中国科技论文统计源期刊 中文核心期刊  
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊  
日本科学技术振兴机构数据库(JST)
第七届湖北十大名刊提名奖  

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    药物与临床
  • 药物与临床
    ZHU Peiling;ZHU Peijun;CHEN Zhien;ZHANG Jing;ZHAN Xuemei;LI Qiongzhi
    2006, 25(6): 0-0.
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    ObjectiveTo appraise the therapeutic effectiveness and safety rate of aripiprazole in the treatment of schizophrenia. Methods80 patients with schizophrenia were randomly divided into two equal groups: the trial group and control group. Patients of these 2 groups were given each 10-20 mg·d-1 of aripiprazole PO,q.d. and 300-500 mg·d-1 of clozapine, PO, b.i.d, respectively. The course of treatment in both groups lasted 8 weeks. The therapeutic effectiveness was assessed with the Positive and Negative Syndrome Scale (PANSS), while adverse reactions were appraised with the Treatment Emergent Symptom Scale (TESS). ResultsThe reduction rate of PANSS total scores and the effective rate in patients of the trial group were 50.4% and the 87.5%, respectively, while those in patients of the control group were 49.4% and 85.0%, respectively. The overall curative effects were similar in patients of the two groups (P>0.05).Aripiprazole started earlier to act upon negative symptoms as compared with clozapine. The incidences of adverse reactions including oversedation, salivation, constipation, weight gaining etc. in patients of the trial group were significantly lower than those in patients of the control group (P<0.05 or P<0.01). ConclusionAripiprazole was shown to be safe and effective in the treatment of schizophrenia.
  • 药物制剂
  • 药物制剂
    WANG Wei;WANG Zhichao;DING Zhimin;LIU Zuxiong
    2006, 25(6): 0-0.
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    ObjectiveTo prepare sulindac chitosan gel and apply the drug to the clinic. MethodsAdd 5.0 g of sulindac and 50.0 g of glycerin to 500 mL of methanol with stirring for dissolution. Add 400 mL of pure water to the solution for dilution. Cast 10.0 g of chitosan and 5.0 g of carbomer on the surface of the liqid for fullblown swelling followed by the addition of 5.0 g of laurocapram and triethanolarnine qs with strring. Add pure water to 1000 mL with stirring and the gel was completed. Ultraviolet spectrophotometry was used to determine the content of sulindae in the gel. 135 patients with rheumatoid arthritis (RA) were randomly divided into 2 groups: the trial group (n=75) and control group (n=60). The affected joints in patients of the trial group were smeared with 0.1-0.5 g of the sulindal chitosan gel per joint according to the size of the joints t.i.d. . Patients of the control group were given each 0.2 g of sulindac PO,b.i.d.. The course of treatment in both groups lasted 8 weeks. ResultsThe average rate of recovery in content determination of sulindac was 100.3% with an RSD of 1.8%(n=5). The overall effective rates in patients of the trial group and control group were 93.3% and 81.7%, respectively (P<0.05). ConclusionsThe quality of the preparation accords with the relevant regulations of gel preparation of the Chinese Pharmacopoeia. It may thus be used in the clinic.
  • 用药指南
  • 用药指南
    WANG Qibin;ZHANG Penghua;XIAO Miaosheng;TU Ziliang;CHEN Shixi;DI Wei;MA Zuwen;YU Xueying;WANG Dan
    2006, 25(6): 0-0.
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    ObjectiveTo study the stability of kudiezi injection in 5% glucose solution, 10% glucose solution, 0.9% sodium chloride solution, 5% glucose in sodium chloride solution and compound sodium chloride solution. MethodsChanges in the outward appearance, pH value ,number of particles and UV absorption spectrum of the mixed solutions were kept under observation after the kudiezi injection had been added to the five different solutions. ResultsNo apparent changes were shown in the outward appearance and UVVis λ max of the 5 mixed solutions. There was a trend of increase in the number of particles along with time. A striking increase in the pH value was detected after the mixing of the kudiezi injection with compound sodium chloride solution. ConclusionKudiezi injection was shown to be not compatible with compound sodium chloride solution.
  • 药品质量控制
  • 药品质量控制
    ZHU Xiaojun;CHEN Bin
    2006, 25(6): 0-0.
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    ObjectiveTo set up a new method for the determination of the content of tetrahydropalmatine in shugan pills with HPLC.MethodsThe chromatographic conditions: A Spherisorb C18(4.6 mm×250 mm,5 μm) served as the chromatographic column; methanolwater (75∶25, the pH value adjusted to 8 with 0.05 mol·L-1 Na2HPO4 solution) was the mobile phase, the mixture was filtered through a Millipore membrane filter with an average pore diameter of 0.45 μm; UV detection wavelength, 280 nm; column temperature, 25 ℃; flow rate, 0. 9 mL·min-1. ResultsThere was a good linear relationship between the corresponding peak areas and tetrahydropalmatine when its concentrations were in the range of 1.76-21.12 mg·L-1 ( r=0. 999 8). The average rate of recovery was 98.6% with an RSD of 1.38%. ConclusionThe method was shown to be handy, accurate and reproducible and did not interfere with other medicinal materials in the prescription. It may thus be used as one of the methods for the content determination of tetrahydropalmitine in shugan pills.
  • 药物制剂
  • 药物制剂
    LIU Shi;XU Zhe;LUO Zhi;XIANG Guangya
    2006, 25(6): 0-0.
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    ObjectiveTo prepare and characterize folatecoupled chitosan nanoparticles. MethodsOptimal technological conditions were selected according to the coupling ratio of folate and chitosan. Folatecoupled chitosan was prepared by the reaction of the activated folate ester with the amine group on the chitosan. Folatecoupled chitosan nanoparticles were then prepared with the ionic crosslinking method and the average diameter, Zeta potential of the nanoparticles were determined. ResultsIt was shown by the results of an orthogonal test that the amount of the activated folate ester used and the reaction temparature were the main factors influencing the coupling ratio. Folatecoupled chitosan with a ratio of about three folate molecules coupled to one chitosan molecule could be prepared when the ratio of the amounts of the active folate ester to chitosan used was 2∶1, the reaction temperature was 50 ℃ and the reaction time was 2 h. The average diameter and Zeta potential of the nanoparticles were 316 nm and (24.85±1.14) mv, respectively. The folatecoupled chitosan nanoparticles were spherical in shape as shown under the transmission election microscope. ConclusionFolatecoupled chitosan nanoparticles could be prepared successfully with the method.
  • 药物研究
  • 药物研究
    LIU Weizhong;HUANG Weiqiao;WANG Guangfa;FUYan;WEN Yuguan
    2006, 25(6): 0-0.
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    ObjectiveTo set up an HPLC method for the determination of the concentration of the metabolite of oxcarbazepine in human blood plasma. MethodsBlood plasma samples containing the drug were extracted with hexane for the removal of lipids and other interfering substances and thereafter extracted with ethyl acetate. The extract was then analyzed in a reverse phase HPLC system(C18 column,150 mm×4.6 mm, 5 μm); mobile phase,methanolwater(55∶45);UV detection wavelength,254 nm). ResultsThe average rates of recovery of the metabolite of oxcarbazepine in high , medium and low concentrations(120.0,60.0,12.0 mg·kg1)were 100.43%,99.33% and 99.17%,respectively. The interday and intraday relative standard deviations were less than 6%(n=5). The calibration curve had a good linearity r=0.999 6(n=10) when the metabolite was within a concentration range of 12.0-120.0 mg·L-1. The regression equation was C=77.06F+1.22,r=0.999 6(n=10). The minumum detectable concentration of the metabolite of oxcarbazepine was 6.0 mg·L-1. ConclusionThe method was shown to be sensitive,accurate,handy and speedy and may be used in the monitoring of the blood concentration of oxcarbazepine and phamacokinetic studies of the drug.
  • 中药与天然药物专栏
  • 中药与天然药物专栏
    ZHANG Dong qi;SUN Lisha;XU Jiangping
    2006, 25(6): 0-0.
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    ObjectiveTo survey the effect of piceid on dogs with a model of acute myocardial infarction. Methods30 healthy mongrel dogs weighing 10-15 kg served as the subjects of the study. The model of acute myocardial infarction was established by ligation of the left anterior descending branch of the coronary artery in the dogs under anaesthesia and mechanical ventilation. 10 minutes after the ligation the dogs were given each piceid in low (2.5 mg·kg-1,n=6), medium (5.0 mg·kg-1,n=6) and high(10.0 mg·kg-1, n=6) doses, respectively, administered by IV instillation lasting 30 min.Animals of the solvent control group (n=6) were given each an equivalent volume of the solvent in the same manner while those of the blank control group (n=6) were given no treatment. The severity of myocardial ischemia was reflected by the epicardial electrocardiogram. Blood samples from the right ventricle were taken before and 2 h after the medication for the determination of the activities of the serum lactate dehydrogenase(LDH) and creatine kinase (CK). 2 h after the ligation of the artery, the dogs were sacrificed and the hearts taken for the determination of the extent of infarction with the NBT staining method. ResultsIntravenous instillation of piceid was shown to significantly decrease the severity of myocardial ischemia and reduce the extent of infarction in a dosedependent manner. The drug was also found to mitigate the increase in the activities of serum LDH and CK. ConclusionPiceid injection was shown to have striking protective effects on dogs with acute experimental myocardial infarction.
  • 中药与天然药物专栏
    SHAO Ziqiang;JIAO Jingsong;XUE Shuang;WANG Guoxiang
    2006, 25(6): 0-0.
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    ObjectiveTo study the clinical effect of puerarin injection in the treatment of patients with cerebral infarction. Methods7 600 inpatients with cerebral infarction serving as the subjects of the study were subjected to basic medical treatment including antiplatelet drugs, dehydration, antiinfectives, control of blood pressure, blood glucose, blood lipids, etc.. Of these, 2 500 patients were given each 500 mL of puerarin injection q.d., 2 000 patients were given each 400 mL of red sage root injection q.d., another 2 000 patients were given each 450 mL of total notoginsenoside injection q.d.. All these drugs were administered by intravenous instillation. The remaining 1 100 patients serving as controls were not given additional special treatment. The course of treatment in all these patients lasted 21 days. The effective rate, extent of nervous function improvement,case fatality rate and incidence of postinfarction cerebral hemorrhage(CH) in patients of the different groups were compared. ResultsThe overall effective rates in patients treated with puerarin, red sage root, total notoginsenoside and those serving as controls were 79.20%,78.50%,60.15% and 36.00%, respectively, the difference between the effective rates of the puerarin group and notoginsenoside group being significant(P<0.05)and that between the puerarin group and control group, highly significant(P<0.01). The extent of nervous function improvement in patients of the puerarin group was significantly greater than that of red sage root group(P<0.01) and control group (P<0.01) but similar to that of the notoginsenoside group(P>0.05). The case fatality rate in patients of the puerarin group(1.40%) was significantly lower than those of the notoginsenoside group(2.40%,P<0.05), red sage root group (3.60%,P<0.01) and control group (8.18%,P<0.01). The incidence of CH in patients of the puerarin group (2.40%) was higher than that of the control group (1.10%,P<0.01), lower than that of the notoginsenoside group(5.00%,P<0.05) and similar to that of the red sage group (2.55%,P>0.05). ConclusionPuerarin injection was shown to strikingly improve the nervous function and reduce case fatality rate in the treatment of patients with cerebral infarction.
  • 药物与临床
  • 药物与临床
    LUO Huijuan;LI Weijing
    2006, 25(6): 0-0.
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    ObjectiveTo survey the effect of peroral flucloxacillin in the prevention of infection after cesarean section. Methods566 predelivery women scheduled for cesarean section were divided into 2 groups: the trial group (n=301 ) and control group(n=265). Women of the trial group were given each an IV injection of 1.0 g of rocephin 30 min before the operation and 0.5 g of flucloxacillin capsules PO,b.i.d. after the operation for 5 consecutive days . Women of the control group were given each an IV injection of 1.0 g of rocephin after the operation for 3 consecutive days. Other managements for the parturients of the 2 groups during the perinatal period were similar. The incidence of puerperal infection in the 2 groups was compared. Results34 and 19 cases of puerperal infection were diagnosed in women of the trial group and control group, respectively, the difference being insignificant (P>0.05). No adverse reactions of the drugs were encountered in the 2 groups. ConclusionPeroral flucloxacillin may be used in parturients after aseptic cesarean section for the prevention of infection and harmful effects of systemic medication on the mother and child as well as for the reduction of medical expense.
  • 药物研究
  • 药物研究
    ZHANG Chengliang;CAI Xiaohan;LI Qiu;PENG Jiabei;XIANG Ming
    2006, 25(6): 0-0.
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    ObjectiveTo probe into the method and mechanism of the prevention and treatment of IDDM in mice by induction of immune tolerance with the autoantigen insulin. Methods32 male BALB/c mice 8 weeks of age were randomly divided into 4 equal groups: blank control, model control, insulin s.c. (subcutaneous injection) and insulin gg (gastrogavage). The IDDM model was established in mice of the 4 groups by a daily intraperitoneal injection of 40 mg·kg-1 of streptozotocin(STZ) per mouse for 5 consecutive days. Mice of the insulin s.c. group were given each a subcutaneous injection of 100 μg of bovine insulin one day before the establishment of the IDDM model and once weekly thereafter for 4 consecutive weeks. Animals of the insulin gg group were given each 150 μg of bovine insulin administered by gastrogavage one day before the establishment of the IDDM and the same treatment was thereafter repeated twice weekly for 4 consecutive weeks. Blood glucose was then determined weekly and the animals of all 4 groups were sacrificed 6 weeks later. Pancreas tissues were taken for histologic examination with HE staining and splenocytes harvested for the assay of lymphocyte proliferation with the MTT method. Besides, splenocytes were also subjected to the determination of the ratio of the CD+4 CD+25 T cell sulpopulations with FACS. ResultsAn IDDM model was successfully established in BALB/c mice by repeated intrapritoneal injection of low dose STZ as evidenced in mice of the model control group by beta cell destruction as a result of lymphocyte infiltration. The level of blood glucose in mice of the insulin s.c. group, but not that in animals of the insulin gg group, was significantly lower than that in mice of the model control group(P<0.05). By and large no lymphocyte infiltration in the islets of the pancreas and no obvious β cell destruction could be found in the pancreatic tissues from mice of the insulin s.c. group. A depressed lymphocyte proliferation and higher ratio of the CD+4 CD+25 T cell subpopulations were demonstrated in mice of the insulin s.c. group as compared with those in mice of the blank and model control groups(P<0.05). ConclusionSubcutaneous administration of bovine insulin in mice with IDDM was shown to induce immunotolerance resulting in protection against the disease, possibly by the upregulation of the CD+4CD+25 T cell subpopulations.
  • 药物研究
    HUANG Yaqin;CEN Yanyan;ZENG Benhua;LIU Yu;WEI Hong
    2006, 25(6): 0-0.
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    ObjectiveTo study the toxicokinetics of lovastatin (LV) in male Bamaminipigs. Methods4 healthy male Bamaminipigs were given each 1.25 g·kg-1 of LV administered by gastrogavage after a 12 h fasting. Venous blood samples were collected from each of the animals 0.5,1.0,1.5,2.0,2.5,3.0,4.0,6.0,9.0,12.0,24.0 and 30.0 h after the medication. RP-HPLC was used to determine the concentration of LV in the sera. The toxicokinetic parameters were computed with the 3P97 program. ResultsThe main toxicokinetic parameters of LV were as follows: distribution phase half- life t1/2(α)=0.807 h; elimination phase half life t1/2(β)=18.376 h; AUC t=0→∞=95.964 μg·h·mL-1. ConclusionThe toxicokinetic behaviour of LV in Bamaminipigs was shown to be accordant with the twocompartment extravascular medication model provided with the firstorder elimination. LV could be rapidly eliminated from the male Bamaminipigs.
  • 药物研究
    SUN Yanping;JIANG Cuie;PENG Shuhui
    2006, 25(6): 0-0.
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    ObjectiveTo probe into the possible protective effect of vitamin E on male rats submitted to testicular toxicity induced by doxorubicin. MethodsThe rat model of testicular toxicity was established by a single intravenous injection of 7.5 mg·kg-1 of doxorubicin hydrochloride for each animal. Beginning from the day before the establishment of the model, rats of the vitamin E group (n=8) were given each 100 mg·d-1 of vitamin E by gastrogavage for 14 consecutive days. Animals of the model group (n=8) were given each after the establishment of the model an introperutoneal injection of 1 mL of 0.9% sodium chloride solution q.d. for 14 days. Rats of the control group (n=8, not subjected to model establishment) were given each at the same time a daily intraperutioneal injection of 1 mL of 0.9% sodium chloride solution. The rats were sacrificed 4 weeks later. The testicular toxicity induced by adriamycin was assessed with the measurement of the activities of superoxide dismutase (SOD) and the content of malonyldialdehyde (MDA) in the serum as well as histopathological examination of the testes. ResultsIn comparison with animals of the control group, rats of the model group had lower SOD activities and higher MDA content in the serum (P<0.05) as well as a sharp decrease in the absolute and relative testicular weight (P<0.05) associated with a striking reduction of the seminiferous epithelial cells, while the spermatocytes and spermatids were degenerated and a portion of them had undergone necrosis and scaling. In contrast, the difference between rats of the vitamin E group and control group with respect to serum SOD activities and MDA contents was not significant(P> 0.05),and the changes in the weights of the testes as well as the lesions of seminiferous epithelial cells were trifling in rats of the VE group as compared with those in rats of the model group. ConclusionVitamin E was shown to exert protective effects on rats submitted to doxorubicininduced testicular toxicity possibly related to its capability of promoting the antioxidative activity and inhibiting the lipid peroxidation of the organism.
  • 药物研究
    WU Mao;SHANG Guomei;HUANG Shuimei;WANG Jianfu;LIU Jiandong;LIN Huijun
    2006, 25(6): 0-0.
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    ObjectiveTo appraise the effects of ribavirin, ganciclovir and vidarabine on the peripheral blood platelets and megakaryocytes of the bone marrow in rabbits. Methods20 rabbits were randomly divided into 4 equal groups. Animals of the groups A, B, C and D were given each a daily IV injection of 15 mg·kg-1 of ribavirin, 10 mg·kg-1of ganciclovir, 10 mg·kg1of vidarabine and an equivalent amount of 0.9% sodium chloride solution via the marginal vein of the ear, respectively. The course of treatment lasted 14 days. During the treatment course, samples of peripheral blood were collected every 4 days for platelet counting. 1 day as well as 8 days after the termination of the treatment, bone marrow was aspirated for the counting of megakaryoeytes. Results1 day after the termination of treatment, the numbers of the bone marrow megakocyocytes in rabbits of group A and group B were smaller than that in rabbits of group D (P<0.05), while the megakaryocyte number in animals of group C was even still smaller than that in animals of group D (P<0.01). 8 days after the termination of treatment, bone marrow megakarypocytes in rabbits of group A,B and C were shown to have undergone considerable degrees of restoration in number. A good correlation between megakaryocytes and platelets was demonstrated (r=0.999,P<0.05). During the course of treatment, the platelet countings showed a sharp increase at first followed by a gradual fall in rabbits of group A, a sustained decline in animals of group C and no impressive changes in rabbits of group B and group D. ConclusionDifferent antivirals were shown to have different effects on megakaryocytes and platelets in rabbits, suggesting that one should be prudent in the choice of drugs for patients with disorders of platelet and hemopoietic functions.
  • 药品质量控制
  • 药品质量控制
    ZHU Guanghui;WANG Qizhi;WANG Zengshou;SU Xiaogong
    2006, 25(6): 0-0.
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    ObjectiveTo set up a reversedphase HPLC method for the determination of aristolochic acid Ⅰand Ⅱ in ganluxiaodu pills. MethodsA C18 chromatographic column (4.6 mm×250 mm, 5 μm) served as the solid phase; methanolwateracetic(68∶32∶1) was the mobile phase; the flow rate, 1.0 mL·min-1 ; UV detection wavelength, 250 nm. The aristolochic acids Ⅰand Ⅱ in the ganluxiaodu pills were extractad with the ultrasonic extraction method. ResultsThere was a good linear relationship over a range of 0.135-27.000 μg ·mL-1 for aristolochic acid Ⅰ(r=0.999 8 ,n=5) and 0.325-65.000 μg·mL-1 for aristolochic acid Ⅱ(r=0.999 9, n=5). The lowest detection concentrations of aristolochic acid Ⅰ and Ⅱ were 0.081 μg·mL-1 and 0.065 μg·mL-1,respectively. The average rates of recovery of aristolochic acid Ⅰ and Ⅱ were 98.50% and 99.12%,respectively, the RSD were 2.30% and 3.50%,respectively. ConclusionThe method was shown to be handy, rapid and sensitive.