药物研究
REN Leiming;ZHU Zhongning;WANG Miao;WANG Lili;TIAN Helin;HE Dongwei
2006, 25(4): 0-0.
ObjectiveTo study the effects of urethane and pentobarbital sodium in anesthetizing doses administered intravenously or intraperitoneally on the blood glucose levels in mice and the underlying mechanisms. Methods①80 male Kunming mice were randomly divided into 8 equal groups. Mice of group 1 to group 3 were given each 1.0 g·kg-1 of urethane via the caudal vein. Blood samples were taken 15,30 and 60 min after the urethane injection for the determination of glucose concentration with the glucose oxidase method. Mice of group 4 ,serving as control, were given each an IV injection of equivalent amount of 0.9 % sodium chloride (NS) solution. Blood samples were taken as well for glucose determination as deseribed. Mice of group 5 to group 7 were given each an IV injection of 40 mg·kg-1 of pentobarbital solution. Mice of group 8, serving as control, were given each an IV injection of equivalent amount of 0.9 % NS. Blood samples were taken from these mice 15,30 and 60 min after the injection for blood glucose determination. Muscle and liver tissues were taken from all the mice of these 8 groups immediately after the collection of blood samples for the determination of glycogen with the anthrone method. ②80 male mice were randomly divided as described in experiment ①. Except for mice of the control groups, these animals were given each an intraperitoneal injection of 1.0 g·kg.-1 of urethane or 40 mg·kg-1 of pentobarbital solution. Blood samples were taken for glucose determination as described in experiment ①.③ 20 mice were randomly divided into 2 equal groups, mice of the trial group were given each 1.0 g·kg-1 of urethane IV and those of the control group were given each equivalent amount of NS IV. Blood samples were taken 30 min after the injections for the counting of red blood cells, white blood cells and blood platelets. ResultsBlood glucose concentration was increased by 48.4 % 15 min after the intravenous administration of urethane at an anesthetizing dose. In contrast, blood glucose concentration was increased by 204 % and 59.3 % 15 min and 60 min, respectively, after the intraperitoneal injection of urethane at that same anesthetizing dose. Intravenous or intraperitoneal administration of pentobarbital sodium at anesthetizing doses did not bring about apparent changes in the blood glucose concentration of the animals. The glycogen content of the liver was significantly increased after the IV injection of urethane or pentobarbital sodium, while the content of muscle glycogen was not apparently affected. Intravenous administration of urethane did not change the countings of red blood cells, white blood cells or blood platelets significantly as compared with results of the controls. ConclusionIntraperitoneal administration of urethane was shown to induce a hyperglycemia far more pronounced than that caused by intravenous administration of the drug at the same dose, suggesting that a local stimulating effect exerted by urethane may aggravate the hyperglycemia in mice.
