药物研究
YU Wei-fan
Objective To study the protective effect of total flavones of Herba verbenae (TFH) against carbon tetrachloride (CCl4)induced acute liver injury in mice.Methods Sixty mice were randomly divided into six groups: normal group,model group,positive control group,high,medium,and lowdose TFH group.The mice of normal and model groups were given normal saline,and mice of all TFH groups and positive control group were administered with TFH (50,100,200 mg.kg-1)and bifendate (200 mg.kg-1),respectively.All the mice were treated with TFH or bifendate for 10 consecutive days,once daily.One hour after the last administration of the drugs,each mouse was intraperitoneally injected with 0.15% CCl4,except the mice in the normal group.Twenty four hours after the last administration of the drugs,the activity of alanine aminotransferase (ALT),aspartate aminotransferase (AST),interleukin (IL)6,tumor necrosis factor (TNF)α,and interleukin (IL)8 in the serum,liver index,spleen index,thymus index,the expression of nuclear transcription factor (NF-κB) p65,the activities of superoxide dismutase (SOD),glutathione peroxidase (GSH-Px),catalase (CAT),and the contents of malondialdehyde (MDA),and nitric oxide (NO) in liver tissues were investigated.Results In highand mediumdose TFH groups,the serum activities of ALT and AST were significantly decreased (P<0.01).The liver index,spleen index,thymus index were significantly reduced (P<0.05 or P<0.01).The expression of NF-κB p65 was inhibited in the liver tissue,and the contents of IL-6,TNF-α,IL-8 were decreased in the serum (P<0.05 or P<0.01).The activities of SOD,GSH-Px and CAT were elevated in the liver tissue,and the content of MDA,NO were reduced (P<0.05 or P<0.01).Conclusion TFH can alleviate liver injury,protect liver cells,and reduce inflammatory exudation and oxidative injury.