药物研究
WU Weifeng;LV Bin;ZHANG Shuo;YU Leimin
ABSTRACTObjectiveTo set up the small intestinal mucosal damage model by shortterm administration of diclofenac at low dose. And to investigate the preventive effect of mica on NSAIDs induced small intestinal damage, providing the experimental foundation for preventing and controlling this damage. MethodsFortyeight rats were divided into 3 groups(control group, model group and mica group), and each group with 16 rats were separated into T1（acute stage）, T2（subacute stage） subgroups. Rats in the mica group were lavaged with mica(60 mg•kg1) 1 day beforehand,and then with both mica 60 mg•kg1 and diclofenac 7.5 mg•kg1, bid；rats in the control group were treated with 1 mL distilled water, bid； in the model group treated with diclofenac 7.5 mg•kg1, bid. The T1 and T2 group were killed in 1 d and 5 d later,respectively. ResultsA single administration of lowdose diclofenac induced multiple lesions in the small intestine, such as obviously erythema, erosion, ulcer and cystoid expansion. The lesions of the model T2 subgroup was more seriously than those in the T1 subgroup (P＜0.05). And the lesions in the model group was also more obviously than those in the control and mica group (both T1 and T2 , P＜0.05). Compared with the control group, the serum content of NO in the model T1 subgroup was significantly lower (P＜0.05), while that in T2 subgroup was higher (P＜0.05)； no difference was found among the mica group and model groups (each P＞0.05). ConclusionShort term administration of lowdose diclofenac elicites intestinal mucosal lesions and aggravates as time goes on. The NO content in serum presents a tendency with decrease at first but increase later. The mica has certain preventive effect on small intestinal lesions,which maybe not rely on the NO pathway.
