Archive

• 01 April 2008 Volume 27 Issue 4

    

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  药物研究
  感染性疾病用药专栏
  药物与临床
  药物制剂
  药品质量控制
  用药指南
  

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药物研究
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  药物研究

  Inhibition of Sophora Alopecuoride Alkaloid Derivative SPRIDA on H22 Liver Cancer of Mice

  HONG Ge;LIU Peixun;SHEN Xiu

  2008, 27(4): 369-371.

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  Objective To investigate the inhibition and doserelated effect of sophora alopecuoride alkaloid derivative on H22 liver cancer of mice. Methods Mice were transplanted with H22 liver cancer, and divided into 5 groups, 15 in each. The control group was treated with pure water 10 mL•kg^-1•d^-1, the low, middle and high group was given SPRIDA 150,300,600 mg•kg^-1•d^-1, respectively. The positive group was administered with cisplatin 4 mg•kg^-1. The mice were all treated for consecutive 10 d. Results The inhibition rate of three groups was 33.44%, 41.90%, 48.25%, respectively. No significantly sideeffects on the index of the liver,spleen and thymus were found in each group;Nevertheless,the obvious decrease in the index of the liver,spleen and thymus was shown in the positive group. Conclusion SPRIDA has strong therapeutic effect on H22 liver cancer without affecting the immunological function of mice bearing tumor, which suggests to be a low toxicity antitumor new drug.
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  药物研究

  Effect of Ruhuang Pill on the Activity and GFAP Expression of Rat CRL-2541 Cells under High Ammonia

  YAN Xue-sheng;PENG Ya-qin;ZHANG Chi-zhi

  2008, 27(4): 371-373.

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  Objective To explore effects of ruhuang pill on the activity and GFAP expression of rat astrogia CRL-2541 cells under high ammonia. Methods The cultured CRL-2541 cells were divided into control group, amonia injured group, normal CSF group, CSF group and Ruhuang pill group. Different solutions were used in each group. The activity of CRL-2541 was detected by MTT test and the expression of GFAP was analyzed by immunologic cell staining method. Results The CSF of ruhuang pill increased the absorbance of CRL-2541 cells injured under the high ammonia and upregulated the GFAP expression. Conclusion Ruhuang pill could enter in BBB and protect the injured astroglia in high ammonia, which suggests that the target of antiHE would lie in astroglia.
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  药物研究

  Analysis of the Volatile Oil from Taohong Siwu Decoction by GC/MS

  JU Yi-chun;WU Lu-ling;LI Xiang;CHEN Jian-wei;LI min

  2008, 27(4): 374-376.

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  Objective To analyze the main constituents of volatile oil from taohong siwu decoction. Methods Volatile oil was obtained by steam distillation technique. The chemical constituents were separated and identified by GC/MS. Results 134 peaks were shown from the volatile oil, in which 65 chemical constituents were identified, which accounting for 83.469% of the volatile oil. Conclusion The main constituents in the volatile oil are tetradecanoic acid, 1(3H)-isobenzofuranone, 3-butylidenebenzaldehyde, nhexadecanoic acid, dodecanoic acid and 9-Oc-tadecenoic acid, which provides the basis for the further study of active components from taohong siwu decoction.
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  药物研究

  Fingerprint of Eucommia ulmoides Oliv by HPLC

  HUANG Ping;XI Xian-rong;ZHANG En-jing;ZHANG Qiong;HAN Shuang

  2008, 27(4): 376-378.

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  Objective To study a fingerprint of raw material of Eucommia ulmoides Oliv from various habitats by HPLC. Methods XDB-C₁₈ column (150 mm×4.6 mm,5 μm)was used,with mixtures of acetonitrile and 1% acetic acid as mobile phase in a gradient mode. The flow rate was 1 mL•min^-1 and wavelength was 277 nm. Results According to the optimum chromatographic conditions,a good fingerprint of Eucommia ulmoides Oliv were described out. Conclusion The method is simple and accurate with good reproducibility, which provides a scientific basis for controlling the quality of Eucommia ulmoides Oliv.
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  药物研究

  Influence of Ubidecarenone on the Immune Function of Mice

  XIA Yong;ZHANG Shuang-feng;HUANG Bai-fen;ZHAO Rao-xian;YAN Jun;HUANG Liang

  2008, 27(4): 379-382.

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  Objective To investigate effects of ubidecarenone on the murine immune system. Methods Mice were i.g. given with 4.2－25 mg•kg^-1 for 30 days. The splenocyte proliferation, delayed type hypersensitivity, serum antihemolysin, antibodyforming cells, chicken red cells phagocytosis by macrophages, carbon clearance and natural killer(NK) cell function were investigated after administration. Results Compared with the control group, both spleen lymphocytes proliferation and delayed type hypersensitivity reaction were improved significantly; the number of hemocytolysis plaque was increased, the macrophage’s activity and NK cell function were enhanced as well（P＜0.05）．No apparent differences of carbon clearance and antihemolysin were observed among groups（P＞0.05）. Conclusion Ubidecarenone posses the strengthening effects on immune system.
  
  
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  药物研究

  Influence of Ubidecarenone on the Immune Function of Mice

  XIA Yong;ZHANG Shuang-feng;HUANG Bai-fen;ZHAO Rao-xian;YAN Jun;HUANG Liang

  2008, 27(4): 379-382.

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  Objective To investigate effects of ubidecarenone on the murine immune system. Methods Mice were i.g. given with 4.2－25 mg•kg^-1 for 30 days. The splenocyte proliferation, delayed type hypersensitivity, serum antihemolysin, antibodyforming cells, chicken red cells phagocytosis by macrophages, carbon clearance and natural killer(NK) cell function were investigated after administration. Results Compared with the control group, both spleen lymphocytes proliferation and delayed type hypersensitivity reaction were improved significantly; the number of hemocytolysis plaque was increased, the macrophage’s activity and NK cell function were enhanced as well（P＜0.05）．No apparent differences of carbon clearance and antihemolysin were observed among groups（P＞0.05）. Conclusion Ubidecarenone posses the strengthening effects on immune system.
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  药物研究

  Quantification of Plasma Valsartan and Hydrochlorothiazide in Healthy Volunteers after Administration of the Valsartan Complex

  SI Lu-qin;FAN Zhao-ze;HUANG Jian-geng;LI Gao

  2008, 27(4): 383-385.

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  Objective To establish a method for determining the plasma level of hydrochlorothiazide and valsartan by HPLC in healthy volunteers after administration of compound valsartan dispersible tablets. Methods Plasma samples of hydrochlorothiazide and valsartan were determined by using RPHPLC Methods with ornidazole as the internal standard for hydrochlorothiazide and candesartan as the internal standard for valsartan. The HPLC condition for hydrochlorothiazide was that Eurospher-100 C₁₈ (250 mm×4.6 mm, 5 μm); mobile phase：methyl cyanidewater(30：70,V/V);flow rate：1 mL•min^-1;column temperature：RT;detection wavelenth: 272 nm;loading volume：50 μL. The HPLC condition for valsartan was that Eurospher-100 C₁₈ (250 mm×4.6 mm, 5 μm);mobile phase：methanol0.02 mol•L^-1  potassium dihydrogen phosphate (70：30,V/V),pH 2.40;flow rate：1 mL•min^-1;column temperature：RT;wave length：excitation wave 265 nm,emission wave 395 nm;loading volume：20 μL. Results Standard curve of hydrochlorothiazide was linear in the concentration range of 5 to 1 000 ng•mL^-1 (r＝0.999 5), and valsartan of 0.025 to 10.000 μg•mL^-1 (r＝0.999 5). The detection limit of hydrochlorothiazide and valsartan was 2.5, 10 ng•mL-1, respectively,and the intraday RSD and interday RSD of hydrochlorothiazide vs. valsartan were 1.42%－5.22% vs. 0.52%－3.30% and 2.62%－9.04% vs. 2.00%－9.01% (n=6) , respectively. Conclusion The method was found to be convenient, sensitive, accurate and fine reproducible for determination the concentration of hydrochlorothiazide and valsartan in plasma.
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  药物研究

  Concentrations Determination of Clozapine in Human Plasma by HPLC

  WU Lan;ZHU Jun;LIU Wen-xian;LIU Wei-zhong;WANG Hua-cheng

  2008, 27(4): 386-388.

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  Objective To establish a method for determining the concentration of clozapine in human plasma by HPLC. Methods The drug from plasma was analyzed in a reverse phase HPLC system C18 column ( 150 mm×4.6 mm, 5 μm) ; mobile phase consisted of 0.03 mol•L^-1ammonioummethanol (25：75); the flow rate was 0.8 mL•min^-1;the detection wavelength was at 254 nm. Ethyl acetate and dichloromethane(80：20) was used as extracting solvent. Results The average recoveries of clozapine in high, middle and low concentrations(1000.0, 400.0, 10.0 ng•mL^-1) were 98.28%, 97.63% and 101.41%, respectively. The intraday and interday variation (RSD) was less than 7%（n=5）. The calibration curve of clozapine showed good linearity, r=0.999 7(n=10), over the range of 10.0－1 000.0 ng•mL^-1.The regression equation was C=25.69F+3.47,r=0.999 7(n=10). The minimum detectable concentration of clozapine was 5.0 ng•mL-1. Conclusion The method is sensitive, accurate, easy and quick, which can be used for clinical drug monitoring and pharmacokinetics studies.
感染性疾病用药专栏
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  感染性疾病用药专栏

  Anti-HBV Effects of TTF Contained Sera in Vitro

  YANG Ke;ZHENG Zuo-wen;OU Xian-hong

  2008, 27(4): 390-392.

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  Objective To investigate the antiHBV effects of TTF extracted from Guangxi Teng Tea. Methods The inhibition of TTF contained sera on 2.2.15 cells expressing HBsAg and HBeAg were studied by MTT assay. Results TTF contained sera significantly inhibited HBsAg and HBeAg secreted from 2.2.15 cells. Conclusion TTF contained sera inhibit the HBsAg and HBeAg secretion from 2.2.15 cells in a doserelated manner, which indicates that TTF posses the antiHBV effect.
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  感染性疾病用药专栏

  Toxibility and Effect of the Antiviral Herb Compound on Influenza Virus and Respiratory Syncytial Virus in Vitro

  YANG Dao-feng;XU Li;SHAO Jing-fang;ZHANG Yue;GONG Fei-li

  2008, 27(4): 392-394.

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  Objective To investigate the antivirus activity of the antiviral herb compound on influenza virus and respiratory syncytial virus(RSV). Methods The suppression on influenza virus by different doses of antiviral herb compound in MDCK passage cells was evaluated by hemaglutinin titration test, while the effect on RSV was studied by the neutralization test in Hep-2 cells.ResultsNo obvious toxic effects on MDCK cells and Hep-2 cells were observed by the compound with the concentration lower than 1.5 mg•mL^-1. However, it suppressed the hemagglutination of influenza virus and the aggression of RSV on target cells at the concentration over 0.15 mg•mL^-1. Conclusion The antiviral herb compound shows potent inhibition on the influenza virus and RSV in vitro with a high safety.
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  感染性疾病用药专栏

  Anti-Herpes Effects of the Extract from Indigowoad Leaf in Vitro

  YU Shu-qing;CHEN Xiang-yi;YU Ling

  2008, 27(4): 394-396.

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  Objective To study effects of the extract from indigowoad leaf on herpes simplex virus (HSV)-Ⅱin vitro. Methods Using Vero cell as host cells and acyclovir as positive control, the cytotoxicities on cells by the herbs, the direct virus destroying and inhibition on virus proliferation were investigated by adopting MTT assay and observing cytopathic effect (CPE). Results It was showed that the herbs had no direct inactivation on HSV-Ⅱin vitro, no inhibition on virus absorption either. However, the herbs could inhibit HSV-Ⅱ biosynthesis and multiplication. The treatment index (TI) was 3.56. Conclusion The extracts from indigowoad leaf show some anti HSV-Ⅱ effects in vitro by suppressing virus replication in cells.

药物与临床
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  药物与临床

  Comparing Study of Mirtazapine and Amitriptyline on Treating Depression with Chronic Pain

  ZHU Yi-ping

  2008, 27(4): 408-411.

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  Objective To compare the efficiency and side effects of mirtazapine and amitriptyline on treating depression accompanied with chronic pain. Methods 68 out-patients consistent with the diagnostic criteria of depression and chronic pain simultaneously were divided into the treatment group with 36 and control group with 32. Patients were treated with amitriptyline at the range of 0.2－0.8 mg•d^-1. The treatment group was added with mirtazapine 15 mg, 30 mg,or to 45 mg if necessary, respectively once in the supper. The control group was added with amitriptyline 50,100 and 150 mg at the first, fourth and seventh day, respectively, during lunch and supper. HAMD,HAMA,TESS,VAS were assessed before treating and 7, 14, 28 days after treating. Results No significant difference of HAMD,HAMA,VAS existed between two groups before treatment. After 7 days’ treatment, the score of HAMD,HAMA,VAS in the treating group decreased rapidly, the significant difference was found（P＜0.01）compared with those before treating（P＜0.01）. And the score was obviously lower than the control group(P＜0.05,P＜0.01）. After treating for 28 days, scores of HAMD,HAMA,VAS of both groups were significantly different from those before treatment（P＜0.01）. But scores of HAMD,HAMA,VAS between two groups showed no difference（P＞0.05）. The difference of efficiency between two groups was not obvious（P＞0.05）. The total score of TESS in the treatment group was different or significantly different from that in the control group（P＜0.05,P＜0.01）. Conclusion Mirtazapine could treat depression accompanied with chronic pain. It not only relieves the symptoms of depression and anxiety, but also improves or eliminates the symptoms of pain with relatively mild side effects.
药物制剂
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  药物制剂

  Studies on the Technique of the Alcoholic Extraction of Ganzhixiao Capsule

  SONG Zhen－guo;WEI Shi－chao;FENG Yao;LU Fu－er

  2008, 27(4): 436-438.

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  Objective To optimize the alcoholic extraction technique of ganzhixiao capsule. Methods The total contents of anthraquinone from ganzhixiao capsules were regarded as the indicator, and L9(34) orthogonal study was applied to observe the concentration and amount of alcohol, extracting time and times of extraction in order to determine the best extracting technique. Results The times of extraction and the concentration of alcohol were the most significant factors, and the optimal extraction process required 70% alcohol, 7 times amount of solvent, 3 times extraction, 2 h for each. Conclusion The Results show that the extraction technique was stable, reliable and eligible for manufacturing instruments.
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  药物制剂

  Preparation of and Solubility Determination of Azithromycin Dispersible Tablet

  ZHANG Li－e;ZHUGE Zhong－xi

  2008, 27(4): 438-440.

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  Objective To develop a preparation process for dispersible tablets of azithromyin. Methods Dispersible tablets of azithromycin were prepared by using azithromycin as principal agents with hyprolose and croscamellose sodium as supplementary materials, and the solubitiy of which was determined by UV spectrophotomery. Results The quality controllable solubility determination was developed through the research on the content, linearity, stability, recovery rate, reproducibility, limitation of solubility and homogeneity for dispersible tablets of azithromycin . Conclusion The technology of preparation for dispersible tablets of azithromycin is simple, stable, well dispersed and quick.
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  药物制剂

  Optimization of Extraction Process for Baibixiao Qintment

  ZHANG Hong;ZHANG Geng;CHEN Jing－ping

  2008, 27(4): 441-443.

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  Objective To optimize the extraction process of baibixiao qintment. Methods The optimum extraction process including water volume, decocting time and times and capacity of alcohol was determined by using the L₉(3⁴) orthogonal test and regarding contents of emodin,chrysophanol and matrine as parameters. Results The optimum process was that decocting for 3 times, 1.5 h by each with water 8 times volume of herbal weight, and precipitating with the equivalent alcohol. Conclusion The optimized extracting conditions is reasonable and practicable.
药品质量控制
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  药品质量控制

  Determination of Astragaloside IV in the Gel Compound of Astragaloside by HPLC－ELSD

  LIN Zhi－cheng;LIU Miao－na;ZHANG Li－dong;YAN Shu－chao;YIN Chun－ping

  2008, 27(4): 455-457.

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  Objective To establish a method for determining the concentration of astragaloside IV in the gel compound of astragaloside. Methods The HPLC－ELSD was used, with the analytical column: a WelchromC₁₈(4.6 mm×250 mm,5 μm); the mobile phase : acetonitrilewater(46：54); the flow rate: 0.5 mL•min^-1; the column temperature: 30 ℃. The detecting parameters were that the temperature of drift tube for ELSD was 105 ℃ and the flow rate of N2 was 2.7 L•min^-1. Results The calibration curve was linear over the range of 4.24－8.48 μg(r=0.999 2). The average recovery was 101.57%,and RSD was 2.17% (n=6). Conclusion The proposed method could be used in the quantitative analysis of astragaloside IV in the gel compound of astragaloside and the method is convenient,economical and accurate with a good reproducibility.
用药指南
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  用药指南

  The Dosage Regimen Design of Bolus Intravenous Administration Based on Microsoft Excel

  SU Yin－fa;DU Le－yan

  2008, 27(4): 471-473.

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  Objective To establish a method of dosage regimen design of multiple dosing intravenous administration. Methods Microsoft Excel was used to make a program on the pharmacokinetic parameters to show the dosage regimen. Results After inputting the pharmacokinetic parameters,dosing interval (τ) ,preliminary or maintenance dose (d),and time after drug administration, the Excel spreadsheet displayed plasma concentration at any time,area under curve （AUC）, and loading dose after the administration. The time of the effective plasma concentration （tec）,the minimum dose （dmin）and maximum dose （d_max） were obtained by program solution on Excel. Conclusion This design method can provide a safe and effective dosage for clinical administration and evaluate a dosage regimen.
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  用药指南

  Compatible Stability of Dexamethasone Sodium Phosphate and Penicillin G Injection

  XU Xian－e;XIN Xue－jun;CHEN Fang－liang;LIANG Zhen－ye

  2008, 27(4): 473-476.

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  Objective To study the compatible stability of penicillin G sodium with dexamethasone sodium phosphate injection. Methods 3.2×106 unit of penicillin G was combined with serial doses of dexamethasone in 5% glucose injection or 0.9% sodium chloride injection. Within 6 hours after mixing, the peak height was determined by HPLC and the absorption was determined by ultraviolet spectrophotometry under room temperature; the outward appearance was observed and the pH value of the mixture was determined. Results The outward appearance of the penicillin G combined with dexamethasone was clear and the pH value of the mixture was ascended with the dose of dexamethasone increased within 6 hours, and approached neutral. Both the absorption curve and the absorption under the maximum wave length were not changed significantly within 6 hours by ultraviolet spectrophotometry. No significant influence on the peak of penicillin G was found by dexamethasone, while the mild stabilization effect was observed 3 hours later by HPLC, especially for 40 μg•mL^-1 of dexamethasone［about 10 mg•(250 mL)^-1］. Conclusion There is not incompatibility between penicillin G and dexamethasone in 5% glucose or 0.9% sodium chloride injection, and dexamethasone has the mild effect on the stability of penicillin G.