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药物研究

Etoposide Induces Apoptosis of Gastric Adenocarcinoma Cells Through Activating BH3only Protein Noxa

XIE Qipeng;ZHANG Linjie;ZHANG Xudong;PAN Jingjing

2009, 28(3): 279-282. https://doi.org/10.3870/yydb.2009.03.002

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ABSTRACTObjectiveTo examine the role of Noxa, the BH3only protein of the Bcl2 family, in apoptosis of gastric adenocarcinoma cells induced by etoposid. To find a new target for clinic treatment.MethodsThe survival rate was detected by MTT. The levels of apoptosis were quantitated using Annexin V staing. The role of caspases in etoposide induced apoptosis was studied using ZVADfmk. The expression of Noxa and p53 at mRNA and protein levels were measured by semiquantitative RTPCR and Western blot analysis, respectively. ResultsEtoposide induced apoptosis of gastric adenocarcinoma cells in a dose and timedependent manner. It appeared that SGC7901 cells were more sensitive to the compound than BGC823 cells. Induction of apoptosis by etoposide in gastric adenocarcinoma is markedly inhibitedby the pancaspase inhibitor ZVADfmk. Treatment with etoposide resulted in a rapid upregulation of p53 and its transcriptional target Noxa at the mRNA level and protein level at 4 and 8 hours, respectively,after exposed to the compound in SGC7901 cells. The expression of p53 and Noxa at the mRNA and the protein levels remained unchanged in BGC823 cells exposed to compound. ConclusionsEtoposide induces caspasemediated apoptosis in gastric adenocarcinoma cells. Activation of p53 and consequent upregulation of the BH3only protein Noxa may play a critical role in the apoptosis.

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药物研究

A Study of Cefuroxime Sequential Treatment in Experiment Dogs

LU Xiaotong;TANG Yuenian;JIANG Yuelian;PENG Zhengru

2009, 28(3): 283-285. https://doi.org/10.3870/yydb.2009.03.003

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(1. ABSTRACTObjectiveTo observe the change of plasma cefuroxime level in experiment dogs. MethodsThe research was performed using a randomized and selfcross control method . Cefuroxime sodium 50 mg•kg1 was delivered through intravenous injection at first duration. At second duration, experiment dogs were intravenously injected cefuroxime sodium 50 mg•kg1，and followed by oral taken cefuroxime 50 mg•kg1 after 6 h. Plasma cefuroxime level was detected at each time point. ResultsBlood drug concentration of cefuroxime in two groups has significant difference .ConclusionIt is an effective treatment that cefuroxime was delivered through a sequential method.

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药物研究

Pharmacokinetics and Bioequivalance of Two Preparations of Ligustrazine Phosphate in Healthy Volunteers

HUANG Yizhi;CHEN Chao

2009, 28(3): 285-288. https://doi.org/10.3870/yydb.2009.03.004

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ABSTRACTObjectiveTo study the pharmacokinetics and bioequivalance of ligustrazine phosphate capsule and dropping pill in healthy volunteers．MethodsIn a randomized crossover study，20 healthy volunteers were assigned to receive a single oral dose of 200 mg ligustrazine phosphate capsule and dropping pill ．Ligustrazine phosphate was processed by Waters HLB solidphase extractor and its serum concentration was determined by an HPLCUV method . The main pharmacokinetic parameters and the bioequiavailability were evaluated. ResultsThe pharmacokinetics process was in accordance with the open twocompartment model. The main pharmacokinetic parameters for two preparation of ligustrazine phosphate were as follows：t1/2(ka) were（0.48±0.09） and （0.21±0.08）h, respectively；t1/2(α) were（0.70±0.12）and（0.68±0.11） h, respectively；t1/2(β) were （3.97±0.20） and（3.62±0.23） h, respectively；tmax were（0.83±0.21） and（0.80±0.25） h, respectively；Cmax were（1.33±0.20） and（1.35±0.23） μg•mL1 respectively；AUC0～10 were （3.22±0.89） and （3.34±0.91） μg•h•mL1, respectively； AUC0～∞were (3.52±0.84) and（3.66±0.92）μg•h•mL1, respectively. AUC0～10，AUC0～∞and Cmax were similar between the two preparation.The relative bioavailability of ligustrazine phosphate capsule was (96.17±13)% according to AUC0～∞．ConclusionThe two preparations of ligustrazine phosphate are bioequivalent．

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药物研究

A Determination on Release in vitro and Bioavailability of Sinomenine Hydrochloride Sustainedreleased Capsules in Dogs

ZHAO Tie;LI Yuan;ZHAO Jiewen;ZHANG Wei;JIAN Lingyan;HE Zhonggui

2009, 28(3): 289-291. https://doi.org/10.3870/yydb.2009.03.005

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（1. ABSTRACT ObjectiveTo study the pharmacokinetics and relative bioavailability of sinomenine hydrochloride sustainedreleased capsules in dogs. MethodsSingle oral dose of 120 mg sinomenine hydrochloride sustainedreleased capsules and zhengqing fengtongning sustainedreleased tables were given to 6 dogs in an open randomized crossover way to study the pharmacokinetics parameter and relative bioavailability. Sinomenine hydrochloride concentration in blood was determined by an HPLC method. ResultsThe timeconcentration curve of sinomenine hydrochloride sustainedreleased capsules was similar to that of zhengqing fengtongning sustainedreleased tables ,and their AUC0t was 13 503.40 and 13 057.73 ng•h•mL1; Cmax was 893.12 and 1 334.45 ng•mL1; t1/2 was 20.72 and 8.57 h; tmax was 9.83 and 2.67 h, respectively. The result showed that there was no significant difference between sinomenine hydrochlorid sustainedreleased capsules and zhengqing fengtongning sustainedreleased tablets. ConclusionThe two formulations of sinomenine hydrochloride sustainedreleased capsules and zhengqing fengtongning sustainedreleased tables were bioequivalent, although the fore has a longer t1/2 and more postponed tmax.

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药物研究

Protetive Effect of Penotoxifylline on Rats with Renal Ischemiareperfusion Injury

YANG Xianghong;CHEN Jianghua

2009, 28(3): 292-295. https://doi.org/10.3870/yydb.2009.03.006

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ABSTRACT ObjectiveTo investigate the effect of penotoxifylline(PTX) on the expression of ICAM1 in renal ischemiareperfusion injury ,and to evaluate the protective mechanism of PTX on renal. MethodsThe rat model of renal IRI was established with occlusion of left and right kidney pedicle for 45 minutes. 75 SD rats were randomly divided into three groups: the sham operated controls, IRI group and PTX treated group. The serum creatinine (sCr) values and the levels of ICAM1 and TNFα in kidney tissue were measured before operation and 0, 1, 4, 24 hours after reperfusion. ResultsThe renal tissue histophathology was ameliorated in PTXtreated group. The valuels of sCr , the scores of renal tubules, the levels of ICAM1 and TNFα in kidney tissue in PTXtreated group were significantly lower than those in IRI group . ConclusionPTX pretreatment can attenuate kidney IRI by reducing expression of ICAM1 and TNFα in kidney tissue.

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呼吸系统疾病用药专栏

Antitussive , Expectorant and Antiasthmatic Effects of Keerkang Oral Liquid in Experimental Animals

SUN Zhanpeng;LI Mengquan;WANG Yuanyi;LI Houzhong;JIN Xiudong

2009, 28(3): 297-298. https://doi.org/10.3870/yydb.2009.03.008

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ABSTRACTObjectiveTo observe the antitussive, expectorant and antasthmatic effects of keerkang oral liquid in experimental animals. MethodsMice or guinea pigs were divided into three groups: ① control group which was intragastrically administred with saline, ② tested group which was intragastrically administred with different concentration of keerkang oral liquid, ③ positive group which was intragastrically administred with acute bronchitis syrup, ammonium chloride and fufang anchajian mahuang tables for the tests of antitussive, expectorant and antiasthamtic roles respectively. The cough latency of mice stimulated by ammonia and the number of cough in 3 min were detected to evaluate antitussive role; the phenol red excretin text to evaluate the expectorant effect; and the asthma latency of guinea pigs induced by the mixed liquor of acetylcholine and histamine to evaluate antiasthmatic effect. ResultsKeerkang oral liquid signifficantely prolonged the latency of cough; reduced the number of cough in 3 min; increased phenol red excretion; prolonged the latency of asthma. ConclusionKeerkang oral liquid has antitussive, expectorant and antiasthmatic effects in the experimental animals.

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呼吸系统疾病用药专栏

The Clinical Study of Chinese Herbal Qingyan Vaporous Atomization Treating Chronic Pharyngitis

XU Yu;HUA Qingquan;TAO Zezhang;CHEN Guifang

2009, 28(3): 299-301. https://doi.org/10.3870/yydb.2009.03.009

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ABSTRACT ObjectiveTo investigate the therapeutic effects of Chinese herbal qingyan vaporous atomization on chronic pharyngitis. Methods 97 patients with chronic pharyngitis were divided into two groups: research group treated with qingyian ultrasonic steam inhalation and the other group treated with traditional western medicine steam inhalation. Changes in the main symptoms and signs were comparatively observed before and after the therapy in 2 groups. Then the pharynx mucosal tissues biopsies were detected using light and electron microscope before and after therapy. ResultsThe total effective rates for chronic pharyngitis were 92.86% and 75.60% respectively in Chinese and western medicine ultrasonic steam inhalation groups (P＜0.05). The histological observation revealed that after Chinese herbal qingyan therapy, the number of inflammation infiltration cells deceased, the contour of nuclear membrane became clear, and the secretion of glandular organ reduced. ConclusionThe Chinese herbal qingyan ultrasonic steam inhalation therapy is an effective therapeutics for the treatment of chronic pharyngitis through significantly inhibiting the inflammation condition of local mucosal tissue .

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呼吸系统疾病用药专栏

Ulinastatin for Acute Lung Injury and Acute Respiratory Distress Syndrome :A Systematic Review

WU Jian;LI Peng

2009, 28(3): 302-304. https://doi.org/10.3870/yydb.2009.03.010

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(1. ABSTRACTObjectiveTo assess the effectiveness and safety of ulinastatin in patients with acute lung injury or acute respiratory distress syndrome. MethodsA systematic review of randomized controlled trials (RCT) of ulinastatin for acute lung injury and acute respiratory distress syndrome performed. Trials were identified by searching CBMWeb, CMCC and CNKI. All RCTs comparing ulinastatin with basic treatment were included. Statistical analysis were performed by using RevMan 4.2.10 supported by Collaborative Review Group. ResultsNine trials involving 536 patients were included. Most trials were of poor quality. Metaanalysis of 5 RCTs showed that the mortality of ulinastatin plus basic treatment group (A group) was 20.55% (30/146), and was 46.85% (67/143) in basic treatment group (B group). A statistic significant difference was found between the two groups (OR 0.24, 95%CI 0.14 to 0.42, P＜0.01). Metaanalysis of 8 RCTs showed that oxygenation index of A group has a more significant improvement than that of B group (SMD 3.04, 95%CI 1.66 to 4.43, P＜0.01). The adverse events of ulinastatin appeared to be infrequence. ConclusionUlinastatin appears to be a safe and effective treatment,although there is no enough evidence to support at present as the published trials was poor quality. Trials with enough sample size and scientifically sound methodology are required.

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药物与临床

Effect of Arginine on Serum Tumor Necrosis Factor Receptor 1(sTNFR1) and Tlymphocytes rDNA Transcription Activity in Peripheral Blood of Patients with Pancreatic Cancer after Intraarterial Chemotherapy

CHEN Guanglan;WANG Wangyue;LIU Xiaofen;ZHU Meiping;LI Hongguang

2009, 28(3): 310-312.

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ABSTRACTObjectiveTo study the effects and clinical significance of arginine on serum soluble tumor necrosis factor receptor 1 (sTNFR1) and Tlymphocytes rDNA transcription activity in peripheral blood of patients with pancreatic cancer after intraarterial chemotherapy MethodsThe level of sTNFR1 and Tlymphocytes rDNA transcription activity in peripheral blood of 50 patients with pancreatic cancer before and after intraarterial chemotherapy were measured by ELISA and a cell image analysis of AgNORs respectively, and compared with that of health controls. ResultsBefore intraarterial chemotherapy ,the level of sTNFR1 was higher while Tlymphocytes rDNA transcription activity of PBL was lower than that of health controls(P＜0.01).After intraarterial chemotherapy, sTNFR1 declined while Tlymphocytes rDNA transcription activity elevated (P＜0.05).The effects in the group combined with immune nutrition therapy (arginine) was more obvious than that in the group without (P＜0.05). ConclusionsTNFR1 and Tlymphocytes rDNA transcription activity is helpful to evaluate the patients immune state . The intraarterial chemotherapy combined with suitable immune nutrition therapy can improve immune function of the patients with pancreatic cancer.

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药物与临床

Effects of Rhubarb and Medicinal Charcoal Coloclysis on Patients with Chronic Renal Failure

LIU Hongyan;WANG Rui

2009, 28(3): 313-315.

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ABSTRACT ObjectiveTo investigate the effect of rhubarb and medicinal charcoal powder coloclysis on patients with chronic renal failure(CRF). MethodsForty patients with CRF were divided into two groups: group A as a control was treated with rhubarb and orther traditional medicine coloclysis, group B was treated with both rhubarb and medicinal charcoal powder coloclysis. The levels of hemoglobin (Hb), serum total protein (TP), albumin (ALB), blood urea nitrogen(BUN),serum creatintine(Scr), serum uric acid (UA),CO2CP and Na+ were measured before and after treatment. ResultsThe levels of BUN, Scr and UA were decreased after treatment in group A and B (P＜0.01). The levels of Hb, TP and ALB were increased after treatment (P＜0.01) in two groups. A significant CO2CP improvement,rather than Na+, was observed after treatment in group B (P＜0.01). The degree of Scr and UA improvement after treatment in group B was more remarkable,compared with group A(P＜0.05). eGFR in group B was higher compared to that in group A after treatment(P＜0.05) . ConclusionCombination of rhubarb and medicinal charcoal powder coloclysis is an effective method for CRF patients.

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药物与临床

The Choice of the Immunosuppressant for the Patients with Malignant Tumors after Kidney Transplantation

WANG Haihao;ZHANG Weijie;CHEN Zhishui;MEI Qi;MA Ke

2009, 28(3): 315-317.

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（1. ABSTRACT ObjectiveTo evaluate the efficacy and safety of the immunosuppressant in 10 postrenal transplantation recipients with malignant tumors. MethodsA sirolimus（SRL）based immunosuppressive treatment was performed for 10 cases who have malignant tumors after kidney transplantation. During the followup period, the recurrence and diffusion of the tumor, the renal function and rejection reaction were monitored. ResultsAll these cases have been followed up for at least 1 year. 9 cases had no recurrence and diffusion. 1 case died due to the tumor diffusion 7 months later from the drug conversion. 1 case suffered once acute rejection reaction 2 months later from the drug conversion. This acute rejection was inhibited by emergency dose MP. ConclusionAs a new immunosuppressant, not only can SRL prevent the generation of AR, but also inhibit generation and development of malignant tumors in kidney transplantation recipients.

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药物与临床

Application of LalanylLglutamine(LALG) Intensified Parenteral Nutrition Support in Critically Ill Surgical Patients

ZHANG Guoyong;WU Chengjun;HUANG Limei

2009, 28(3): 318-320. https://doi.org/10.3870/yydb.2009.03.017

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ABSTRACTObjectiveTo evaluate the effects of LalanylLglutamine(LALG) intensified parenteral nutrition support in critically ill surgical patients. Methods36 patients were randomizedly divided into two groups:control group(n=18) received only parenteral nutrition, intensified treatment group （n=18） received parenteral nutrition and LALG .Immune functions and patient tolerance were determined at pretreatment and 4th,7th,14th days after treatment. Results7 day’s treatment,the patients in two groups all turned from negative nitrogen balance to positive nitrogen balance.The nutrition and immune functions parameters increased significantly in both groups especially in LALG group.Blood glucose and total bilirubin increased obviously in control group rather than in LALG group. ConclusionLALG intensified parenteral nutrition has better effects on improvement of the nutrition and immune functions with a better tolerance

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药物与临床

A Comparison between Venlafaxine XR and Chlorimipramine in the Treatment of Somatization Disorder

QIAN Jianjun;YAN Weiliang;XU Baochang

2009, 28(3): 321-322. https://doi.org/10.3870/yydb.2009.03.018

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ABSTRACT ObjectiveTo compare the therapeutic and side effects between venlafaxine XR and chlorimipramine in the treatment of somatization disorder.MethodsOne hundred and thrity patients with somatization disorder were randomized into two groups, which were treated with venlafaxine XR or chlorimipramine respectively for 8 weeks. The symptomatic changes were evaluated using the total score of somatization, depression and anxiety as described in Symptom Checklist 90(SCL90) and Clinical Global Impressionseverity of illness( CGISI).The therapeutic effect was assessed using various subtraction rates, and drugrelated adverse reactions were evaluated using the Treatment Emergent Symptom Scale (TESS). ResultsThe recovery rate was 61.8% in the venlafaxine XR treatment group and 44.4% in the chlorimipramine treatment group, and the total effective rate was 89.1% and 75.9%, respectively. The difference in the efficacy between two treatments was significant (P＜0.05).TESS tests revealed that there were significant differences between two groups at various time points (P＜0.05). ConclusionVenlafaxine XR is effective in the treatment of somatization disorders, although has few adverse reactions.

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药物制剂

Preparation of Chloromycetin Prednisone Liniment by Ultrasonic

CHANG Mingquan;CHEN Fang;LV Ming;CHENG Xiaoli;HAO Xincai

2009, 28(3): 347-349. https://doi.org/10.3870/yydb.2009.03.032

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（1. ABSTRACTObjectiveTo study the preparation and establish a method for the chloromycetin prednisone liniment physical stability. MethodsThe optimal ultrasonic technology was used to produce chloromycetin prednisone liniment and the components of the agent was detected by HPLC. ResultsThe product was stable, with saved 30% ethanoland shortened preparation time . A good linear relation between choramphenicol and prednisone was observed within 0.30～1.50 mg•mL-1and 0.02～0.10 mg•mL－1 .The average reclaim rate of chloromycetin was 100.65%;and prednisone was 101.71%,respectively. ConclusionThe ultrasonic preparation techniqe is stable and accurate to yield chloromycetin prednisone liniment .

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药学进展

Identification of Compound Paracetamol and Amantadine Hydrochloride Tablets by Nearinfrared Diffuse Reflectance Spectroscopy

JIANG Yan;SHEN Jiate;JIANG Hong

2009, 28(3): 349-351.

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ABSTRACTObjectiveTo identify compound paracetamol and amantadine hydrochloride tablets by nearinfrared diffuse reflectance spectroscopy. MethodsNIR spectra of 37 samples from different batches of 18 factories was collected to form the calibration set to build the indentification model，and the other spectra of 23 samples from different batches of 10 factories including 6 batches false drugs were used to validate the performance of the model. ResultsThe results show that the model can distinguish all samples from 10 different factories actually. ConclusionThe method is convenient,fast and thus， can be used to detect the false drugs on the spot checkup.

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药物制剂

A Study for Preparation and Transdermal Test in vitro of Gegenqinlian Cataplasm

GUO Weiying;WANG Yu

2009, 28(3): 351-353. https://doi.org/10.3870/yydb.2009.03.034

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ABSTRACTObjectiveTo study the preparation of gegenqinlian cataplasm and its transdermal characteristics in vitro．Methods Gegenqinlian cataplasm was prepared using macromolecular water soluble materials．The level of Puerarin was determined by HPLC method. Its transdermal absorption properties were studied by Franz diffusion cell. The enhanced effects of azone for puerarin in gegenqinlian cataplasma were investigated. ResultsGegenqinlian cataplasm has favorable ductibility with a glazed surface. Puerarin could permeate through rat skin slowly；The permeation rates and the cumulative permeation amount of puerarin was improved markedly by azone . ConclusionThe gegenqinlian cataplasm is a new transdermal therapeutic system with sustained release property．

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药品质量控制

A Study of the Quality Standards of Medicinal Semen Alpiniae Katsumadai

JIN Hong;ZHAO Wenying;GONG Yanling

2009, 28(3): 354-356. https://doi.org/10.3870/yydb.2009.03.035

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ABSTRACTObjectiveTo build up quality standards for medicinal semen alpiniae katsumadai.MethodsThe contents of moisture, total ash, watersoluble extracts , 70% alcoholsoluble extracts, alpinetin, volatile oil of 10 batches of medicinal semen alpiniae katsumadai from different areas were determined. On the basis of these examinations, the quality standards were build up. ResultsThe average values of the indices of the semen alpiniae katsumadai were: moisture 12.43%, total ashes 4.10%, watersoluble extracts in hot water 2.60%, 70% alcoholsoluble extractive 2.47%, alpinetin 0.23%, volatile oil 1.38%, respectively. Conclusion It is suggested that the quality standards of medicinal semen alpiniae katsumadai could be tentatively arranged as follows: moisture 10.00%～15.00%, total ashes not more than 4.90%, watersoluble extracts in hot water not less than 2.10% , 70% alcoholsoluble extractive not less than 2.20%, alpinetin not less than 0.19%, volatile oil not less than 1.25%.

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药品质量控制

A Comparation on the Content of Sodium Taurocholate in Shedan Chuanbei Capsules from Different Manufacturers

WU Bingbing

2009, 28(3): 356-358. https://doi.org/10.3870/yydb.2009.03.036

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ABSTRACTObjectiveTo compare the content of sodium taurocholate in shedan chuanbei capsules from different manufacturers. MethodsThe content of sodium taurocholate was determined by HPLC. ResultsThe content of sodium taurocholate was significantly different in shedan chuanbei capsules made by five pharmaceutical manufacturers. ConclusionHPLC method to detect the content of sodium taurocholate was appropriate to control the quality of shedan chuanbei capsule.

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药品质量控制

Determination of the Content of Glucosamine Preparations by Derivatizationgas Chromatography Method

LI Jun;WANG Yingying

2009, 28(3): 359-362. https://doi.org/10.3870/yydb.2009.03.037

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ABSTRACTObjectiveTo establish derivatizationgas chromatography method for determination of glucosamine hydrochloride tablet and glucosamine sulfate sodium chloride capsule. MethodsGlucosamine reacts with hydroxylamine hydrochloride, and then acetic anhydride was added for acetylating reaction. The product was extracted with chloroform and washed with water. Water phase was left and organic phase was injected to GC for analysis after dehydration with anhydrous sodium sulfate. The gas chromatography is equipped with a flameionization detector and a HP5 column(30 m×0.32 mm, 0.25 μm). The injection and detector temperatures were 250 and 300 ℃, respectively. The carrier gas was nitrogen at a rate of 1.2 mL per minute and a split ratio of 1：1. The column temperature was maintained at 60 ℃ for 1 minute, then raised at a rate of 14 ℃ per minute to 260 ℃, and maintained at 260 ℃ for 5 minutes. The injection volume was 1 μL. Internal standard method was used for assay of glucosamine with mannose as internal standard. ResultsThe optimized condition was as followed: 4.0 mL of 100 mg•mL1 hydroxylamine hydrochloride solution was added into 4.0 mg•mL1 of glucosamine, and the reaction was carried out at 80 ℃ of water bath for 5 minutes. The time of acetylating reaction was 10 minutes. Under the above chromatographic conditions, glucosamine was separated completely from mannose. The linear range of glucosamine was over 2.0~6.0 mg•mL1, and the average recoveries were 101.3%,101.1%. ConclusionThis method is specificand accurate to determine glucosamine and its preparation.

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