western blotting.Results As compared with control group,the pH and PaO2 in arterial blood were significantly decreased in ALI group (P<0.01),and the PaCO2 were increased (P<0.01).In addition,the protein expression levels of TNF-α,TNFR1 and NF-κB p65 in ALI group were significantly higher than those in control group (P<0.01).Piclamilast increased the pH and PaO2 in arterial blood,reduced PaCO2 and inhibited the protein expression of TNF-α,TNFR1 and NF-κB p65 in lung.KEY WORDS Piclamilast has potent therapeutic effects on LPSinduced ALI rats,and the mechanism may be related to the inhibition of TNF-α/NF-κB signaling pathway.
  • 药物研究
    YU Wei-fan
    2013, 32(10): 1289-1292. https://doi.org/10.3870/yydb.2013.10.009
    Objective To study the protective effect of total flavones of Herba verbenae (TFH) against carbon tetrachloride (CCl4)induced acute liver injury in mice.Methods Sixty mice were randomly divided into six groups: normal group,model group,positive control group,high,medium,and lowdose TFH group.The mice of normal and model groups were given normal saline,and mice of all TFH groups and positive control group were administered with TFH (50,100,200 mg.kg-1)and bifendate (200 mg.kg-1),respectively.All the mice were treated with TFH or bifendate for 10 consecutive days,once daily.One hour after the last administration of the drugs,each mouse was intraperitoneally injected with 0.15% CCl4,except the mice in the normal group.Twenty four hours after the last administration of the drugs,the activity of alanine aminotransferase (ALT),aspartate aminotransferase (AST),interleukin (IL)6,tumor necrosis factor (TNF)α,and interleukin (IL)8 in the serum,liver index,spleen index,thymus index,the expression of nuclear transcription factor (NF-κB) p65,the activities of superoxide dismutase (SOD),glutathione peroxidase (GSH-Px),catalase (CAT),and the contents of malondialdehyde (MDA),and nitric oxide (NO) in liver tissues were investigated.Results In highand mediumdose TFH groups,the serum activities of ALT and AST were significantly decreased (P<0.01).The liver index,spleen index,thymus index were significantly reduced (P<0.05 or P<0.01).The expression of NF-κB p65 was inhibited in the liver tissue,and the contents of IL-6,TNF-α,IL-8 were decreased in the serum (P<0.05 or P<0.01).The activities of SOD,GSH-Px and CAT were elevated in the liver tissue,and the content of MDA,NO were reduced (P<0.05 or P<0.01).Conclusion TFH can alleviate liver injury,protect liver cells,and reduce inflammatory exudation and oxidative injury.

  • 药物研究
    LI Xi-ping;ZHANG Cheng-liang;XU Yan-jiao;WU Tao;LIU Dong
    2013, 32(10): 1293-1295. https://doi.org/10.3870/yydb.2013.10.010
    Objective To investigate the absorption characteristics of cyclosporine A.Methods The isolated everted gut sacs of rats was used to study the absorption rate constant (Ka) in different segments of rat intestines (duodenum,jejunum,ileum,colon).The samples were collected at different time points and determined with HPLC in combination with ultraviolet detector.Results Cyclosporine A was poorly absorbed in the entire intestines.The absorption rate constant decreased from duodenum,jejunum,ileum to colon (6.646 9,4.917 9,4.262 7,2.686 1 min-1,respectively). Conclusion The absorption of cyclosporine A conforms firstorder kinetics.With high accuracy,sensitivity and specificity,the method was appropriate to investigate the absorption of cyclosporine A.
  • 药物研究
    HUANG Gui-zhi;HAO Ai-hua
    2013, 32(10): 1296-1300. https://doi.org/10.3870/yydb.2013.10.011
    Objective To synthesize Chol-PEG nano-drug carrier modified by transferrin (TF),and to research the uptake of the nano-drug carrier by C6 cells and the inhibitory effect of the nano-drug carrier on C6 cell proliferation.Methods Daunorubicin (DNR) was encapsulated in the drug-loaded nano system. The drug encapsulation efficiency and drug release of the nano-drug carrier were determined. Uptake of the nano-drug carrier by C6 glioma cells was researched by flow cytometry and competition assay. The inhibitory effect of drugloaded materials on C6 cells was analyzed by C6 cell proliferation assay. Results The nano-drug carrier system was prepared to achieve diameter less than 100 nm. The drug encapsulation rate was greater than 92%. The 72 h drug release rate was (25.25±0.52)% from the Chol-PEG package and (27.36±0.54)% from the Chol-PEG-TF package. The geometric mean fluorescence intensity was 5.55 from free DNR, 6.49 from the Chol-PEG package, and 9.51 from the Chol-PEG-TF package, respectively. After pretreatment with TF for 30 min, the mean fluorescence intensity of Chol-PEG-TF coated DNR was 6.29, as compared to 8.69 when untreated. The half inhibitory concentrations of DNR on C6 cells were (2.62±0.09), (6.56±0.55), and (1.89±0.01) μmol.L-1 from the free drug, the Chol-PEG carrier, and the Chol-PEG-TF carrier, respectively.Conclusion Chol-PEG nano-drug carrier modified by TF can improve the uptake by C6 cells and effectively inhibit the proliferation of C6 glioma cells.Therefore,it is a promissing targeted nano-drug carrier for the treatment of brain tumors.

  • 药物研究
    SONG Yan-qin;DU Yuan;SUN Xue;WANG Tian;WANG Yu-cun
    2013, 32(10): 1300-1302. https://doi.org/10.3870/yydb.2013.10.012
    Objective To study the antipyretic,anti-inflammatory and analgesic effects of An’erning granules. Methods The antipyretic,anti-inflammatory and analgesic effects of An’erning granules were evaluated in the yeastinduced rat fever model and acetic acidinduced mouse inflammation model by acetic acidinduced writhing test. Results Compared with the model group,the yeastinduced increase in body temperature was significantly less in An’erning granules medium and high dose groups 2 h after treatment (P<0.01),and the antipyretic effect lasted for at least 4 h,but An’erning granules did not exert the antipyretic effect in a dosedependent manner.The peritoneal fluid Evans blue contents were (2.7±0.5),(10.4±2.2),(7.3±3.0),(7.4±1.7),and (5.2±1.3) μg.mL-1 in control group,model group, An’erning granules low,medium,and high dose groups,respectively.Within 15 minutes,the writhing times were (33.8±13.4),(28.0±13.1),(20.5±5.0),and(18.2±7.9) in the control group,An’erning Granules low,medium and high dose groups,respectively.Conclusion An’erning granules had antipyretic,anti-inflammatory and analgesic effects.


  • 药物与临床
  • 药物与临床
    LIN Yun;XU Qing-bang;ZHANG Xue-xue;ZHANG Da-ying;WENG Ze-lin;
    2013, 32(10): 1315-1317. https://doi.org/10.3870/yydb.2013.10.019
    Objective To investigate the therapeutic effect of target radiofrequency combined with collagenase injection on recurrent lumbar disc herniation.Methods A total of 52 cases of recurrent lumbar disc herniation were enrolled.Different puncture paths were decided by scar tissue or herniated nucleus pulposus tissue gaps and forms.Patients received target radiofrequency with intradisc or intracombinedextra disc collagenase injection.Patients were followed up for 660 months (average of 18 months),and curative efficacy was evaluated by using the Macnab criteria.Results The curative efficacy was excellent in 13 cases (25.00%),good in 25 cases (48.08%),medium in 10 cases (19.23%) and poor in 4 cases (7.69%).The efficacy rated good and above reached 73.08%.Conclusion The intervention of collagenase injection combined with target radiofrequency is an effective treatment in recurrent lumbar disc herniation.
  • 药物制剂与药品质量控制
  • 药物制剂与药品质量控制
    LU Shi-hui
    2013, 32(10): 1337-1339. https://doi.org/10.3870/yydb.2013.10.026
    Objective To optimize the condition of semibiotic extraction technology for Zanthoxylum nitidum.Methods The extraction technology was optimized by orthogonal test followed by dynamic process,with extraction rate of total alkaloids and dried paste weight as the indexes.Results The optimal process was as follows: after pretreatment with cellulasepectinase,Zanthoxylum nitidum was extracted in the 70 ℃ water bath for 80 min with 6 times volume of pH 2.0 buffer for the first time,then for 60 min with 6 times volume of pH 7.5 buffer for the second time,then for 50 min with 6 times volume of pH 8.3 buffer for the last time.Conclusion The process is efficient,energy and timesaving.It establishes an experimental basis for industrial production.

  • 药物制剂与药品质量控制
    FANG Dan-dan;DENG Mao-fang;SHI Jing
    2013, 32(10): 1340-1343. https://doi.org/10.3870/yydb.2013.10.027
    Objective To optimize the preparation technology and the formula of megestrol acetate dispersible tablets and evaluate the quality.Methods Orthogonal L9(34) test was designed to optimize the prescription.UV spectrophotometry was used to detect dissolution of megestrol acetate dispersible tablets and HPLC method was established to determine the content of megestrol acetate and the related substances.Results The optimized prescription assembly was 53% of megestrol acetate,20% of lactose,1% of hydroxypropyl methyl cellulose,8% of microcrystalline cellulose,10% of sodium carboxy methyl starch,3% of colloidal silicon dioxide,1% of magnesium stearate.The test showed that disintegration time was less than 90 seconds,dispersible homogeneity was good,and dissolution rate at 15 min was more than 85%.Conclusion The optimal prescription is feasible,the preparation technology was simple and convenient.The dispersible tablets conform to the quality standard in the Chinese Pharmacopoeia 2010 edition of general preparations.
  • 药物制剂与药品质量控制
    JIN Peng-fei;XIA Lu-feng;ZOU Ding;WU Xue-jun;JIANG Wen-qing;HU Xin
    2013, 32(10): 1344-1347. https://doi.org/10.3870/yydb.2013.10.028
    Objective To establish a high performance liquid chromatography (HPLC) method for determination of dissolution of dronedarone hydrochloride tablets. Methods An Agilent ZORBAX Eclipse Plus C18 column (100 mm×4.6 mm,3.5 μm) was used for the separation,with 2 mmol.L-1 sodium dihydrogen phosphate (pH was adjusted to 7.0 with NaOH)methanolacetonitrile (20:30:50) as the mobile phase at the flow rate of 1.5 mL.min-1.The detection wavelength was 290 nm.Paddle method was used in the dissolution test with 900 mL of water as the medium at the rotation speed of 125 r.min-1,and the sampling time was 60 min. Results The excipients did not interfere with the analysis of dronedarone;the correlation coefficient (r) was 1.000 0;the lower limit of detection (LOD) was 0.19 mg.L-1 (as dronedarone);the precisions and 12hour stability were satisfactory with all RSD below 1%;the spiked recovery ranged from 101.2% to 102.1%.The dissolution tests indicated good repeatability from 3 batches of samples with RSD all below 7%,and the average dissolutions were all above 75%.Conclusion This method is accurate,fast and simple,and can be used for the determination of dissolution of dronedarone hydrochloride tablets.
  • 药物制剂与药品质量控制
    MA Gai-xia;GUO Peng-fei;HU Peng
    2013, 32(10): 1348-1352. https://doi.org/10.3870/yydb.2013.10.029
    Objective To optimize the extraction techniques for qing’e granules.Methods Extraction rate and contents of pinoresinol diglucoside,psoralen and isopsoralen were used as investigation indexes.The optimum extraction conditions were studied with the orthogonal test of L9(34). Results The optimum extraction conditions were A2B1C2D3,i.e.ten times volume of 70% alcohol was added each time,and heated for extraction for 2 h at each time for three times,and alcohol was recycled by decompressed concentration. Conclusion The extraction technique is rational and the extraction rate of active components is high,which provides a experiment basis for further preparation research and clinical application of qing’e granules.

  • 药物制剂与药品质量控制
    DENG Yong-qiang;NIE Chun-fa;SHI Ying;WANG Xiao-ni;SUN Yang-yang
    2013, 32(10): 1352-1355. https://doi.org/10.3870/yydb.2013.10.030
    Objective To establish a quality control method of qiguanyan pill.Methods TLC method was used to differentiate the ephedrine,codonopsis and citri grandis exocarpium.HPLC method was employed to determine naringin content.A C18 column (4.6 mm×150 mm,5 μm) was used with methanol and water (40:60) as a mobile phase.The flow rate was 1 mL.min-1,detection wavelength 283 nm. Results Samples and controls had the same color fluorescence spots at the same position.Spots were well isolated.Linear range was 0.195 2-1.952 0 μg (r=0.999 9,n=7).Average recovery was 100.82% (RSD=0.87%,n=6) .Conclusion The established method was sensitive,accurate,repeatable and specific for determination of naringin.It can be used for quality control of qiguanyan pill.