神经科用药专栏
CHEN Ji;CHENG Yong;QU Zhiwei;HUANG Shan;ZHANG Juntian
2007, 26(2): 149-152.
ABSTRACT Objective To compare the effects of neurotropin and enzaishi in combating brain ischemia in rats and relieving pain caused by intraperitonial injection of acetic acid in mice. Methods(1) Male Wistar rats were randomly divided into 6 equal goups:① model group, ②and③neurotropin treatment groups, ④ and ⑤ enzaishi treatment groups, ⑥sham operation group. A model of focal cerebral ischemiareperfusion injury was set up in each of the animals in groups ① to ⑤. Rats of group ⑥ underwent a sham operation each. Rats of group ② and ③ were given each an IV injection of 10 and 20 U·kg-1of neurotropin, respectively. Rats of groups ④ and ⑤were given each an IV injection of 10 and 20 U·kg-1 of enzaishi, respectively. Rats of groups ① and ⑥ were given no treatment. The behavioral scorings, areas of brain infarction and brain water content in rats of the different groups were used as indexes for the assessment of the effects of neurotropin and enzaishi. (2) 175 mice of the Kunming strain were randomly divided into 7 equal groups: ① model,②low,③medium④highdose neurotropin,⑤low,⑥medium,⑦highdose enzaishi. Mice of group ① were given each an intraperitoneal(IP) injection of an equivalent amount of 0.9% sodium chloride solution. Mice of groups ②③ and ④ were given each an IP injection of 10,20 and 40 U·kg-1 of neurotropin, respectively. Mice of groups ⑤⑥ and ⑦ were given each an IP injection of 10,20 and 40 U·kg-1 of enzaishi, respectively. 0.5 h after the injection, mice of all 7 groups were given each an IP injection of 0.2 mL of 0.6% acetic acid. The frequencies of writhing reflexes exhibited by the animals within 15 min after the injection of acetic acid were registered and used to assess the analgesic effects of the drugs. Results(1)Neurotropin in doses of 10 and 20 U·kg-1 was shown to ameliorate the nervous function, reduce cerebral infarction area and mitigate brain edema in the rats. enzaishi in a dose of 20 U·kg-1 could decrease the brain water content and reduce cerebral infarction area. However, it could not reduce the cerebral infarction area at a dose of 10 U·kg-1. The nervous function was not improved by enzashi in both low and high doses. (2) Neurotropin in doses of 20 and 40 U·kg-1 was shown to strikingly inhibit the writhing reflex in mice after an IP injection of acetic acid, the difference between the frequencies of writhing reflexes in mice of the model group and treated group being significant(P<0.05). enzaishi in the same doses, however, had no effect on the writhing reflex. Conclusion Neurotropin in low and medium doses was shown to mitigate the brain injury caused by ischemiareperfusion in rats. enzaishi, however, could combat brain ischemia only at the medium dose. Neurotropin in medium and high doses was shown to exert a striking analgesic effect in mice given IP injection of acetic acid, while enzaishi in the same doses had no such effect.