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  2006, Vol. 25 Issue (1): 0-0    
  药物研究 本期目录 | 过刊浏览 | 高级检索 |
板蓝根有效部位F022对脂多糖刺激小鼠组织膜结构伸展刺突蛋白mRNA表达的影响
刘云海,谢委,方建国,李敬
华中科技大学同济医学院附属同济医院药学部,武汉 430030
Effect of Component F022 in Radix Isatidis on the Expression Upregulation of Moesin mRNA in Mice Induced by Lipopolysaccharide
LIU Yunhai;XIE Wei;FANG Jianguo;LI Jing
(Department of Pharmacy, Tongji Hospital Affiliated with Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China)
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摘要 目的探讨板蓝根抗内毒素活性部位F022对脂多糖(LPS)刺激小鼠组织膜结构伸展刺突蛋白mRNA(moesin mRNA)表达的影响。 方法实验前1周用卡介苗腹腔注射小鼠0.2 mL·(20 g)-1,30只小鼠平分为5组,药物实验组分别灌胃给予1.00%,0.50%和0.25%F022液0.4 mL·(20 g)-1,阴性对照组灌胃给予1.00%F022液0.4 mL·(20 g)-1,LPS模型组灌胃给予同等量0.9%氯化钠溶液。1.5 h后重复给药1次。第2次给药后30 min,药物实验组、LPS模型组小鼠尾静脉注射LPS 0.2 mL·(20 g)-1,9 h后小鼠乙醚麻醉,取肝、脾、肾组织作moesin mRNA原位杂交处理,显微镜下观察胞浆被染色情况。 结果组织胞浆着色呈棕黄色者为阳性。LPS可致小鼠肝、肾、脾组织moesin mRNA表达增加,而预先给予板蓝根F022部位者moesin mRNA表达被抑制,且有剂量依赖特征。结论板蓝根F022部位对脂多糖致小鼠肝、肾、脾组织moesin mRNA表达有抑制作用。
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关键词 板蓝根内毒素脂多糖膜伸展刺突蛋白mRNA    
AbstractObjectiveTo probe into the effect of the antiendotoxic part in Radix Isatidis on the expression of moesin mRNA in tissues of mice induced by lipopolysaccharide(LPS).MethodsOne week before the test, an intraperitoneal injection of 0.2 mL·(20 g)-1 bacillus calmettcguerin(BCG) was given to each of 30 mice, which were then randomized equally into 5 groups. Mice of the test groups were given 0.4 mL·(20 g)-1 of 1.00%,0.50% and 0.25% solution of F022 by gastrogavage , mice of the control group were given 0.4 mL·(20 g)-1 of 1.00% solution of F022 by gastrogavage , and mice of the model group were given each an equal volume of 0.9% sodium chloride. The above procedures were repeated 1.5 h later, and 30 min following the second drug administration, the test groups and the model group were given 0.2 mL·(20 g)-1 of LPS. Mice were anesthetized 9 h later by ether with the liver, kidney and spleen tissues treated by moesin mRNA hybridization in Situ and the staining of cytoplasm observed under microscope. ResultsA brown yellow staining of the tissue cytoplasm was taken as positive, and the administration of LPS could increase moesin mRNA expression .With prior administration of F022 part in radix isatidis the moesin mRNA expression by LPS was inhibited with a dose dependent characteristic. ConclusionThe F022 part in radix isatidis can inhibit the LPS induced moesin mRNA expression in tissues of mice liver, kidney and spleen with an antiendotoxic mechanism possibly at the molecular level.
收稿日期: 1900-01-01      出版日期: 2006-01-01
通讯作者: 刘云海   
引用本文:   
刘云海;谢委;方建国;李敬. 板蓝根有效部位F022对脂多糖刺激小鼠组织膜结构伸展刺突蛋白mRNA表达的影响[J]. , 2006, 25(1): 0-0.
LIU Yunhai;XIE Wei;FANG Jianguo;LI Jing. Effect of Component F022 in Radix Isatidis on the Expression Upregulation of Moesin mRNA in Mice Induced by Lipopolysaccharide. , 2006, 25(1): 0-0.
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