1.Xinjiang Institute of Chinese Traditional Medical and Ethical Materia Medical, Urumqi 830002,China 2.Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University,Urumqi 830000, China
Objective To investigate the anti-inflammatory,antitussive and in vitro bacteriostatic effects of the extract of Anchusa italica Retz. ,so as to scientific basis for clinical application. Methods The anti-inflammatory effect of different extracts was observed by inflammation model induced by carrageen glue toes swelling of rats; Antitussive effect was observed by ammonia-induced cough model; The in vitro bacteriostasis effects of different extracts on 5 strains (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans) was evaluate by the tube dilution method . Results The swelling of rat toes was significantly inhibited at different time after inflammation by 0.63 g·kg-1, 2.52 g·kg-1 of ethanol extracts from Anchusa italica Retz., 2.52 g·kg-1 of ethyl acetate and n-butanol parts (P<0.01), and The inhibitory effect of 2.52 g·kg-1 ethanol extracts on inflammation was similar to that of dexamethasone group, but there was no statistically significant difference between n-butanol parts and ethanol parts; In the antitussive experiment in mice, 0.90 g·kg-1, 3.04 g·kg-1 ethanol extracts, and 3.04 g·kg-1 of the n-butanol extracts had better effect than the jizhi syrup in prolonging the incubation period of cough in mice (P<0.05 or P<0.01), and the parts of the 3.04 g·kg-1dose group could significantly reduced the frequency of cough (P<0.05); All extracts had antibacterial activity against Bacillus subtilis, and the inhibition levels of ethyl acetate and water soluble parts were stronger. Conclusion The ethanol extracts and n-butyl alcohol parts has anti-inflammatory and antitussive pharmacological activities, and ethyl acetate parts has inhibitory activity against bacillus subtilis in vitro.
徐晓琴,陈良,张娟,孙宇,卿德刚,倪慧. 意大利牛舌草提取物抗炎镇咳与体外抑菌作用*[J]. 医药导报, 2019, 38(6): 726-730.
Xiaoqin XU,Liang CHEN,Juan ZHANG,Yu SUN,Degang QING,Hui NI. Effects of Extracts of Anchusa italica Retz. on Anti-inflammatory,Cough-relieving and Bacteriostasis in Vitro. Herald of Medicine, 2019, 38(6): 726-730.