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    药物研究
  • 药物研究
    ZHANG Jing;LIU Xueying;LIU Linna;GAO Lei;LI Wei;WANG Qingwei
    2009, 28(12): 1527-1529. https://doi.org/10.3870/yydb.2009.12.001
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    (1. ABSTRACTObjectiveTo study kinetics of percutaneous absorption of essential oils from Angelica sinensis (Oliv.)Diels in rabbit skin.MethodsThe Franz diffusion cell was used and the level of ligustilide was determined by HPLC.The cumulative permeation amount per unit area and rate constants of ligustilide through rabbit skin were calculated.ResultsThe kenetics of different concentrations of essential oils were all consistent with Higuchi equations.ConclusionThe essential oil from Angelica sinensis (Oliv.)Diels has a good percutaneous permeability.
  • 药物研究
    WANG Jianzhi;WANG Changhong;ZHOU Fubo;CUI Xingang;LI Rong;MI Yanjun
    2009, 28(12): 1530-1532. https://doi.org/10.3870/yydb.2009.12.002
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    (1. ABSTRACTObjectiveTo study the influence of Curcuma longa on the exhausted swimming time and biochemical index of blood and organs of mice, probing the antisports fatigue effects of which and possible mechanism for its application development in the field of sports.MethodsCurcuma longa were given intragastrically to mice at different dose for 30 days,then continuously loadedswimming test was carried out. Results It was showed that Curcuma longa obviously prolonged the loadedswimming duration of mice, lowered blood urea nitrogen, decreased the consumption of liver glycogen, cut down blood post exercise lactic acid and increased the activity of lactic dehydrogenase. ConclusionCurcuma longa may have a significant effect on the adaptation to heavy exercise and prevent or eliminate fatigue post exercise.
  • 药物研究
    OUYANG Lu;WANG Xuanbin;GUO Hongli;CAO Mei;XIONG Xianming
    2009, 28(12): 1533-1535. https://doi.org/10.3870/yydb.2009.12.003
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    ABSTRACTObjectiveTo study effects of bushenhuayu extracts on bone density and morphology in ovariescastrated rats. Methods48 healthy SPF female SD rats were divided into blank group, model control group, nilestriol group as positive control;and bushenhuayu groups with high, middle and lowdose of treatent,respectively. Bone density and morphology was detected. ResultsCompared with models,high and middledose of bushenhuayu significantly increased the bone density(P<0.05) and amount of osteoblast(P<0.05) , while lowered osteoclast(P<0.05). The morphology data showed that the width of trabecularism was elevated, the amount was raised, structure was improved and gap shrinked in bushenhuayu treated groups as well. ConclusionExtract of bushenhuayu has a potential therapeutic effect on the high bone turnover osteoporosis caused by ovariescastrating, which is valuable for clinic application.
  • 药物研究
    ZHOU Dan;SHEN Yanchun;WANG Fang;CHEN Qiongxia;LI Yan;ZHANG Lei
    2009, 28(12): 1535-1539. https://doi.org/10.3870/yydb.2009.12.004
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    (1. ABSTRACTObjectiveTo investigate effects of melatonin on morphological changes and renal function in rat with 5/6 nephrectomy.MethodsThe SD rats (250-300 g) were assigned into three groups:sham operated, 5/6 subtotal nephrectomy(STN), and STN rats given with melatonin(5 mg•kg1•d1) groups.The levels of renal function and urinary protein, the rate of renal weight and body weight and changes of renal pathology under light and electron microscope were observed at eight different time periods of total 280 d.ResultsThe levels of plasma creatinine and blood urea nitrogen were significantly elevated at the 1st week and developed into renal failure in the 40th week of STN group.And glomerulis, slight proliferation of mesangial cells and slight dilation of renal tubule were found in STN group.The melatonin partly ameliorated renal function, predominantly reduced urinary protein excretion and retarded the appearance of renal failure.ConclusionThe 5/6 nephrectomy on rats is an ideal model of chronic renal failure caused by glomerular sclerosis and renal interstitial fibrosis.Melatonin can protect renal function and morphological change in the remaining kidney.
  • 药物研究
    LU Xinjian;ZHANG Jian;CHU Jianshe;ZHAI Lei
    2009, 28(12): 1540-1542. https://doi.org/10.3870/yydb.2009.12.005
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    ABSTRACTObjectiveTo investigate the effect of ginkgo biloba tablets on activiy of cytochrome P4501A2(CYP1A2)and Nacetyltransferase 2 (NAT2) in human and to forecast the drugdrug interaction and instruct clinic rational administration of it. MethodsCaffeine was used as a metabolic probe for CYP1A2、NAT2,before and after administration of ginkgo biloba tablets.Five major metabolites of caffeine in the urine of 30 volunteers were determined by RPHPLC method and then evaluated to detect the activity change of CYP1A2 and NAT2 by the ratio of metabolites. Results It was found that the average activity of CYP1A2、NAT2 were 2.976±1.428, 0.447±0.172 and 3.021±1.318,0.391±0.147 before treatment and 28 days after treatment, respectively.There was no statistical significance on CYP1A2,but on NAT2. ConclusionGinkgo biloba tablets has no influence on CYP1A2 activity, but the NAT2. They may not modify the efficacy of drugs which metabolized by CYP1A2,but can impact the effects of drugs which metabolized by NAT2.
  • 药物研究
    TAN Fang;PENG Yan;LI Ruisheng;ZHANG Li;GONG Peili
    2009, 28(12): 1543-1545. https://doi.org/10.3870/yydb.2009.12.006
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    (1. ABSTRACTObjectiveTo establish a method for determining concentration of terbinafine hydrochloride in human plasma by HPLC. MethodsLilopristone was chosen as the internal standard.Chloroform was used for extraction. Acclaim C18 column(250 mm×4.6 mm,5 μm)was used and the mobile phase was composed of methanol acetonitrile0.01 mol•L-1 potassium dihydrogen phosphate buffer (pH 3.9) (5:45:50),eluted at a flow rate of 1.0 mL •min1. UV detection wavelength was set at 224 nmResultsA good linear calibration curves was obtained at the range of 0.04~2.40 mg•L-1.The regression equation was C=0.003 2 Y+0.051 3,r=0.998 6 (n=7) calculated by least square method. The detection limit was 0.04 mg•L-1and the recovery was 99.2%~102.5% with intraday RSD less than 12% and interday RSD less than 8%. ConclusionThis method is simple, sensitive, accurate and reproducible for concentration determination of terbinafine hydrochloride in plasma.
  • 药物研究
    LEI Qi;ZHOU Su;GAN Qiang
    2009, 28(12): 1551-1553.
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    (1. ABSTRACTObjectiveTo determine inhibition of the proliferation of human ovarian cancer cells COC1 by ultrasonic irradiation and paclitaxelcarrying microbubbles. MethodsThe cells were randomly divided into six groups: control group,blank microbubbles plus ultrasound,paclitaxelcarrying microbubbles,paclitaxel,paclitaxel plus ultrasound,and paclitaxelcarrying microbubbles plus ultrasound. The inhibitory effect of the cell growth was observed by the MTT assay,cell morphology and apoptosis rate were detected. ResultsThe growth of COC1 cells were significantly suppressed by paclitaxelcarrying microbubbles plus ultrasound,the inhibitory rates of which were (43.60±2.64)%,(37.89±1.30)%,(28.95±2.89)% at 24,48,72 h,respectively. The most significant cell number reduction was found in the group of paclitaxelcarrying microbubbles plus ultrasound(P<0.05) and the decreased cell diopter,cell shrinkage and a lot of cell debris were observed as well. The apoptosis rate reached (52.67±9.29)% by PI/hoechst staining assay(P<0.05). Differences with other groups were significant. ConclusionLowintensity focused ultrasound could effectively enhance inhibition of proliferation of human ovarian cancer cells COC1 by paclitaxelcarrying microbubbles.
  • 肿瘤科用药专栏
  • 肿瘤科用药专栏
    ZHAO Weijuan;HUANG Tongrui;ZHANG Mei
    2009, 28(12): 1554-1555. https://doi.org/10.3870/yydb.2009.12.010
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    (1. ABSTRACTObjectiveTo study the perceutanous absorption of homoharringtonine ointment in vitro.MethodsRats skin was applied as absorption barrers to study influence of azone at different concentrations on homoharringtonine permeation.ResultsAzone enhanced skin permeation of homoharringtonine significantly.Compared with the blank ointment, the steady percutanous absorption rate of homoharringtonine ointment containing with 2% and 5% azone increased by 56.88% and 89.51%, respectively.ConclusionHomoharringtonine cream have a good percutanous permeability, and 5% azone is the best permeating accelerator.
  • 肿瘤科用药专栏
    LIU Jie;ZHUANG Qianyuan;ZHANG Weiyi
    2009, 28(12): 1556-1558.
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    ABSTRACTObjectiveTo explore the anticancer effects of mitomycin C (MMC) and grape seed procyanidin extract (GSPE) on human bladder cancer cell line BIU87. MethodsThe 3[4,5dimethylthiazol2yl]2,5diphenyltetrazolium(MTT)method was used to detect the inhibition rates of MMC and GSPE by single or combined usage, the combination index(CI)method of ChouTalalay was used to quantitatively analyze the combined effects. ResultsThe anticancer effects of MMC and GSPE were enhanced in a dosedependent manner, respectively. The mediaeffect concentrations were 15.422 and 195.865 μg•mL1, respectively. MMC was synergetic with GSPE (CI<1) at low concentrations (0%<fa<37%), while antagonistic (CI<1) at high concentrations (37%<fa<100%) when used combined with GSPE. The mediaeffect concentrations was 109.496 μg•mL1 (in which MMC was 8.111 μg•mL1, GSPE 101.385 μg•mL1). ConclusionThe combination effects of MMC and GSPE is synergetic at low concentrations, while antagonistic at high concentrations.
  • 肿瘤科用药专栏
    WU Yue;QIU Weidong
    2009, 28(12): 1558-1561. https://doi.org/10.3870/yydb.2009.12.012
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    ABSTRACTObjectiveTo study effects of parecoxib and sufentanl on postoperative analgesia and stress response of stomach cancer.MethodsSeventyfour ASA class ⅠorⅡ patients undergoing operation of stomach cancer were randomly divided into two groups:treatment group received intravenous parecoxib 40 mg immediately and 12 h after the end of operation(n=37) and control group received normal saline instead of parecoxib.The patients in both groups received PCA with sufentanl(2 μg•kg-1).The PCA pump was set up to deliver a bolus dose of sufentanl 0.1 μg•kg-1(lockout interval 10 min) and background infusion of 2 mL•h-1.The intensity of pain was measured by using a VAS (0-10,0=no pain,10=worst pain),and recorded at 4,8,12,24 h after operation.Venous blood samples were taken at 0,1,2,3,4 h during analgesia for detecting plasma interleukin6(IL6) 、tumor necrosis factorα(TNFα) 、cortisol(Cor) and blood glucose(Glu).The adverse effects were observed within 24h after operation.Results65 cases, 34 in treatment and 31 in control groups were accomplished.There were no significant differences in plasma IL6 level among T1-T4 and T0 in treatment group (P>0.05),while IL6 and TNFα levels were significantly lowered in treatment group than those in stomach control group at T2、T3、T4(P<0.05).There were obvious changes in plasma Cor and Glu level before and after analgesia, and which was significantly lower in treatment group than that in control group at T2、T3、T4(P<0.05).Compared with those in control group , VAS pain scores and the number of successfully dose delivery were significantly decreased in treatment group at T1、T2、T3、T4.There were no significant different adverse effects in the two groups was found in 24 h after operation. ConclusionParecoxib has a good postoperative analgesic and stress relieve effects, and reduces the suffentanl consumption.
  • 药物与临床
  • 药物与临床
    ZHU Rongfei;LIU Guanghui;HUA Qingquan;LIANG Jianping;MA Jian;HE Guangxiang;WANG Shenqing
    2009, 28(12): 1574-1577. https://doi.org/10.3870/yydb.2009.12.017
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    (1. ABSTRACTObjectiveTo evaluate the efficacy and safety of fexofenadine/pseudoephedrine sustained release capsule in the treatment of allergic rhinitis.Methods240 patients with allergic rhinitis were enrolled and divided into 2 groups equally in a randomized, doubleblind, doublesimulation, multicenter study.Patients in the test group were received fexofenadine/pseudoephedrine sustained release capsule, 2 capsules per day for 7~14 days; while in the control group was given with loratadine/pseudoephedrine tablet, 2 tablets per day for 7~14 days.The integrated efficacy, effective rate, adverse events and the outcome of laboratory examination were used to evaluate the efficacy and safety of these two drugs at the end of therapy.Results224 patients were eligible for efficacy analysis (113 in test group and 111 in control group).The effective rates were 92.92% in test group and 95.50% in control group with no significant difference between them(P>0.05).There were 45 adverse events developed in 29 patients mainly including dry mouth, dizziness, somnolence and hypodynamia(13 patients in test gruop and 16 patients in control group).The incidence of adverse events was no difference between the two groups (P>0.05).No severe adverse events occurred in both groups.ConclusionThe results of the study indicate that fexofenadine/pseudoephedrine sustained release capsule could control the symptoms of allergic rhinitis effectively and is welltolerated.
  • 药物与临床
    LV Guangxiu;LAI Wen;JIN Xiaoyi
    2009, 28(12): 1577-1579. https://doi.org/10.3870/yydb.2009.12.018
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    (1. ABSTRACTObjectiveTo investigate the influence of montelukast sodium combined bacillas calmate guerinpolysaccharide nucleinic acid (BCGPSN) upon serum LTs levels and curative effects for children with slight asthma.Methods239 eligible patients of slight asthma were selected and randomly divided into the treatment group (n=131) and control group (n=108),and they were treated with montelukast combined with BCGPSN (treatment group) once every other day and single mintelukast (control group), respectively for 72 d as 2 courses.The serum changs of LTs and clinical effect were compared between two groups.ResultsLTs level of patients in the treatment group was significantly lower than that in the control group.The total curative rates were 98.5% and 92.6%(P<0.05)for the treatment and the control, respectively.ConclusionThe combined treatment of montelukast sodium and BCGPSN has a good effect on patients of silight bronchial asthma by reducing the infammatory factors LTs .
  • 药物与临床
    2009, 28(12): 1581-1582. https://doi.org/10.3870/yydb.2009.12.020
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  • 药物制剂
  • 药物制剂
    ZHONG Ling;GUO Xiaoli;ZANG Zhihe
    2009, 28(12): 1600-1604. https://doi.org/10.3870/yydb.2009.12.030
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    ABSTRACTObjectiveTo study the prescription form for core of rutin osmotic pump. MethodsSinglefactor method was applied to study the types and doses of infiltration promoter with low and highmolecularweight polymers, adhesive agent, diluting agent, disintegrating agent and blocker, by using in vitro drug release, as well as the molding process as indicators. ResultsThe prescription form for rutin osmotic pump was composed as: sugar as lowmolecularweight infiltration promoter, medicinal starch as diluting agent, 10% PVPethanol as adhesives, stearate magnesium as lubricant; the common used PEO and HPMC retarded drug release in vitro; MCC was not good enough for adhesion. ConclusionThe conventional core accessories for osmotic pump with insoluble drugs as a singleroom and singlelayer model are not suitable for rutin osmotic pump, proper ones of which need to be further screened and optimized.
  • 药物制剂
    ZHENG Jiangping;HUANG Liangyong;DONG Yongcheng
    2009, 28(12): 1604-1606. https://doi.org/10.3870/yydb.2009.12.031
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    ABSTRACTObjectiveTo prepare the ophthalmic gel of tetracaine hydrochloric, and study its quality. MethodsTetracaine hydrochloric ophthalmic gel was prepared in an acidalkaline way by using Carbopol940 as matrix and then evaluated such as pH, identification, viscosity, osmotic pressure and contents. The release rate in vitro and eye irritation was measured as well. ResultsThe pH value of tetracaine hydrochloric ophthalmic gel was 6.8-7.8, viscosity was 3.6-5.6 Pa•s, and osmotic pressure was 225-275 mOsmol•kg1.The average recovery of tetracaine hydrochloric was 99.47%,RSD=0.21%(n=9);Both cumulative emissions and amount of gel solution of tetracaine shown zerolevel dynamic characteristics, and had a good linear relationship. None of the eye stimulus on rabbit was found. ConclusionThe preparing technique is simple and reliable and could be used in clinical practice.
  • 药物制剂
    CUI Chunying;LI Ximeng;LIU Hu;CUI Guohui
    2009, 28(12): 1606-1609. https://doi.org/10.3870/yydb.2009.12.032
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    (1. ABSTRACTObjectiveThe effect of nonionic wetting agents on the stability of the cation fat milk was examined,by using n3 unsaturated fatty acid enriched seal oil as oil phase. MethodsThe samples with homogeneous particle were prepared by magnetic mixing and high pressure emulsifying. The grain diameter, Zeta electric potential and pH were tested at different time interval.ResultsTMS at 0.10%~0.50% had great effects on Zeta electric potential, grain diameter and stability. It was found that the changes of the zeta potential and the size of emulsions were related to decreased pH of emulsions. ConclusionUnceasingly hydrolysis of fat to free fatty acids then ionizing RCOO influences the stability of sea oil cation fat milk.
  • 药品质量控制
  • 药品质量控制
    HUO Shixia;YAN Ming
    2009, 28(12): 1612-1614. https://doi.org/10.3870/yydb.2009.12.034
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    ABSTRACTObjectiveTo study impacts of semibionic extraction on isolating trace elements from psoraleae fructus. MethodspH values in three decoction and total decocting time for Cu、Zn、Mn、Fe extracting were investigated by observing the dissolution rate of Cu、Zn、Mn、Fe. ResultsThe pH value in the first decoction was more effective on dissolution of Cu、Zn、Mn;and the pH value in the third decoction obviously affected dissolution of Fe. The total decoction time made more influence on dissolution of Cu than Zn、Mn and Fe. ConclusionThe change of pH value impacts on dissolution of trace elements from psoraleae fructus.
  • 药品质量控制
    CHEN Qiang;MAO Chunqin;LU Tulin;DANG Xiaoping
    2009, 28(12): 1615-1617. https://doi.org/10.3870/yydb.2009.12.035
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    ABSTRACTObjectiveTo compare contents of steroidal glycosides and tenacissosideB in Marsdenia tenacissima from different habitats. MethodsContents of steroidal glycosides and tenacissosideB were determined by UV and HPLC, respectively, and the measurement was investigated systematically. ResultsThe average content of steroidal glycosides in Marsdenia tenacissima from Guangxi, Sichuan and Hubei were 5.7%,5.3%,4.8% and tenacissosideB were 0.22%,0.27%,0.20%, respectively .ConclusionMarsdenia tenacissima from Guangxi contains more steroidal glycosides, and that from Sichuan has more tenacissosideB.
  • 药品质量控制
    ZHU Xuesong;HUANG Xuejing;LIAO Jing;ZHENG Fang
    2009, 28(12): 1618-1620. https://doi.org/10.3870/yydb.2009.12.036
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    (1. ABSTRACTObjectiveTo establish a HPLC analysis method for contents determination of norfloxacin, metronidazole and beclomethasone propionate in compound norfloxacin eardrops. MethodsThe kromasil C18 column ( 4.6 mm×250 mm, 5 μm ) was used, the methanolacetonitrile0.025 mol•L-1 phosphoric acid solution(adjusted to pH 3.0 with triethylamine)water for injection was applied as mobile phase, and the sample was eluted in gradient. Results The linear ranges of norfloxacin, metronidazole and beclomethasone propionate were at 15.0~150.0 μg•mL-1(r=0.999 4), 15.1~151.0 μg•mL-1(r=0.999 3)and 2.6 ~ 26.0 μg•mL-1(r=0.999 4),respectively, and the average recovery rate (n=9)were 99.5%, 99.7% and 99.5% with RSD as 0.80%, 0.78% and 0.92%,respectively. ConclusionThe method is simple, rapid and sensitivity, which is suitable for the content determination of compound norfloxacin eardrops.
  • 药品质量控制
    WANG Zhanyi
    2009, 28(12): 1621-1623. https://doi.org/10.3870/yydb.2009.12.037
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    ABSTRACTObjective To establish the quality standard for wantaidan capsules. MethodsThe quality of Radix arnebiae, Flos sophorae, Honeysuckle and Salvia miltrorrhiza in the prescription was identified with thin layer chromatograph method(TLC).The contents of chlorogenic acid in lonicera japonica was determined by HPLC. ResultsChlorogenic acid showed good linear relationship when the content were in the range of 0.011 21~0.067 26 μg in the samples. The average recovery was 97.6%,RSD was 1.60%(n=6).ConclusionThe method is simple,stable,specificity and reproducible. So it can be used as quality standard for wantaidan capsules.