中国科技论文统计源期刊　中文核心期刊  
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊  
日本科学技术振兴机构数据库（JST）
第七届湖北十大名刊提名奖  

Archive

  • Select all
    |
    药物研究
  • 药物研究
    Effects of Midazolam, Penehyclidine Hydrochloride on Memory Acquisition, Consolidation and Retrieval Processes in Mice
    YUAN Li-yong;DAI Ti-jun;WANG Xia-min;CHENG Fang
    2008, 27(1): 7-10.
    Download PDF ( )   Knowledge map   Save
    Objective To investigate the effects of midazolam and penehyclidine hydrochloride on memory acquisition, consolidation and retrieval processes of mice in stepthrough test. Methods According to stratified randomized block design,150 Kunming mice were divided into 7 groups: midazolam 1 mg•kg-1(group M, n=30), penehyclidine hydrochloride 0.2 mg•kg-1 (group P, n=30),midazolam 1 mg•kg-1+ penehyclidine hydrochloride 0.2 mg•kg-1 (group M+P, n=30) and control group(group NS,n=30); scopolamine 2 mg•kg-1 (group S, n=10) Sodium nitrite 125 mg•kg-1 (group SN, n=10) and 30%alcohol 0.1 mL•(10 g）-1(group A, n=10) as positive control groups. Stepthrough test was used to evaluate the effects of midazolam and penehyclidine hydrochloride on memory acquisition, consolidation and retrieval processes of mice. The latencies and the numbers of errors during testing section were recorded as the marks of learning and memory. The effects of midazolan and penehyclidine hydrochloride on memory acquisition, drugs administrated before training section to test memory acquisition. Midazolam, penehyclidine hydrochloride or normal saline was injected intraperitoneally 30 min before training section respectively. Positive drug scopolamine was injected intraperitoneally 15 min before training section. The effects of midazolan and penehyclidine hydrochloride on memory consolidation，drugs administrated immediately after training section to test memory consolidation.Midazolam, penehyclidine hydrochloride or normal saline was injected intraperitoneally immediately after training section respectively. Positive drug sodium nitrite was injected subcutaneously. The effects of midazolan and penehyclidine hydrochloride on memory retrieval, drugs administrated before testing section to test memory retrieval. Thirty min before testing section, midazolam was injected intraperitoneally, or positive drug ethanol was gavaged. Fifteen min before testing section, penehyclidine hydrochloride, or normal saline was injected intraperitoneally Results Midazolam impaired memory acquisition and consolidation when single administrated or associated with penehyclidine hydrochloride. Midazolam associated with penehyclidine hydrochloride did not worsen the effect on memory acquisition, but inhibited the effect on memory consolidation significantly. Midazolam alone or associated with penehyclidine did not impair memory retrieval processes. Penehyclidine hydrochloride alone did not impair memory acquisition,consolidation and retrieval processes. Conclusion The effects of midazolam and penehyclidine hydrochloride on learning and memory are advantageous for preventing awareness during surgical procedures.
  • 药物研究
    Effects of Allitridin on TGF-β1 in Murine Cytomegalovirus Infection
    YANG Zhu-feng;FANG Feng;SHU Sai-nan;ZHU Dan-dan;LI Ya-nan;LI Ge;DONG Yong-sui
    2008, 27(1): 11-14.
    Download PDF ( )   Knowledge map   Save
    Objective To study the effects of allitridin on the transcription and expression of TGF-β1 gene in the mice model with disseminative infection of murine cytomegalovirus (MCMV). Methods Sixty BALB/c mice were inoculated with MCMV Smith strains for establishing the model of disseminative infection, and randomly allocated into two groups: placebotreated group and allitridintreated group with a dose of 25 mg•kg-1•d-1. Another 60 mockinfected mice served as controls and were randomly allocated into allitridintreated controls and mockinfected controls. The mice were sacrificed for extraction of serum and splenocytes at 1 d, 3 d, 7 d, 14 d and 28 d after treatment, respectively. Inactive MCMV antigens were used as the stimulants for spleen cell culture in vitro. TGF-β1 levels in the serum and culture supernatant of splenocytes were measured by doubleantibody sandwich ELISA and TGF-β1 mRNA levels in the splenocytes were detected by RT-PCR assay. Results In placebotreated group, the TGF-β1 level in serum reached the peak at the 3 d, then went down gradually and was significantly higher than that of mockinfected controls at 28 d. The changes in TGF-β1 mRNA and protein expression in the splenocytes showed similar change, with significant increase at 3 d, going to peak at 7 d, returning to the level a bit higher than that of mockinfected group at 28 d. Comparing with placebotreated group, TGF-β1 levels both in serum and splenocytes’ supernatant were reduced after 3 days of treatment in allitridintreated group, showing most significant difference from 3 d to 7 d, meanwhile, TGF-β1 mRNA levels were nearly similar to the those of placebotreated group. Conclusion MCMV induce a higher transcription and expression of TGF-β1 gene in the acute phase of infection. However, allitridin can completely inhibit the transcription of TGF-β1 gene and significantly reduce TGF-β1 expressing levels in the serum and splenocytes’ supernatant induced by MCMV.
  • 药物研究
    Therapeutic Effects of Dauricine on Early Afterdepolariztions Induced by Dofetilide
    LIU Qiang-ni;YANG Xiao-yan;ZHANG Li;GONG Pei-li;ZENG Fan-dian
    2008, 27(1): 15-18.
    Download PDF ( )   Knowledge map   Save
    Objective To investigate the effects and possible mechanisms of dauricine (Dau) on early afterdepolarizations induced by dofetilide. Methods Cardiac hypertrophy was induced in rabbits by aortic banding; using conventional microelectrode techniques, the effects of dofetilide at the concentration of 1 μmol•L-1 on action potential duration(APD) and incidence of early afterdepolarizations (EADs) were studied and compared in rabbit papillary muscles between control and different pathological conditions, including hypokalemia and hypertrophy; the effects of Dau in concentrations of 30 μmol•L-1on APD and EADs were observed after EADs were induced by the pathologic conditions mentioned above. Results Compared with control, dofetilide significantly prolonged the APD of rabbit papillary muscle; the same phenomena were also found in hypokalemia and hypertrophy; dofetilide significantly advanced the incidence of EADs in hypokalemia concomitant with hypertrophy; Dau inhibited the prolongation of APD and significantly suppressed the incidence of EADs induced by dofetilide. Conclusion Dau exerts good therapeutic effects on early afterdepolarizations induced by dofetilide and these effects may be associated with inhibition of Dau on several ion channels.
  • 药物研究
    Effects of Ligustrazine on Bax Expression in Neurons of Rats with Epilepsy Induced by Penicillin
    YU Xiao-hong;ZHANG Duan-lian
    2008, 27(1): 18-20.
    Download PDF ( )   Knowledge map   Save
    Objective To study the effects of ligustrazine on Bax expression in cerebal neurons of rats with epilepsy induced by penicillin. Methods Through animal model of epileptic rat induced by penicillin employing BL-410 bio-functional experimental system to record the epileptic discharge of bilateral cerebral cortex, the effects of TMP (ip) on Bax expression in neurons of cerebral cortex of rats were ovserved . Results TMP groups(40 mg•kg-1,ip),compared with saline groups, had significant reducing effects on Bax expression in neurons of cerebral cortex. Conclusion Ligustrazine could significantly inhibit Bax expression in neurons of cerebral cortex in rats with epilepsies induce by penicillin.
  • 药物研究
    The Prevent Effects of Ulinastatin after Hepatic Ischemia and Reperfusion in Rats
    LIN Xue-zheng;PENG Cong-bin
    2008, 27(1): 21-23.
    Download PDF ( )   Knowledge map   Save
    Objective To investigate the effects of ulinastatin on hepatocyte mitochondrion after hepatic ischemia and reperfusion in rats. Methods Thirty SD rats were randomly divided into three groups: sham operation group (group C), hepatic ischemia and reperfusion group (group I) and ulinastatin intravenous injection group (group U). Total hepatic ischemia was induced for 30 min by clamping the hepatic artery and the portal vein using a microvascular clamp, then liver was reperfused for 120 min. After reperfusion, the levels of cytochrome C, ATP, superoxide dismutase (SOD) and malondialdehyde (MDA) levels in mitochondria of hepatic cell were determined. ResultsAfter reperfusion, the levels of cytochrome C, ATP and SOD in group I were lower than those in group C（P<0.01）, and these levels in group U were higher than those in group U（P<0.05 or P<0.01）. At the same time, the level of MDA in group I were higher than that in group C（P<0.01）, and this level in group U were lower than in group I（P<0.01）. Conclusion Ulinastatin has beneficial effects on hepatocyte mitochondrion after hepatic ischemia and reperfusion.
  • 药物研究
    Computer Virtual Screening Technology Applying to the Study of Functional Classification in Pharmacodynamic Molecules of TCM Prescription HuanglianDetoxicting Decoction
    LONG Wei;LIU Pei-xun;GAO Jing
    2008, 27(1): 23-25.
      Knowledge map   Save
    Objective To discuss the molecular microcosmic meaning of principle in traditional Chinese Medicine(TCM). Methods To classify the pharmacodynamic molecules in huanglian detoxiciting decoction by function, deploying the technology of computer virtual screening. Results According to the standard build in this experiment, 12 molecules of juncomponent, 152 molecules of chencomponent, 48 molecules of zuocomponent, and 53 molecules of shicomponent was found in 265 ingredient molecules in huanglian detoxiciting decoction. Conclusion The functional classification principle of junchenzuoshi rules the pharmacodynamic molecules in TCM, and the traditional principle of Chinese medicine, junchenzuoshi, do have the molecular microcosmic meaning.
  • 药物研究
    The Effects of Peristrophe Baphica on Immunological Function of Mice
    YANG Yi;WANG Zhen;LI Xu-mei
    2008, 27(1): 26-27.
    Download PDF ( )   Knowledge map   Save
    Objective To study the effects of Peristrophe baphica on immunological function of mice. Methods The indexes of the clearance rate of carbon particles and the serum hemolysin against SRBC were used to test the function of phagocyte and HC50 of mice. Results Compared with norma1 group, phagocytic index and HC50 content of Peristrophe baphica treated mice increased significantly. Conclusion Peristrophe baphica could enhance the immunological function of mice．
  • 药物研究
    In Vitro Experimental Study of Tumor Treated with Valeriana Iridoids
    ZHANG Shu-qin;XUE Cun-kuan;HE Xue-bin;LIU Zhi-gao
    2008, 27(1): 27-29.
    Download PDF ( )   Knowledge map   Save
    Objective To observe the antitumor effects of Valeriana Iridoids. Methods MTT method was adopted. The tumor cell lines were HL-60and K562.The growth condition, density and apperaence of tumor cell were observed under microscope. The A titres were detected and the rates of tumor inhibition were calculated. Results Experiment in vitro demonstrated that numbers of tumor cells in Valeriana Iridoids groups were significantly lower than those in 0.9%NaCl group, especially prominent in high dose group. The border of cells had crystalline body and a number of residual patch of cells. As compared with solvent group, there was significant or very significant difference(P<0.05 or P<0.01) between them. There was a positive correlation between the rate of tumor inhibition and the drug dose. Conclusion Valerinana Iridiods was shown to have a prominent antineoplastic effect in vitro.
    • 心血管系统用药专栏
  • 心血管系统用药专栏
    Effects of Tetrandrine on Ang Ⅱ-Induced Cardiomyocyte Hypertrophy and p-ERK1/2 Expression
    LIU Qiang;CHEN Hao
    2008, 27(1): 34-37.
    Download PDF ( )   Knowledge map   Save
    Objective To observe the effects of tetrandrine (Tet) on angiotensin Ⅱ (Ang Ⅱ)induced cardiomyocyte hypertrophy and the expressions of phosphorylated ERK1/2 (p-ERK1/2). Methods In the primary culture of neonatal rat cardiomyocytes, as indexes of cardiomyocyte hypertrophy, cell size was assessed by Texas Red-X phalloidin immunocytochemistry and protein synthesis rate was measured by ［3H］-Leucine incorporation. ERK activity was measured by ERK immunoprecipitation reagent box. The expressions of p-ERK1/2 was assessed using Western blot. Results Tet significantly inhibited Ang Ⅱinduced elevations of the cell size and protein synthesis rate. The expression of p-ERK1/2 and its activity showed a dosedependent inhibitory effect. Conclusion The anti-hypertrophic effect of Tet on Ang Ⅱinduced cardiomyocyte hypertrophy was associated with inhibition of ERK1/2 signaling pathway.-regulated kinase
  • 心血管系统用药专栏
    Effect of Cilnidipine on Blood Pressure in Patients with Hypertension Assessed by Ambulatory Blood Pressure Monitoring
    QI Hong;YANG Tian-lun;WU Sheng-bin;JING Shi-zhan
    2008, 27(1): 37-39.
    Download PDF ( )   Knowledge map   Save
    Objective To assess the antihypertensive effect of cilnidipine in patients with essential hypertension. MethodsAfter 2 weeks washout , 22 patients with mild to moderate essential hypertension were treated by openlabel method with cilnidipine capsule 5 mg qd for 2 weeks. Dosage was titrated to 10 mg qd for another 6 weeks in patients when DBP ≥90 mmHg. Both ambulatory blood pressure monitoring (ABPM) and laboratory test were performed after washout time and at the end of 8 weeks treatment. Results There were significant differences(P<0.05 or P<0.01) in the reduction of 24 h , daytime (6：00－22：00) , nighttime (22：00－6：00) average SBP or DBP, daytime systolic blood pressure load and nighttime diastolic blood pressure load. The T/P ratio in SBP and DBP is 0.61 and 0.50, respectively. The incidences of adverse effects were very low. ConclusionCilnidipine capsule 5 mg or 10 mg once daily is effective and safe in patients with essential hypertension.
  • 心血管系统用药专栏
    Therapeutic Efficacy of Carvedilol on Silent Myocardial Ischemia and Heart Rate Variability in Coronary Heart Disease
    XIA Jian-yuan;JIA Lian-wang
    2008, 27(1): 39-42.
    Download PDF ( )   Knowledge map   Save
    Objective To explore the therapeutic efficacy of carvedilol on silent myocardial ischemic(SMI) and heart rate variability (HRV) in coronary heart disease. Methods Seventyeight cases of coronary heart disease were randomly divided into treatment group (40 cases) and control group (38 cases). Control group was treated with aspirin, pravastatin and sorbitrate, and treatment group was treated with carvedilol based on aspirin and pravastatin. The changes in SMI and HRV were observed by Dynamic electrocardiolgram before and 12 weeks after treatment. Results Frequency and sustained time of symptomatic and asymtomatic myocardral ischemia were decreased significantly in two groups (P<0.01 and P<0.05, respectively) after treatment, but more significantly (P<0.05) in treatment group than in control group. HRV parameters (SDNN,SDANN, SDNNindex) were significantly improved (P<0.01) in the treatment group , but there were not different (P>0.05) in control group before and after treatment. No side effects were found in treatment with carvedilol. Conclusion There is significant therapeutic efficacy of carvedilol on SMI and HRV in coronary heart disease.
  • 心血管系统用药专栏
    Nicardipine Combined with Carvedilol in the Treatment of 21 Cases of Renal Hypertension
    HE Xiao-feng;ZHANG Hu;LIU Xiao-cheng
    2008, 27(1): 43-45.
    Download PDF ( )   Knowledge map   Save
    Objective To observe the therapeutic effect and safety of nicardipine combined with carvedilol on renal hypertension． Methods Fortynine renal hypertension patients were randomly divided into two groups. Patients in the control group were treated with nicardipine 80 mg q 8 h, while patients in treatment group nicardipine 40 mg q 8 h and carvedilol 25－50 mg qd for 8 weeks. Blood pressure, heart rate, renal function were detected before and after treatment． Results Total efficiency of lowering blood pressure in the control group was 67.86%, and that in the treatment group was 80.95%. There were no significant changes in heart rate, and renal function before and after treatment in two groups with no obvious adverse events． Conclusion Nicardipine combined with carvedilol was efficient and safe for the treatment of renal hypertension, which was more efficient than nicardipine alone.
    • 药物与临床
  • 药物与临床
    The Clinical Observation of Diabetes Mellitus Following Cisplatin-based Chemotherapy on Head and Neck Cancer
    FENG Jue-ping;FANG Jing;LIN Mei;WANG Ya-ping;LI Min;YU Xue-feng;YU Shi-ying
    2008, 27(1): 51-54.
    Download PDF ( )   Knowledge map   Save
    Objective To explore the association between cisplatinbased chemotherapy and diabetes mellitus in 144 patients with locally advanced head and neck cancer. Methods A retrospective study was carried out through analysis of fasting glucose levels in 144 patients with head and neck cancer. Results Six patients with diabetes mellitus prior to chemotherapy were excluded. A total of 138 patients were analyzed. Nine patients with DM (6.5%)were discovered during the chemotherapy, among whom 7 patients had normal blood glucose and 2 had IFG before chemotherapy. In 7 patients (5.1%)with impaired fasting blood glucose（IFG） before the initiation of chemotherapy, two were exacerbated into diabetes mellitus (DM), but remains still keep on IFG. The fasting glucose levels of 9 DM patients before the initiation chemotherapy was markedly lower, compared with that during the chemotherapy period (5.45±0.59 vs 11.44±4.52,P<0.01). Median cisplatin cumulative dose up until the diabetes occurred was 320 mg&#8226;(m2)-1,and median cycles until the appearance of hyperglycemia were 4 cycle. Conclusion Roution doses of CDDP used against head and neck cancers might be a significant risk factor involving in MD development. It was important that blood sugar levels in patients receiving chemotherapy with a cisplatinbased regimen should be assessed regularly.
    • 药物制剂
  • 药物制剂
    Optimization of Extracting Conditions of Zhijuzi by Alcohol
    WEN Wei;ZHANG Hong;ZENG Rong
    2008, 27(1): 77-79.
    Download PDF ( )   Knowledge map   Save
    Objective To select the optimizal extracting conditions of zhijuzi by alcohol. Methods To screen the extracting dissolvent on the basis of extractive yield and total flavonoids. To ascertain the extracting conditions by investigating the SOD activity and the concertration of MDA in rats liver homogenate. The extracting process was determined by orthogonal design. Results The optimal extracting condition was heat reflux for 2 hours using 8- fold 75% alcohol. Conclusion The extracting technology selected in this experiment is reasonable, pactical and conrtollable.
RSS