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  • 01 June 2015 Volume 34 Issue 6
      
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  • Effects of Periplocin from Cortex Periplocae on Apoptosis of Human Lung Cancer A549 Cells and Expression of Survivin
    ZHANG Jing, ZHANG Chao, YANG Guang, SHAN Baoen, LIU Jianghui
    2015, 34(6): 705-709. https://doi.org/10.3870/yydb.2015.06.001
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    Objective To investigate the effects of periplocin from Cortex Periplocae (CPP) on apoptosis of human lung cancer A549 cells and expression of survivin, and demonstrate its anti-tumor effect and the possible mechanism. Methods Inhibitory effect of CPP at different concentrations (1.25, 2.50, 5.00, 10.00, 20.00 ng·mL－1) and for different time length (24, 48, 72 h) on A549 cell proliferation was tested by MTT method.Apoptosis rate of A549 cells treated with CPP at different concentrations (2.50, 5.00, 10.00 ng·mL－1) were measured using flow cytometry (FCM) for 6, 12, 24, 48, 72 h, respectively.The morphological and ultrastructural changes of the apoptosis cells were observed by acridine orange/ethidium bromide (AO/EB) staining and transmission electron microscopy (TEM).The effects of CPP on mRNA and protein expression of apoptosis associated gene survivin were assessed by RT-PCR and Western blotting. Results CPP could significantly inhibit the growth of A549, and the inhibition rate reached (93.46±2.35)%.The results of FCM showed that the apoptosis rate of A549 cells treated with CPP was increased significantly as compared to the control group (P＜0.05).Meanwhile, typical apoptotic peaks were detected.The characteristic morphological changes of apoptosis were observed in A549 exposed to CPP, including cell shrinkage, the nuclei became yellow-red by AO/EB staining, and typical ultrastructural changes, including nuclear chromatin condensation along the nuclear membrane, vacuolar degeneration of cytoplasm observed by TEM.The result of RT-PCR indicated that survivin mRNA expression decreased obviously in A549 cells exposed to CPP.The protein expression of survivin in A549 cells treated with 10.0 ng·mL－1 CPP(0.251±0.012)was weaker than that in control group(0.928±0.016). Conclusion CPP can induce apoptosis in human lung cancer cell lines A549, and the probable mechanism is related to the down-regulation of survivin mRNA and protein.
  • Effects of Dihydromyricetin on Blood Lipid Metabolism and Antioxidation in Atherosclerosis Rats
    LIANG Zhudi,ZENG Xianbiao,WEI Guining,HE Fei,LYU Jihua,SU Hua,WEI Baowei
    2015, 34(6): 710-713. https://doi.org/10.3870/yydb.2015.06.002
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    Objective To observe the effect of dihydromyricetin of ampelopsis grossedentata on lipid metabolism and antioxidation in atherosclerosis (AS) rats. Methods Sixty Wistar rats were randomly divided into five groups (n=12 each): normal control group, model control group, positive control group, high dose dihydromyricetin group (40 mg·kg－1) and low dose dihydromyricetin group (10 mg·kg－1).Except normal control group, rats in the other groups were injected with a single dose of vitamin D3 (600 000 U·kg－1) and loaded with high fat diet to establish AS model.Simvastatin (5 mg·kg－1) was intragastrically administered to positive control group.High and low dose dihydromyricetin groups received intragastric administration of 40 and 10 mg·kg－1 dihydromyricetin, respectively.Equal volume of purified water was given to normal and model control group.After 24 weeks of administration, serum levels of lipids, activities of superoxide-dismutase (SOD) and malonicdialdehyde (MAD) were determined. Results As compared with model control group, triglycerides (TG) of high and low dose dihydromyricetin groups was decreased [(191.65±101.10) vs.(111.10±29.29) and (120.55±38.12) mg·L－1, respectively], total cholesterol (TC) was decreased [(151.64±33.62) vs.(148.49±30.14) and (118.90±27.38) mg·L－1], and high density lipoprotein cholesterol (HDL-C) was increased [(1.29±0.68) vs.(2.10±0.70) and (1.62±0.61) mmol·L－1], low density lipoprotein cholesterol (LDL-C) was decreased [(5.01±1.33) vs.(3.97±0.78) and (4.28±0.79) mmol·L－1], activity of SOD was increased [(141.03±42.52) vs.(187.97±42.08) and (150.99±46.17) U·mL－1], and MDA was decreased [(20.51±3.81) vs.(17.64±1.54) and (18.52±3.42) nmol·mL－1], with significant differences (P＜0.05, P＜0.01). Conclusion Dihydromyricetin can reduce the level of serum lipid, improve antioxidation activity, and has therapeutic effect for atherosclerosis.
  • Influence of Hesperidin on the Expression of TNF-α and IFN-γ in Concanavalin A-induced Acute Liver Injury in Mice
    YUAN Tingdong, CHEN Maojian, HUANG Wenjian, HE Yaqian, GONG Quan
    2015, 34(6): 714-717. https://doi.org/10.3870/yydb.2015.06.003
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    Objective To explore the protective effect of hesperidin pretreatment on concanavalin A (Con A)-induced acute liver injury and the effect on expression of TNF-α and IFN-γ. Methods Seventy-two SPF C57BL/6 mice were randomly divided into three groups: normal control group, model control group and hesperidin group.Acute liver injury model was established by injected with Con A.The hesperidin group was treated intragastrically with 1 000 mg·kg－1 hesperidin for 10 days.Model control group was treated intragastrically with the same volume of 0.5% of sodium carboxymethyl cellulose.Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured.Pathological changes in hepatic tissue were observed under microscope.The expression of TNF-α and IFN-γ mRNAs in hepatic tissue was measured by reverse transcription polymerase chain reaction (RT-PCR).The contents of TNF-α and IFN-γ in serum were detected by ELISA. Results Compared with model control group, the contents of ALT and AST in serum were significantly decreased (P＜0.01) in hesperidin group.Pathological changes in hepatic tissue were markedly improved.The expression of TNF-α and IFN-γ in the hepatic tissues and serum were significantly downregulated (P＜0.01).The concentrations of TNF-α and IFN-γ in hesperidin group were (717.05±205.22) and(611.06±92.82)pg·mL－1 in 2 h,(811.56±167.47)and(786.19±215.44)pg·mL－1 in 6 h.Compared with model control group, the expressions of TNF-α and IFN-γ in the hesperidin group were significantly downregulated (P＜0.01).But there was no significant difference between hesperidin group and model control group in 6 h after treated with Con A(P＞0.05). Conclusion Hesperidin pretreatment protects mice from Con A-induced acute liver injury possibly by inhibiting the expression of TNF-α and IFN-γ in the liver of mice.
  • Effect of Ganoderma Lucidum Polysaccharides Combined with Metformin on Oxidative Stress of Type 2 Diabetic Rats in Vivo
    QIAO Jin,DOU Zhihua,SHI Zhong,WU Feng,MENG Guoliang,CHEN Hui,ZHEN Huihua
    2015, 34(6): 718-721. https://doi.org/10.3870/yydb.2015.06.004
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    Objective To study the effect of ganoderma lucidum polysaccharides combined with metformin on oxidative stress of type 2 diabetic rats. Methods SD rats were fed with high fat diet for 4 weeks and injected with streptozotocin (30 mg·kg－1) to produce type 2 diabetic model.The diabetic rats were randomly divided into diabetes model group, ganoderma lucidum polysaccharides group (600 mg·kg－1), metformin group (600 mg·kg－1), combination group (ganoderma lucidum polysaccharides 300 mg·kg－1 ＋ metformin 300 mg·kg－1), After 12 weeks of treatment, the level of fasting blood glucose was determined, and the activity of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GSH-Px), total cholesterol (TC) and triglyceride (TG) were detected. Results The levels of fasting blood glucose in the treatment groups were significantly lower than that in the diabetes model group (P＜0.01).Furthermore, fasting blood glucose in the combination group was significantly lower than that in ganoderma lucidum polysaccharides group and metformin group (P＜0.01).Compared with diabetes model group, serum TC and TG in the treatment groups were significantly lower (P＜0.05, P＜0.01).Serum TC and TG were significantly lower in the combination group than in ganoderma lucidum polysaccharides group and metformin group (P＜0.05, P＜0.01).Compared with diabetes model group, serum SOD levels in the treatment groups were significantly higher (P＜0.01).Compared with ganoderma lucidum polysaccharides group and metformin group, serum SOD levels in the combination group was significantly higher (P＜0.05).Compared with diabetes group, serum MDA levels in the treatment groups were significantly lower (P＜0.01).Serum MDA in the combination group was significantly lower than that in ganoderma lucidum polysaccharides group and metformin group (P＜0.05).Compared with diabetes model group, serum CAT and GSH-Px in the treatment groups were significantly higher (P＜0.05, P＜0.01).Serum CAT and GSH-Px in the combination group were significantly higher than those in ganoderma lucidum polysaccharides group and metformin group (P＜0.05). Conclusion Ganoderma lucidum polysaccharides combined with metformin could effectively inhibit oxidantion stress in type 2 diabetic rats.The effect was better than ganoderma lucidum polysaccharides or metformin used alone.The possible mechanism may be related to increased activity of SOD, CAT, GSH-Px in vivo and regulation of dyslipidemia.
  • Protection of the Extracts of Walnut kernel on Learning and Memory Abilities and Hippocampal Neuron in Rat Model of Alzheimer's Disease
    FU Aini,ZHU Shuxiu,AI Yongxun,FU Qiang
    2015, 34(6): 722-725. https://doi.org/10.3870/yydb.2015.06.005
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    Objective To compare the influence of three kinds of Walnut kernel extracts on learning and memory ability as well as ultrastructural pathology in hippocampus of Alzheimer's disease (AD) rats. Methods AD rat model was established by injection of amyloid-beta protein (Aβ1-40) into the nucleus basalis of meynert.The AD rats were randomly divided into Walnut kernel-water group,Walnut kernel-ethanol group,Walnut kernel-acetone group,and the model control group,10 rats in each group.In addition,10 rats of normal control group and 10 rats of sham operation group were selected.The model control group was not treated; the treatment groups were intragastrically given Walnut kernel water extract,ethanol extract,and acetone extract (the equivalent of pharmacognosy 0.3 g·mL－1),respectively,dose 3 g·kg－1.The learning and memory ability was studied by Morris water maze,and ultrastructure of neurons was observed under the transmission electron microscopy. Results The time of looking for platform in Walnut kernel-water group,Walnut kernel-ethanol group,and Walnut kernel-acetone group were dropped swiftly at the beginning of 3 days, the third day is (51.80±4.37),(61.20±4.67),and (59.63±5.24),respectively; the model control group is (67.67±6.12) s.Compared with the model control group,the differences were significance (P＜0.05); However,the acetone extract of Walnut kernel can obviously enhance the learning and memory ability (P＜0.01),and the ultrastructure almost returned to normal. Conclusion The acetone extracts of Walnut kernel have the function of preventing Alzheimer's disease.
  • Inhibitory Effect of Paclitaxel Long-circulating Thermo-sensitive Liposome on Lewis Lung Cancer Cells in Mice
    LI Meibo, GU Shengjiu, ZHAO Wenpeng, ZHU Kaimei
    2015, 34(6): 726-729. https://doi.org/10.3870/yydb.2015.06.006
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    Objective To observe the effect of paclitaxel long-circulating thermo-sensitive liposome (PLTL) on inhibiting the growth of transplanting Lewis lung cancer cells in C57BL/6 mice. Methods The model of mice carried Lewis lung cancer was established.40 tumor-bearing mice were divided into five groups randomly: blank control group, model control group, PTX group, PTL group and PLTL group, 8 mice for each group.The blank control group and the model control group were injected with 0.9% sodium chloride solution.In PTX group, PTL group and PLTL group, the dose of per injection was calculated with the reference of PTX for 20 mg·kg－1, diluted with 0.9% sodium chloride solution, and the mice were injected via the tail vein with a volume of 0.2 mL each time.Except for the blank control group, 5 minutes after administration, the tumors of the other groups were subjected to local hyperthermia at (42±0.5) ℃ for 30 min.During the treatment period, transplantation tumor growth was observed; pathological morphology changes of tumor tissues and cells were detected by HE stain; apoptosis rate of tumor cells was determined by flow cytometry to investigate the inhibition effect of PLTL combined with local thermal therapy on the tumor. Results The inhibition rate of tumor in model control group, PTX group, PTL group and PLTL group was 21.81%, 48.87%, 57.22% and 78.87%, respectively.The apoptosis rate of tumor cells was (20.4±4.2)%, (42.7±3.8)%, (54.6±2.9)% and (69.7±5.0)%, respectively.Observed by pathology, apoptosis rate and necrosis number of tumor cells in PLTL group were significantly increased. Conclusion Compared with PTX and PTL, PLTL has an evident thermo-sensitive feature and can increase the anticancer effect of paclitaxel remarkably when combined with local hyperthermia.
  • Estimation of Cystatin C as a Marker of Renal Function to Predict Serum Trough Concentrations of Vancomycin
    LIAO Yimei,LIU Taotao,CHENG Daohai,LU Hua
    2015, 34(6): 730-732. https://doi.org/10.3870/yydb.2015.06.007
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    Objective To investigate the clinical efficiency of serum cystatin C as a marker of renal function to predict serum trough concentrations of vancomycin. Methods Data on trough concentrations of vancomycin(Cmea),serum creatinine(Scr)concentrations,cystatin C(Cys C)concentrations were collected from 81 hospitalized patients who received vancomycin therapy.Predicted vancomycin trough concentrations(Cpre)were calculated based on Scr or Cys C concentrations associating with Bayesian forecasting method through NONMEM software.Finally,the correlations and distinctions between Cpre and Cmea were comparatively analyzed. Results There were both correlation between Cpre and Cmea in relation to Scr or Cys C(r＝0.678,P＜0.01;r＝0.727,P＜0.01).Besides,the errors of mean predictive error(ME),mean absolute error(MAE)and root-mean-square error(RMSE)for Scr was －5.79,6.86,9.86 μg·mL－1,respectively,where as the errors for Cys C was －0.82,5.42,7.74 μg·mL－1,respectively. Conclusion Cystatin C is a good marker of renal function available for predicting serum vancomycin concentrations.
  • Effect of Chitosan Gel on the Rectum Injury Induced by Acetic Acid in Rats
    LI Xiaoping,HUANG Zonghai,HU Lichun,LOU Caixia
    2015, 34(6): 733-736. https://doi.org/10.3870/yydb.2015.06.008
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    Objective To detect the effect of chitosan gel on rectal injury which was induced by acetic acid. Methods 36 rats were anesthetized, 4% acetic acid swab was placed into the rectum (3 cm in depth for 1 min) to establish rectum injury model.These rats were divided into model control group, positive control group and drug group with 12 rats each group randomly.Chitosan gel was given to the drug group through anus with the dose of 1 575 mg·kg－1, and hemorrhoid cream (1 575 mg·kg－1) was administered in the positive control group for 6 successive days.At 30 min, 1, 4, 6 day(s) after administration, 3 rats of each group were sacrificed, and were dissected, rectal injury and rectal pathology morphology changing of each group at different time points of administration were compared through visual observation. Results At 30 min, 1, 4, and 6 day(s) after administrating chitosan gel, score of rectum mucosa injury in dury group was lower than those in model control and positive control group.Pathological changes (including epithelial tissue necrosis, mesenchyme hyperemia, hemorrhage, edema, gland injury, infiltration of inflammatory cells) were significantly slighter in drug group than in model control group and positive control group.Those lesions were restored faster in drug group than in model control group and positive control group, and there was no obvious scar. Conclusion Chitosan gel can obviously alleviate the rectal injury caused by 4% acetic acid and has therapeutic effect.
  • Vasodilation Effect of Extract of Jasminum samba
    LUAN Hairong, WANG Deli, HE Zhipeng, DAI Haibing, WU Hong
    2015, 34(6): 737-740. https://doi.org/10.3870/yydb.2015.06.009
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    Objective To observe the vasodilation effect of extract of Jasminum samba (EJs), a kind of traditional Chinese medicine, on ex vivo rat thoracic aortic rings, and to investigate its mechanism. Methods On ex vivo aortic ring perfusion device, influence of EJs on contraction of the aorta induced by phenylephrine (PE) or potassium chloride (KCl) were observed.Influence of N-nitro-L-arginine-methylester (L-NAME), barium chloride (BaCl2), glibenclamide (Gli) on vasodilating effect of EJs (0.5, 1, 2, 4, 8 g·L－1) were detected.Effect of EJs on the contraction of calcium chloride (CaCl2) and PE in Ca2＋-free medium were detected.[Ca2＋]i in vascular smooth muscle cells was determined by using laser scanning confocal microscope (LSCM). Results In blood vessels with intact endothelium, EJs concentration-dependently decreased PE- or KCl-induced vasoconstriction, the maximum dilating effect being (105.0±3.2)% and (78.0±6.5)%, respectively; L-NAME affected the vasodilatory effect of EJs on thoracic aorta rings (P＜0.01), the maximum dilatory effect being (58.0±6.9)%.BaCl2 and Gli had significant influence on vasodilation of EJs, and the contraction was obviously attenuated (P＜0.01), the maximum dilatory effect being (37.0±5.2)% and (78.0±10.0)%, respectively.EJs significantly inhibited contracting effect of PE on thoracic aorta rings in Ca2＋-free medium (P＜0.01).The maximum contraction effect was (70.0±6.3)%.EJs inhibited CaCl2-induced vasoconstriction (0.5－8 mmol·L－1), and vasoconstriction was decreased by (65.0±3.2)%.LSCM recorded that Fmax/F0 of 4 and 8 g·L－1 EJs was (2.0±0.2) and (1.5±0.2), respectively. Conclusion EJs exerted a dose-dependent vasodilating effect on rat isolated aorta rings.The mechanism might be related to promoting NO release, activating K＋ channels and decreasing the concentration of cytoplasmic Ca2＋.
  • Effect of Jieyuyubao Prescription on Endometrial Receptivity for the Chronic Stress Rats
    GENG Huizhuan, HE Yi, ZHOU Shunchang, WEI Wenshuang, FANG Honghao, LI Lierong
    2015, 34(6): 741-745. https://doi.org/10.3870/yydb.2015.06.010
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    Objective To study the effect of Jieyuyubao prescription on endometrial pinopodes and HOXa-10 mRNA in chronic stress rats , and to reveal the regulatory mechanisms of the endometrial receptivity. Methods Totally 48 SPF SD rats were randomly divided into three groups with weight stratified: normal control group (group A), model control group (group B) and experimental drug group (group C).Rat model of chronic stress was established in group B and group C.On the first day of stimulation, Jieyuyubao solution (1 g·mL－1) was intragastrically administered (11.8 mL·kg－1) to the rats of group C at 9:00 am, once daily, and stopped giving at the fourth day of pregnancy (pd4) after being confirmed by vaginal smears.Purified water (11.8 mL·kg－1) was intragastrically administered to the rats of group A and group B at 9:00 am.All the rats were mated at the sex ratio of female:male ＝2:1 at 16:00 pm on the 21st day.The rats were sacrificed at the pd4.Maturity of endometrium pinopodes was assessed under transmission electron microscope.HOXa-10 mRNA content was evaluated by real-time fluorescence-PCR. Results The maturity of pinopodes was as follows: the maturity score of normal control group, model control group and experimental drug group was (2.7±0.3), (1.3±0.3), and (2.1±0.2), respectively, with significant difference between experimental drug group and model control group (P＜0.05).HOXa-10 mRNA expression was significantly decreased in model control group (0.658±0.031) as compared with normal control group (0.965±0.102) (P＜0.05). Conclusion Jieyuyubao prescription maybe increase the number and maturity of pinopodes through promoting HOXa-10 mRNA expression in endometrial epithelium cells, and then improve endometrial receptivity.
  • Effect of Lipoic Acid Treatment on Endothelial Microparticles in Patients with Diabetic Peripheral Artery Disease in Lower Limbs
    DING Sheng, ZHAO Shi, WANG Zhongjing, ZHANG Xuyan
    2015, 34(6): 762-764. https://doi.org/10.3870/yydb.2015.06.017
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    Objective To evaluate the effect of lipoic acid on endothelial microparticles (EMP) in diabetic patients with peripheral artery disease (PAD) of the lower limbs. Methods Forty eight diabetic patients with PAD of the lower limbs were randomly divided into two groups: the therapeutic group and control group (each group has 24 cases).Patients of control group only received internal comprehensive medicine therapies for 3 weeks.Patients of therapeutic group were treated with both lipoic acid (600 mg lipoic acid injection in normal saline solution for intravenous drip, once daily for 3 weeks) and internal comprehensive medicine therapies at the same time.The levels of EMP in venous blood and Ankle-brachial index (ABI) were estimated before and after treatment by flow cytometry. Results Compared to the control group after treatment [(1 179.8±120.1) per μL], EMP value showed a significant reduction in therapeutic group [(1 032.4±117.2) per μL] (P＜0.01).The levels of ABI of therapeutic group (0.68±0.10) was increased obviously as compared that before lipoic acid treatment (P＜0.01).Patients treated with lipoic acid showed the higher levels of ABI than the control group after treatment (0.58±0.11) (P＜0.01). Conclusion Lipoic acid injection significantly decreased the level of EMP in diabetic patients with PAD of lower limbs.Lipoic acid might be an effective drug for diabetic patients with PAD.
  • Effects of Telmisartan and Valsartan on Insulin Resistance in Obese Hypertensive Patients
    WU Xinying, LI Jingjing, SONG Hongping, HAN Yong
    2015, 34(6): 765-767. https://doi.org/10.3870/yydb.2015.06.018
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    Objective To observe the effects of telmisartan and valsartan on insulin resistance in obese hypertensive patients. Methods Sixty-eight patients with essential hypertension and body mass index (BMI)≥25 kg·(m2)－1 were randomly divided into the telmisartan group (33 cases) and valsartan group (35 cases).Blood pressure, fasting serum glucose, fasting insulin (Fins) and insulin resistance (HOMA-IR) were measured before and after 16 weeks treatment. Results Compared with basal levels, SBP and DBP were significantly decreased in both group (P＜0.01).Fins and HOMA-IR were significantly decreased in telmisartan group [(12.9±2.5) mU·L－1 vs.(10.9±2.2) mU·L－1 (P＜0.01); (3.1±0.7) vs.(2.5±0.8) (P＜0.01), respectively], while no such changes were found in valsartan group [(13.3±4.7) mU·L－1 vs.(14.5±4.1) mU·L－1 (P＞0.05);( 3.2±1.4) vs. (3.2±1.0) (P＞0.05), respectively]. Conclusion In obese hypertensive patients, telmisartan and valsartan exert similar antihypertensive effect, but telmisartan may have a benefit in insulin resistance in comparison to valsartan.
  • Heteromorphism of Indomethacin Solvate and Characterization Method
    LI Yongliang, YANG Shiying, HU Kun, LYU Yang
    2015, 34(6): 785-790. https://doi.org/10.3870/yydb.2015.06.023
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    Objective To systematically study solvatomorphism of indomethacin and provide a scientific basis for the quality control of the solvated polymorphic impurities in this drug. Methods By changing the recrystallization solvent, solvent volume, recrystallization temperature, time and pressure, nine solvates and four non-solvated forms were discovered and prepared.The differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), X-ray powder diffraction (PXRD) and infrared spectrometry (IR) were introduced for characterization analysis.Furthermore, the test of influencing factors was used to explore the stability of solvated crystal form and the crystal transformation rules among them. Results Nine solvates were prepared, which including two solvates reported for the first time. Results showed that crystal forms of the nine solvates have different types or proportions of crystal solvents according to the various results of DSC, TGA, PXRD and IR.Moreover, the nine solvates prepared in this work were metastable crystal forms which could be transformed to non-medicinal forms. Conclusion The composition, thermodynamic property and transformation rule of all the solvates are elucidated in this work.In addition, an effective method for qualitative or quantitative analysis of these solvates was established.The standard graphs and data were used as basic data and scientific basis for the solvate control in the manufacturing of indomethacin.
  • Determination of (R,S)- Epigoitrin in Banlangen Granules by HPLC
    GONG Wei, LIU Zhongliang, ZHAO Qinghua, WEN Fenge, LI Peng,ZHANG Kun, ZHAO Mei
    2015, 34(6): 791-794. https://doi.org/10.3870/yydb.2015.06.024
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    Objective To establish HPLC method for determination of (R,S)-epigoitrin in Banlangen granules and discuss the content limitation. Methods The samples were separated on SHIMADZU VP-ODS (150 mm×4.6 mm, 5 μm) and the mobile phase consisted of methol-0.02% phosphoric acid solution (7:93) at a flow rate of 1.0 mL.min－1.The detection wavelength was set at 245 nm and the injection volume (in automatic sampler) was 10 μL.The content limitation was assessed according to the transfer rate and statistical data of the results. Results (R,S)-epigoitrin showed a good linear relationship at concentration of 0.058 7–150.349 5 μg·mL－1 (r＝0.999 7, n＝7).The average recovery rate was 98.72%, 98.40% and 98.60%, respectively; RSD was 1.84%, 0.50% and 1.82%, respectively.The content limitation of (R,S)-epigoitrin was unreasonable according to the transfer rate and the statistical data of the results. Conclusion The method is easy and simple to operate, accurate and stable in results, and highly specific, thus it is applicable for the quality control of Banlangen granules.The content limitation should be determined on the basis of pharmacokinetic and pharmacodynamic data.
  • Drug Loading and Release of Titanium Dioxide Nanoparticles with Near-infrared Light
    YING Jingyan, XU Airen, RONG Jianhui, MA Weicheng, HE Wenyue
    2015, 34(6): 795-798. https://doi.org/10.3870/yydb.2015.06.025
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    Objective To prepare titanium dioxide (TiO2) nanoparticles with good near-infrared light and study the loading and release of doxorubicin. Methods The Sm doped TiO2 nanoparticles (Sm-TiO2) were synthesized using a modified solvothermal reaction and then observed with transmission electron microscope.The fluorescence spectrum, doxorubicin loading capacity and release profile were also determined. Results The obtained Sm-TiO2 nanoparticles with the length from 100－200 nm were fusiform and well dispersed.The emission wavelength was 640－670 nm.The drug loading capacity in water was 11.5%.DOX in vitro was pH sensitive to release. Conclusion Sm-TiO2 nanoparticles have good near-infrared light, high drug loading capacity and controllable drug release are obtained and should be studied further more as a novel carrier.
  • Determination of S-isomer in Epinephrine Hydrochloride Injection by HPLC with Chiral Mobile Phase Additives
    JIN Li, ZHOU Jianli, ZHANG Xiaodan, GU Zhuoliang, BU Ying
    2015, 34(6): 799-8081. https://doi.org/10.3870/yydb.2015.06.026
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    Objective To establish a method for the chiral separation and determination of S-isomer in epinephrine hydrochloride injection by HPLC with chiral mobile phase additives. Methods Column of Purospher STAR RP-18 (4.6 mm×250 mm, 5 μm) was used.The mobile phase was acetonitrile-10 mmol·L－1 sodium dihydrogen phosphate buffer containing 10 mmol·L－1 sulfobutylether--cyclodextrin (pH adjusted to 3.0 with phosphoric acid) (98.5:1.5), detection wavelength was 280 nm, the flow rate was 0.8 mL·min－1, and the column temperature was 30 ℃. Results Good linear relationship was established between the peak area and the concentration of S-isomer over the range of 5.02－1501.50 μg·mL－1 (R2=0.999 7).The detection limit was 0.05 μg·mL－1. Conclusion The proposed method shows high repeatability and durability.It can be employed for the quality control of S-isomer in epinephrine hydrochloride injection.
  • Determination of Ceftazidime for Injection by HPLC
    LIU Yingqian, WU Qun, LUO Lijun,TANG Qian
    2015, 34(6): 802-804. https://doi.org/10.3870/yydb.2015.06.027
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    Objective To establish an HPLC method for the determination of ceftazidime for injection. Methods HPLC column was TSK-GEL G2000SWXL gel column.Mobile phase of phosphate buffer (pH 7.0) was 0.005 mol·L－1 disodium hydrogen phosphate solution-0.005 mol·L－1 sodium dihydrogen phosphate solution (61:39), the flow rate was 0.8 mL·min－1. The detection wavelength was 231 nm; column temperature was 30 ℃; the injection volume was 20 μL. Results Ceftazidime reference linear range was 0.51－25.64 μg·mL－1 (r＝0.999), ceftazidime for injection polymer linear range was 0.20－3.92 mg·mL－1 (r＝0.99), and the limit of quantification polymer was 0.71 μg. Conclusion The method is rapid and the separation was good.It can be used for the detection of ceftazidime for injection.
  • Quality Control of Dragon's Blood Spraying Film Agent
    LI Yihang, SONG Meifang,LYU Yana, LI Xuelan, CHEN Xi
    2015, 34(6): 805-807. https://doi.org/10.3870/yydb.2015.06.028
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    Objective To study the quality control methods for dragon's blood spraying film agent. Methods The pH value and viscosity of dragon's blood spraying film agent were detected.Drug dispersed homogeneous degree and particle sizes were determined with Nano Particle Size Analyzer and microscope.Content of Loureirin B was measured by Ultra Performance Liquid-Chromtography (UPLC).UPLC was performed on Waters BEH C18 column (2.1 mm×100 mm,1.7 μm), the wavelength was 280 nm, the column temperature was 40 ℃, and the mobile phase was 0.1% formic acid aqueous solution and acetonitrile, and the flow rate was 0.8 mL·min－1. Results The pH value and viscosity of dragon's blood spraying film agent were stable, drug dispersion was homogeneous, and particle size of the drug was tiny.The concentration of Loureirin B had a good linear relationship in the range of 15.51－77.54 μg. Conclusion This method can be accurately controlled, has good stability and repeatability, and can fully control quality of dragon's blood spraying film agent.
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