Archive

• 01 September 2008 Volume 27 Issue 9

    

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  药物研究
  麻醉科用药专栏
  药物与临床
  药物制剂
  药品质量控制
  

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药物研究
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  药物研究

  Tanshinone ⅡA Represses cfos、cmyc Expression of the Proliferating Vascular Smooth Muscle Cells Activated by Angiotensin Ⅱ

  PAN Yongjun;LI Xiaoyong;YANG Guangtian

  2008, 27(9): 1019-1021.

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  药物研究

  A Study on Antiinflammatory Effect of Danxiong Prescription

  WANG Lijuan;LI Xiaorong;LI Yuhang;XU Yanxia;XUE Ming

  2008, 27(9): 1022-1024.

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  ABSTRACTObjectiveTo study the antiinflammatory action of danxiong prescription(DXP). Methods40 Wistar rats with half and half in sex were divided randomly and equally into 5 groups: blank control group, prednisone (1 mg•kg-1)group and three DXP (330.0,66.0 and 13.0 mg•kg-1) groups. 0.9% NaCl, prednisone and three dosages of DXP were given intragastrically into rats of the 5 groups separately after the subcutaneous injection of 0.1 mL of egg albumin into the left sole of each rat. The thickness of swelling was measured by vernier at 0 ,0.5, 1, 2, 4 and 6 hours after injection . The above mentioned agents were given intragastrically to each rats, separately after the injection of 5 mL of 1.5% carboxymethyl cellulose saline solution into each rat.7.5 hour later, 0.1 mL of the solution was extracted out for counting the number of white cells. Each rat cut a 0.5 cm incision at left and right groins was embedded subcutaneously with a 20 mg cotton ball into the groins. Corresponding agents were given after the operation from day 1 to 7. On the 7th day, rats were sacrificed and cotton balls with granulomatous tissue were weighed. ResultsThe swelling of sole could be reduced by different dosages of DXP at different time intervals. The number of invaded white cells in the solution and the weights of granulomas could be reduced by high and middle dosages of DXP. ConclusionDXP had a credible antiinflammatory action.
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  药物研究

  Tissue Distribution of 10Hydroxycamptothecin in Mice after Intraperitoneal Injection

  ZHANG Jingjing;FANG Yun;WANG Xin;JIANG Junyi;YANG Yuanxun

  2008, 27(9): 1025-1028.

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  ABSTRACTObjectiveTo established an HPLCFLD method to determine tissue distribution of 10hydroxycamptothecin in mice after intraperitoneal injection. MethodsThe liquid chromatograph is equipped with a fluorescence detector and excitation wavelength and emission wavelength are 382 nm and 528 nm,respectively . The temperature of reversedphase column(250 mm×4.6 mm，5 μm) is maintained at 35 ℃. Camptothecin is used as an internal standard . The mobilephase is composed of CH3CNtriethylamine solution (0.1%,pH3.0,30：70,V/V). The flow rate is about 1.0 mL•min1.ResultsThe retention time of 10hydroxycamptothecin and camptothecin is 4.9 min and 10.1 min respectively .The minimum level of quantification is 0.25 ng• mL 1 of 10hydroxycamptothecin in the tissues(S/N>7).The recovery rate of low, middle and high concentration and interday precision of each tissue meet the requirement of detection . ConclusionHPLCFLD method might be suitable for determination of 10hydroxycamptothecin in tissues of mice.
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  药物研究

  Protective Effect of Danqi Yigan Granule on the Liver Chroically Injured by Carbon Tetrachloride in Rats

  CAI Dawei;LIU Shunliang;YIN Xiaofei;LI Dongmei;WANG Shujie;SHI Yuxiang;LI Hui

  2008, 27(9): 1028-1030.

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  ABSTRACTObjectiveTo validate the protective role of danqi yigan granule in antagonizing chronic fibrosis of rats liver caused by carbon tetrachloride(CCl4).MethodsHepatic fibrosis was induced via hypodermic injection of 40% CCl4(5 mL for the first time,3 mL per 3 days) for 9 weeks. The control group was treated by olive oil. Danqi yigan granule was administered at a dose of 2.5, 5 or 10 g•kg-1. ResultsObvious liver damage and chronic fibrosis were observed in the CCl4 group. The liver function were improved in three doses threatment of danqi yigan granule.Collagen in the liver tissue markedly declined at a dose of 5 or 10 g•kg-1,and serum hyaluronic acid also decreased obviously at a dose of 2.5 g•kg-1. Furthermore,the histopathological lesion was significantly allayed in ruangan granule groups.ConclusionDanqi yigan granule played an important role in preventing chronic fibrosis of rat liver.
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  药物研究

  Incread Secrection of Tumour Necrosis Factor Alpha and Interleukin 6 Induced by Lovastatin in Peripheral Blood Monocytes of Patients with Essential Hypertension

  GOU Lianping;QIN Jian;LV Zhan;FAN Qu ming;FENG Jing

  2008, 27(9): 1031-1033.

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  ABSTRACTObjectiveThe study was to detect the level of tumour necrosis factor alpha(TNFα) and interleukin 6（IL6）in patients with essential hypertension（EH）before and after treatment with lovastatin. Methods 31 patients with EH were enrolled into lovastatin group,and 22 healthy volunteers into control group. PBMC(peripheral blood monocytes) were cultured in vitro and stimulated by lipopolysaccharide(LPS) and lovastatin. The concentrations of TNFα and IL6 were compared in PBMC culture supernatant. TNFα and IL6 were measured by enzyme linked immunosorbent assay(ELISA)．Results① The level of TNFα and IL6 of PBMC was significantly increased in patients with EH compared with that in the control subjects (P＜0.05) .②After stimulated by LPS, TNFα and IL6 had been upregulated in both groups (P＜0.05),moreover TNFα and IL6 of the EH group were higher than that of control group (P＜0.05).③ TNFα and IL6 were dramatically inhibited by lovastatin in both groups (P＜0.05).ConclusionActivated PBMC with increased TNFα and IL6 secrection may be involved in the pathogenesis of essential hypertensive.It is possible that lovastatin therapy EH via this inflammatory pathway.

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  药物研究

  Inhibition of Lingqi Capsule to the Growth and Metastasis of Tumorbearing Mice of Lewis Lung Cancer and Its Effect on the Expression of Related Ngiogenesis Factor

  GANG Honglin;WANG Deming;YANG Yi;SU Yunming

  2008, 27(9): 1034-1037.

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  （1. ABSTRACTObjectiveTo investigate the effects of LQC to the growth, metastasis and related ngiogenesis factor of metastatic tumor through establishing tumorbearing mice of Lewis lung cancer. MethodsThe metastatic tumor model of tumorbearing mice was established with Lewis cell line .The inhibition rate of metastasis and metastatic tumor were detected after treatment with LQC. Immunohistochemistry was carried out to confirm the expression of bFGF and COX2 after treatment of tumorbearing mice with LQC. Spectrophotometer was used to detected the iNOS content of tumorbearing mice bloodserum after treatment with LQC. Results LQC could inhibite the tumor growth of tumorbearing mice of Lewis lung cancer. The inhibition rate were 35.64%，29.23%， 21.03%,respectively.LQC also showed markedly synergism and attenuation together with CYT. Meanwhile，LQC could inhibite the lung cancerometastasis and downregulate the expression of bFGF and COX2，could inhibit angiogenesis of lung cancer by reducing iNOS content of blood serum in mice. So LQC had the anti netustasis of lung cancer.ConclusionLQC has a strong inhibitory effect on both growth and metastasis of lung cancer, and the mechanism of that may be related with down regulation of COX2, VEGF and reducing iNOS content of blood serum.
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  药物研究

  Oridonin Upregulates PTEN Gene Expression and Induces Apoptosis of HepG2 Cells

  LI Boli;ZHU Min;WANG Chen;YAN Weihua;ZHOU Meiying;CHEN Baoguo;DAI Mei zhen;GAN Linhong

  2008, 27(9): 1038-1040.

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  ABSTRACTObjectiveTo investigate oridonin effect of apoptosis induction and the PTEN expression in HepG2 cell line. MethodsThe growth inhibition of cells induced by oridonin was analyzed by MTT assay and apoptosis was detected by flowcytomery. Agarose gel electrophoresis was performed to detect the DNA fragment. PTEN gene expression was determined by reverse transcription polymerase chain reaction (RTPCR). ResultsA significant growth inhibitory of HepG2 cells induced by oridonin in doseand timedependent manners were observed. After treatment with oridonin, apoptosis HepG2 cells were increased in a timedependent model. A typical apoptotic “DNA ladder” was observed in agarose electrophoresis images . Oridonin upregulated the expression of PTEN gene in a apoptosisdependent model. ConclusionOridonin suppresses proliferation of HepG2 cells in time or dosedependent manner and induces cell apoptosis by possibly upregulating the expression of PTEN gene.
麻醉科用药专栏
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  麻醉科用药专栏

  The Effect of Anesthesia with Sevoflurane on Brain Oxygen and Glucose Metabolism during Perioperative

  REN Qiusheng;WANG Junlu;YANG Tao;WU Jinze;ZHANG Shenggong;LAN Zhongyue

  2008, 27(9): 1045-1048.

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  (1. ABSTRACTObjectiveTo observe the changes of metabolism of cerebral oxygen, glucose and lactate during anesthesia with sevoflurane in neurosurgery procedure. MethodsThirty patients scheduled for neurosurgery were induced with propofol 2 mg•kg-1，sulfentany 0.5 μg•kg-1，vecurnium bromide 0.1 mg•kg-1, and maintained with sevoflurane inhalation and intermittent i.v boluses of sulfenany and vecurnium bromide. Blood samples were taken from both artery and jugular venous bulb to detect blood gas and glucose during preinducation, discission, operation completion and 10 min after extubation. The content difference of oxygen, glucose and lactate between arterial and jugular bulb (DajvO2,DajvGlu and DajvLac,respectively) was analyzed at each time phase. ResultsDajvO2 was significantly lower at before discission, closure completion, 10 min after extubation, compared with preinducation(P＜0.05). DajvGlu was moderately decreased at before discission, closure completion, 10 min after extubation, compared with preinduction. DajvLac was remarkedly increased at closure completion and 10 min after extubation than that at preinducation (P＜0.05 and P＜0.01, respectively). ConclusionSevoflurane anesthesia might decrease cerebral oxygen extraction, but not strongly impact on cerebral glucose extraction.
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  麻醉科用药专栏

  A Comparison among PCEA ，PCIA and PCSA on Stress in Total Hysterectomy Patients

  WU Shimin;ZHANG Xianwei

  2008, 27(9): 1049-1051.

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  ABSTRACTObjectiveTo compare analgesic efficacy and stress reaction of epidural , intravenous and subcutaneous patientcontrolled analgesia（PCA）of fentanyl mixed in postoperative total hysterectomy patients. Methods90 patients undergoing total hysterectomy in general anesthesia were divided into three groups with thirty cases in each group: epidural analgesia group (E), intravenous analgesia group (I) and subcutaneous analgesia group (S). The mixture of fentanyl 1 mg and lidocaine 150 mg was administered in group S and group I and the mixture of fentanyl 0.5 mg and bupivacaine 150 mg was used in group E. The visual analog scale（VAS）,times of PCA, patient’s satisfaction and incidence of side effects were recorded during the period of postoperative 48 hours. Blood samples were taken at 1 h before operation, and 4, 16, 48 h after operation to detect cortisol and βendorphin. ResultsThere were no significant differences in the VAS scores, demanded delivery and patient’s satisfaction in 3 groups（P＞0.05）. At 4 h after operation,the concentrations of cortisol and βendorphin in three groups were significantly higher than those at 1 h before operation(P＜0.05)；The concentrations at 16 h after operation in group S and group I were also significantly higher than those in group E (P＜0.05). At 48 h after operation, concentrations was no remarked differences in the among the three groups, and similar to those before operation. ConclusionPatientcontrolled epidural，intravenous and subcutaneous analgesia can be effective to relieve postoperative pain in total hysterectomy patients.Furthermore patientcontrolled epidural analgesia （PCEA）is superior to patientcontrolled intravenous analgesia（PCIA）and subcutaneous analgesia（PCSA）in inhibiting postoperative stress reaction.
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  麻醉科用药专栏

  Total Intravenous Anesthesia Induced by Sufentanil and Propofol in Galactophore Operations

  SHEN Yingyan;ZHOU Qinghe;XIAO Wangpin

  2008, 27(9): 1052-1054.

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  ABSTRACTObjectiveTo evaluate the efficiency and safety of total intravenous anesthesia induced by sufentanil and propofol in galactophore operations. MethodsSixty patients with ASA grade Ⅰ～Ⅱ who will be performed galactophore operations were randomly divided into two groups：sufentanil group（group SF,n=30）and fentanyl group（group F,n=30）. Total intravenous anesthesia was induced by midazolam 0.04 mg•kg1,propofol 3 μg•mL-1 and sufentanil 0.3 μg•kg-1 in group SF and midazolam 0.04 mg•kg1,propofol 3 μg•mL-1 and fentanyl 3 μg•kg-1 in group F. Propofol was used until operation completion . The variation of blood pressure, heart rate and respiration were assessed at pre and pro inducation and discussion, as well as the consciousness recovery was analyzed after operation. ResultsBoth choke and myotonia were observed in 2 groups. The decreasing of heart rates and blood pressure were significantly in group F than that in group SF (P＜0.05). Respiration depression of patients in group F was more markedly than that in group SF(P＜0.05). The quality of revival from anaesthesia was no difference in two groups. Conclusion It is a better method of using total intravenous anesthesia induced by sufentanil and propofol in galactophore operations with steady hemodynamics and slight respiratory depression.
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  麻醉科用药专栏

  A Study on Preemptive Analgesi of Lornoxicam Combined with Midazolam for the Patients Receiving Breast Tumor Resection

  FU Weidong;LEI Lipei

  2008, 27(9): 1054-1056.

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  ABSTRACTObjectiveTo observe the effect of preemptive analgesia and sedation of lornoxicam combined with midazolam for breast tumor resection . Methods60 patients (ASAⅠ～Ⅱ) undergoing breast tumor resection with local anesthesia, half an hour before the surgery,were randomly given lornoxicam 16 mg(group A) or lornoxicam 16 mg combined with midazolam 0.06 mg•kg1（group B）and normal saline 4 mL(group C) by intravenous injection. Visual analog score(VAS) was selected as the index of painintensity.Analgesic dosage, dosing frequency, index of respiratory and circulation system, Ramsay sedation score and the advese effect ware recorded. ResultsCompared with normal saline group,VAS of the other two group were lower, and had less volume and frequency(P＜0.05); The index of hemodynamics kept stable in the group B and its Ramsay sedation score was highest(P＜0.05). ConclusionUsing lornoxicam combined with midazolam for preemptive analgesia and sedation was satisfied.
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  麻醉科用药专栏

  Halfeffective Plasma Concentration of Sufentanil Resulted in Respiratory Depression during Brachial Plexus Block Anesthesia for Elderly Patients

  QIU Jianxin;DONG Yeping;XIAO Wangpin

  2008, 27(9): 1057-1058.

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  ABSTRACTObjectiveTo determine the halfeffective plasma concentration (EC50) of sufentanil to cause respiratory depression during brachial plexus block anesthesia for elderly patients. MethodsTwenty patients aged 6575 years，with gradeⅠorⅡASA, undergoing elective operations were enrolled. TCI sufentanil was given by “up and down” method for 30 minutes, started form target plasma concentration of 0.1 μg•L-1 .And the ratio of two close plasma concentrations was 1.2. RR, SpO2, PETCO2 and arterial gas analysis were monitored before and during operation. ResultsThe EC50 of sufentanil was 0.20 μg•L-1.The 95% confidence level was 0.18－0.22 μg•L-1. ConclusionThe halfeffective plasma concentration of suifentanil to cause respiratory depression was 0.20 μg•L-1.
药物与临床
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  药物与临床

  A Controlled Study of Olanzapine or Qeutipine Combined with Lithium in the Therapy of Bipolar Mania

  SHEN Zhongxia;CHEN Haizhi;YANG Shenguo;JIANG Yonghong

  2008, 27(9): 1072-1073.

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  ABSTRACTObjectiveTo study the efficacy and safety of olanzapine and qeutipine combined with lithium in the treatment of bipolar mania. Methods60 patients of bipolar mania according CCMD3 criteria were randomly assigned into olanzapine group (olanzapine 10－20 mg•d-1）and qeutipine group( qeutipine 200－750 mg•d-1), and both groups were combined with lithium 750－1 250 mg•d-1. The effectiveness and the side effects were evaluated by BRMS and TESS respevtively for 6 weeks. ResultsThe olanzapine group had a quicker effect than qeutipine group while the total effectiveness was equivalent. The side effects have no significant difference. ConclusionBoth olanzapine and qeutipine combined with lithium were effective and tolerable in the treatment of bipolar mania.
药物制剂
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  药物制剂

  The Preparation of Metformin Hydrochloride Pellets with Centrifugal Granulation Technique

  WANG Xiaoli;LI Xiaoming;LIU Jicheng

  2008, 27(9): 1092-1094.

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  ABSTRACTObjectiveTo prepare metformin hydrochloride pellets with centrifugal granulation and powder layering technique. MethodsMetformin hydrochloride and MCC(microcrystalline cellulose) pellets were prepared by powder layering technique with the centrifugal granulation equipment and the quality was evaluated by yield ratio, the size distribution, sphericity of pellets, bulk density ,friability,and release ratio. ResultsThe processing variables were optimized as followed: the ratio of metformin hydrochloride and Lactose and MCC were 100：5：3. The preparation of metformin hydrochlorid was satisfactory under the condition mentioned above . Conclusion The metformin hydrochloride pellets had a good appearance, uniform granulation diameter, standard rigidity, appropriate fluidity and high dissolubility , without skeletal structure.
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  药物制剂

  A Study on Preparation of Orally Disintegrating Tablets of Andrographolide

  WANG Jianhong;CHEN Zhende;YANG Hongying;JIA Mengliang;XIE Li;SUN Yehong

  2008, 27(9): 1095-1097.

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  (1. ABSTRACTObjectiveTo prepare orally disintegrating tablets of andrographolide and establish a quality control standard. MethodsThe tablet was prepared by direct powder compression technique. The testing method of dissolution was established, and the content of andrographolide was determined by HPLC.ResultsThe disintegrating time of the orally disintegrating tablets was eligible and the dissolution uniformity was excellent. ConclusionThe orally disintegrating tablets of andrographolide without irritate were more considerable in the absorption, effective time compared with the conventional tablets.
药品质量控制
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  药品质量控制

  Determination of Salvianolic Acid B in Xinli Capsule by HPLCELSD

  XIN Ying;SONG Xiaodong;LIU Zhe;ZHANG Channa;HUI Rutai

  2008, 27(9): 1102-1103.

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  ABSTRACTObjectiveTo establish an HPLCELSD method to determine the level of salvianolic acid B in xinli Capsule. Methods HPLCELSD system consisting of a Kromsil C18（4.6 mm× 250 mm，5 μm） and a mixture of methanolacetonitrileformic acidwater（30：10：1：59）as the mobile phase was adopted. The column temperature was set at 35 ℃．The UV detection wavelength was set at 286 nm．ResultsA closed linearity relation ( r = 0.999 9) was shown while salvianolic acid B was ranged from 0.145 5 to 0.727 5 μg. The average recovery rate was 99.04%. The content of salvianolic acid B per capsule varied from 0.51 to 0.52 mg in three batches of xinli capsule．ConclusionThe method of HPLCELSD with excellent accuracy and reproducibility was appropriate for the quality control of xinli capsule.
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  药品质量控制

  Determination of Organochlorine Pesticide Residues in Hirudo and Hippocampus

  ZHANG Linlin;XUE Jian;XU Duowu;PENG Fei

  2008, 27(9): 1104-1106.

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  ABSTRACTObjectiveTo establish an analytical method to detect 17 organochlorine pesticides in hirudo and hippocampus, and to investigate the degree of pesticide residues in hirudo and hippocampus. MethodsSamples were purchased from medicinal material market countrywide and sonicated with a mixture of acetone and petroleum ether (1：1,V/V). The extract was cleaned with sulphuric acid and detected by GC (DB1701fusedsilica capillary column) equipped with a 63Ni electroncapture detector (ECD). ResultsThe method was suitable for determination of 17 organochlorine pesticide residues in hirudo and hippocampus. The recovery ratio was 75.3% and 88.1% ,respectively. The content of DDT in hirudo was higher than that in hippocampus. ConclusionThe pesticide residues in animal medicinal materials should be taken into account.
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  药品质量控制

  Studies on the Related Substances of Ribostamycin Sulfate for Injection

  LI Xiaoxin;JIANG Hong;ZHAO Yaping;CHEN Ninglin;MA Changqing

  2008, 27(9): 1106-1108.

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  (1. ABSTRACTObjectiveTo establish a method to determine the related substances of ribostamycin for injection. MethodsThinlayer chromatography(TLC) was develop using the silica G as sorbent,a mixture of 2butanone, methanol,isopropanol,ammonia water and water(10：12：3：8：2)as developing solvent.The samples were colored by 0.2% triketohydrindene hydrate dissolved in the nbutanol and saturated by water. ResultsAn apparent separation between the principal spot of ribostamycin and the related spots was observed. The detectability was 0.08 μg. ConclusionThe method is simple for the quality control of the preparation with a high specificity.
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  药品质量控制

  Fingerprint Profile of Corydalis humosa Migo. by HPLC

  SHI Jing;WANG Wei;CHEN Liurong

  2008, 27(9): 1108-1110.

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  (1. ABSTRACTObjectiveTo establish an HPLC method to map fingerprint profile Corydalis humosa Migo. MethodsAn RPHPLC method was used with gradient elution and a Symmetry C18 column (4.6 mm×150 mm,5 μm).The mobile phase A was acetonitrile, and the mobile phase B was 0.1% phosphoric acid(adjusted to pH to 6.00 with triethylamine).The detective wavelength was at 220 nm.ResultsNine common peaks were confirmed in fingerprints.The methodological evaluation showed that the RPHPLC had a good repeatability. The fingerprints from different parts of Corydalis humosa Migo. were diverse.ConclusionHPLC was a simple and precise method to identify fingerprint of Corydalis humosa Migo.,moreover provided an experimental base for further quality control.