中国科技论文统计源期刊 中文核心期刊  
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊  
日本科学技术振兴机构数据库(JST)
第七届湖北十大名刊提名奖  

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    药物研究
  • 药物研究
    XING Mao;ZHANG Enjuan;YE Xin
    2002, 21(06): 331-333.
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    Objective:To investigate the possible role of caspase 3 and bcl2 in arsenic trioxide(As2O3)induced apoptosis of SGC7901 cell line. Methods:Cell apoptosis was assessed through agarose gel electrophoresis and the double staining of annexin V and propidium iodide (PI), of which fluorescent intensities were measured on flow cytometry. SGC7901 was treated with As2O3 and DEVDCHO, a selective inhibitor of caspase 3. Change in expressions of caspase 3 and bcl2 was observed by means of flow cytometry. Results:As2O3 upregulated caspase 3 and downregulated bcl2 protein. DEVDCHO inhibited the apoptosis induced by As2O3. Conclusion:As2O3 may selectively activate caspase 3 by downregulating the expression of bcl2 protein, which causes SGC7901 cells apoptosis.
  • 药物研究
    SHEN Lijun;LAI Yi;YU Jingyu
    2002, 21(06): 333-334.
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    Objective:To determine the in vitro inhibitory effects of Qinyin injection on dysentery bacillus and escherichia coli. Method:By means of doubling dilution method in broth in vitro, the MICs and MBCs of Qinyin injection and its related individual components against dysentery bacillus and escherichia coli were measured. Results:Qinyin injection and its individual components exhibited inhibitory or bactericidal effects on dysentery bacillus and escherichia coli. Conclusion: Qinyin injection had stronger inhibitory effects on bacteria than its individual components.
  • 皮肤病性病用药专栏
  • 皮肤病性病用药专栏
    CAO Yuchun;GONG Li;XU Zuseng;WAN Mufen
    2002, 21(06): 337-339.
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    Objective:To investigate the positiveresult rates of DNA of pathogens in male STU patients without symptoms after treatment.Methods:We chose the male patients who had the first onset of symptoms of urethritis as subjects of the study, and divided them randomly into groups A and B. All the subjects in group A were treated with levofloxacin, while those in group B with levofloxacin and azithromycin for two weeks, respectively. After treatment, DNA of Ng, Uu and Ct were detected by means of FQPCR. Then those with positive DNA of Ng, Uu and Ct (including patients with and without symptoms) were selected, and treated for two weeks, followed by detection of the DNA of Ng Uu and Ct again. Identification of those asymptomatic but positive pathogen DNA was made. Results:The positive rates with DNA of Ng, Uu and Ct among the subjects without symptoms in groups A and B were 35.2% and 20.8%, respectively, after 2 weeks posttreatment, and 50.0% and 15.8%, respectively, after 2 weeks further of treatment was still postive DNA of pathogens.Conclusion:The positive rates DNA of Ng, Uu and Ct DNA among the subjects without symptoms were high in the male STU patients after treatment. Further treatment should be focused on asymptomatic patients with positive pathogen DNA.
  • 皮肤病性病用药专栏
    LI Shenqiu
    2002, 21(06): 340-341.
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    Objective:To observe the infection and drug resistance of mycoplasma in male venereal patients. Methods:To the samples of 5 354 patients who were suspected of venereal diseases, Neisseria gonorrhoeae culture, mycoplasma culture and drug susceptisility testing were done. Results:In these patients, the positive rate of pathogen was 24.9%(1 333/5 354). Among them, the proportion of neisseria gonorrhoeae, chlamydi trachomatis (Ct), ureaplasma urtalyticum (Uu), mycoplasma hominis (Mh) and Uu confirmed with Mh was 5.0%(67/1 333), 31.4% (418/1 333), 57.2%(763/1 333), 6.4%(85/1 333) and 9.1%(121/1 333) respectively. The result of drug susceptibility testing indicated that 54.7% was Ofloxacine resistance, 44.9% was erythromycine, 19.9% was tetracycline, 19.6% was doxycycline, 15.6% was josamycine and 11.6% was pristinamycine one. Conclusion:In male venereal patients manifestation of urethritis, Uu was the major pathogen. Most of venereal patients was between 21 and 40. Drug resistance of mycoplasma was severe. Culture and drug susceptibility testing of mycoplasma were important for clinical therapy.
  • 皮肤病性病用药专栏
    LIU Houjun;TU Yating;LIU Zhixing;LIN Nengxin
    2002, 21(06): 342-344.
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    Objective:To explore the role of Ureaplasma urealytica (Uu) and Mycoplasma hominis (Mh) in nongonococcal urogenital tract infection and examine their in vitro drug susceptibility. Methods:Detection of mycoplasma and susceptibility test of ureaplasma urealytica to 10 antimicrobial agents were performed by using microdilution method. Results:Among the 296 suspected cases, 101(34.1%) were positive with mycoplasma , 75 (28.8%) with Uu, 6(2.0%) with Mh, and 20 (6.8%) with Mh plus Uu. The positive rate with mycoplasma infection was significantly higher among the female subjects (42.9%) than that among the male (28.8%). The drug susceptibility test showed that, among the 10 antimicrobial agents tested, resistance of Uu was most commonly found to spiramycin (100.0%), and least found to josamycine (7.9%). Frequency of resistance of Uu, among the tested cases, to the other antimicrobial agents, was as followes: to ofloxacin (37.3%), to tetracycline (37.3%), minocycline (33.3%), doxycycline (32.0%), erythromycine (30.7%), levofloxacin (18.7%), azithromycin (13.3%), and to roxithromycin (10.7%). The crossresistance to fluoro quinolones , tetracycline and macrolides was found. The highest rate of crossresistance was between fluoro quinolores and tetracycline (12.0%). Conclusion:Monitoring the variance of resistance of mycoplasma is of important significance for guiding the use of drugs.
  • 皮肤病性病用药专栏
    CHEN Junhong
    2002, 21(06): 344-345.
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    Objective:To observe the efficacy of CO2 laser in the treatment of urethra condyloma acuminatum(CA). Methods:In this study, 100 cases of urethra condyloma acumination were recruited: 56 cases were treated with CO2 laser (group CO2 laser), while the other 44 cases with 20% peltatin(group peltatin). The relapse rates of the two groups were compared after three months of treatment. Results:The relapse rates of groups CO2 laser and posalfilin were 21.4% and 54.5%, respectively. The difference between the two groups were statistically significant (P<0.01). Conclusion:The CO2 laser therapy is superior to the peltatin therapy in respect of the prognosis of patients with urethra condyloma acuminatum.
  • 皮肤病性病用药专栏
    CHEN Xingping;LI Shenqiu;LI Jiawen;CEHN Yinling;XU Shizheng
    2002, 21(06): 346-347.
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    Objective:To evaluate the efficacy and safety of 1% clindamycin hydrochloride gel in the treatment of acne vulgaris. Methods:The effects of 1% clindamycin hydrochloride gel on acne vulgaris were observed in comparison with 1% clindamycing phosphate gel in a multicentre, doubleblind, randomized, and parallel controlled trial. Results:The effective rate in experimentgroup was 84.0% and the effective rate in controlgroup was 78.0%. No significant difference was found in efficacy between the two groups using ridit test. The side effects of both medicine were mild. Conclusion:1% clindamycin hydrochloride gel is safe and effective in the treatmeat of acne vulgaris.
  • 皮肤病性病用药专栏
    WU Yan;TAN Zhijian;LIU Zhixiang
    2002, 21(06): 348-349.
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    Objective:To compare the clinical efficacy of mizolastine (M) and cetirizine (C), terfenadine (T), loratadine (L) in the treatment of acute urticaria. Methods:Volunteers were divided into the following groups: group M (10 mg, po, qd, 37 subjects), group C (10 mg, po, qd, 35 subjects), group T (60 mg, po, bid, 34 subjects), group L (10 mg, po, qd, 34 subjects). The efficacy was evaluated at one hour, three and six hours, twentyfour hours after oral intake of the various drugs. Results:The effect was quicker and stronger in group M than that in groups C, T and L(P<0.05) after 1 hour. The effect was similar in groups M, C and T after 3 and 6 hours (P>0.05), but all stronger than group L (P<0.05). The effect was similar in groups M, T and L after 24 hours (P>0.05), but all stronger than group C (P<0.05). Conclusion:Misolastine is a quickacting and ultralente drug for acute urticaria. It is worthwhile to be used clinically.
  • 药物与临床
  • 药物与临床
    HU Shuhong;YUAN Gang;ZHANG Muxun
    2002, 21(06): 353-354.
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    Objective:To investigate the influence of IV infusion of xylitol on blood glucose and Cpeptide in diabetic patients. Methods:45 diabetic patients including 40 with type 2 and 5 with LADA were recruited for the study. All the subjects were administrated 5% xylitol 500 mL intravenously daily for 2 weeks; the infusion was completed in 2 hours, The plasma glucose and C peptide levels were determined before and after the first day of infusion. The blood transminase (ALT and AST) and renal function (BUN and Cr) were examined before and after the 2 weeks of treatment. Results:There was no significant difference between the plasma glucose levels before and after IV infusion of 500 mL of 5% xylitol (11.31±4.72 mmol•L1 versus 10.97±5.27 mmol•L1,P>0.05,ttest); the plasma C peptide level was significantly increased after Ⅳ infusion of 500 mL of 5% xylitol ( Median 2.01 μg•L1, Range 0.38~18.63 μg•L1 versus Median 5.00 μg•L1, Range 0.64~14.73 μg•L1,P<0.05, MannWhitney Utest). After 2 weeks, there were no significant changes in liver and renal function parameters, and no other side effects were found. Conclusion:The IV administration of xylitol can stimulate beta cells to produce insulin and maintain the blood glucose level without obvious side effects on the liver and kidney functions.
  • 药物与临床
    LI Jinrong;DIAO Dipeng;LIANG Huashan
    2002, 21(06): 355-356.
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    Objective:To explore an effective intervention for preventing the complications occurred in the hyperthyroid patients postthyroidectomy.Methods:A total of 372 cases of thyroidectomy were recruited and divided into groups 1 and 2. All the patients in group 1 were given iodine orally (15 drops, 3 times daily initially, and then decreased 1 drop daily progressively till 3 drops, 3 times daily, and then stop), while those in group 2 given dexamethasone intravenously (20 mg daily for 3 days) as a substitute for idione. Results:In group 1, 10 patients developed signs of hyperthyroidism crisis and the volume of bleeding ranged from 300 to 350 mL; 70 patients developed hyperplasia of scar. In group 2, 5 patients developed signs of hyperthyroidism crisis and 5 hyerplasia of scar. No bleeding was observed in this group. No death occurred in both groups. Conclusion: Administration of dexamethasone as a substitute for idione in hyperthyroid patients postthyroidectomy could prevent the occurrence of the main complications, such as hyperthyroidism crisis, bleeding and hyperplasia of scar, and decrease length of stay in hospital for 5-6 days.
  • 药物与临床
    GUO Junping;YAN Huaxiu;PENG Caiqin;FENG Yinjie;MA Lie;ZHANG Jinan;CHEN Qian
    2002, 21(06): 356-358.
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    Objective:To establish a method for the determination of rifampicin concentration in the body fluid of patients with tuberculosis (TB) by HPLC, and study correlation of the concentrations in different body fluids. Methods:The rifampicin concentrations in plasma and the pleural fluid and ascites were determined by HPLC method in 34 patients with TB.Results:The rifampicin concentrations in plasma and the pleural fluid as well as ascites were linearly correlated (r=0.999 3). RSD of intraand interday were less than 10%. The rifampicin concentration in plasma peaked in the 34 patients in 2 hours after administration of rifampicin. At 12 hours after administration of rifampicin, the concentrations in plasma, pleural fluid as well as ascites were still higher than the MIC (0.02 μg•mL1). Conclusion:The rifampicin concentrations in the pleural fluid and ascites were significantly correlated with that in the plasma. Administration of Rifampicin once daily could maintain its concentration at effective level at the target sites in the body.
  • 药品质量控制
  • 药品质量控制
    CAO Jian;CHE Keke
    2002, 21(06): 370-371.
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    Objective:To determine the contents of chloramphenicolum and dexamethasoni phosphas natricus in the chloromycetin compound eyedrops. Methods: The first order derivative spectrophotometry was used to determine the content of chloramphenicolum, and UV absorption was used to determine the total amount of chloramphenicolum and dexamethasoni phosphas natricus for the calculation of the content of dexamethasoni phosphas natricus. Results:The chloramphenicolum curve was linear in the content range of 25.06-75.18 μg•mL1, the first order derivative spectrogram was linear at 307 nm (C=1 393.510 5 D+0.037 2,r=0.999 9), the recovery is 100.5%,RSD=0.61%; The UV absorption spectrogram was linear at 242 nm (C=102.525 6 A-0.712 2,r=0.999 9),the recovery of dexamethasoni phosphas natricus is 99.0%,RSD=0.53%. Conclusion:The method used in this study was easy and practical
  • 药品质量控制
    LU Yongning;CHEN Dongsheng;XU Chuhong
    2002, 21(06): 372-373.
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    Objective:To establish a Jimanzhi RPHPLC method to determine the content of baicalin in Jimanzhix mixture. Methods:HYPERSIL C18 column was used. The mobile phase was consisted of methanolwaterphosphoric acid (48∶52∶0.2). The detecting wavelength was 280 nm. Results:The detecting ranges were 0.004 8-0.096 0 mg•mL1 .The average recovery of baicalin is 98.85%, RSD=2.07%(n=6).Conclusion:This HPLC method is convenient, accurate, stable and is suitable for measuring the content of baicalin in jimanzhi mixture.
  • 用药指南
  • 用药指南
    MA Ke
    2002, 21(06): 384-386.
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    Objective:To elucidate the situation and tendency of the use of drugs in the author’s hospital, and compare them with those in other hospitals in the Hangzhou District. Methods:The analysis was made with the data provided by the Development Center of Science and Technology of Chinese Pharmaceutical Association and that by the Information System of the author’s hospital. Results:The total expenses used for the purchase of drugs has been increasing year by year during the period of 1997~2000. However, the growth rate of the expense for drugs is descend; the ratio between the expense for antimicrobials and the total expenses for drug purchase is descend year by year. Conclusion: The use of drugs in our hospital is towards a rationalized way.