中国科技论文统计源期刊　中文核心期刊  
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《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊  
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第七届湖北十大名刊提名奖  

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    用药指南
  • 用药指南
    he Therapeutic Effectiveness and Pharmacoeconomics of Compound Vitamin B12 and rhEGF in the Treatment of Oral Mucositis Caused by Radiotherapy in Patients with Nasopharyngeal Carcinoma
    ZHENG Ying;WANG Yiru;ZHANG Yang;LIN Saie;ZHU Zhiwei
    2005, 24(8): 0-0.
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    ObjectiveTo study the therapeutic effectiveness and effectcost ratio of compound Vitamin B12 and recombinant human epidermal growth factor（rhEGF） in the treatment of oral mucositis caused by radiotherapy in patients with nasopharyngeal carcinoma（NPC）.Methods80 cases of NPC scheduled for radiotherapy were randomly divided into 2 equal groups, group Ⅰand group Ⅱ .Patients of both groups were submitted to linear acceleration or 60Co radiotherapy. The dose of the radiotherapy was 2 Gy per time, 5 times a week. The course of treatment lasted 6 weeks. Oral mucositis began to appear in patients in the 2 nd week of radiotherapy. Beginning from this week, patients of group Ⅰand group Ⅱ were given each 2 mL of compound vitamin B12 ,t.i.d. and 1 mL of rhEGF b.i.d. or t.i.d., respectively, administered by oral spraying. This treatment persisted until the termination of radiotherapy. Adverse reactions ,costs and therapeutic effects of the 2 drugs were registered for analysis..ResultsThe effective rates of compound vitamin B12 and rhEGF in the treatment of oral mucositis in patients of groupⅠand groupⅡwere 87.5% and 92.5% ，respectively, the difference being insignificant (P＞0.05). However, the cost of compound vitamin B12 (296.00 yuan) was significantly lower than that of rhECF (583.00 yuan).ConclusionFrom the viewpoint of pharmacoeconomics ,it seems preferable to use compound vitamin B12 with a higher effect/cost ratio in the treatment of oral mucositis caused by radiotherapy in patients with NPC.
    • 药物研究
  • 药物研究
    LD50 Determination of Peiminine and Its Five Derivatives in Mice
    YANG Xiliang;ZHANG Yonghui;RUAN Hanli;PI Huifang;WU Jizhou
    2005, 24(8): 0-0.
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    ObjectiveTo determine the acute toxicity and LD50 of peiminine and its five derivatives in mice. MethodsMice of the Kunming strain were randomly divided into 33 groups with 10 animals in each group. Mice of different groups were given each an intraperitoneal injection of peiminine and its 5 derivatives in different doses separately.Values of LD50 and 95% confidence interval of the 6 drugs were then calculated with the Bliss method. ResultsThe LD50 of acetyl peiminine, propionyl peiminine, butyryl peiminine, benzoyl peiminine and peiminine oxide were shown to be 14.39, 14.47, 18.41, 23.21 and 9.98 mg·kg-1 ,respectively , all of which being larger than that of peiminine (6.86 mg·kg-1). Corresponding results were obtained in the calculation of the LD50 95% confidence intervals of the 6 drugs. ConclusionAll the five derivatives of peiminine were shown to have lower toxicity than that of peiminine and it is hopeful to find antitussives of high potency and low toxicity from these derivatives.
  • 药物研究
    Effect of Glycyrrhetic Acid on the L-type Calcium Current of Ventricular Myocardial Cells of the Rat
    XIE Shirong;SONG Zhiguo;YU Xinyu;SUN Changbin;HUANG Caiyun;HUANG Shengying;LIU lin
    2005, 24(8): 0-0.
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    ObjectiveTo survey the effect of glycyrrhetic acid (Gta) on the L-type calcium current of single ventricular myocardial cells of the rat. MethodsAdult Wistar rats of both sexes served as the subjects of the experiment. The isolated rat heart was submitted to constantspeed perfusion with a Langendorff device. Single ventricular myocytes were isolated from the rats heart by emzymolysis with the use of type Ⅰcollagenase. A small amount of the suspension of myocytes was instilled into a bath with a volume of 0.2－0.3 mL. 0.1, 1.0 and 10.0 mo1·L-1 of Gta were then successively added into the bath with equal time intervals. Wholecell patchclamp technique was used to record the effect of Gta in different concentrations on the L-type Ca2＋ current (Ica-L) of single rat ventricular myocytes. The Ca2＋ current was elicited under those conditions in which the retention potential was 30 mv, the instruction potential was －40－±60 mv, the steps pulse was 10 mv, the duration was 300 ms, and the stimulus frequency,0.5 Hz .The currentvoltage (I-V) curve of IcaL in different concentrations of Gta was plotted with the instruction potential and the results of the action of the corresponding Ca2＋ channel current. ResultsGta in concentration of 0.1,1.0 and 10.0 μmol·L-l was shown to reduce IcaL in a dosedependent manner: the Ica-L value before the addition of Gta (2.30±0.29)nA was lowered to (1.96±0.34), (1.37±0.24) and (0.66±0.20) nA, respectively (P＜0.01). Gta in concentrations of 0.1,1.0 and 10.0 μmol·L-l could also inhibit the I-V curve of Ica-L, causing it to move upward, while the ICa-max remained unchanged. ConclusionGta was shown to inhibit the inflow of Ltype Ca2＋ by blocking the L-type Ca2＋ channel. This action of Gta may be related to its antiarrhythmic effect.
  • 药物研究
    Determination of the Content of Dexamethasone Sodium Phosphate in the Scala Tympani Perilymph and Blood Plasma in Guinea Pigs after Intracardiac Injection of the Drug
    ZHANG Shunguo;CHEN Minling;LI Fang;YANG Jun;HOU Dongming;WU Hao
    2005, 24(8): 0-0.
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    ObjectiveTo set up a method for the determination of the concentration of dexamethasone sodium phosphate in perilymph and blood plasma of guinea pigs. MethodsHPLC was used for the determination of the content of dexamethasone sodium phosphate in the samples of perilymph and blood plasma after intracardiac injection of the drug .HPLC conditions: A ZORBAX Eclipse XDB-C18 column (4.6 mm×250 mm,5 μm)served as the stationary phase,the mobile phase was 7.56 mmol·L-1 ammonium sulfate acetonitrile (70∶30) , with a flow rate of 1.0 mL·min-1; the column temperature was 20℃, the wavelength for detection, 239 nm. ResultsThe calibration curve of dexamethasone sodium phosphate was linear within the concentration range of 0.05－4.80 mg·L-1(r＝0.999 8), the lower limit of detection was 6.25 μg·L-1, the intraday and interdays RSD were 1.7% and 2.3%. One hour after the administration of the drug, the concentrations of dexamethasone sodium phosphate were 0.08 mg·L-1 and 2.77 mg·L-1 in the perilymph and blood plasma ,respectively. ConclusionThe method was shown to be sensitive, accurate and well reproducible ,it can thus be used for the determination of the concentration of dexamethasone sodium phosphate in perilymph and blood plasma. Our results also suggest that systemic medication of the drug is not a suitable method for the treatment of diseases of the inner ear. Other routes for the administration of the drug, therefore, should be sought.
  • 药物研究
    Effect of Verapamil on the Serum Tumor Necrosis Factorα in Rats with Peritoneal Adhesion Formation
    WANG Yong;CHEN Daoda;TIAN Yuan;ZHANG Jinghui
    2005, 24(8): 0-0.
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    ObjectiveTo study the changes in serum levels of tumor necrosis factor α (TNF-α) during the process of peritoneal adhesion in rats and the effect of verapamil on the formation of adhesion. Methods40 adult male SpragueDawley (SD) rats were randomly divided into 4 equal groups:1. sham operation group, 2. model group, 3. 0.9% sodium chloride solution group and 4. verapamil group .A median abdominal wall incision was made in each rat under pentobarbital sodium anesthesia. In rats of the sham operation group, the cecum was placed to the corresponding position of the right lateral perietal peritoneum before closure of the incision. The model of peritoneal adhesion was set up in the other 3 groups of rats by creating local injuries on the surfaces of the cecum and the right abdominal wall followed by suturing of the two roughened surfaces. Before closure of the incision, rats of group 3 and group 4 were given each 1 mL of 0.9% sodium chloride solution and 1 mL of verapamil solution containing 2 g of the drug per liter, respectively. The solutions were instilled onto the wound surface and into the abdominal cavity. Serum contents of TNF-α were determined before the operation and 1 day after the operation. The severity of the abdominal adhesion was assessed 7 days after the operation.ResultsThe postoperative serum TNFα levels in rats of group 2(model) and group 3(treated with 0.9% sodium chloride solution) were significantly higher than those in rats of group 1(sham operation)(P＜0.01). In contrast ,no striking increase in serum TNF-α content was shown in rats of group 4 treated with verapamil(P＞0.05). No peritoneal adhesion was formed in rats of group 1(sham operation). Severe peritoneal adhesion was formed in rats of group 2 (model) and group 3 treated with 0.9% sodium chloride solution. In the 10 rats of group 4 treated with verapamil, 6 animals showed no adhesion at all and the other 4 had only mild adhesion (P＜0.01). ConclusionIt is thought by the authors that TNF-α may exert a promotive effect on the formation of peritoneal adhesion. It is also possible that verapamil may reduce the severity of peritoneal adhesion by preventing a rise in serum TNF-α levels.
  • 药物研究
    Drug Sensitivity of Acute Refractory Leukemia Cells to Chemotherapeutic Agents
    TIAN Huaiping;BU Shuhong;LI Fang;YANG Jingming
    2005, 24(8): 0-0.
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    ObjectiveTo study the drug sensitivity of acute refractory leukemia cells to 13 chemotherapeutic agents in vitro.Methods37 bone marrow specimens from patients with acute refractory leukemia were collected from which leukemia cells were separated for the preparation of leukemic cell suspensions .The drug sensitivity of the leukemic cells to 13 chemotherapeutic agents was assayed with the MTT methods and the rates of inhibition of these agents on the different leukemic cells were determined. ResultsAcute nonlymphocytic leukemia (ANLL) cells and acute lymphocytic leukemia (ALL) cells were sensitive to etoposida (VP16) and cylosine arabinoside(Ara-C) but not to daunorubicin(DNP),epirubicin(EPI),pirarubicin(THP),cisplatin(DDP) and mytomycin(MMC).The rate of inhibition of teniposide(VM-26) and etoposide(VP-16) on ALL cells was higher that on ANLL cells .In contrast ,the rate of inhibition of mitoxantrone(MIT) on ANLL cells was higher than that on ALL cells.ConclusionIn general, acute refractory leukemia should be submitted to a chemotherapeutic plan containing VP-16 and Ara-c. ALL could better be treated with a chemotherapeutic plan containing VM-26 and VR16 while ANLL should be treated with a chemotherapeutic plan containing MIT.
  • 药物研究
    Fructus Lycii Glycopeetide Enhances the Effect of Radiotherapy in Tumorbearing Mice
    DU Guang;FANG Jianguo;WEI Shichao
    2005, 24(8): 0-0.
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    ObjectiveTo probe into the enhancement of the effect of radiotherapy in tumorbearing mice by Fructus Lyeii glycopeptide(FLG) capsules. Methods40 mice of the Kunming strain were randomly divided into 4 equal groups:1.model group,2.radiotherapy group,3.radiotherapy ＋highdose FLG group and 4. radiotherapy ＋ lowdose FLG group. Mice of group 3 and group 4 were given each 8 mg·kg-1 and 4 mg·kg-1 of FLG solution , respectively .The drug was administered by gastrogavage ,q.d.. Mice of group 1 ang 2 were given each equivalent amounts of 0.9%sodium chloride solution by the same route .The course of treatment in all 4 groups lasted 4 days.On the 4th day of the experiment and after the last gastrogavage, animals of all 4 group were given each a hypodermic inocubation of 0.2 mL of Ehrlich's ascites cancer cell suspension containing 1×106 cells·mL-1. Mice of group 2,3 and 4 were subjected to 60Co radiotherapy on the 7th day and 10th day separately, with a dose of 8 Gy. radiation for each mouse. Mice of all 4 groups were sacrificed by cervical dislocation on the 15th day .The weight of the tumor, level of lymphocyte transformation expressed by stimulation index(SI) and activity of NK cells in each animal of the 4 groups were examined and registered.ResultsThe weight of the tumor was decreased in all animals subjected to radiotherapy. However, the decrease of the tumor weight in animals pretreated with FLG in high dose and low dose was more remarkable than that in mice that had not been pretreated with FLG. There was a dramatic fall of SI in mice of group 2 submitted only to radiotherapy. However, the fall of SI was much less remarkable in mice of group 3 and group 4 pretreated with FLG(P＜0.01).The decrease of the NK cell activity in mice of group 3 and group 4 pretreated with FLG was also less significant than that in mice of group 2 subjected only to radiotherapy(P＜0.05). ConclusionFLG was shown to enhance the anticancer effect of radiotherapy remarkably. It could also notably mitigate the immunodepression caused by radiotherapy. FLG is therefore a good enhancer of radiotherapy.
  • 药物研究
    The Effect of Bushenzhuangyang Decoction on Climacteric Rats with Insufficiency of KidneyYang
    SI Huiliang;CHEN Youxiang;HOU Anji;XIE Dan
    2005, 24(8): 0-0.
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    ObjectiveTo study the therapeutic effect of bushenzhuangyang(BSZY) decoction on the model of female climacteric rats with insufficiency of kidneyyang. Methods30 female SD rats at their natural climacteric age of 11－15 months were randomly divided into 3 equal groups:1.model(M) group,2. M＋BSZY treatment group and 3.Climacterium group.The model of insufficiency of kideneyyang was set up by giving an I.M. injection of 2 mg·(100 mg) -1 of cortisol to each rat at 8 a.m. q.d. for 14 consecutive days. In addition, 10 female SD rats at the age of 3－5 months served as young controls. Beginning from day 15,that is ,1 day after the model had been successfully set up ,rats of group 2 were given each by gastrogavage 10 mL·kg-1 of BSZY decoction at 9 a.m.q.d.for 14 consecutive days .Rats of group 1,2 and young controls were given each equivalent amounts of 0.9%sodium chloride solution by the same route for 14 days as well. On day 29, blood samples were collected from rats of all 4 groups for the determination of serum levels of estradiol (E2) , follicle-stimulating hormone(FSH) and luteinizing hormone(LH). ResultsIn comparison with rats of the model group (group 1) , rats of group 2 treated with BSZY had a depilation of milder degree , better appetite,amelioration of polyuria and chilly sensation. as well as a striking increase in body weight(P＜0.01).The serum level of E2 in rats treated with BSZY was significantly higher than that of the model group(P＜0.01),while the levels of serum FSH and LH in rats treated with BSZY were apparently lower than those in rats of the model group (P＜0.01 or P＜0.05).The serum level of E2 in rats of the model group was much lower than that of rats in the climacterium (group 3) (P＜0.01).Conclusion BSZY was shown to be effective in the treatment of insufficiency of kidneyyang in climacteric rats.KEY WORDSBushenzhuangyang decoction; Climacteric syndrome; Insufficiency of kidneyyang; Rats(Note: 1. The bushenzhuangyang decoction is composed of 13 traditional Chinese medicines including Radix achyranthis bidentatae, Radix morindae officinalis and Fructus lycii, etc. 2. The kidneyyang is defined by the traditional Chinese medicine as the energy or motivating force for the physiological function of the kidney as well as the source of life process. 3. The insufficiency of kidneyyang if described as a morbid condition due to constitutional anomaly, chronic illness, excessive consumption of the kidneyyang, or senile debility, manifested as aversion to cold, cold limbs, listlessness, weakness and soreness of the loins and knees, impotence, spermatorrhea, nocturia, pale and corpulent tongue with thick whitish coating and weak chi pulse.)
  • 药物研究
    A Study of the Antiinflammatory Components of Smilax China
    RUAN Jinlan;ZOU Jian;CAI Yaling
    2005, 24(8): 0-0.
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    ObjectiveTo study the active antiinflammatory components of Smilax China and their antiinflammatory effects in rats. MethodsThe chemical components of the ethyl acetate portion of Similax China were separated and purified repeatedly with silica gel column chromatography. The structures of the chemical compounds Ⅰ and Ⅱ thus obtained were then authenticated with infrared analysis, mass spectrometry and nuclear magnetic resonance (including 1H-NMR,13C-NMR and 2DNMR),separately. The antiinflammatory activity of the compounds Ⅰ and Ⅱ was assayed by observing the effect of the compounds on the cotton ballinduced granuloma in rats after these compounds had been administered by gastrogavage.ResultsThe compoundsⅠandⅡ isolated were identified as reverstroal(Ⅰ) and oxyreverstroal(Ⅱ). Reverstroal (Ⅰ) in a dose of 0.2 mg·kg-1·d-1 was shown to inhibit the cotton ballinduced granuloma in rats(P＜0.05). ConclusionReverstroal (Ⅰ) was found to be one of the antiinflammatory components of Smilax China.
    • 药物与临床
  • 药物与临床
    Treatment of 80 Cases of Schizophrenia Manifesting Acute Erethitic Symptoms with Quetiapine Combined with Clonazepam
    WANG Dongbo;XU Songquan;TANG Qingping;YING Yifei;MEI Yingtai;HE Faxiang
    2005, 24(8): 0-0.
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    ObjectiveTo study the therapeutic effectiveness and safety rate of quetiapine combined with clonazepam in the treatment of schizophrenics manifesting acute erethitic symptoms. Methods160 schizophrenics suffering from acute erethitic symptoms were randomly divided into two equals groups. 80 cases of the treatment group were given each 100－500 mg of quetiapine PO q.d.. and 2－4 mg of clonazepam I.M.q.d.. 80 patients of the control group were given each an I.M. injection of 10－20 mg of haloperidol ,q.d.. The course of treatment in both groups lasted 7 days.During the course of treatment, the Positive and Negative Syndrome ScaleExcited Component (PANSS-EC) and the Treatment Emergent Symptoms Scales (TESS ) were assessed every day.ResultsThe effective rates of quetiapine and haloperidolin in controlling acute erethitic symptoms in the 2 groups were 78.0% and 80.0%,respectively,the difference being insignificant(P＞0.05) . The incidence of extrapyramidal adverse reactions in patients of the control group was higher than that of the treatment group. Common adverse reactions in patients of the treatment group were somnolence, dryness of mouth, blurred vision, nausea and vomiting, dizziness, tachycardia, hypotension etc.. ConclusionQuetiapine combined with clonazepam was shown to exert definite therapeutic effect in controlling acute erethitic symptoms of schizophrenics and its safety rate was higher than that of haloperidol.
  • 药物与临床
    A Comparative Study of the Effect of MydrinP and Atropin Eye Drops on the Result of Optometry in Children
    WU Jianguo;CHEN Yong
    2005, 24(8): 0-0.
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    ObjectiveTo survey the accordance rates of mydriasis optometry and callback examination after the application of mydrinP and atropin eye drops in children with ametropia.Methods186 children with ametropia (372 eyes) served as the subjects of the study. The ametropic eyes of the patients were subjected to quick mydiasis by receiving mydrinP eye drops every 5 min. for three times. Optometry was carried out 30 min after the last dropping . Callback examination was performed on the next day after the pupils had reverted to the normal size. Beginning from the same day, the patients were given atropine eye drops t.i.d. for 3 consecutive days for their ametropic eyes. Optometry for these patients was once more performed on the 4th day and callback examination carried out 3 weeks later . ResultsHyperopia accounted for the majority of ametropia(66.88%) in children below 10 years of age while myopia was more prevalent(64.15%) in children 10－14 years old. In children 10－14 years old who suffered from myopia ,the difference between the results of optometry after mydriasis induced by mydrinP and atropin, as well as the difference between results of callback examination after mydrinP and atropine treatment ,was insignificant(P＞0.05). These differences, however, were significant (P＜0.01 or P＜0.05) in patients with hyperopia who were 10－14 years old as well as in children under 10 years of age who had myopia or hyperopia.ConclusionMydrinP was shown to be an ideal drug for mydriatic optometry in children 10 －14 years old who suffered from myopia. However, in children younger than 10 years old suffering from myopia or hyperopia , as well as in children shown to have compound astigmatism as revealed by optometry following mydrinP, atropin is indicated.
  • 药物与临床
    The Effect of Penehyclidine Hydrochloride as a Premedicant before Anesthesia
    LUO Huiyu;WANG Peng;TIAN Yuke
    2005, 24(8): 0-0.
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    ObjectiveTo survey the effects of preanesthesic application of penehyclidine hydrochloride on the blood pressure and heart rate of patients during anesthesia as well as postoperative secretions from the respiratory tract and adverse reactions. Methods60 patients of ASAⅠ－Ⅱ grade scheduled for upper abdominal operation were randomly divided into three equals groups: group Ⅰ,Ⅱand Ⅲ.All these patients had no diseases of the cardiovascular and nervous systems. The patients were asked to rest for 15 min .after their entrance into the operating room .Patients of group Ⅰ,Ⅱand Ⅲ were then given each an intramuscular injection of0.5 mg of atropine, 0.3 mg of scopolamine and 0.5 mg of penehyclidine hydrochloride ,respectively. 20 min thereafter anesthesia was induced by intravenous injections of midazolam, fentanyl and atracurium . All patients were subjected to endotracheal intubation and mechanical ventilation during the operation. Systolic blood pressure(SBP), diastolic blood pressure (DBP), heart rate(HR),and degree of blood oxygen saturation(SpO2) were registered before medication(T0), 10 min.(T1) and 20 min.(T2) after medication ,before induction of anesthesia (T3),before enotracheal incubation (T4),immediately after intubation (T5),10 min. after intubation (T6),at the termination of operation(T7) and during decannulation (T8) .The degree of tranquilization and anxiety was assessed during the process of premedication, and the amount of secretions from the respiratory tract was measured when the operation was terminated. Adverse reactions, if any, were kept under close observation. Results Blood pressure levels of the patients of group Ⅲ pretreated with penehycline hydrochloride were shown to lie closer to the normal range. The systolic blood pressure of these patients at T2,T3 and T4 was lower than that of the patients of group Ⅰand groupⅡpretreated with atropine and scopolamine,respectively, the differences being significant(P＜0.05).Similarly, the diastolic blood pressure of the patients pretreated with penehycline hydroclaride was lower than that of patients pretreated with atropine and scopolamine ,respectively ,at T2 ,T3 ,T4 and T5,the differences being significant too(P＜0.05).The amount of secretions from the respiratory tract in patients pretreated with penehyclidine hydrochloride was smaller than that in patients pretreated with atropine, the difference being significant(P＜0.05).ConclusionAs a premedicant before anesthesia,penehyclidine hydrochloride was shown to exert milder effects on heart rate and blood pressure.It had also a stronger inhibitory effect on glandular secretion in the respiratory tract than atropine and scopolamine.
    • 药品质量控制
  • 药品质量控制
    Quantitative Assay of Ethylenediamine Diaceturate in Its Injection with Nearinfrared Diffuse Reflectance Spectroscopy
    JIANG Yan;MA Changqing
    2005, 24(8): 0-0.
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    ObjectiveTo set up an method for the quantitative determination of ethylenediamine diaceturate in its injection with nearinfrared diffuse reflectance spectroscopy. MethodsAll spectra were surveyed with a Fourier transform spectrometer equipped with a InGaAs detector and a fibreoptic probe for reflectance measurements. All samples were scanned from 12 000·cm-1 to 4 000·cm-1，and each sample spectrum was obtained as an automatic mean of 64 scans. Spectral region7 482.5－5 041.1·cm-1 was selected to develop mathematical models by the partial least square method.ResultsThe root mean square error of prediction (RMSEP) obtained from the validation of 10 unknown samples was 2.03，the correlation coefficient between the true value and prediction value was 0.996 9. The average rate of recovery was (100.85±0.29)%，and RSD of the method precision, 0.28%. ConclusionThe results show that the method is accurate, reliable, simple and convenient for the determination of ethylenediamine diaceturate in its solution. No pretreatment is required. It is suitable for analyzing high batch samples.
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