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    药物研究
  • 药物研究
    SHENG Yanmei;XIE Xingliang;YANG Ming;XU Runchun
    2008, 27(3): 258-261.
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    ABSTRACTObjectiveTo filtrate a new herbal medicine recipe of yuchangning ,which composed by the effective ingredients for treating UC,and explore a new research idea to develop herbal medicines. MethodsUC model rats led by TNBS were treated with variant proportions contained radix astragali and raix sophorae flavescentis and the most prefered recipe was filtrated . And then to evalulate the effect difference between the common recipe composed of medicinal marerials and the most prefered recipe. ResultsBased on preliminary research results, the most preferred prescription was radix astragali 15 g, raix sophorae flavescentis 15 g. Moreover, we found that the curative effect of two herbs combination was better than that of anyone and especially, 1:1 rate of radix astragali and raix sophorae flavescentis showed a most strong curative effect. The curative effect between the prescription composed of common medicinal marerials and the the most prefered prescription were similar, suggesting that astragalus saponin, astragalus polysaccharide,matrine and oxymatrine possiblely were effective components in this prescription.The prescription composed of astragalus saponin 25 mg, astragalus polysaccharide 239 mg, matrine 50 mg and oxymatrine 150 mg each day was reasonable. ConclusionsA novel herbal medicine recipe——yuchangning ,which composed of effective components, was invented and this provided a logical strategy for developing new herbal medicine recipes.
  • 药物研究
    WU Yiyan;LI Li;YANG Zhi;CAI Yuting;LIANG Yihong;FENG Qinxi;YU Chunyan;YANG Fenhua
    2008, 27(3): 262-264.
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    ABSTRACTObjectiveTo study the effect of tongjingshu oral liquid on the smooth muscle constriction and the level of prostaglandins F2α from the uterus of rats under dysmenorrhea situation. MethodsDysmenorrhea was induced by diaethy lstilbestrol subcutaneous injection, or cloprostenol or oxytocin celiac injection. After tongjingshu oral liquid gastric perfusion, the frequency and range of the smooth muscle constriction were determined, and the level of the prostaglandins F2α was detected. ResultsThe smooth muscle constriction resulted from such as prostaglandins F2α or oxytocin was able to be strongly relaxed by tongjingshu oral liquid,which also might decline the level of the prostaglandins F2α in the smooth muscle.ConclusionTongjingshu oral liquid is a effective agent to relieve the dysmenorrhea and protect from the primary dysmenorrheal in the rats.
  • 药物研究
    LIU Ying; LU Fuer;HUANG Guangying
    2008, 27(3): 265-267.
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    (1. ABSTRACTObjectiveTo establish insulin resistant model of HepG2 cell in vitro induced by high insulin level and then to investigate the effects of radix salviae miltiorrhilza on insulin sensitivity in the insulin resistant cells. MethodsHepG2 cells were devided into 4 groups and incubated. GroupⅠtreated with 5×107 mol•L-1 insulin for 16 hours is to establish insulin resistant model, and GroupⅡ without insulin is a control. Insulin resistant cells were treated in group Ⅲ with radix salviae miltiorrhilza and groupⅣ with rosiglitazone . The insulinstimulated glucose incorporation rate was determined with 3HDglucose uptake test. ResultsThe incorporation rate of 3HDglucose in insulinresistant HepG2 cells was significantly lower than that in control cells(P<0.01). The glucose uptake of the insulin resistance cells was able to be outstandingly increased by rasix salviae miltiorrhiza treatment, compared with that of control cells(P<0.05) .ConclusionHepG2 cells exposed to 5×107 mol•L-1 insulin for 16 hours were able to obtain a state of insulin resistance. Radix salviae miltiorrhiza could improve the insulin sensitivity in the cell model and attenuate the aggression of insulin resistance.
  • 药物研究
    WU Zecheng;LIU Shenwei
    2008, 27(3): 268-271.
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    ABSTRACTObjectiveTo explore the effect of valsartan on recruitment of monocyte/macrophage cells in renal tissue of type 2 diabetic rats. MethodsStreptozotocin induced diabetic rats were randomly assigned to three groups: control group, diabetic model group and diabetic model with valsartan treatment group (10 mg•kg1•d1). After 8 weeks, the expression of MCP1 and CD68 in the renal tissue of type 2 diabetic rats was detected by immunohistochemistry. In addition,BG, Scr, BUN, Ccr and UAER were measured respectively. ResultsCompared to diabetic model group, the level of UAER, Scr,BUN,kidney/weigh in valsartan treated group were significantly decreased(P< 0.05) . The expression of MCP1 and CD68 in the renal tissue of type 2 diabetic rats were increased in diabetic model group than that in control group (P< 0.01). Valsartan could significantly downregulate the expression of MCP1 and CD68 in the renal tissue of diabetic rats (P<0.05). ConclusionValsartan was able to ameliorate the renal lesion in type 2 diabetic rats,through a potential mechanism partly underlain the inhibition of recruitment of monocyte/macrophage cells in renal tissue of type 2 diabetic rats.
  • 药物研究
    CAI Jun;XU Wenwei;ZHANG Hong;HU Xiao;CHENG Xiaohua;WEN Jinhua;XIONG Yuqin
    2008, 27(3): 271-274.
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    ABSTRACTObjectiveTo investigate pharmacokinetic and bioequivalance of bisoprolol hemifumarate dispersed tablet in healthy volunteers. MethodsTwenty volunteers were randomly divided into 2 groups. A single oral dose of 10 mg of test or reference tablets was delivered to each volunteer in an open crossover test. The plasma concentration of bisoprolol hemifumarate was determined by HPLC fluorescence detection. Based on the parameters of pharmacokintic and relative bioavailability, the bioequivalance of bisoprolol hemifumarate was evaluated. ResultsAUC0~48 h in 2 groups was (628.04 ±136.61 ) and (641.24 ±161.20) ng•h•mL1 respectivly; Cmax was 46.78±12.03) and (47.91±12.74) ng•mL1 in 2 groups; tmax was (2.4±0.8 h) and (2.1± 0.8) h ; t1/2 was (9.97±1.70) and (9.71±1.23) h in 2 groups. The relative bioavailability of bisoprolol hemifumarate dispersed tablet was (103.1±30.8) %. ConclusionThe two preparations of bisoprolol hemifumarate dispersed tablet and reference tablets are bioequivalent .
  • 药物研究
    WANG Xinhui;YANG Zhihong;ZHAO Dexue;HAN Yuen;YUE Wang
    2008, 27(3): 274-277.
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    ABSTRACTObjectiveTo study the cooperative antiemetic effects of extracts of capsicum and gingers in rats. MethodsThe rats were randomly divided into 4 groups and respectively administrated with cisplatin, apomorphine, rotation,or distilled water as the control.The consumption of nonnutritive kaolin was recorded in rats. The antiemetic effect of combined capsicum with gingers was observed in the emetic animal model. ResultsThe emetic behaviors of pica could be successfully induced by cisplatin(3 mg•kg1, ip), apomorphine(3.2 mg•kg1 ,sc) and rotation(100 r•min1,1 h). Cooperatively given extracts of capsicum and gingers in two groups could markedly inhibit kaolin intakes induced by cisplatin, apomorphine and rotation in a dosedependent manner (P<0.05). ConclusionsThe combination of extracts of capsicum and gingers showed an antiemetic effect in model rats,and gingers might decrease the gastrointestinal stimulus resulted from capsicum treatment alone. The mechanism may be the inhibitions of substance P 、5HT3 acceptors and the vomiting center in central nervous system.
  • 药物研究
    GENG Wuhong
    2008, 27(3): 278-279.
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    ABSTRACTObjectiveTo evaluate the safety of the cefpirome sulfate for injection. MethodsThe safety was assassed in animals by pharmacological assays such as allergy reaction test, venous and muscular stimulation test,and hemolysis test.ResultsNo allergy reactions, the hemolysis and significant stimulation on vessels and muscles were observed after using cefpirome sulfate. ConclusionCefpirome sulfate for injection is safe under the experimental condition.
  • 药物研究
    YU Daojun;DONG Xiaoqin;JIN Keqin;ZHOU Tianmei;CHEN Yueming;WU Shenghai
    2008, 27(3): 280-284.
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    (1. ABSTRACTObjectiveTo analyze the antibiosis effect of antibiotics combination on multidrug resistant Acinetobacter Baumarnii in vivo and in vitro. MethodsDrug resistant genetypes of 36 strains multidrug resistant acinetobacter Baumarnii were identified and the minimum bactericidal concentration (MBC) of 8 antibiotics to multidrug resistant acinetobacter Baumarnii were determined. The mice infected by multidrug resistant acinetobacter Baumarnii were treated by 8 antibiotics combination under the minimum inhibition concentration. ResultsDrug resistant genetypes of 36 strains multidrug resistant acinetobacter Baumarnii were OXA23.The MBC(μg•mL1) of tienem,tazocillin,suloerazon,rifampicin,ceftazidime,amikacin,ciprofloxacin , polymyxin E was 64,512,64,250,2 048,2 048,150,2,respectively. The antibiotics combination of tienem and amikacin ,tazocillin and rifampicin ,tienem and Rifampicin, could not inhabit multidrug resistant acinetobacter Baumarnii, but suloerazon combined with amikacin showed a sterilization . The protection rate of antibiotics combination of polymyxin E plus tazocillin,tazocillin plus amikacin and suloerazon plus amikacin was 100.0%,30.0%,90.0% and 90.0%,respectively. Mortality of the infectious mice treated with tienem and suloerazon was 100%. ConclusionsThere was obviously increased in MBC of common antibiotics for multidrug resistant acinetobacter Baumarnii. Reasonable combination antibiotics was available to therapy multidrug resistant acinetobacter Baumarnii, and was also able to reduce the dosage of agents.
  • 抗结核病药物专栏
  • 抗结核病药物专栏
    WANG Xinjing;WANG Wei;LI Li;ZHAO Yulan;YE Yixiu
    2008, 27(3): 285-287.
    (1. ABSTRACTObjectiveTo determine the concentration of rifampicin in lymph nodes of rats in order to provide an experiment foundation to improve regimens of chemothetapy for lymphoid tuberculosis. MethodsFifteen Wistar rats were averagely divided into 3 groups at random, and received rifampicin after 12 hours abrosia.Rats were killed at points of 2 hours for one group,8 hours and 24 hours for the other two groups respectively. The concentration of rifampicin in celio-lymphnode and serum was determined by a HPLC method. ResultsA significant linear relation of the rifampicin concentration between celiolymphnode and serum was dicovered(r=0.999 6). RSD of both intraday and inerday was less than 7%. The rifampicin concentration in lymphnode at each time point was higher than its MIC in vitro, but obviously lower than that in serum(P<0.01). ConclusionsThe HPLC is suitable method to detect rifampicin level in lymphnode with high veracity. It is essential to elevate the concentration of drug in lymph nodes to treat lymphoid tuberculosis.
  • 药物与临床
  • 药物与临床
    ZHANG Hanwang;CHEN Liping;YUE Jing
    2008, 27(3): 294-295.
    ABSTRACTObjectiveTo investigate the effect of spironolactone and cyproterone(diane35) in female patients with polycystic ovary syndrome (PCOS). Methods130 PCOS patients were randomly divided into two groups: treat group with pironolaetone combined with cyproterone and control group with placebo twice per day for 3 months. The levels of sexual hormones during pre or pro treatment, including FSH、LH、E2、PRL、T were analyzed in two groups.ResultsAfter three months of the treatment of spironolactone plus cyproterone , the ratio of luteinizing hormone (LH) and follicle stimulating hormone(FSH), The levels of LH were significantly decreased (P<0.01).Both ovulation rate and pregnancy rate increased significantly in treatment group. ConclusionThe combined cyproterone and spironolactone could strongly promote the ovulation and increase the pregnancy frequence in PCOS patients.
  • 药物与临床
    LUO Gaoping;REN Libing;LI Qifei;XIA Rui
    2008, 27(3): 296-298.
    ABSTRACTObjectiveTo evaluate the safety and efficacy of propofol patientcontrolled sedation(PCS) as a local anethesia in thyroidectomy .MethodsSixty patients(ASAⅠ~Ⅱ) undergoing thyroidectomy were randomly divided into group P(pcs,n=30) and group C(control, n=30). In group P, after 50 mg of propofol as a loading dose, the patientcontrolled sedation was induced by propofol using the Graseby9300PCA pump.Therapy procedurse were consisted of a lockout interval of one minute and a dose of propofol 10 mg, and 30 mg is the maximum dose in 5 minutes. Group C was only given pethidine 1 mg•kg1 and promethazine 0.5 mg•kg1. Systolic blood pressure(SBP),heart rate(HR), serum oxygen saturation(SPO2) were recorded before infusion,30 minutes after infusion and 5 minutes after operation. Sedation scores, cooperation degrees and satisfaction were also measured. ResultsThe SBP,HR and SpO2 in group P were significiantly different from group C at the 30 minutes after infusion. Sedation scores were lower in group P (P<0.01),while cooperation and satisfaction scores were higher than that in group C(P<0.05). ConclusionsPropofol patientcontrolled sedation is a safe and effective method with high satisfaction for thyroidectomy.
  • 药物与临床
    YAN Haifan;CHEN Lingling;ZHONG Yufen;MAO Junliang
    2008, 27(3): 298-300.
    ABSTRACTObjectiveTo investigate whether double dose of somatostatin may increase efficacy in controlling acute variceal bleeding. MethodsAfter receiving a bolus of 250 μg per hour somatostatin, a total of 79 liver cirrhosis patients with acute variceal bleeding were randomized into 2 groups and receive somatostatin infusion at 500 μg•h-1 (double dose group, n=39) or 250 μg•h-1 (standard dose group, n=40). ResultsThe control rate of bleeding in the double dose group was similar to that in the standard dose group (87.1% vs. 70.0%, P>0.05).The double dose group achieved a higher control rate of bleeding in the ChildPugh B+C grade patients (83.9% vs. 60.0%, P<0.05), less blood transfusion (2.4±2. 3) L vs. (3.9±3.2)L( P<0.05) than the standard dose group. ConclusionThe double dose somatostatin increased the control rate of acute variceal bleeding in the ChildPugh B and C grade patients.
  • 药物制剂
  • 药物制剂
    BAI Jie;HE Ying
    2008, 27(3): 315-318.
    ABSTRACTObjectiveTo prepare angelicae sinensis dispersible tablet with characteristics of conveniently application and quickly action. MethodsBoth diluents and disintegrants were screened. Direct powder compression technique was used to prepare angelicae sinensis dispersible tablet. The prescription was assessed by particularities of disintegration time and suspension stability. ResultsAn optimal prescription assembly was 50% of angelicae sinensis extract,34% of prosolv, 10% of cCMCNa, 5% of amylum pregelatinisatum,1% of colloidal silicon dioxide. The test showed that disintegration time was less than three minutes, both dispersible homogeneity and suspension stability were good, and dissolution rate at 15 minutes was more than 80%. ConclusionsThe technique was simple and reliable. The angelicae sinensis dispersible tablets with optimal prescription could meet the dispersible tablets requirements of Chinese Pharmacopoeia published in 2005.
  • 药品质量控制
  • 药品质量控制
    CAO Lin;ZHOU Zheng;MAO Yazhu
    2008, 27(3): 321-323.
    ABSTRACTObjectiveTo establish a method for determining the residues of nine kinds of organic solvents in imiquimod by headspace gas chromatography.MethodsChromatography analysis was carried out in a HPINNOWAX capillary column (30 m×0.32 mm,0.25 μm) with sequential increase of temperature programming. The injector temperature was 250 ℃, and the FID detector temperature was 270 ℃.ResultsNine kinds of solvents were separated completely. It appears a good linearity in the experimental concentration.The minimal detective levels of nhexane, acetone, THF, ethyl acetate, methanol, dichloromethane,ethanol, methylbenzene and DMF separately were 0.007 7, 0.051 0, 0.037 3, 0.263 3, 0.146 5, 0.314 5, 0.259 1, 0.046 3 and 0.452 1 μg•mL1. The RSD of precision were all less than 3.0%.The average recovery rates were 90% ~115%. ConclusionThe method is simple with high sensitivity and accurateness. Gas chromatography could be an appropriate technique to detect residual solvents in imiquimod.
  • 药品质量控制
    ZHAO Li;ZHONG Qiaoni;LEI Yuxia;CHENG Ming;ZHANG Changgong
    2008, 27(3): 323-325.
    ABSTRACTObjectiveTo map HPLC chromatography fingerprinting and measure the level of sarponin a & d of total sarponin from bupleurum Chinense DC. MethodsUsing a Hypersil C18 column(4.6 mm×250 mm,5 μm),a high performance liquid chromatography assay with gradient elution was developed to identify 10 samples at wavelength 210 nm. The chromatography conditions follow as :the column temperature was set up at 25 ℃. the samplesize was 10 μL at 0.7 mL•min-1 flow rate and a gradient solution of acetonitrile and water was consisted of 30%40% acetonitrile(020 min),40%50% acetonitrile(2035 min) and 50%60% acetonitrile(3545 min). ResultsThe chromatography fingerprinting from all of the 10 samples share a high similarity. With respect of the preparing process of the total saponin from bupleurum chinense DC, the validity and stability were significantly excellent. ConclusionThe method established in the present study is convenient, reliable, and could be used for the quality control of the extract of the total saponin from bupleurum chinense DC and for the monitor and control of the preparation process. Based on this method, a higher quality of total saponin would be assure with strongly consistency in each group productions.
  • 用药指南
  • 用药指南
    LI Kaijun;CHEN Fuchao;ZHU Jun;LI Yifan
    2008, 27(3): 332-333.
    ABSTRACTObjectiveTo study the stability of a mixture of levofloxacin hydrochloride injection and cemitidine injection in 5% glucose injection. MethodsThe contents of levofloxacin hydrochloride injection mixed with cemitidine injection were determined by UV spectrophotometry under the room temperature within 8 hours, and the appearance characteristics, pH and UV spectrum of the solution were analyzed. ResultsNo significant changes were found in terms of the appearance, pH, UV spectrum and the contents of the mixed solution. ConclusionThe mixture of levofloxacin hydrochloride with cemitidine in 5% glucose injection can be safely used under room temperature within 8 hours.
  • 用药指南
    SU Yinfa
    2008, 27(3): 333-336.
    ABSTRACTObjectiveTo estimate the pharmacokinetic parameters for drug obeying one order adsorption and MichaelisMenten elimination by SPSS(statistics package for social science, 12.0) software. MethodsConcentrationtime data obtained from four order RungeKutta algorism with excel forms were converted into △C/△t at a sere of sampling time. The dC/dt was approximately replaced by △C/△t,and concentration c was accounted by an arithmetic mean of(Ci+Ci+1). Data of △C/△tc were analogized by nonlinear regression of SPSS. ResultsThe pharmacokinetic parameters estimated by SPSS were accurate and reliable. ConclusionSPSS is suitable for estimating the pharmacokinetic parameters of drugs obeying one order adsorption and MichaelisMenten elimination.