药物研究
GONG Weimin;ZH0U Zewei;SHEN Xiu;SHEN Hongsheng;WANG Jie
2009, 28(8): 996-999.
ObjectiveTo study the antitumor effect of TH in vitro and in vivo and identify a new highefficient and lowtoxic compound recipe.MethodsInhibition rate of growth and halfinhibitory concentrations (IC50) were assessed in human lung cancer cell A549, human esophageal carcinoma cell EC9706,human colon cancer cell LoVo, human hepatocellular carcinoma cell HepG2 and human breast cancer cells MCF7 by MTT method.The mice transplanted with hepatoma H22 were randomly divided into 5 groups: negative control, positive control (CDDP0.005 g•kg1), three doses of TH(0.030,0.060,0.090 g•kg1), which were treated intragavagely with 0.3 mL of solution since the next day, for consecutive 8 d.Other groups were treated with 0.2 mL every other day for total three times.Mice were sacrificed 24 h after the last drug administration the indexes of thymus, spleen and inhibitory rates were measured.ResultsIC50 of TH in A549,EC9706,LoVo,HepG2 and MCF7 were 7.15,5.41,2.49,2.18 and 1.37 μg•mL1,respectively.Tumor inhibitory rates of low, middle and high dose group of TH in mice with H22 were 52.8%,44.9% and 47.9%,which were significantly higher than those of the control group (P＜0.01).The indexes of spleen,thymus and liver in TH groups showed no remarkable changes compared with those in the control group, but were significantly different from those in the positive control(P＜0.01).ConclusionTH has strong antitumor activities by markedly inhibiting cell growth in vitro and suppressing tumorigenesis in vivo.
