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    药物研究
  • 药物研究
    Effect of Recombinant Human Acidic Fibroblast Growth Factor on Promoting Rehabilitation of Burned Skin in Animals
    LI Yiwu;LI Guiling;LI Yue;XIANG Jizhou;LIU Yongmei;FU Xiaobing
    2005, 24(11): 0-0.
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    ObjectiveTo study the effect of recombinant human acidic fibroblast growth factor (rhaFGF) on promoting rehabilitation of the operated wound skin of piglets and of the burned skin of rabbits. Methods(1) The wound models on skin of piglets were made and divided into groups, among which three groups received 90，60 and 30 AU·(cm2)-1 rhaFGF, the positive group received 60 AU·(cm2)-1rhbFGF, the negative group received only the base of the formulation and the blank control group received nothing. The wound area was recorded by photos and cellophane film at 4, 8, 14 d. The volume of injured cavity was measured with water injection. At 8, 14 d, the growth of wound granulation and re-epithelization were tested to evaluate the effect of rhaFGF. (2) The burned skin models of rabbits were made, divided into groups and treated in the same way as with the piglets. Observe the wound area after 3, 7, 14, 21 d. After 14, 21 d, examine the growth of wound granulation and re-epithelization to evluate the treatment. ResultsThe wound skin of the piglets became smaller as time went on in all groups, and in 90 AU·(cm2)-1 and 60 AU·(cm2)-1rhaFGF groups significantly smaller than in the other groups. The volume of injured cavity grew smaller, and in 90 AU·(cm2)-1 and 60 AU·(cm2)-1 rhaFGF groups more significantly. After 8 d, fibroblasts and capillary granulation tissue grew more and actively, in 90 AU·(cm2)-1 and 60 AU·(cm2)-1rhaFGF groups significantly more than in the control group. After 14 d, all the wounds were closed up. (2) All the wounds of the rabbits became smaller as time went on, and in the 90 AU·(cm2)-1 and 60 AU·(cm2)-1 rhaFGF groups significantly more than in the others. After 14 d，new epithelization covered most wound area and nearly recovered in rhaFGF 90 AU·(cm2)-1.ConclusionRhaFGF can promote rehabilitation of the wound skin of piglets by promoting the growth of granulation tissue, of capillary angiogenesis and of epithelium. RhaFGF can promote the recovery of burned skin of rabbits by promoting the growth of granulatuion tissue and epithelization.
  • 药物研究
    Determination of Monosaccharides in Rheum Tanguticum Maxim Polysaccharides by High Performance Capillary Electrophoresis
    ZHAO Yan;LIU Li;YANG Xingbin;LI Xiaoye;SUN Xiaoli;ZHANG Shengyong
    2005, 24(11): 0-0.
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    ObjectiveTo determine the monosaccharides in rheum tanguticum maxim polysaccharides and their molar ratio. MethodThe rheum tanguticum maxim polysaccharides were hydrolyzed into monosaccharides with 2 mol·L-1 sulfuric acid. The monosaccharide derives obtained with 1-phenyl-3-methyl-5-pyrazolone (PMP) were determined by capillary zone electrophoresis (CZE) with fused silica capillary tube (70 cm×50 μm, effective length is 60 cm), diodearray detector, 150 mmol·L-1 sodium tetraborate buffer (pH value was 10.8), 10 kV, 25 ℃, and 0.5 psi pressure injection for 5 s. ResultsEight monosaccharides derivatives were isolated with high sensitivity, good resolution by CZE. The rheum tanguticum maxim polysaccharides are composed of xylose, arabonose, glucose, rhamnose, galactose, mannose, glucuronic acid and galacturonic acid with a molar ratio of 1.00∶23.27∶15.93∶2.95∶1.34∶49.07∶2.62∶4.31. ConclusionThis method is simple, rapid, sensitive and can be used to determine the monosaccharides in rheum tanguticum maxim polysaccharides.
  • 药物研究
    Bioeguivalent Study of Two Fluconazole Capsules in Human Plasma
    QIU Xiangjun;LI Yan;XU Peng;HU Guoxin;DAI Zongshun
    2005, 24(11): 0-0.
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    ObjectiveTo study the relative bioavailability and bioequivalence of two fluconazole capsules in human plasma. Method20 healthy volunteers in randomised crossover study were given a single oral dose of 300 mg of test or of reference. The plasma level of fluconazole was determined by HPLC. The pharmacokinetic parameters were calculated and the bioavailability and bioequivalence of two formulations were evaluated by 3p97 program. ResultsThe pharmacokinetics parameters for test formulation and reference were Cmax,(6.271±0.874) and (6.374±0.806) mg·L-1;tmax, (1.825±0.591) and(1.875±0.998) h; AUC(0-120),(239.408±47.330) and (243.061±29.497) mg·h·L-1; AUC(0-inf),(255.835±51.738) mg·h·L-1 and (261.675±35.995) mg·h·L-1, respectively. The 90% confidential interval of Cmax, AUC(0-120) and AUC(0-inf) of the test were 92.8%－104.0%, 90.3%－104.9% and 89.5%－104.3%, respectively. The relative bioavailability was (99.0±18.7)%. ConclusionThe two formulations are bioequivalent.
  • 药物研究
    Bioeguivalent Study of Two Glipizide Capsules in Human Plasma
    XIA Chunhua;LI Lixin;ZHANG Xinjing;LI Yanyan
    2005, 24(11): 0-0.
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    ObjectiveTo establish a HPLC method for the determination of two glipizides and their bioequivalence in male volunteers. Method1.0 mL serum sample was added to C18 cartridges, and then washed with 1.0 mL 20% methanol solution. The cartridge was eluted with methanol. The residue from the eluate evaporated to dryness at 60 ℃ was dissolved in methanol and injected into a Diamonsil ODS column (4.6 mm×150 mm, 5 μm). The mobile phase was purified water (pH value＝3.65, adjusted with acetic acid)acetonitrile (51∶49), the flow rate 1.0 mL·min-1, and the detect wavelength 275 nm. A single oral dose of 10 mg of test capsule or reference capsule was given to 18 volunteers in randomized in two groups with crossover design. ResultsThe linear range was within 20－960 ng·mL-1, r＝ 0.999 9. The limit of detection was 20 ng·mL-1. The recovery rate was 98.2%－105.7%. Their withinday precision RSD and betweenday precision RSD were less than 10%. The main pharmacokinetic parameters had no significant difference between two preparations. ConclusionThis method is sensitive, specific and suitable for the determination of glipizide concentration in human plasma. The two preparations are bioequivalent.
  • 药物研究
    Immunity Modulating of Arbidol Hydrochloride in Mice
    ZHOU Yiping;JIANG Jingyi;CHEN Siyan;CHEN Qiyou;SUN Xuebing;YANG Jinghua
    2005, 24(11): 0-0.
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    ObjectiveTo observe the effects of arbidol hydrochloride (Ar) on interferon inducing and immunity modulating in mice.MethodThe mice were intragastrically given Ar of 100, 50 or 25 mg·kg-1. Then the serum interferon were timely determined after a single Ar administration. The rate of abdominal phagocytosis of macrophage in normal mice, the index of phagocytosis, the carbon particles clearance index and delayed type hypersensitivity in immunesuppressed mice induced by Hydrocortisone (Hc), and the contents of serum hemolysin both in normal and immunesuppressed mice induced by Cyclophosphamide (Cy) were detected.ResultsThe interferon contents were detected in 6～24 h after Ar administration, and the maximum peak was in 18 h. An increased phagocytosis of peritoneal macrophage in normal mice, the enhanced carbon particles clearance index and delayed type hypersensitivity in immunesuppressed mice induced by Hc and the higher contents of serum hemolysin both in normal and immunesuppressed mice induced by Cy were observed with the administration of Ar for 5 d. ConclusionAr exerts enhancing effects on interferon induction in vivo and on immunity function both in specifically and nonspecifically.
  • 药物研究
    Protective Effect of Isoliensinine on Oxidative Injuried Erythrocytes
    WANG Hui;YANG Shuqing;LI Hua
    2005, 24(11): 0-0.
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    ObjectiveTo study the protective effect of Isoliensinine (IL) on the oxidative injuried erythrocytes. MethodUsing the methods of autooxidation , H2O2 and ·OH induced oxidation to erythrocytes, observe the effect of IL on hemolysis degree and on the serum level of malondialdehyde (MDA). ResultsErythrocyte hemolysis induced by autooxidation and activated oxidation with H2O2 and ·OH was inhibited by IL. and the content of MDA was lowered. ConclusionIsoliensinine has protective effect on the oxidative injuriederythrocytes.
  • 药物研究
    In Vitro Release Experiment of Huperzine A Sustainedrelease Microspheres and Its Correlation Evaluation between In Vivo and In Vitro
    FU Xudong;GAO Yongliang;PING Qineng;TANG Ren
    2005, 24(11): 0-0.
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    ObjectiveTo establish a method for the determination of in vitro release of huperzine A sustainedrelease microspheres with a good correlation between in vivo and in vitro. MethodImmediate release and dialysis release methods were used to determine the in vitro release rate of huperzine A microspheres. Effects of tween concentration and the medium volume in dialysis bag on release rate were studied. The residue huperzine A in the injection site was determined by HPLC, and in vivo release rate of huperzine A microspheres was caculated. The optimal release solution was chosen based on the correlation evaluation between the in vivo and in vitro results.ResultsDialysis release method had a good in vitro and in vivo correlation (r＝0.990 2).ConclusionDialysis release method could be used to determine the in vitro release of huperzine A from microspheres.
  • 药物研究
    Protective Effect of Danshen Injection on Myocardial Ischemia in Rabbits with Acute Renal Failure
    LIU Jiubo;CHEN Lin;ZHU Shaoming;PENG Xinmin;LAN Yongshe;Li Yan
    2005, 24(11): 0-0.
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    ObjectiveTo study protective effect of danshen injection on myocardial ischemia in rabbits with acute renal failure (ARF). Method 28 rabbits were randomly divided into three groups: the control group,n＝8; the model group n＝12; and danshen group,n＝8. The control group and the model group were injected sodium chloride injection, and danshen group injected danshen injection, iv, 0.5 mL·kg-1, four times daily for 4 d. Then the control group were injected 10 mL·kg-1 0.9% sodium chloride injection, and the model group and danshen group were injected 10 mL·kg-1 50% glycerin by pressure injection in the behind legs 0.5 h later since the first injection. The platelet aggregation, PGI2, TXA2, endothelins (ET) and myocardial morphology were examined. ResultsCompared to the control group, at 2 and 24 h, platelet aggregation, TXA2, ET were significantly higher, and 6-Keto-PGF1a were significantly lower (P＜0.01), and there were severe ischemic injury in the model group; but not significantly in danshen group. At 2 and 24 h, in danshen group, platelet aggregation, TXA2, ET was significantly lower, and 6-Keto-PGF1a was significantly higher than in the model group (P＜0.01). Myodardial ischemic injury in danshen group was markedly reduced.ConclusionThe higher platelet aggregation, and the more vasoactive substance and oxygen free radical during ARF may be one of the direct causes of ischemic myocardial injury. danshen injection may reduce the cardiac ischemic injury in rabbits with acute renal failure by inhibiting blood platelet conglomeration and lowering TXA2, and ET.
    • 五官科用药专栏
  • 五官科用药专栏
    Pharmacodynamic Study of Yanlaotong Granules
    CAI Huafang;JIANG Youfang;ZUO Junbin;SHEN Huagang
    2005, 24(11): 0-0.
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    ObjectiveTo study the effects of yanlaotoang granules on anticoagulation, antiinflammation and phagocytosis. MethodThe experimental animals were divided into 3 treatment groups, danshen group and the control group. The treatment groups received 5，10，20 g·kg-1yanlaotoang granules, ig; danshen group received danshen injection of 5 g·kg-1, ip; the control group received pure water, ig, for 10 d. Coagulation time, bleeding time，ear swelling induced by croton oil，capillary permeability of abdominal cavity induced by acetic acid and phagocytosis of peritoneal macrophages in mice were tested. ResultsIn the treatment group, the coagulation time and bleeding time were significantly prolonged; the ear swelling induced by croton oil relieved; the abdominal capillary permeability suppressed and the phagocytosis of peritoneal macrophages promoted. ConclusionYanlaotong granules have anticoagulation and antiinflammation effects and promote phagocytosis. It may improve eye circulation by reducing inflammatory exudation.
  • 五官科用药专栏
    Inhibition of Fluvastatin on Hyperplasia of In Vitro Human Eye Tenon’s Capsule Fibroblasts
    LI Jing;XIONG Xinchun;TONG Xiaoyun
    2005, 24(11): 0-0.
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    ObjectiveTo explore the antiproliferation effect of fluvastatin on cultured Tenon's capsule fibroblasts in vitro. MethodThe Tenon's capsule fibroblasts isolated from glaucoma patients were cultured and subcultured in vitro. The proliferation of fibroblasts were detected by MTT method after incubation with 1×10.9, 1×10.8, 1×10.7, 1×10.6, 5×10.6, 1×10.5, 2×10.5 mol·L-1 fluvastatin for 24, 48, and 72 h. ResultsA490 values by MTT assay were lower as the concentration of fluvastatin increased or the incubation time extended. After 24 h, A490 values were significantly lowered in the concentration of fluvastatin above or equal to 5.0×10.6mol·L-1than the control (P＜0.01). After for 48 h, A490 values were significantly lowered in the concentration of fluvastatin above or equal to 1.0×10.6 mol·L-1than the control (P＜0.05). After for 72 h, A490 values were significantly lowered in the concentration of fluvastatin above or equal to 1.0×10.7mol·L-1than the control (P＜0.05).ConclusionFluvastatin can inhibit the proliferation of human Tenon's capsule fibroblast cells dose and timedependently in vitro. The results suggest that fluvastatin may be effective in the treatment of ocular fibroproliferative disorders.
  • 五官科用药专栏
    BCG-PSN on the Treatment of 30 Patients with Spring Conjunctivitis
    HU Jun
    2005, 24(11): 0-0.
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    ObjectiveTo observe the clinical effect of BCG-PSN on conjunctivitis in spring and on serum levels of T cell subsets and of cytokines. Method60 patients with conjunctivitis were equally randomized into two groups. The treatment group were given BCG-PSN 1(＞13 a)or 0.5 mg(≤13 a), im, three times a week for 8 weeks besides the conventional treatment. The control group was treated conventionally. The levels of total IgE, of T cell subsets and cytokines were determined before and after treatment. IL-4 and INF-γ were determined by ELISA. ResultsThe levels of total IgE in treatment group were significantly lower than that of the control group (P＜0.01) after the treatment. And the levels of CD＋8 were lower, meanwhile, the levels of CD＋4 and of CD＋4/CD＋8 were much higher, significantly (P＜0.05). And the level of IL-4 were significantly lower, and levels of INFγhigher (P＜0.05). ConclusionBCG-PSN can regulate immunity function, coordinate the homeostasis of CD＋4 and CD＋8 cells, and reduce the occurrence of anaphylaxis. BCG-PSN may inhibit the Th2 and reduce the synthesis of IL-4, and strongthenTh1. BCGPSN can help the recovery of conjunctivitis in spring.
  • 五官科用药专栏
    Therapeument of Chitooligosaccharide in 30 Patients with Gingivitis in Fixed Orthodontics
    LONG Xiaosi;LING Hui;SHEN Zhenxiang
    2005, 24(11): 0-0.
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    ObjectiveTo study the effects of chitooligosaccharide on gingivitis in fixed orthodontics. Method60 fixed orthodontic patients were randomly divided into treatment group and control group. Chitooligosaccharide was orally given to the treatment group, nothing to the control group. Gingival index (GI), plague index (PLI) and simple calculas index (CI-S) were measured and compared before and after treatment. ResultsAfter treatment for 2, 4 weeks, GI in the treatment group were significantly decreased 28.8%, 32.9%; and PLI 32.3%, 34.7%, than in the control group (P＜0.01). But the CI-S had no change. ConclusionChitooligosaccharide is effective in the treatment of gingivitis in fixed orthodontics.
    • 药物与临床
  • 药物与临床
    Therapeument of Ibandronate in 28 Patients with Bone Metastases from Pulmonary Cancer
    SHI Xing;WANG Jing
    2005, 24(11): 0-0.
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    ObjectiveTo evcluate the analgesic effect of ibandronate and its adverse drug reaction (ADR) in patients with bone metastases from pulmonary cancer. Method28 patients diagnosed bone metastases from pulmonary cancer were given 4 mg ibandronate in 500－750 mL sodium chloride injection or 5% glucose injection, iv, for more than 2 h infusion, 1 time every 4 wk for 8 wk. ResultsThe analgesic rate of ibandronate was 75.0%. And the rate of ADR was relatively low, for example, fever. ConclusionIbandronate which is kind of bone absorption inhibitor is effective and safe for patients with metastatic bone tumor in analgesia treatment.
    • 药物制剂
  • 药物制剂
    Preparation of Buanfen Capsules and Its Quality Control
    HUANG Liangyong;TU Ziliang;DU Shiming;YE Fang
    2005, 24(11): 0-0.
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    ObjectiveTo prepare buanfen capsules and to establish a quality control method．MethodThe content of buanfen in capsules was determined by HPLC．The column was C18 (200 mm×4.6 mm, 5 μm)，the mobile phase was 0.01 mol·L-1 phosphate solutionacetontrile (43∶57, V/V), and the detection wavelength was 220 nm. ResultsThe calibration curve of ibuprofen had good linearity within the range of 1.75－35 μg·mL-1 (r＝0．999 7) and paracetamol had good linearity within the range of 2.0－400 μg·mL-1. The average recoveries of ibuprofen and paracetamol were 99.8% and 100.5%(RSD＜1.5%).ConclusionThe preparation of buanfen capsules is simple, the contents of ibuprofen and paracetamol are determined by HPLC with one operation, and its quality control is reliable．
    • 药品质量控制
  • 药品质量控制
    Determination of Adenosine in Folium Isatidis by RPHPLC
    WANG Wenqing;ZHANG Fei;FANG Jianguo;MA Yonggui;TANG Jie;LI Yuanyuan
    2005, 24(11): 0-0.
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    ObjectiveTo establish a method for the assay of adenosine in folium isatidis .MethodThe adenosine in folium isatidis were determined by RPHPLC. The stationary phase: Lichrosorb C18 (4.6 mm×150 mm, 5 μm), the mobile phase: methanolwateracetic acid (1∶96∶3), the flow rate: 0.8 mL·min-1 and the detective wavelength: 257 nm. ResultsThe linear range of adenosine was within 1.52－48.64 μg·ml-1(r＝0.999 2).The average recovery rate was 99.75%(RSD＝1.91%), 98.64%(RSD＝0.41%), 98.80%(RSD＝1.24%) (n＝3), respectively. ConclusionThe method is sensitive, accurate and reliable.
  • 药品质量控制
    Determination of Cholesta4,6-dine-3-ol in Turtleback by TLCScanning
    LEI Juntao;CAI Bailing
    2005, 24(11): 0-0.
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    TObjectiveTo establish a TLCscanning method for the assay of cholesta4,6-dine-3-ol in turtleback. MethodThe samples were scanned by dualwavelength reflection method with the wavelength of detection λS＝450 nm, and the reference wavelength λR＝640 nm .ResultsThe calibration curve for cholesta4,6-dine-3-ol had good linearity within the range of 0.2－4.5 μg ·g-1 (r＝0.999 94). The average recovery rate and RSD were 96.5% and 2.6% (n＝6), respectively. ConclusionThis method can remove the interference of other components in turtleback and is convenient, reliable and repeatable.
    • 用药指南
  • 用药指南
    Antimicrobial Resistance Analysis of Pseudomonas Aeruginosa and Staphylococcus Aureus
    HAO Rui;JIANG Caie;CHEN Weibing;CHENG Xiaonan
    2005, 24(11): 0-0.
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    ObjectiveTo provide evidence of pathogen for clinical therapy, the distribution and antimicrobial resistance of pseudomonas aeruginosa and staphylococcus aureus in recent years were analyzed. MethodP. aeruginosa strains and S. aureus strains were cultured and identified by conventional techniques. Tests of antibiotic sensitivity were performed with KirbyBauer (K-B) method. ResultsThe antibiotic resistance rate of P. aeruginosa strains to ciprofloxacin, amicaxin, imipenem, piperacillin, ceftriaxone, cefoperazone and gentamycini were 16.9%, 20.0%, 22.8%, 30.9%, 33.6%, 36.4% and 64.8%. The antibiotic resistance rate of S. aureus strain to vancomycin, ciprofloxacin, amoxicillin/clavulanic acid, oxacillin, erythromycin and penicillin was 11.0%, 25.4%, 29.8%，47.9%，71.8% and 78.9%.ConclusionThe antimicrobial resistances of P. aeruginosa strains and S. aureus strains which were the main pathogen bacteria inducing hospital infection, must be paid more attention.
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