1.Department of Pharmacy, Shanghai Jiaotong University Affiliated the Sixth People's Hospital, Shanghai 200233,China 2.Department of Pharmacy, Shanghai University of Medicine & Health Sciences Affiliated the Sixth People's Hospital East Campus, Shanghai 201306,China 3.College of Food Sciences & Technology, Shanghai Ocean University, Shanghai 201306,China 4.College of Pharmacy, California North State University, California CA95757, USA
Objective To explore the mechanism of Xiaoaiping injection enhancing the inhibitory effect of paclitaxel on the proliferation of ovarian cancer cell A2780. Methods Ovarian cancer cell line A2780 were treated by Xiaoaiping injection (20,40,80,160 mg·mL-1) or paclitaxel(10,40,80,160 nmol·L-1) or Xiaoaiping injection combined with paclitaxel, respectively. The inhibition ratios of different drugs on the proliferation of A2780 cells were measured by MTT method. The changes in cell morphology after drug treatment were monitored by microscope. The mRNA expression of PXR, CAR and CYP2C8, CYP3A4, CYP3A5, transporter P-gp were detected by RT-PCR. The protein expression of PXR, CYP2C8 were measured by Western blotting. ResultsXiaoaiping injection inhibits the proliferation of A2780 cells in a time- and concentration-dependent manner. Compared with paclitaxel treatment alone, Xiaoaiping injection combined with paclitaxel exhibit more significant inhibitory effect on A2780 cell proliferation RT-PCR and Western blotting results revealed that Xiaoaiping injection can significantly inhibit paclitaxel-induced over expression of PXR and CYP2C8. ConclusionXiaoaiping injection can significantly enhance the inhibitory effect of paclitaxel on the proliferation of ovarian cancer A2780 cells. The mechanism may be associated with the inhibitory effect of Xiaoaiping on paclitaxel-induced overexpression of nuclear receptor PXR and its downstream drug metabolic enzyme CYP2C8.
张湘奇,陈君君,杨姣,石美智,祁美娟,肖霄,王洪斌,韩永龙. 消癌平注射液调节核受体增强紫杉醇抑制卵巢癌A2780细胞增殖的机制*[J]. 医药导报, 2019, 38(6): 693-700.
Xiangqi ZHANG,Junjun CHEN,Jiao YANG,Meizhi SHI,Meijuan QI,Xiao XIAO,Hongbin WANG,Yonglong HAN. Enhances of the Inhibitory Effect of Paclitaxel on the Proliferation of Ovarian Cancer A2780 Cell by Xiaoaiping Injection via Modulating Nuclear Receptors. Herald of Medicine, 2019, 38(6): 693-700.
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