利拉鲁肽前体肽GLP-1(7-37)K34R在大肠埃希菌中的表达与优化*

李朋彦,周成慧,李潜,赵伟,罗学刚

Expression and Optimizated of Liraglutide Precursor Peptide,GLP-1(7-37)K34R,in Escherichia coli

Pengyan LI,Chenghui ZHOU,Qian LI,Wei ZHAO,Xuegang LUO

图2 不同标签融合蛋白的表达 A.pGEX-6p-3-GLP1a;M.分子量标记;1.诱导pGEX-6p-3;2.诱导pGEX-6p-3-GLP1a;3.裂解后上清液;4.裂解后沉淀。B.pET28a-Ub-GLP1a;Lane M.分子量标记;1.诱导pET28a;2.诱导pET28a-Ub-GLP1a;3.裂解后上清液;4.裂解后沉淀。C.pE-SUMO-GLP1a;M.分子量标记;1.诱导 pE-SUMO;2.诱导pE-SUMO-GLP1a;3.裂解后上清液;4.裂解后沉淀。

Fig.2 Expression of different tag fusion proteins A.pGEX-6p-3-GLP1a;M.molecular weight marker;1.induced pGEX-6p-3;2.induced pGEX-6p-3-GLP1a;3.supernatant after cracking;4.precipitation after cracking.B.pET28a-Ub-GLP1a;M.molecular weight marker;1.induced pET28a;2.induced pet28a-ub-glp1a;3.supernatant after cracking;4.precipitation after cracking.C. pE-SUMO-GLP1a;M.molecular weight marker;1.induced pE-SUMO;2.induced pE-SUMO-GLP1a;3.supernatant after cracking;4.precipitation after cracking.