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    药物研究
  • 药物研究
    LI Zhi-jian;SILAFU&#;Aibai;GULINAR&#;Dawuti;AN Hui-xia;XIAO Wei;ZHOU Fan
    2009, 28(7): 830-832.
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    To study the effect of extracts from Euphorbia humifusa on ergosterol biosynthesis in fungus. Methods The fungi were incubated for 7 d with different concentrations of Euphorbia humifusa extracts, and the content of ergosterol in which was detected by HPLC after saponification and extraction. Conclusion Euphorbia humifusa extracts inhibited the ergosterol biosynthesis(P<0.01) in a dosedependent manner. Conclusion Euphorbia humifusa extracts has remarkable antifungal activity by inhibiting the ergosterol biosynthesis in fungus.
  • 药物研究
    CHEN Qinhua;HE Jing;ZHANG Hong;LI Peng;SHI Xiaoya
    2009, 28(7): 840-842.
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    ABSTRACT Objective To develop a rapid method for determination of yohimbine in human plasma by nonaqueous capillary electrophoresis (NCAE). Methods The separation conditions of NCAE were 30 mmol&#8226;L1 ammonium acetate in methanolacetonitrile (4:1, V/V , added 1% glacial acetic acid)as running buffer, UV detection was set at 220 nm, separation voltage was 25 kV, column temperature 25 ℃ and pressure injection was 50 kPa×5 s. Internal calibration curve of peak area was selected to quantify. Conclusion The limits of detection was 0.01 μg&#8226;mL1. The concentration of yohimbine showed a linear plot in the range of 0.05485.48 μg&#8226;mL1 ( r= 0.999 1) . Interday and intraday precisions expressed as the relative standard deviation (RSD) for the method was 2.81%5.22% and 4.72%6.52%, respectively. Conclusion This method is rapid and accurate for the quantitative analysis of yohimbine in human plasma.
  • 药物研究
    BU Yuehua;HONG Bo;ZHAO Min;ZHAO Chunjie
    2009, 28(7): 846-848.
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    ABSTRACT Objective To evaluate the bioavailability and pharmacokinetics of two preparations of calcium folinate in healthy volunteers. Methods The randomized, crossedover study was conducted in 18 healthy volunteers. After a single dose of administration (containing 90 mg calcium folinate dispersed tablets), the plasma levels of medicines were determined by HPLC. The AUC0~12 of both forms were compared by variance analysis and twoway onesided t test. Conclusion The main pharmacokinetics parameters of test and reference preparation were as followed: tmax were (1.5±0.0) h and( 1.5±0.0) h, Cmax were (2 295.51±368.93) ng&#8226;mL1 and (2 139.53±189.67) ng&#8226;mL1, t1/2 were (2.19±0.16) h and (2.23±0.17) h, AUC012 were (6 202.09±229.90) ng&#8226;mL1&#8226;h and (6 185.32±191.47 ) ng&#8226;mL1&#8226;h, and AUC0∞ were (6 478.43±250.69 ) ng&#8226;mL1&#8226;h and (6 478.63±248.10 ) ng&#8226;mL1&#8226;h, respectively. Conclusion The Conclusion showed that the two formulations were bioequivalent. The relative bioavailability of calcium folinate dispersed tablets was (100.13±5.39)%.
  • 药物制剂
  • 药物制剂
    ZHAO Yan-ling;XIAO Xiao-he;SHAN Li-mei;ZHOU Xu
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    ABSTRACT Objective To optimize the extraction process of Rhizoma zingibers by central composite design/response surface methodology. Methods Multiple linear regression and binomial fitting models were performed with grinding size, volume of water added and refluxing time as the independent variables and extraction rate of 6-gingerol as dependent variable. Response surface methodology was used to optimize the extraction process and to predict the values. Results The optimum conditions of extraction process were determined as pulverizing the herbs into 30 meshes powder, adding 10-fold solvent and extracting for 2 times,10 hours once. The bias between observed and predicted values was -0.90% and the correlation coefficient of binomial fitting complex model was as high as 0.954 8. Conclusion Central composite design/response surface methodology is convenient and highly predictive for optimizing the extraction process of Rhizoma zingibers.
  • 药物制剂
    HUANG Fei-feng;BAO Jun;ZHOU Han-ming
    Objective To select the optimal formulation of valaciclovir bioadhesive microspheres and investigate its mucoadhesive property. Methods The optimum formulation was investigated by orthogonal design on the yield and entrapment efficiency. The content of valaciclovir in prepared bioadhesive microspheres was analyzed by HPLC and its mucoadhesive property was studied by detecting stagnation percentage. Results The best formulation of valaciclovir bioadhesive microspheres presented as: the administration of valaciclovir was 30%, the ratio of disperse phase to continuous phase was 0.70 and the amount of Span 80 used was 8.0 g. The stagnation percentage of valaciclovir bioadhesive microspheres in vitro and in vivo were (90.8±3.9)% and (66.5±12.7)%, respectively. Conclusion The formulation and preparation of valaciclovir bioadhesive microspheres were reasonable and reliable, which had a good adhesiveness both in vitro and in vivo.
  • 药品质量控制
  • 药品质量控制
    WANG Zhong;ZHANG Chang-gong;FU Qin-qin;WANG Wei;WANG Qiong;FANG Wei
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    Objective To establish a method for quantitative determination of linoleic acid(LA) in boxintong. Methods The optimal condition was determined by gas chromatography. The column was used as a highly polar (30 m×0.53 mm,1 μm) fused silica capillary column. The best initial oven temperature was held at 120 ℃ for 2 min and then increased at 10 ℃&#8226;min-1 to 180 ℃ held for 2 min, then increased at 2 ℃&#8226;min-1 to 184 ℃, at last increased at 1 ℃&#8226;min-1 to 190 ℃. The samples were derivatized by the method of acidcatalyzed methylations. Results The content of LA was 8.16%, RSD was 1.66% and its precision was under 3%.The recoveries were at ranges of 97%-103%. Conclusion The proposed method is convenient, accurate and reliable for determination of LA.

  • 药品质量控制
    MA Li;HUANG Liang-sheng;ZHANG Yu
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    Objective To establish a HPLC method for determination of pantoprazole in entericcoated micropill capsule. Methods Symetry C18 (4.6 mm×150 mm,5 μm)column was employed as a stationary,mixture of acetonitrile and phosphate buffer (70:30) as a mobile phase. The detection wavelength was 288 nm, the flow rate was 1.0 mL&#8226;min-1. Results The sample was well isolated under the condition. The linear relationship was good for pantoprazole at the range of 36-84 mg&#8226;L-1, the function was Y=13 130C+155 709 (r=0.999 7).The RSD of precision and recovery was less than 3% and the content of pantoprazole was 99.53%. Conclusion This method is accurate, convenient and fast, which is suitable for determination of pantoprazole in entericcoated micropill capsule.