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    药物研究
  • 药物研究
    WU Shen-bao;ZHOU Guo-xiong;HUANG Jie-fei;ZHANG Hong;XIAO Ming-bing;JIANG Feng;LI Feng
    2007, 26(2): 121-124.
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    ABSTRACT Objective To probe into the effect of zileuton, a special inhibitor of 5-lipoxygenase (5-LOX), on the proliferation and apoptosis of pancreatic cancer cells in vitro. Methods The pancreatic cancer cells SW1990 were incubated in 12-well plates with zileuton in concentrations of 40,30,20,10,5,3 and 1 μg·mL-1 for 72 h, respectively. The cancer cells cultured under the same condition but without the addition of zileution served as the control. Cells harvested at different time points after the beginning of the culture were subjected to assay of cell proliferation with the MTT test, apoptosis with the AnnexinV/PI staining followed by flow cytometry(FCM) and cell cycle with FCM as well. Results After a 48 h incubation with zileuton at concentrations of 40 and 30
    μg·mL-, the proliferation of the pancreatic cancer cells were strongly inhibited as compared with that of cells of the control group (P<0.01). 72 h after the beginning of incubation with zileuton at concentration of 40,30 and 20 μg·mL-1, the cancer cells were shown to undergo a striking inhibition of proliferation as compared with those of the control group(P<0.01). Other results also showed that zileuton could inhibit the proliferation and promote apoptosis of the SW1990 cells in a concentration-time-dependent manner. After 18 h of treatment of the cells with zileuton, the ratio of the cancer cells blocked in the G0/G1 phase was increased (P<0.01) while that of the cells in the G2/M phase and S phase was decreased, as compared with the cells in the control group. Conclusion Zileuton was shown to inhibit proliferation and induce apoptosis of pancreatic cancer cells and induce blocade of the cells in the G1 phase seemingly by blocking the 5-lipoxygenase pathway of the cells.
  • 药物研究
    HAN Zihua;WENG Zhiliang;YU Zhixian;WU Xiuling;WANG Tianji;LU Sibao;LI Chengdi
    2007, 26(2): 124-126.
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    ABSTRACT Objective To probe into the inhibitory effect of polyvinylpyrrolidone(PVP) on human urinary bladder carcinoma cells T24 in vitro. MethodsHuman urinary bladder carcinoma cells T24 were incubated in medium containing PVP in final concentrations of 2.5%, 5.0% and 7.5%, respectively, for 72 h. The MTT test was used to assay the inhibitory effect of PVP on the T24cells while flow cytometry was adopted to examine the influence of PVP on the cell cycle and adhesive property of the carcinoma cells. ResultsThe inhibition rates of the cell growth were(15.11±2.36)% and(49.57±7.07)%, when the cells had been incubated with 2.5% PVP for 24 and 72 h, respectively. The rates increased to(35.42±5.55)% and(79.66±19.92)% when the cells had been incubated with 7.5% PVP for 24 and 72 h, respectively. After the T24 cells had been incubated with 5% PVP for 24 and 72 h, the ratios of cells is G0/G1 phase were(74.17±0.91)% and (46.69±3.76)% and those in G2/M phase cells were (14.63±0.47)% and (41.88±1.50)%, respectively. 5% PVP could also enhance the effect of murine IgG1 to adhere to the T24 cells. ConclusionPVP was shown to inhibit the growth and proliferation of human urinary bladder carcinoma cells T24 in vitro probably by blocking the cell cycle at the G2/M phase and serving as adhesive carrier of chemotherapeutic agents.
  • 药物研究
    ZHANG Youting;GE Niyun;WU Chengyun
    2007, 26(2): 127-130.
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    ABSTRACTObjectiveTo probe into the effect of Larginine on the rabbit lung injured by ischemia/reperfusion. MethodsThirty male rabbits were randomly divided into three equal groups: the sham operation group (group S), ischemiareperfusion group (group IR) and Larginine + ischemiareperfusion group (group LArg+IR). Rabbits of the LArg+IR group were given each an IV injection of 100 mg·kg1 of Larginine. A model of ischemiareperfusion injury of the left lung was then set up in each of the rabbits of group IR and group LArg+IR group by clamping(60 min) and declamping(60 min) of the hilus of the left lung under IV urethan anesthesia and mechanical ventilation. The same operation was performed on each of the animals of group S except for that the hilus of the left lung was not subjected to clamping/declaming. Venous blood samples from each of the rabbits of the 3 groups were taken before the operation, at the termination of ischemia (group IR and group LArg+IR) or 60 min after the operation (group S) and at the termination of reperfusion (group IR and group LArg+IR) or 120 min after the operation (group S), respectively, for the determination of the activity of plasma superoxide dismutase (SOD), content of plasma malonyldialdehyde(MDA), nitric oxide(NO)and calcitonin generelated peptide(CGRP). ResultsAt the termination of ischemia and reperfusion, the activity of SOD and content of NO in rabbits of the LArg+IR group were significantly higher than those in animals of group S or group IR(P<0. 01,P<0.01), while the activity of SOD and content of NO were significantly lower in rabbits of group IR than those in animals of group S( P<0. 05,P<0.05). At the termination of reperfusion, the content of MDA in rabbits of group IR was obviously higher than that in those of group S and group Larg+IR while the content of CGRP was significantly lower than that in rabbits of group S and group LArg+IR(P<0.01,P<0.01). ConclusionLArginine was shown to exert a protective effect on the lung submitted to an ischemiareperfusion injury in rabbits probably by inducing the activity of SOD and increased production of CGRP.
  • 药物研究
    LIU Lu;DU Guang;FANG Jianguo
    2007, 26(2): 130-132.
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    ABSTRACTObjectiveTo compare the effects of 2 preparations of Lycium Barbarum glycopeptide(LBG) on the proliferation of murine splenic lymphocytes. MethodsSuspensions of splenic lymphocytes harvested from BALB/C mice were prepared with the conventional methods. The suspensions were then separately added to the 2 samples of LBG in concentrations of 500,100,10 and 1 μg·mL1, respectively. (One of the LBG was prepared in the laboratory, the other was an industrial product). The blank controls were added to mediums without reagents. Each of all the cell suspensions was equally divided into 2 groups, to one of which was added 10 μg·mL1 of ConA, serving as positive control. The cells were then cultured for 72 h at 37 ℃ in an incubator containing 5%CO2. Flow cytometry and MTT colorimetry were separately used to assay the proliferation of the cells. ResultsThe results of flow cytometry revealed that both of the 2 preparation of LBG could enhance the proliferation of the murine splemic lymphocyles and both of them showed synergetic effects with ConA. MTT colorimetry demonstrated that LBG prepared in the laboratory could significantly promote the proliferation of murine splenic lymphocytes as compared with the controls (P<0.01). The LBG as an industrial product was shown to enhance the lymphocyte proliferation only in relatively higher concentrations. Only in relatively high concentrations did the LBG prepared in the laboratory show synergic effects with ConA while the industrially produced LBG manifested definite synergic effects with ConA(P<0.05). ConclusionBoth of the LBG preparation were shown to be provided with definite immunocompetence.
  • 药物研究
    WANG Yangtian;WANG Jian;ZHAO Ming;DI Hongjie
    2007, 26(2): 133-135.
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    ABSTRACT Objective To probe into the relationship between the activation of the transcription factor nuclear factorkappa B (NF-κB) and expression of inducible nitric oxide synthase (iNOS) and the treatment of diabetic nephropathy with glurenorm in rats. MethodsForty SpragueDawley rats were randomly divided into 4 equal groups: the control group(group A) ,diabetes model group(group B),glibenclamide treatment group(group C) and glurenorm treatment group(group D). A model of diabetes was set up in each of the rats of groups B,C and D by a single intraperitoneal injection of 60 mg·kg-1 of streptozotocin(STZ). Rats of group C and group D were then given each 1 mg·kg-1 of glibenclamide and 10 mg·kg-1 of glurenorm, respectively, administered by gastrogavage b.i.d., for 12 consecutive weeks. Rats of group A were given each an equivalent volume of 0.1 mmol·L1 of citric acid buffer solution, administered by intra peritoneal injection, b.i.d., for 12 consecutive weeks. The animals were sacrificed at the end of the 12th week and the kidneys were taken for histopathological examination and assay of the activity of NFκB and expression of iNOS with immunohistochemical methods. Results Histopathologic changes in varying degrees were demonstrated in the kidneys from animals of group B,C and D as compared with those from rats of group A. These changes were most pronounced in the kidneys from rats of group B while those in kidneys from rats of group C and group D were more trifling. The results of immunohistochemical analysis revealed that, the expression of NF-κB in kidneys from rats of group D[the percentage of positive cells was (19.58±4.94)%] was lower than that in the kidneys from rats of group B[(42.17±8.52)%](P<0.01). The expression of iNOS in the kidneys from rats of group D [the value of absorbance was(0.167 3±0.012 2)] was significantly lower than that in the kidneys from rats of group B[(0.325 4±0.027 6)](P<0.05). Conclusion The sustained activation of NFκB and iNOS along with the progress of pathological changes in the diabetic kidney may play an important role in the development of diabetic nephropathy and glurenorm may exert protective effects on the kidney by inhibiting the expression of NF-κB and iNOS.
  • 神经科用药专栏
  • 神经科用药专栏
    WANG Yuanfang;LI Zucheng;CHEN Da;YANG Zhihong;YUE Wang
    2007, 26(2): 143-146.
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    ABSTRACT Objective To compare the effects of different drugs on a model of chronic epilepsy kindled by electric stimulus of the amygdala in rats and the possible underlying mechanisms. MethodsA model of chronic epilepsy kindled by stimulation of the right amygdala with constant electric current was set up successfully in the male Wistar rat. The effects of different kinds of calcium antagonists on the after discharge duration(ADD) and Racine’s staging of the model kindled by electric stimulation of the amygdala were kept under observation and an autocontrol of the data before and after the medication was carried out. Results The effects of different calcium antagonists on the model were different. 40~80 mg·kg-1 L-type calcium antagonist nimodipine administered by gastrogavage inhibited epilepic seizures kindled by electric stimulation of the amygdala and lowered the Racine’s staging (P<0.01). 200 mg·kg-1 of the L-type calcium antagonist diltiazem administered by gastrogavage inhibited the ADD (P<0.05) while showing no effect on Racine’s staging(P>0.05). 100~250 mg·kg-1 of the T-type calcium antagonist ethosuximide administered by gastrogavage inhibited the ADD(P<0.05)but had no effect on the epileptic seizures and Racine’s staging(P>0.05). 10~20 mg of the Ttype calcium antagonist phenytoin sodium injected hypodermically inhibited the epileptic seizures and lowered the Racine’s staging (P<0.01). 500 mg of the T-type calcium antagonist sodium valproate administered by gastrogavage inhibited the epileptic seizures and lowered the Racine’s staging (P<0.05). 20~60 mg·kg-1 of the nonselective calcium antagonist flunarizine administered by gastrogavage inhibited the epileptic seizures and lowered the Racine’s staging(P<0.01) in a dosedependent manner. Conclusion①L-type and T-type calcium channels may be involved in the pathogenesis of epilepsy in the present study.②Nimodipine inhibited the epileptic seizures kindled by electric stimulation of the amygdala and relatively large doses of diltiazem inhibited the ADD probably by blocking the Ltype calcium channel.③Ethosuximide, known to block the T-type calcium channels in the thalamic neurons, was shown to exert no inhibition on the epileptic seizures kindled by electric stimulation of the amygdala, suggesting that the epileptic seizures kindled by electric stimulation of the amygdala or even grandmal epilepsy have nothing to do with the lowthreshold T-type calcium channel in the thalamus. ④Both phenytoin sodium and sodium valproate inhibited the epileptic seizures kindled by electric stimulation of the amygdala in this study. The Ttype calcium channel on which phenytoin sodium acted may be different from that on which ethosuximide did. These 2 drugs may also exert antiepileptic effects via other mechanisms. Sodium valproate, the drug usually used in the treatment of grandmal epilepsy or absence seizure, may be provided with calciumchannel blocking effects of both ethosuximide and phenytoin. ⑤The antiepileptic effects of flunarizine may be attributed to their blocking actions on various types of calcium channels.
  • 神经科用药专栏
    CHEN Jige;WU Hua
    2007, 26(2): 147-149.
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    ABSTRACT Objective To survey the effect of edaravone on the nervous function in rats with a model of acute spinal cord injury. Methods A model of moderate acute injury of the spinal cord was set up in each of the 32 SD rats according to the method described by Allen. The rats were then randomly divided into two equal groups: the trial group and control group. Rats of the trial group were given each an intraperitoneal injection of 3 mg·kg-1 of edavorine diluted with 0.9% sodium chloride solution q.12 h. Rats of the control group were given each 0.9% sodium chloride solution in the same manner as described. The course of treatment in both groups lasted 14 days. The results of the inclined plane technique and Torlov scale scoring in rats of groups 1, 2, 3, 4 and 6 weeks after the beginning of the experiment were compared. ResultsBeginning from the 2nd week after the injury, the angle of inclination of the plane in the test in rats of the trial group was significantly greater than that in rats of the control group (P<0.01) and the restoration rate of nervous function in rats of the trial group was also significantly greater than that in rats of the control group (P<0.01). Conclusion Edaravone was shown to promote the restoration of nervous function in rats with a model of acute spinal cord injury.
  • 神经科用药专栏
    CHEN Ji;CHENG Yong;QU Zhiwei;HUANG Shan;ZHANG Juntian
    2007, 26(2): 149-152.
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    ABSTRACT Objective To compare the effects of neurotropin and enzaishi in combating brain ischemia in rats and relieving pain caused by intraperitonial injection of acetic acid in mice. Methods(1) Male Wistar rats were randomly divided into 6 equal goups:① model group, ②and③neurotropin treatment groups, ④ and ⑤ enzaishi treatment groups, ⑥sham operation group. A model of focal cerebral ischemiareperfusion injury was set up in each of the animals in groups ① to ⑤. Rats of group ⑥ underwent a sham operation each. Rats of group ② and ③ were given each an IV injection of 10 and 20 U·kg-1of neurotropin, respectively. Rats of groups ④ and ⑤were given each an IV injection of 10 and 20 U·kg-1 of enzaishi, respectively. Rats of groups ① and ⑥ were given no treatment. The behavioral scorings, areas of brain infarction and brain water content in rats of the different groups were used as indexes for the assessment of the effects of neurotropin and enzaishi. (2) 175 mice of the Kunming strain were randomly divided into 7 equal groups: ① model,②low,③medium④highdose neurotropin,⑤low,⑥medium,⑦highdose enzaishi. Mice of group ① were given each an intraperitoneal(IP) injection of an equivalent amount of 0.9% sodium chloride solution. Mice of groups ②③ and ④ were given each an IP injection of 10,20 and 40 U·kg-1 of neurotropin, respectively. Mice of groups ⑤⑥ and ⑦ were given each an IP injection of 10,20 and 40 U·kg-1 of enzaishi, respectively. 0.5 h after the injection, mice of all 7 groups were given each an IP injection of 0.2 mL of 0.6% acetic acid. The frequencies of writhing reflexes exhibited by the animals within 15 min after the injection of acetic acid were registered and used to assess the analgesic effects of the drugs. Results(1)Neurotropin in doses of 10 and 20 U·kg-1 was shown to ameliorate the nervous function, reduce cerebral infarction area and mitigate brain edema in the rats. enzaishi in a dose of 20 U·kg-1 could decrease the brain water content and reduce cerebral infarction area. However, it could not reduce the cerebral infarction area at a dose of 10 U·kg-1. The nervous function was not improved by enzashi in both low and high doses. (2) Neurotropin in doses of 20 and 40 U·kg-1 was shown to strikingly inhibit the writhing reflex in mice after an IP injection of acetic acid, the difference between the frequencies of writhing reflexes in mice of the model group and treated group being significant(P<0.05). enzaishi in the same doses, however, had no effect on the writhing reflex. Conclusion Neurotropin in low and medium doses was shown to mitigate the brain injury caused by ischemiareperfusion in rats. enzaishi, however, could combat brain ischemia only at the medium dose. Neurotropin in medium and high doses was shown to exert a striking analgesic effect in mice given IP injection of acetic acid, while enzaishi in the same doses had no such effect.
  • 神经科用药专栏
    ZHOU Qingshan;XIA Wenfang;XIE Xiaoli;LIU Xianyi;DU Daping
    2007, 26(2): 152-155.
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    ABSTRACTObjectiveTo probe into the protective effects of murine nerve growth factor(NGF) on the spinal cord in the rat with a model of neuropathic pain. MethodsSixty-six adult male Wistar rats were randomly divided into 3 groups:①NGF group(n=30), ② saline group (n=30) and ③ sham operation group (n=6). A model of neuropathic pain was set up in each of the rats of group ① and group ② by ligating the left sciatic nerve under chloral hydrate anesthesia. The sciatic nerve of each of the rats of group ③ was exposed but not ligated. The rats of group ① and group ② were given each an intraperitoneal injection of 20 μg·kg-1 of murine NGF and an equivalent amount of 0.9% sodium chloride solution after the operation, respectively. Behavioural testings including the times of paw withdrawal and tail flicking in animals of all 3 groups were carried out 1 day before the operation, as well as 6 hour, 1, 3, 7 and 14 days after the surgery. Expressions of TNFα and IL6 in the spinal cord were assayed with the immunohistochemical methods and image analysis. Apoptosis of the spinal cord neurons was detected with the TUNEL method and the histopathological changes of the spinal cord were examined with light microcopy under HE and Nissl’s stainings . ResultsThe 50%paw withdrawal threshold in rats of the NGF group was significantly higher than that in rats of the saline group from the 3rd day after the operation to the termination of the experiment (P<0.01). From the 1st day after the operation to the termination of the experiment, the hot water flick tail threshold was strikingly higher in rats of the NGF group than that in those of the saline group(P<0.05, P<0.01). The expression of TNFα and IL6 in the spinal cord as well as the spinal cord neuronal apoptotic index in rats of the NGF group were significantly lower than those in rats of the saline group(P<0.01). Histopathological examination demonstrated that the injury of the spiral cord tissue was much more trifling in rats of the NGF group than that in those of the saline group. ConclusionMurine NGF was shown to exert protective effects on the spinal cord in the rats with a model of neuropathic pain possibly by inhibiting the expression of proinflammatory cytokines and thereb mitigating neuronal apoptosis and sensation of pain.
  • 神经科用药专栏
    WANG Dabin;MING Meng;QI Xusheng;WANG Yong;LIU Xinmin
    2007, 26(2): 156-156.
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    ObjectiveTo study the curative effect and safeness of topiramate in the treatment of refractory partialonset epilepsy in children. Methods36 patients with childhood refractory partialonset epilepsy were enrolled in the study. The kinds and dosages of antiepileptics formerly used by the patients remained unchanged in the present study. Topiramate was then used as an additive remedy, beginning with a dose of 0.5 mg·kg-1·d-1 per os. The dose was increased by 0.5 mg·kg-1·d-1 every week, aiming at a final dose of 4.0~9.0 mg·Kg-1·d-1. When, however, obvious curative effects appeared, the gradual increase in the dosage of the drug was stopped and the dosage on that very day was taken as the maintenance dose thereafter. The antiepileptics formerly used by the patients were given in gradually decreasing doses followed by discontinuation of the drugs after the epilepsy had been controlled. ResultsThe overall effective rate in the 36 patients after 6 months of treatment was 78.0%. In comparison with the status before the treatment, the total seizure frequency was decreased by 73.0%(P<0.01). Among these, the simple partial seizure (SPS) frequency was decreased by 79.0%(P<0.05), complex partial seizure (CPS) frequency was decreased by 71.0%(P<0.05), and second generalized seizure SGS seizure frequency was decreased by 67.0%(P<0.01). At the termination of the 6month treatment, no overt anomalies were demonstrated by ECG, liver and kidney function tests and routine examinations of the blood and urine. Mild to moderate adverse reactions were encountered in 11 patients when the doses of topiramate were steadily increased. These reactions usually disappeared spontaneously demanding no particular managements. ConclusionTopiramate was shown to exert definite curative effects in the treatment of refractory partialonset epilepsy, in children. It was also fairly safe and welltolerated by the patients.
  • 药物与临床
  • 药物与临床
    ZHOU Hui;WANG Zhen;DU Shiming;HUANG Liangyong
    2007, 26(2): 166-167.
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    ABSTRACT Objective To survey the curative effect of compound levofloxacin spray in the treatment of infective skin diseases caused by bacteria and viruses. MethodsOne hundred patients with infective skin diseases caused by bacteria were randomly divided into two groups: the trial group A (n=52) and control group A (n=48). One hundred and four patients with infective skin diseases caused by viruses were randomly divided into two groups: the trial group B (n=56) and control group B (n=48). Patients of the trial groups A and B were treated q.i.d. with topical spraying of levofloxacin until the affected area was moistened. Patients of the control groups A and B were treated with smearing of the affected areas with erythromycin ointment and aciclovir ointment q.i.d., respectively. The course of treatment in the four groups of patients lasted 3~5 d. The curative effects in patients of the different groups were compared. ResultsThe cure rate, rate of excellent therapeutic effect, effective rate and ineffective rate in patients of the trial group A were 42,8,2 and 0, respectively, the overall effective rate being 100.00%. The corresponding rates in patients of the control group A were 25,13,2 and 8, respectively, the overall effective rate being 83.33%. The overall effective rate in patients of the trial group A was significantly higher than that in patients of the control group A (P<0.01). The cure rate, the rate of excellent therapeutic effectiveness, the effective rate and ineffective rate in patients of the trial group B were 38,8,10 and 0, respectively, the overall effective rate being 100.00%. The corresponding rates in patients of control group B were 18,12,11 and 7, respectively, the overall effective rate being 85.42%, which was significantly lower than that in patients of trial group B (P<0.01). ConclusionCompound levofloxacin spray was shown to be fairly effective in the treatment of infective skin diseases caused by bacteria and viruses.
  • 药物与临床
    LI Zhou;ZHU Guijin;JIN Lei;ZHANG Hanwang
    2007, 26(2): 168-170.
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    ABSTRACTObjectiveTo study the effect of oral contraceptive pills (OCP) administered before the stimulation cycle on poor responder patients undergoing in vitro fertilizationembryo transfer (IVF-ET) cycles. Methods143 poor responder patients undergoing IVF-ET cycles were divided into 2 groups according to the history whether the patients had been treated with OCP before the ovulatory period (trial group, n=69) or not treated with the drug (control group, n=74). A statistical analysis of the anamnestic data of the patients was carried out for the assessment of the ovulationpromoting effect of OCP and their influence on the IVF-ET operation. ResultsThe required dosis of gonadotrophin was lower in patients pretreated with OCP [( 51.37±13.67) amps ]than that in patients of the control group [(44.06±9.30)amps](P<0.05=. The cycle cancel rate in patients of the trial group (11.6%) was also lower than that in patients of the control group (25.7% ) (P<0.05). The differences between patients of the 2 groups with respect to number of oocyte retrieval , fertilization rate and clinical pregnancy rate, however, were insignificant. ConclusionOCP administered before IVF-ET were shown to improve the effect of ovulationpromotive treatment and decrease the cycle cancel rate in poor responder patients
  • 药物制剂
  • 药物制剂
    WEI Fengling;WU Sheng;CUI Gang
    2007, 26(2): 182-184.
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    ABSTRACTObjectiveTo study the effect of β-cyclodextrin inclusion on the transdermal absorption of borneol in vitro. MethodsDepilated skin segments from NIH mice were smeared with emulsion of borneol β-cyclodextrin inclusion and conventional emulsion of borneol separately. The transdermal absorption of borneol was assayed in a Franz diffusion cell using thinlayer chromatography to determine the penetration rates of borneol at different time paints. ResultsA striking decrease in the rate of transdermal penetration of borneol that was included by βcyclodextrin was demonstrated. The borneolβ-cyclodextrin inclusion is provided with the property of a sustainedrelease drug. It prevents the volatilization of borneol and promotes the stability of the drug. ConclusionBorneolβ-cyclodextrin inclusion can be transdermally absorbed in a sustainedrelease manner.
  • 药品质量控制
  • 药品质量控制
    ZHANG Yonghui;ZHANG Jinwen;RUAN Hanli;PI Huifang;JIA Yu;WU Jizhou
    2007, 26(2): 187-189.
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    ABSTRACTObjectiveTo set up an HPLC fingerprint of the total saponins in shenqifuzheng injection. MethodThe devices used were Waters 717 high performance liquid chromatograph, Waters 2996 diode array detector, low pressure quaternionic pump, autosampler and KR1005C18 chromatographic column (250 mm×4.6 mm,5 μm). The mobile phase was acetonitrilewater, using linear gradient elution, the column temperature was 30 ℃; flow rate, 0.8 mL·min1; detection wavelength, 270 nm; proceeding time, 90 minutes. ResultsThe fingerprints of all the 10 batches of samples showed a greater than 90% similarity to those of total saponins in the control shenqifuzheng injection. The RSD of the relative retention time as well as that of the relative peak area of the shared peak was lower than 3% in the precision, stability and reproducibility tests.ConclusionThe method was shown to be handy and reliable and may be used in the quality control of the total saponins in shenqifuzheng injection.