Archive

• 01 February 2004 Volume 23 Issue 2

    

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  药物研究
  老年病用药专栏
  药物与临床
  药品质量控制
  

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药物研究
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  药物研究

  The Prophylacticotherapeutic Effect of Leizhusun Juice on the Liver Injury Induced by Carbon Tetrachloride in Rats

  LIU Tongyun;SHU Sijie;SHU Hui

  2004, 23(2): 73-0074.

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  To probe into the prophylacticotherapeutic effect of leizhusun juice (LZS) on the experimental liver injury in rats. Methods:44 wistar rats were randomly divided into four groups. 8 animals of group 1 served as blank controls. Rats of group 2,3 and 4(with 12 animals in each group) were given each by gastrogavage normal saline, 205.70 mg·kg1 of LZS and 436.40 mg·kg1 of LZS in equal volumes respectively, q.d. for 7 consecutive days. After the last medication, each animal of group 2,3 and 4 was given an intraperitoneal injection of 0.1 mL/100 g body weight of 50% CC14 in peanut oil. 72 hours later blood was taken from each of the 8 surviving animals of the four groups and serum separated for the determination of the activities of alanine aminotranferase (ALT), aspartate aminotranferase (AST) and superoxide dismutase (SOD) as well as the content of malonyldialdelyde (MDA). Results:CC14 was shown to cause a sharp rise in the serum levels of ALT, AST and MDA and a striking fall of the serum SOD level in animals of group 2. In rodents of group 3 and group 4 that had been pretreated with two different doses of LZS before CC14 poisoning the serum levels of ALT, AST and SOD were significantly lower while the serum content of MDA was significantly higher than those of the animals of group 2 (P＜0.05,P＜0.01). A doseeffect relationship of LZS was also demonstrated. Conclusion:LZS was shown to have a distinct prophylscticotherapeutic effect directed against acute liver injury induce by CC14 in rats, the underlying mechanism maybe related to its effect of antioxidation.
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  药物研究

  Influence of Coumarins from Radix Angelicae Dahuricae on the Hypnotic Effects of Pentobarbital Sodium and Barbital Sodium in Mice

  WANG Decai;GAO Yunsheng;LI Tongde;MA Jian;ZHAO Xiaomin;LI Juan

  2004, 23(2): 75-0076.

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  To survey the influence of the coumarins from radix angclicae dahuricae(CAD) on the hypnotic effects of pentobarbital sodium and barbital sodium in mice. Methods:60 Runming mice were randomly divided into 5 equal groups, with 12 animals in each group. Mice of group 1, 2, 3, 4,and 5 were given each by gastrogavage 20 mL·kg1 of 5% tween80, 25 mg·kg1 of chloromycetin, 25, 50 and 100 mg·kg1 of CAD in equal volumes, respectively. One hour later, all of the animals were given each an intraperitoneal injection of 40 mg·kg1 of pentobarbital sodium or 200 mg·kg1 of barbital sodium. The latent period and duration of hypnosis of the mice were kept under observation. Results:The duration of hypnosis induced by pentobarbital sodium was prolonged by 280.9%, 185.1%, 252.2% and 266.9%, respectively in mice given chloromycetin and 3 different doses of CAD (P＜0.01). 100 mg·kg1 of CAD was shown to significantly prolong the latent period (P＜0.01) and shorten the duration of hypnosis (P＜0.01) induced by barbital sodium.Conclusion:CAD is considered by the authors of the present study to act by inhibiting the liver microsomal cytochrome P450. CAD in high dosis seems to excite the central nervous system in mice.

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  药物研究

  The Relation between the Antineoplastic Effect of Group A Streptococcus and Cytokines

  ZENG Gang;XU Peijun;ZOU Zhihong

  2004, 23(2): 77-0078.

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  To probe into the mechanisms of the antineoplastic effect of group A streptococcus preparation sapeilin at the cytokine level. Method:Sapeilin in concentrations of 0.5, 0.1, 0.02 and 0.004 KE·mL1 was cocultured with peripheral blood lymphocytes (PBL) form tumor patients. After 24, 48 and 72 hours of coculture, respectively the PBL were subjected to assay of their cytotoxicity directed against K562 cells and Raji cells with the MTT method. Meanwhile detection of IL12, IL2, IFNγ and TNFα in supernatants of the PBL was carried out either with FLISA or measurement of bioactivity. Results:The cytotoxic effects of and the cytouines produced by the PBL which had been cocultured with sapeilin in different concentrations and for different periods of time were shown to be different. Sapeilin in concentrations of 0.1 and 0.02 KE·mL1 was shown to enhance the cytotoxic effect of PBL directed against tumor cells and high levels of IL12, IL2, IFNγ and TNFα could also be detected in the supernants. Sapeilin in a concentration of 0.5 KE·mL1 was shown not only to depress the cytotoxic effect of PBL directed against tumor cells but also decrease the amounts of cytokines produced by the PBL. Conclusion:The cytotoxic effect of PBL from tumor patients directed against tumor cells was shown to be related to the concentration of sapeilin coincubated with the PBL. The tumoricidal effect was also shown to be related to the production of cytokines.
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  药物研究

  A Comparative Study of the Depressant Effects of Human and Bovine Lactoferrins on the Replication of Hepatitis C Virus in Vitro

  HONG Jun;QIN Chengming;LIN Yulin

  2004, 23(2): 80-0082.

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  To probe into the depressant effects of human lactoferrin(HLF) and bovine lactoferrin(BLF) on the replication of hepatitis C virus (HCV) in HepG2 cell culture, so as to provide theoretical as well as experimental basis for the clinical management of HCV infection.Methods:HepG2 cell culture was used as the host of HCV tranfection and replication HCV positive chain RNA and negative chain RNA were assayed with RTnested PCR. The cytotoxic effect of the lactoferrins on HepG2 cells was examined with the MTT test. Results:Both human and bovine lactoferrins in high concentrations were shown to exert cytotoxic effects on HepG2 cells However when the concentration of the lactoferrins was 1.5 mg·mL1 or lower, no cytotoxicity was present. Both HCV positive chain RNA and negative chain RNA could be detected from the cell extract within several days after the HepG2 cells had been inoculated with HCV RNA positive serum. If HLF or BLF (at concentrations of 1.5 mg·mL1, 1.0 mg·mL1 and 0.5 mg·mL1) had been coincubated with HCV positive serum for a period of time before addition of HepG2 cells or coincubated with HepG2 cells followed by the admixture of HCV positive serum, no HCV positive chain RNA nor HCV negative chain RNA could be detected in the cell extract with RTnested PCR. If, however the LF (at the same concentrations as mentioned above) coincubated with the HepG2 cells had been washed away before the addition of HCV positive serum both HCV positive chain RNA and negative chain RNA were demonstrated in the cell extract with RTnested PCR. Taken togethers the findings of these 3 experiments suggest that the depressant effects of the LF in noncytotoxic concentrations on the replication of HCV in HepG2 cells inoculated with HCV positive serum may be attributed to binding of the LF to corresponding sites on the virus. It seems unlikly that these effects were caused by interaction between the LF and the HepG2 cells. The difference between the effects of HLF and BLF was insignificant. Conclusion:HLF and BLF in noncytotoxic concentrations were shown to exert a depressant effect on the replication of HCV in HepG2 cell culture inoculated with HCV positive serum, possibly by binding of the LF to corresponding sites on the virus.
老年病用药专栏
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  老年病用药专栏

  The Therapeutic Effectiveness and Influence on Serum TNFα and sICAM1 of Shuxuetong in the Treatment of Patients with Acute Cerebral Infarction

  WANG Song

  2004, 23(2): 84-0086.

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  To probe into the therapeutic effectiveness and influence on serum tumor necrosis factorα(TNFα) and soluble intercellular adhesion moleculeI(sICAM1) of shuxuetong in the treatment of patients with acute cerebral infarction. Methods:115 patients with acute cerebral infarction were randomly divided into 2 groups, the treatment group(n＝60) and control group(n＝55). Patients of the treatment group were given each 4 mL of shuxuetong injection in 250 mL of 0.9% sodium chloride solution administered by IV instillation q.d.. Patients of the control group were given each 0.4 g of troxerutin in 250 mL of 0.9% sodium chloride solution administered by IV instillation as well q.d.. Conventional treatment for patients of both groups was the same. The course of treatment in the two groups lasted 14 days. The nerve function scorings were noted down and contents of serum TNFα and sICAM1 determined before the treatment and on day 3 and day 14 of the treatment. The barthel index on the 90th day after the beginning of the treatment was recorded. Results:The therapeutic effectiveness in patients of the treatment group was superior to that of the control group, the difference being significant(P＜0.05). The decline in serum level of TNFα on day 3 of the treatment was more striking in patients of the treatment group than in the control group(P＜0.05). The decrease in the serum content of sICAM1 determined on day 3 and day 14 was significantly greater in patients of the treatment group than in the control group (P＜0.01). 3 months after the beginning of the treatment the Barthel index in patients of the treatment group(84.17±12.35) was evidently better that of the control group (70.43±13.28)(P＜0.01).Conclusion:Shuxuetong was shown to be effective and safe in the treatment of acute cerebral infarction probably by mitigating the noxious effects exerted on the brain cells by serum TNFα and sICAM1 that may be used as indices for the assesment of the patients condition and effect of treatment. The remote therapeutic effect of the drug was also shown to be good. No toxic or hypersensitive reactions were encountered.
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  老年病用药专栏

  Puerarin in the Treatment of 42 Cases of Acute Cerebral Infarction

  CAO Xuebing;LIU Changqin;YUAN Guanglei

  2004, 23(2): 87-0088.

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  To survey the effect of puerarin on hemorrheology and fibrin monomer polymerization in patients with acute cerebral infarction. Methods:84 cases of acute cerebral infarction were randomly divided into 2 equal groups, the puerarin group and the control group. Patients of the control group were treated with conventional measures including dehydration, brain protection, amelioration of microcirculation ect.. On the basis of these measures, patients of the puerarin group were given each 250 mL (600 mg) of puerarin administered by intravenous instillation q.d. for 10 consecution days. Hemorrheology and fibrin monomer polymerization were assayed before as well as on day 10 of the treatment. Results:The overall effectives rates in the puerarin group and control group were 95.23% and 76.19%, respectively(P＜0.05). Hemorrheology and the function of fibrin monomer polymerization in patients of the puerarin group were significantly ameliorated as compared with those of the control group(P＜0.01).Conclusion:Puerarin was shown to be effective in the treatment of acute cerebral infarction possibly by improving hemorrheology and the function of fibrin monomer polymerization.
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  老年病用药专栏

  A Clinical Study of the Treatment of Parkinson's Disease with αDihydroergocryptine in Combination with Compound Levodopa

  TONG Xiaoxin;TONG Etang

  2004, 23(2): 89-0090.

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  To assess the therapeutic effect and safeness of αdihydrorgoecryptine(αDHEC) in the treatment of patients with Parkinson's disease in the intermediate or advanced stage that had been treated with compound dopa (Ldopa/benseragide or Ldopa/carbidopa sustained release tablets) with failing drug effects. Methods:36 patients with Parkinson's disease in the intermediate or advanced stage that had been treated with compound dopa with failing drug effects were given each additionally 4 ml of αDHEC PO b.i.d, for 8 consecutive weeks. Webster's mark contrasting of the 10 major symptoms of the disease and examination of conventional laboratory indices were carried out before as well as 4 and 8 weeks after the beginning of the additional medication of αDHEC. Results:It was shown by Webster's marking that moderate effect and slight effect could be confirmed in 10 (27.8%) and 15 (41.7%) patients respectively. The differences between the Webster's markings of each of the 10 major symptoms of the disease before and after the additional medication with αDHEC were significant (P＜0.05). 4 of the 6 patients with "onoff" phenomenon became dramatically ameliorated in their clinical manifestation after the additional medication. The adverse reactions were mainly gastrointestinal. No other serious toxic and side effects were encountered. Conclusion:It proved safe and effective to add αDHEC to the treatment of patients with Parkinson's disease that had been treated with compound dopa with failing drug effects.
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  老年病用药专栏

  Treatment of 43 Cases of Vascular Dementia with Ginkgo Flavone Glycoside

  LI Dexiang;ZHANG Dongmei;LIU Juxiang;LI Dedong

  2004, 23(2): 91-0092.

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  To look into the therapeutic effectiveness and safeness of ginkgo flavone glycoside in the treatment of vascular dementia. Methods:86 patients with vascular dementia were randomly divided into two equal groups. Patients of the treatment group were given each 70 mg of ginkgo flavone glycoside in 300 mL of 0.9% sodium chloride solution administered by IV instillation q.d. for 15 consecutive days as a course of treatment, followed by 80 mg of ginkgo flavone glycoside PO t.i.d.. for 75 consecutive days, Patients of the control group were given each 0.8 g of piracatam injection in 300 mL of 0.9% sodium chloride solution administered by IV instillation q.d. for 15 consecutive days as a course of treatment followed by 0.8 g of piracetam PO t.i.d. for 75 days. 3 months later, patients of both groups were subjected to a second course of IV medication in the same doses of the drugs and the same duration of time as in the first course. The time of treatment in both groups lasted 6 months. The state of noopsyche and social functions in patients of both groups was tested with the MMSE and FAQ capacity tables respectively, before as well as 3 and 6 months after the beginning of the treatment. Results:The score of MMSE in patients of the treatment group 3 and 6 month after the beginning of the treatment was evidently superior to that in patients of the control group (P＜0.01). 6 months after the beginning of the treatment, the score of FAQ was strikingly superior to that in patients of the control group (P＜0.01). Conclusion:Ginkgo flavone glycoside was shown to cause a sustained improvement of the noopsyche and social functions and a slowdown of the progress of the disease in patients with vascular dementia. The drug was also found to be safe and no obvious side effects were encountered.
药物与临床
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  药物与临床

  Clinical Effect on Chronic Hepatitis B Treated by Famciclovir and Combination Famciclovir with INFα

  HUANG Jiaquan;TIAN Deying;HUANG Yuancheng;CHEN Yan

  2004, 23(2): 99-0099.

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  To observe the therapeutic effect of only famciclovir and combination famciclovir with INFα on chronic hepatitis.Methods:60 points were divided into two groups, one group of the 4th month of course treatment were treated by only famciclovir 500 mg three a day, the other group of combination famciclovir with INFα 3×106 U every two days for 6th month.Results:The result showed the famciclovir eliminating rates of HBVDNA and remission of serum ALT were close to that of combination famciclovir with INFα. The negative rate of HBVDNA was 66.7%, 70.0% respectively. The differentiation between the two groups had no obviously significance(P＞0.05). No obvious effect was founded.Conclusion:The result indicate the therapeutic method of only famciclovir may regulate liver of function, inhibit viral DNA and improve the therapeutic effect. Combination famciclovir with INFα can improve the HBeAg seroconversion rate.
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  药物与临床

  The Effects of Methylcobalamin Administered with Different Schemes in the Treatment of Diabetic Neuropathy

  FANG Zizheng;JIN Huiling;SHEN GengXin;CHENG Jinluo

  2004, 23(2): 101-0101.

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  To probe into the therapeutic effectiveness methylcobalamine administered by different schemes he treatment of diabetic neuropathy. Methods:66 patients with diabetic neuropathy were randomly divided into two group: group A(n＝31) and group B(n＝35). Patients of group A were given each 500 μg of methylcobalamin administered by intramuscular injection qd for 4 consecufive weeks. Patrents of group B were given each 500 μg of methylcobalamin administered by intramuscular injectim as well q.o.d. for 4 consecutive weeks. Results:Symptom amelioration increase in the conduction velocity of the common peroneal nerve and median nerve were noticed in patients of both groups. The difference between the therapeutic effects in patients of the two group was insignificant(P＞0.05). Conclusion:Methylcobalamin given intramuscularly every other day was shown to be reliable convenient as well as economic in the treatment of diabetic neuropathy.
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  药物与临床

  A Comparative Study of the in Vitro Antibacterial Activity of Two Drugs for Prevention of Neonatal Ophthalmia

  WANG Binghe;GAO Weisheng

  2004, 23(2): 102-0103.

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  To assay and compare the in vitro antibacterial activities of silver nitrate eye drops and povidoneiodine eye drops, both of which are used for prevention of neonatal ophthalmia. Method:Double broth dilution method was used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the two drugs. Results:For common pathogens of neonatal ophthalmia including staphylococcus aureus pneumocoecus and neisseria gonorrhoeae, the MIC50 of silver nitrate eye drops was 4, 8 μg·mL1 while the MBC90 was 8, 32 μg·mL1. The MIC50 and MBC90 of povidoneiodine eye drops for the pathogens tested were 64, 128 μg·mL1 and 256 μg·mL1, respectively. Conclusion:Both silver nitrate eye drops and povidoneiodine eye drops were shown to be provided with bacteriostatic and bactericidal activities directed against common pathogens of neonatal ophthalmia in vitro and the former proved to be more applicable to the prevention of the disease.
药品质量控制
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  药品质量控制

  Determination of the Content of Emodin in Poshangfeng Mixture with RPHPLC

  WANG Hu;ZHENG Jing;CHEN Huating;ZONG Haiyan

  2004, 23(2): 118-0119.

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  To determine the content of emodin in poshangfeng mixture with reversedphase HPLC for quality control of the preparation. Method:Emodin was separated with a hypersil C18 column. A mixture of methanol 0.1% phosphoric acid in water (85∶15) was used as the mobile phase the UV detection wavelength being 290 nm.Results:The linear range was 0.019 5－0.175 5 μg. The average rate of recovery of emodin was 97.3%, RSD＝3.29%(n＝5). Conclusion:The method was shown to be quick simple and convenient with high accuracy and reproducibility. It is therefore applicable to quality control of poshangfeng mixture.
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  药品质量控制

  Determination of the Content of Ferulic Acid in Xinshenghua Chongji with RPHPLC

  GUO Limin;LI Xianwei;TANG Liying;REN Zhiqiang

  2004, 23(2): 120-0120.

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  To set up a method for determining the content of ferulic acid in xinshanghua chongji. Method:RPHPLC was adopted in the study. An ODSC18 column was used for separation. A mixture of water (containing 1.0% of glacial acetic acid)∶ acetonitrile∶ methanol＝70∶0.6∶30 served as the mobile phase with a flow rate of 1.0 mL·min1. The detection wave length was 320nm.Results:The linear range was 5.4－86.4 μg·mL1, r＝0.998 7. The average rate of recovery was 99.7%, RSD＝0.49%. Conclusion:The method was shown to be highly effective, quick and sensitive and therefore suitable for quality control of the preparation.