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    特约稿
  • 特约稿
    Mechanisms of Adenosine Receptors Involved in Transmitting and Modulating Nociception*
    WU Le;XIE Shouzhen;LI Yunqing
    2004, 23(11): 795-0798.
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    Receptors belong to the family of G proteincoupled receptors, which includes four subgroupsA1，A2A，A2B, and A3 receptors. Adenosine receptors are widely existed in nervous systems. Activations of adenosine receptors in vivo produce a variety of effects such as regulating the release of neurotransmitters, the neuronal excitability, the control of motor function, and the modifying of emotional response. Adenosine A1 and A2A receptors are closely related to transmitting and modulating nociception, and their selective agonists and antagonists are promising in clinical practice. Therefore, the mechanisms of adenosine receptors involved in transmitting and modulating nociception are highlighted in the field of research work.
    • 药物研究
  • 药物研究
    Inhibitory Effect of Radix Isatidis on Activation of LPS-induced MAPK P38
    LIU Yunhai;HUANG Wei;TNAG Jie;HUANG Mingsheng;XIE Wei
    2004, 23(11): 799-0801.
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    To explore the effect of F022 part of radix isatidis on LPSinduced intracellular signal transmitting.Methods:After the respective treatments of BALB/C mice monocytes with six group samples (groups Ⅰ－Ⅳ were 1.000, 0.500, 0.250, 0.125 mg·mL1 F022 from radix isatidis with 100 μg·mL1 LPS, separately; groupⅤwas positive control100 μg·mL1 LPS; and group Ⅵ was negative control1 mg·mL1 F022). The P38MAPK activity in monocytes was detected. Results:By comparison to the control group, the inhibitory rates of 1.000, 0.500, 0.250, 0.125 mg·mL1 F022 on P38MAPK activity were 95.66%, 64.80%, 39.28% and 20.67%, respectively. Conclusion:F022 from radix isatidis inhibits activation of P38MAPK stimulated by LPS in doseresponse.
  • 药物研究
    Study of Water Extracting and Alcohol Precipitation in Ingredients Extraction Technique of Ganyanping Capsule
    DING Shuiping;FANG Jianguo;ZENG Hongxia
    2004, 23(11): 801-0805.
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    To explore the technique of water extracting and alcohol precipitation in the pharmaceutical technique of ganyanping capsules. Methods:The orthogonal design L9 (34) and L4(23) were respectively used in water extracting and in alcohol precipitation. Hydrosoluble extract was used as the assessment in the water extracting, and matrine rate was used in alcohol precipitation. The content of matrine was determined by HPLC, which used C18 (250 mm×4.6 mm, 5 μm) as column with phosphate buffer solution (pH value＝4.0, H2O 1 000 mL＋H3PO4 2.5 mL＋2.0 TEA)acetonitrile (90∶10) as the mobile phase. The detection wavelength was 210 nm. Results:The total extraction rate was markedly influenced by the factor D in water extracting. The best condition was D2A3B3C3. In alcohol precipitation, factor B played a more important role than factor A, and the A1B1 was chosen. Conclusion:Using matrine rate as the standard can objectively reflect the extraction technique, and the HPLC method for its determination is simple, rapid and accurate.
  • 药物研究
    Antagonistic Effects of Andrographolide on ADPinduced Platelet Aggregation in Vitro
    FANG Shuxian;ZHENG Heng;LIU Dong;WANG Hongwei;MA Baoxia
    2004, 23(11): 806-0807.
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    To investigate the antagonistic effects of alcoholic extract of andrographis paniculata (APN) and chuanhuning at different concentrations on ADPinduced platelet aggregation.Methods:After APN or APN monomer at different concentrations was added into the human platelet rich plasma (PRP), the ADPinduced platelet aggregation was measured by using multifunctional platelet aggregometer.Results:APN could inhibit the ADPinduced platelet aggregation in vitro in a dosedependent manner.Conclusion:Andrographolide can antagonize the ADPinduced platelet aggregation in vitro.
  • 药物研究
    Change of Serum sICAM1 in UAP Patients Treated by Danshen Injection
    HU Youzhuang;YANG Bin;LIU Dongbiao;FENG Dehui
    2004, 23(11): 809-0811.
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    To observe the change of soluble intercellular adhesion molecule1 (sICAM1), Creactive protein (CRP) between healthy man and the patients with unstable angina pectoris (UAP), and the effect of danshen injection on the level of sICAM1 and CRP in patients with UAP. Methods:Sixtyfour suitable patients with UAP were randomized into two groups, the first control group 31 cases, treated routinely and the treatment group33 cases treated by adding danshen injection to the former treatment. The serum levels of sICAM1 and CRP of the patients with UAP were tested before and after the treatment, and were compared to the second control group (22 healthy man) by ELISA. The clinical effects were observed.Results:The serum levels of sICAM1 and CRP in UAP patients were significantly higher than in the second control group (P＜0.01). After treatment, the level of sICAM1 and CRP in UAP patients were significantly lower than before (P＜0.01). The effect in treatment group was significantly superior than in the first control group (P＜0.05), however, ECG had no significant improvement (P＞0.05). Conclusion:The level of sICAM1 and CRP may be the risk factors of CHD and they may be used to predict prognosis. Danshen injection can lower the level of sICAM1 and CRP.
  • 药物研究
    Effects of Diammonium Glycyrrhizinate on Thrombus Formation and on Blood Coagulation in Animals
    LIU Cuixia;WU Jiliang;JIANG Nan;CAO Weili;GUO Wei
    2004, 23(11): 811-0813.
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    To study the effects of diammonium glycyrrhizinate on the formation of venous throumbus and on the blood coagulation in animals. Methods:Diammonium glycyrrhizinate were injected with a dose 10－20 mg·kg1 into the experimental animal (rabbits, rats and mice) veins. The effects of diammonium glycyrrhizinate on the thrombus formation and on the blood coagulation were observed 30 minutes later. Results:The doses of 10, 20 mg·kg1 diaminonium glicyrrhizinate inhibited the thrombus formation in rats, and the inhibiting rates in rats were 68.7%, 72.5%. And the inhibiting rates in rabbits were 12.7%, 26.7%. The diammonium glycyrrhizinate prolonged the time of blood coagulation in mice. By comparison to sodium chloride injection the results were significantly different(P＜0.01). Conclusion:Diammonium glycyrrhizinate can inhibit the thrombus formation and prolong the time of blood coagulation in the experiment animals.
  • 药物研究
    Antipulmonary Fibrosis Effect of Captopril in Rats
    YE Yanqing;XU Qiyong
    2004, 23(11): 815-0818.
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    To study the antipulmonary fibrosis effect of captopril (CPT) and its mechanism.Methods:Thirty SD rats were randomized into three groups: CPTtreated group, model group and control group evenly. The treatment group and the model group were intratracheally instilled with bleomycin (5 mg·kg1, 0.2－0.3 mL), and the control group received sodium chloride injection. Then the treatment group fed with CPT, and the control fed with sodium chloride injection until they were killed. Five rats in each group were separately killed 7 d later and 28 d later for experimental check. Lung weights, ACE activity were tested. The degrees of lung alveolitis and pulmonary fibrosis were checked by hemotoxylin and eosin colouration, and by HPIAS image analysis. Transforming growth factor (TGFβ1) was assessed by immunohistochemical technique. Results:The lung alveolitis and pulmonary fibrosis in the treatment group were significantly relieved by comparison to the model group. ACE activities in the treatment group were lower than in model group significantly 7 d later(P＜0.01), and in model group, ACE activities still higher 28 d later. TGFβ1 level in CPTtreated group was lower than model group 7 later, but there were no significant difference among the three groups 28 d later. Conclusion:Captopril has the effect of antipulmonary fibrosis in experimental rats by reducing ACE activity and inhibit expression of TGFβ1.
  • 药物研究
    Preparation of Junxianling Cream and Its Skin Allergy and Irritation Reaction Study
    DU Guang;ZHENG Heng;ZONG Kai
    2004, 23(11): 816-0817.
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    To prepare the junxianling cream and to observe its safety in external use. Methods:The prescription of junxianling cream and its preparation skill were established. Then its irritation reaction on normal and damage skin of rabbits and its allergy reaction on guinea pigs were carried out. Results:Junxianling cream had a white, fine and smooth and easytodaub quality. There were no swollen, red speckles or local skin irritation reaction observed on rabbits. There were no allergy reaction found on guinea pigs during 6－72 h after use.The prescription of junxianling is rational and the preparation has a good quality.
    • 代谢性疾病用药专栏
  • 代谢性疾病用药专栏
    Hypoglycemic Drug Analysis in Hospitals of Wuhan During 2001－2003
    CHEN Huating;YANG Rong;YANG Yingda
    2004, 23(11): 818-0820.
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    To analyze the usage of hypoglycemic drugs in hospitals of Wuhan. Methods:To investigate hypoglycemic drug consumption in Wuhan during the past three years in respect of money and DDDs. Results:The consumption and frequency of hypoglycemic drugs during 2001－2003 showed an upward attitude. The dose of insulin has been increasing steadily. Conclusion:Sulfonylures were the first in the sum of money, and diguanides were used most frequently.
  • 代谢性疾病用药专栏
    Effect of Glybenclamide on Apolipoprotein E Secretion from Macrophages
    ZHOU Xiaoqin;DONG Yin;YUAN Yonghui
    2004, 23(11): 821-0823.
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    To investegate possible effect of glybenclamide on apo E secretion from human THP1 macrophages, and to evaluate glybenclamide in treatment of type 2 diabetes. Methods:THP1 monocytes were cultivated from which the differentiation of THP1 macrophages was induced. Macrophage foam cells were prepared by loading macrophages with cholesterol. Both types of cells were incubated with glybenclamide. After incubation for 8 h, intracellular and secretary apo E from the cells were determined by ELISA. ApoE mRNA was determined by Northern blot.Results:After incubation with glybenclamide, apo E synthesis and apo E mRNA expression remained unaffected, apo E secretion from THP1 macrophages and from macrophage foam cells decreased significantly (P＜0.01). The decrease of secreted apo E in macrophages was more than in the macrophage foam cells. Conclusion:Glybenclamide impairs secretion of apo E from macrophages so that it may be unfavorable to lipid metabolism in patients with type2 diabetes, especially those with dyslipoproteinemia.
    • 药物制剂
  • 药物制剂
    Preparation of Multieffect Gastroscope Emulsion and Its Clinic Application
    YU Xiquan;YANG Zhongdong;FAN Shangtan;XIAO Hua
    2004, 23(11): 849-0850.
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    To study the prescription, preparation, quality standard and clinic effect of multieffect gastroscope emulsion. Methods:The emulsion was prepared by rubbing emulsification. Its stability was studied and the main drugs content was determined by UVspectrophotometry. The emulsion was administrated 10 mL, po, 30 min before gastroscopy. Its clinical effects were observed on 506 patients who needed gastroscopy. Results:By oral administration one time, good effects of local anaesthesia, relieved convulsion, reduced foam, decreased excretion, and lubrication were simultaneously achieved. The total effective rate was 100.0%.Conclusion:The prescription of multieffect gastroscope emulsion is rational, and its preparation is convenient. The emulsion has a stable quality and certain clinical effect, and its use should be recommended.
  • 药物制剂
    Determination of Active Amylopsin in Multienzyme Microtablet Capsule
    SU Yuyong;ZHANG Xueping
    2004, 23(11): 851-0852.
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    To establish a method for determination of the active of amylopsin in multienzyme microtablet capsule. Methods:The glucose hydrolyzed from starch by amylopsin catalysis was determined by iodimetry. One unit amylopsin is defined as 1 μmol glucose produced by hydrolysis in one minute. Results:The average recovery for amylopsin was 100.7% (RSD＝0.78%, n＝9). Conclusion:This method is simple, accurate, repeatable and high selective for the determination of the active amylopsin in multienzyme microtablet capsule.
  • 药物制剂
    Dissolution Study of Compound Antipyretic Gelatin
    BAI Xuemin;ZHANG Xueping;SUN Jianshu
    2004, 23(11): 853-0854.
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    To establish a dissolution standard for compound antipyretic gelatin.Methods:The dissolved effective ingredient was determined with 0.062 mol·L1 hydrochloric acid as the solvent according to the method in Chinese Pharmacopoeia. Results:The dissolved effective ingredient of compound antipyretic gelatin in 30 min was 89.8%. The test of postdifference was C＝0.009 8, P＝1. Conclusion:The Dissolution of compound antipyretic gelatin in 30 min should be 85.0%.
    • 药品质量控制
  • 药品质量控制
    Dtermination of Glycyrrhizic Acid in Weiling Granules by HPLC
    LI Chengwang
    2004, 23(11): 858-0859.
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    To establish a HPLC method for determination of glycyrrhizic acid in weiling granules.Method:The content of glycyrrhizic acid was determined by HPLC. The Hypersil C18 was the column; and methanol0.2 mol·L1 ammonium acetateacetic acid (66∶33∶1) was mobile phase. The detective wavelength was 254 nm.Results:The linearity response within the range 7.444－37.320 μg·mL1 was good (r＝0.999 5). The average recovery was 98.58%，RSD＝0.65%.Conclusion:The results show that the method is simple and accurate. It can be used to determine the glycyrrhizic acid in the product.
  • 药品质量控制
    Determination of Paeoniflorin in Liuweijianwei Granules by HPLC
    WANG Zhichao;LIU Zuxiong;LIU Hong;TANG Ren
    2004, 23(11): 859-0860.
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    To determine paeoniflorin in liuweijianwei granules by HPLC.Method:The stationary phase was Hypersil ODS column (4.0 mm×125 mm，5 μm), the mobile phase methanolwater (25∶75), and the detection wavelength 230 nm. Results:This method had a good linearity within the range 0.512－8.192 μg·mL1, r＝0.999 9. The average recovery was 100.45%，RSD＝1.85%(n＝5). Conclusion:This method is simple, quick, and accurate.
  • 药品质量控制
    Determination of the Concentration of Mannitol in Rabbit Plasma by Colorimetry
    LIU Xinyun;TU Ziliang;WANG Qibin;ZHAO Jifei;LI Shouhua;MA Changqing
    2004, 23(11): 861-0862.
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    To establish a method for determination of mannitol in rabbit plasma.Methods:The plasma samples was deproteinized with acetate trichloride, mannitol contained in supernatant was oxidated with sodium periodate deproteinized and further reacted with Nash reagent to form the colour product which can be detected at a wavelength of 405 nm. Results:The linear range of calibration curve was 0.188－6.00 mg·mL1, r＝0.999 7(n＝6). Corresponding to the drug plasma concentration. The average rate of recovery was 98.30%. RSD was 1.89%(n＝3).Conclusion:The method is rapid, simple and can be used for determination of plasma concentration of mannitol and its clinical pharmacokinetic study.
    • 用药指南
  • 用药指南
    Analysis of Antibacterial Drugs in Treatment of Community Acquired Infection
    DU Xiaoyi;YE Yan;WANG Linrun
    2004, 23(11): 869-0870.
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    To investigate the usage of antibacterial drugs in 200 community acquired infection patients.Method:Total 200 cases of community acquired infection in ward of respirology from January to June in 2003 were analyzed.Results:About 55 antibacterial drugs were involved in the treatment. The three most frequently used antibacterial drugs were cefoperazone/sulbactam for injection, levofloxacin tablets, levofloxacin injection. The average variety of antibacterial drugs per patient was (3.6±2.0). In addition, the three antibacterial drugs ranked in primacy in DDDs were levofloxacin tablet, cefoperazone/sulbactam for injection, levofloxacin injection. The daily consumption of antibacterial drugs for injection was more than for oral administration. Conclusion:The whole medical quality will be improved by using antibacterial drugs rationally. And there will be less drugresistance bacteria.
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