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    药物研究
  • 药物研究
    HPLC Fingerprint Study on Middle Polarity Part of Chaenomeles Fruit
    TAO Junyan;ZHANG Xiaoyu;HUANG Zhijun;XIONG Fuliang;ZHANG Qiongguang;XIAO Fei
    2008, 27(5): 494-496.
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    ObjectiveTo study the fingerprint of middle polarity part from Fruit of Chaenomeles at different time and in different process methods, which could provide GAP basis for planting and reasonable application. MethodsHPLC with UV detector was used to analyze the patterns of fruit of Chaenomeles. Coffeic acid and chlorogenic acid were used as standard substances, C18（Kromasil C18 10 nm－5 μm,4.6 mm×250 mm）column was used with a mobile phase of (A) acetonitrilemethonal (6：4)(B) 0. 05 %phosphoric acid gradient elution , 0－12 min (10 %－30%A, 90%－70 %B) ,－30 min (30%－50 %A ,70%－50%B) ,－35 min (50%－70 %A,50 %－30%B),－45 min (70%－90%A ,30%－10%B) ,－50 min (90%－60 %A ,10%－40%B) ,－60 min(60%－10%A , 40%－90%B) , ～70 min(10%A ,90%B);The flow rate was 0.8 mL•min-1 . The wavelength of detector was set at 283 nm. ResultsThe fingerprint of fruit of Chaenomeles was showed an excellent correlation. Samples of Chaenomeles fruit collected from different time were divided into 3 types, 1－4 shared 2 common peaks, 5－7 shared 3 common peaks,8 and 9 had 3 common peaks. Collecting time of the 5th bath is the best time for the 5th batch;the typical peak volume was the largest for the process of drying in the sun.ConclusionThe study is helpful for the quality control, the gathering and application of Chinese flowering quince.
  • 药物研究
    Distribution of Polysaccharide MDG-1 after Single Intravenous Dose in Rats
    LU Zhiling;FENG Yi;XU Desheng;LIN Xiao
    2008, 27(5): 497-499.
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    ObjectiveTo establish a method for measuring polysaccharide MDG1 from tissues by precolumn FITC labeledHPGPC and investigating the distribution of it in rat tissues. MethodsVarious tissues were obtained at 5,10,30 and 60 min, respectively, after a single intravenous administration of 50 mg•kg-1 polysaccharide MDG1. The concentration of MDG1 in samples were subsequently determined by HPGPC. ResultsMost of MDG-1 was readily accumulated in kidneys and excreted in urine. The distribution sequence of different tissues ranking from high to low was lung, stomach, heart, spleen, liver, brain. ConclusionMDG1 could exert antimyocardial ischemia effects directly on the target organ.
  • 药物研究
    Effects of Puerarin on the Activities of Cytochrome P450Enzyme in vitro
    WANG Qibin;DI Wei;CHENG Xiaoli;ZHANG Penghua;TU Ziliang
    2008, 27(5): 500-504.
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    ObjectiveTo investigate the effects of puerarin on the activities of cytochromes 1A2 (CYP1A2)、CYP2C9、CYP2C19、CYP2D6 and CYP3A4 in vitro.MethodsCaffeine, tolbutamide, mephenytoin, metoprolol and midazolam were incubated as probes with or without puerarin respectively to study the effect of puerarin on different cytochromes P450 activities. The concentrations of probe drugs and their metabolites were determined by HPLC, and the activities of cytochrome P450 were measured by the recombinant enzyme reaction system. ResultsPuerarin had no significant effects on the activities of CYP2C9, CYP2C19 and CYP3A4; however, the lower concentration (0.1 mmol•L1) decreased the activities of CYP1A2 by （48±9）%(P＜0.05), CYP2D6 by （61±8）%(P＜0.01); the higher concentration （0.4 mmol•L-1）decreased the activities of CYP1A2 by （82±8）%(P＜0.01),CYP2D6 by （88±6）%(P＜0.01). ConclusionPuerarin remarkably inhibits the activity of CYP1A2 and CYP2D6 in a dosedependent fashion in vitro. This finding might benifit for the study of drug interaction and the mechanism related to puerarin.
  • 药物研究
    Effect of Colquhoumia Root on TGF-β1and MCP- Expression in Experimental Mesangial Proliferative Glomerulonephritis
    CHANG Wei;SUN Hanying;ZENG Hongbing;LV Yongman
    2008, 27(5): 505-508.
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    ObjectiveTo investigate the renoprotective effects of colquhoumia root on experimental mesangial proliferative glomerulonephritis (MsPGN) in rats. MethodsMsPGN was induced in Wistar rats by i.v. the rabbit antirat thymocyte serum(ATS). Rats were randomly divided into the normal control, model, and treatment group. After14 days of therapy, 24 hours’ urinary protein was measured. Rats were sacrificed, histopathological changes were observed by PAS staining; RTPCR and Western blot techniques were used to evaluate the mRNA and protein expression of TGFβ1and MCP1 in the kidney tissues, respectively. ResultsColquhoumia root reduced 24 hours’ urinary protein, inhibited the expression of TGFβ1 and MCP1 (P＜0.01, respectively) compared with the model group, and accompanied with the significant improvement in renal pathological changes. Compared with normal group, no significant difference was shown (P＞0.05). ConclusionColquhoumia root improves pathological changes in MsPGN, remarkably suppresses mesangial cell proliferation and extracellular matrix accumulation, which may be associated with downregulation the expression of TGFβ1and MCP1.
  • 药物研究
    Effects of Radix Astragali on Pathological Change and Expression of TGFβ1 in Renal Tissues of Left Renal Vein Constriction Rats
    HE Xiaofeng;ZHAO Xiaoqin;LIU Xiaocheng;LIU Wei;SHAO Jufang
    2008, 27(5): 508-510.
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    ObjectiveTo investigate the effect of radix astragali on renal pathology and fibrosis factor expression on left renal vein constriction rats, and to provide the experimental basis on the drug therapy for the left renal vein entrapment syndrome.Methods SD rats were randomly devided into 3 groups,shamoperation group,model group,and radix astragali treated group.The rat model of left renal vein constriction was established by left renal vein partial deligation operation.Rats were treated with radix astragali. After 6 weeks,all animals were killed. Renal tissue was used to observe pathological change and to detect TGFβ1 expression. ResultsPathological change of left renal tissues was more severe, and TGFβ1 expression increased significantly in the model group compared to that in shamoperation group.Treated with radix astragali could lessen pathological damage and decrease TGFβ1 expression.ConclusionPathological change as mesangial cell proliferation,tubular cell atrophy,mesenchymal inflammatory cell infiltration and fibrosis could appear on left renal vein constriction rats.Radix astragali has protective effect against these pathological changes by reducing TGFβ1 expression.
  • 药物研究
    Empirical Study of Jingu Tablet on Promoting Blood Circulation and Removing Blood Stasis
    OU Li;GUO Jiansheng
    2008, 27(5): 513-514.
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    ObjectiveTo study the blood circulation promoting and blood stasis removing effects of jingu tablet. MethodsThe blood stasis model on rats was created by combining the adrenalin and icebath, and effects of Jingu tablet on hemorheology and changes of auricle microcirculation were investigated. ResultsJingu tablet decreased the whole blood viscosity,improved coagulating condition in the acute blood stasis model, and reduced hematocrit. It also enhanced auricle microcirculation obviously. Compared with the blank group, there was a significant difference among low, middle, and high dose of jingu tablet 20 min after treatment. ConclusionJingu tablet has effects on promoting blood circulation and removing blood stasis.
  • 药物研究
    Multidrug Resistance Reversion by Nimesulide on Gastric Cancer Cell Line SGC7901VCR
    CHEN Jinhui;CHEN Qingqing;CHEN Li
    2008, 27(5): 515-517.
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    ObjectiveTo evaluate the effect of nimesulide on gastric cancer cell line SGC7901VCR and SGC7901 and explore the mechanism of which reversing multidrug resistance to gastric cancer cell line SGC7901VCR. Methods Cell suppression rates were detected by MTT assay. Rh123 concentration in cells and expressions of P170, GSTπ were analyzed by flow cytometry. ResultsNimesulide inhibited the cell growth of SGC7901VCR and SGC7901 in a dose and time dependent manner, especially for SGC7901 cells. Rh123 fluorescence intensity in the SGC7901VCR cell was obviously increased and P170、GSTπ expressions were decreased by nimesulide(P＜0.05). ConclusionNimesulide can partly reverse multidrug resistance to gastric cancer cell line SGC7901VCR, the mechanism of which may be associated with decreasing expressions of P-170 and GST-π.
  • 药物研究
    Effect of PNS on Collagen Synthesis in HSCT6 Cells
    XUE Qing;XUE Shaoli;WANG Yasong;ZHANG Jing;WANG Yi;XIA Mizhen
    2008, 27(5): 517-519.
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    ObjectiveTo establish a model of collagen synthesis in vitro and to probe the effect of PNS on that.MethodsHSCT6 cells were incubated with the mixed sera containing different concentrations of NBS and rat sera, so as to establish a mixed seradriven collagen synthesis model. PNS was studied on the collagen synthesis model by 3HProline incorporation method. Results10% NBS with 3% rat serum was the best condition for stimulating the collagen synthesis in HSCT6 cells . After 48 and 72 hours’ incubation, 0.01,0.05,0.25,1.00 and 1.25 mg•mL1 PNS significantly suppressed the collagen synthesis in HSCT6 cells (P＜0.05) in a concentration and time dependent manner(F=4.517 and 5.832,P＜0.05）. ConclusionA mixed seradriven collagen synthesis model has been successfully established, and PNS markedly inhibits collagen synthesis in HSCT6 cells.
  • 药物研究
    Antimyocardial Hypoxic Effects of Extract from Sophora Flavescens Ait
    XU Xiaoyan;WANG Xiaodan;XIN Xiaoming;SUN Qinghai;GAO Yunsheng;ZHU Yuyun
    2008, 27(5): 520-521.
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    ObjectiveTo study the effect of extract from sophora flavescens ait. on hypoxia tolerance in hypoxic cardiomyocyte mice. MethodsThe model of myocardium anoxia increase were induced by i.p. injected isoprenaline. Recorded the accumulative oxygen consumption at different time and the survival time of mice. ResultsAfter intragastrically administered with aqueous extract of sophora flavescens ait.at the dose of 10 and 20 g•kg1 respectively, the survival time were prolonged obviously and the oxygen consumption were reduced. ConclusionAqueous extract of sophora flavescens ait. Remarkablly enhances the hypoxia tolerance in hypoxic cardiomyocyte mice in a dose related manner.
  • 药物研究
    Preparation and Release of Dexibuprofen Suppositories in vitro
    XIE Bin;DING Yufeng;HE Guofang
    2008, 27(5): 522-525.
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    ObjectiveTo prepare dexibuprofen sustained release suppositories and evaluate its drug release characteristics in vitro. MethodsThe optimal formulation and preparation technology of the suppositories were found with orthogonal design, which was used to prepare and detect the release of suppositories in vitro as well. ResultsThe released rate of the prepared suppositories was consistent with the Higuchi equation and drugs could be sustained released in vitro over 8 hours. ConclusionThe formulation is reasonablly designed, and the preparation technology is feasible to carry out and the method of quality control is reliable to use.
    • 儿科用药专栏
  • 儿科用药专栏
    1,25-Dihydroxyvitamin D3 is a Negative Regulator for the Activation of Angiotonin
    ZOU Minshu;NIE Guoming;YU Jian;HE Weixun;LUO Liman;XU Hongtao
    2008, 27(5): 530-533.
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    ObjectiveTo investigate the influence of 1,25dihydroxyvitamin D3［1,25(OH)2D3］ on the activation of the renin angiotensin aldosterone system(RAAS) in rats with diabetes mellitus(DM), and the protection on podocyt.MethodsSD rats were divided into four groups, normal control group(group A), DM group(group B), angiotensin Ⅱ(Ang Ⅱ)group (group C), and AngⅡcombined with 1,25(OH)2D3(group D) group. DM rats were induced by i.p. streptozotocin(STZ). Group C was embedded with the osmotic minipump for giving 400 ng•kg1•min1AngⅡ. Group D was given 400 ng•kg1•min1AngⅡand 450 ng•kg1•min11,25(OH)2D3 by two osmotic minipumps. Blood glucose(BG),contractive pressure(SBP),24 h total proteinuria (TP),the activation of renin, the level of AngⅡand aldosterone(Ald) were measured after 10 week’s treatment. The podocalyxin(PCX, podocytespecific marker) was detected to identity the urinary podocytes(UPC) and the distribution of glomerular epithelial cell membrane protein1(GLEPP1) was observed by immunofluorescene. ResultsBG,SBP,UPC,TP,PRA,AngII and Ald in group B were significantly higher than those in group A. SBP,UPC,TP,PRA,AngⅡand Ald in group C were significantly raised compared with those in group B （P＜0.05 or P＜0.01）. SBP,UPC,TP,AngⅡand Ald in group D were significantly lower than those in group C. AngⅡin group D was significantly lower than that in group B. Histologically, the distribution of GLEPP1 in glomeruli was normal in group A by immunofluorescene, obviously absence in group C, and absence in group B and D. The positive correlations were found between the levels of UPC with TP(rs=0.51,P＜0.01) and AngⅡ (rs=0.35,P＜0.05). Conclusion1,25(OH)2D3 may be a negative regulator for the reninangiotensin system by downregulating the activity of RAAS to prevent the defluvium and excretion of podocyte, which mediating its renal protective effects.
    • 药物与临床
  • 药物与临床
    Systematic Evaluation of the Therapeutic Efficacy of Rhubarb on Severe Acute Pancreatitis
    CHEN Xiangjian;ZENG Qiqiang;WANG Haibo;HUANG Yingpeng;JIN Rong;HAN Shaoliang
    2008, 27(5): 549-552.
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    ObjectiveThe aim of this study is to evaluate the effectiveness of rhubarb in the treatment of severe acute pancreatitis according to a systematic review and metaanalysis. MethodsThe articles published on May of 1966~2007 about the randomized controlled clinical trials of rhubarb on severe acute pancreatitis were searched for. According to the criteria of selection and exclusion ,twelve studies were enrolled into the analysis. The details about the trial designing, characters of the subjects, results were reviewed by two independent evaluators and analyzed by using Revman 4.2 software. ResultsCompared with the control group, patients treated with rhubarb were showed significantly shorter period of bowel sound recovery (WMD=－2.40,95%CI:－3.70～－1.09,P＜0.01) , lower time of the first defecation (WMD=－4.73,95%CI:－6.58～－2.89,P＜0.01) , quicker stomachache relief (WMD=－5.30,95%CI:－11.23～－0.63,P＜0.01), less hospital stay (days)（WMD=－13.03,95%CI:－19.73～－6.33,P＜0.01）and lower hospital death (O r=0.35,95%CI: 0.17～0.76,P＜0.01). ConclusionThe systematic review and metaanalysis shows that the adjunctive therapy with rhubarb can decrease the bowel sound recovery time, the first defecation time, the stomachache relief period, hospital stay (days) and hospital death.
    • 药学进展
  • 药学进展
    胡斌1,李延2,肖轶雯3
    2008, 27(5): 573-575.
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    • 药物制剂
  • 药物制剂
    Preparing Microencapsulation of Zedoary Turmeric Oil by Spray Drying Method
    ZHAO Yanhua;FU Hongxing;LIN Shengzhao;HU Shuping
    2008, 27(5): 581-584.
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    ObjectiveTo investigate the best formulation and technics for preparing zedoary turmeric oil microcapsule. MethodsAcacia gum (AG) and maltodextrin (MD) as capsule wall material was used to entrap the oil by spray drying method. Influence of the weight ratios of AG/MD、the amount of assistant emulsifier、dry matter concentrations (DM)、the ratios of core and capsule wall material on microcapsulation was studied by using embedding ratio(ER) as a standard and orthogonal designing was applied to investigate the technique. ResultsThe best weight ratio of AG /MD was 4：1,with 1 mL of span80,40% DM,at the ratio of core and capsule wall material as 4 mL：10 g. The best condition for spraydry technique : inlet temperature at 160 ℃; feeding rate as 2.5 mL•min-1; aspiration as 100%. By which, the average size could be 5.2 μm and the ratio of embed(ER) could reach 94.2% with a stable quality. ConclusionThe zedoary turmeric oil microcapsules prepared by the established methods are round and full,with a tight surface ,the satisfying ER and stability.
    • 药品质量控制
  • 药品质量控制
    Determination of Oleanolic Acid and Ursolic Acid in Oldenlandia Diffusa (Willd.) Roxb. by LC/MS
    YANG Yihua;CHEN Yuxiang
    2008, 27(5): 589-591.
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    ObjectiveTo establish a method of determining the contents of Oleanolic Acid and Ursolic Acid in Oldenlandia Diffusa (Willd.) Roxb simultaneously. MethodsLCMS was applied, with a C18 column, Diode array detector and methanol plus glacial acetic acid at the ratio of 75 to 25 as a mobile phase. The flow rate was 0.2 mL•min-1 and column temperature was set at 45 ℃. The mass spectrometry detector was equipped with an APCI source and a selective cation flow monitoring mode. ResultsAverage recoveries of two acids were 98.87 %( RSD＝3.09 %) and 100.78 %（RSD＝1.68%）,respectively. ConclusionThe method is rapid,simple,accurate and reproducible, which is suitable for the qulity control of Oldenlandia Diffusa (Willd.) Roxb.
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