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• 01 September 2013 Volume 32 Issue 9

    

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  药物研究
  特约稿
  药物研究
  妇产科用药专栏
  药物与临床
  药物制剂与药品质量控制
  用药指南
  

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药物研究
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  药物研究

  In Vitro Evaluation of Chito-oligosaccharides as Gene Vectors

  JIN Chunyang;ZHAO Tianqin;FANG Liang;MOU Mingwei;HAO Xin-bao

  2013, 32(9): 1115-1119. https://doi.org/10.3870/yydb.2013.09.001

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  ABSTRACT Objective To evaluate the physicochemical property of chitooligosaccharides and the activity of the transfected 293T cells.MethodsChitooligosaccharides of small relative molecular mass was used in our study. The cytotoxicity of different chitooligosaccharides was measured by MTT. The capability of chitooligosaccharides binding and protecting plasmid DNA was detected with agarose gel electrophoresis. The result of transfection was observed under fluorescence microscope. ResultsOn the second day, high concentration of chitooligosaccharides demonstrated little toxicity. On the fourth day, the cell survival rate of all experimental groups was higher than that of the control group. Chitooligosaccharides exhibited complete binding with all DNA and effective protection when the mass ratio was over 20. Green fluorescence was found after transfection. ConclusionChitooligosaccharides was used as gene verors to transfect 293T cells.
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  药物研究

  In Vitro Evaluation of Chitooligosaccharides as Gene Vectors

  JIN Chunyang;ZHAO Tianqin;FANG Liang;MOU Mingwei;HAO Xin-bao

  2013, 32(9): 1119-1121. https://doi.org/10.3870/yydb.2013.09.002

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  ABSTRACT Objective To evaluate the physicochemical property of chitooligosaccharides and the activity of the transfected 293T cells.MethodsChitooligosaccharides of small relative molecular mass was used in our study. The cytotoxicity of different chitooligosaccharides was measured by MTT. The capability of chitooligosaccharides binding and protecting plasmid DNA was detected with agarose gel electrophoresis. The result of transfection was observed under fluorescence microscope. ResultsOn the second day, high concentration of chitooligosaccharides demonstrated little toxicity. On the fourth day, the cell survival rate of all experimental groups was higher than that of the control group. Chitooligosaccharides exhibited complete binding with all DNA and effective protection when the mass ratio was over 20. Green fluorescence was found after transfection. ConclusionChitooligosaccharides was used as gene verors to transfect 293T cells.
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  药物研究

  Effects of 3’daidzein Sulfonate Sodium on Bone Metabolism of Ovariectomized Rats

  SHI Lang;DU Bing;ZHANG Tingting;JIN Lan;GU Yuanyuan;CHEN Dazhong

  2013, 32(9): 1122-1124. https://doi.org/10.3870/yydb.2013.09.003

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  ABSTRACTObjectiveTo investigate the effects of 3’daidzein sulfonate sodium on bone metabolism of ovariectomized rats. MethodsSixty female SD rats were selected and randomly divided into six groups：shamoperation （normal control）, ovariectomized model （model）, ovariectomized with nilestriol （positive control）, highdose, middledose, and lowdose （ovariectomized with 3’daidzein sulfonate sodium 100, 50 and 25 μg•kg－1 ,respectively）. After continuous administration for 6 weeks and 24 h after the last administration, the serum was obtained after anesthesia and contents of the osteocalcin （BGP）, alkaline phosphatase（AKP）, tartrateresistant acid phosphatase （STrACP）, and progesterone were determined. ResultsIn the model control and high dose groups, respectively, the BGP concentration was （5.04±0.05） and （4.82±0.05） ng•mL－1, AKP was （47.31±0.02） ×10－2and （36.90±0.21） ×10－2 U•mL－1, STrACP was （1.72±0.11） and （3.02±0.13） U•L－1, and progesterone was （49.72±2.00） and （53.41±1.12） ng•mL－1. 3’daidzein sulfonate sodium decreased the content of serum osteocalcin and AKP significantly （P＜0.01 ）, but elevated STrACP （P＜0.05） markedly and increased the content of progesterone （P＜0.01）. Conclusion3’ daidzein sulfonate sodium regulates bone metabolism by promoting bone formation.
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  药物研究

  开心散对失眠模型大鼠睡眠周期的影响

  XU Yaji;ZHANG Xu;GUO Wenjie;GU Tianming

  2013, 32(9): 1124-1126.

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  ABSTRACTObjectiveTo study the effect of kaixin powder on the sleep cycle of insomnic rats. MethodsThe insomnic rat model was established with a intermittent current stimulation. The rats were randomly divided into six groups, the negative control, insomniac control, diazepam control （4 mg•kg－1）, low, middle, high doses of kaixin （2，4，8 g•kg－1） powder groups. The mice were orally administered with the drugs or vehicle for seven consecutive days, and the effect of kaixin powder on the sleep cycle of insomnic mice was studied with electrocorticography. ResultsThe wake time in the mid and high dose groups were （229.6±11.5） and （211.4±13.1） min, respectively, which was improved compared with the negative control group （277.6±19.3） min. The total sleep time （TST） in the mid ［（250.4±32.6） min］and high ［（268.6±29.7） min］ dose groups were also improved from the negative control group ［（202.4±23.5） min］. The improvement effects of kaixin powder were especially obvious for the SWS2 and REMS, which were superior to the diazepam positive control. ConclusionKaixin powder can improve sleep of insomnic rats by extending SWS2 and REMS.
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  药物研究

  Percutaneous Permeability of the Volatile Oil of Cyperus Rotundus and Promotion for Transdermal Effect of Acetaminophen

  LIU Nannan;GUO Shuyun;WANG Qingwei;CHANG Min

  2013, 32(9): 1127-1130.

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  Effects of Dexmedetomidine Pretreatment on Neonatal Rat Cardiomyocyte Hypoxia/Reoxygenationinduced Energy Metabolic Disturbance

  CHEN Jianing;ZHANG Junfeng;WANG Tao

  2013, 32(9): 1130-1133.

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  ABSTRACTObjectiveTo study the effects of dexmedetomidine （DEX） on hypoxia/reoxygenation （H/R）induced energy metabolic disturbance in neonatal cardiomyocyte. MethodsThe H/R model was established in cardiomyocytes of neonatal rats cultured for one day. Four groups were assigned as control, H/R model, pretreatment with DEX at low dosage（0.1 μmol•L－1） and high dose（1.0 μmol•L－1）. The activities of lactic dehydrogenas （LDH）, ATPase, mitochondrion succinic dehydrogenase （MSDH） and mitochondrion membrane potential were measured. Cell apoptosis rate was detected by flow cytometry. ResultsThe LDH activity of the control, H/R and DEX at 0.1 μmol•L－1 groups was （336.32±55.98）,（992.72±100.78），and （716.26±78.22） U•L－1 ,respectively; the A value of MSDH at 570 nm was 1.12±0.22, 0.59±0.11, and 0.76±0.09, respectively; the mitochondrion membrane potential （relative magnitude） was 879.22±90.53，623.18±70.89，and 682.78±80.56, respectively. Compared with the control, the levels of ATP, ADP and AMP were downregulated in H/R group. The activities of LDH, MSDH and mitochondrion membrane potential were significantly lowered, the apoptosis rate in H/R group was elevated. ConclusionDexmedetomidine can alleviate H/R injury in neonatal cardiomyocyte，which may be associated with the down regulation of apoptosis.
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  药物研究

  Corneal Retention and Irritation Behavior of Azithromycin Ophthalmic Gel

  SHI Zhao-hui;CHANG Xiaohui;YANG Lei;JIANG Weihua;HAN Xiao;YE Huizhu;WANG Yan;WANG Dongkai

  2013, 32(9): 1134-1138.

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  ABSTRACTObjectiveTo investigate corneal retention and irritation behavior of azithromycin in situ gel for ophthalmic drug delivery.MethodsThe γscintigraphy was used to evaluate the residence time of the preparation in rabbit eyes，using radiant intensity as the indicator.Draize method was employed to study the irritation behavior of azithromycin in ophthalmic gel through single and multiple doses.ResultsThe corneal retention of azithromycin ophthalmic gel was enhanced significantly and the residence time was prolonged 2.5 times compared with that of ordinary eye drops.The irritation tests showed an excellent biocompatibility of azithromycin ophthalmic gel.ConclusionThe azithromycin in situ gel exhibites not only little corneal irritation but also longer residence time，which is worthy of exploitation.
特约稿
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  特约稿

  Detection of Metalloenzyme IMP4 in Carbapenemresistant Enterobacter Aerogenes

  SHI Dong-yan;YANG Jing;ZHAO Jianhong

  2013, 32(9): 1139-1141.

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  ABSTRACTObjectiveTo investigate the resistance mechanism of carbapenemresistant enterobacter aerogenes.MethodsDrug resistance was determinated by KB test and Etest. The types of enzyme production were detected by modified Hodge test and metalloenzyme test．PCR was used to test and sequence drug resistance genes． The conjugation experiment was applied to conjugating and transferring genetic positive strains.ResultsThe results of modified Hodge test and metalloenzyme test were positive. The amplified results of IMP,CTXM, and IntI genes were positive and sequence analysis revealed IMP4 and CTXM15．Ompk36 gene was tested negative. ConclusionThe mechanism of drug resistance of carbapenemresistant enterobacter aerogenes involves IMP4 and CTXM15 production and lacking of outer membrane protein at the same time.
药物研究
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  药物研究

  Determination of Ginkoglide B in Plasma of Rats by Gas Chromatography-Mass Spectrometry

  ZANG Cheng;TIAN Yichao;XU Lingyun

  2013, 32(9): 1142-1145.

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  ABSTRACTObjectiveTo develop a gas chromatographymass spectrometry （GCMS） method for determining ginkoglide B in rat plasma. MethodsUsing ginkoglide A as an internal standard, the plasma was extracted with diethyl etherand derived by diethyl ether and added with （BSTFA）/ （TMCS）（99：1）, and the content of ginkoglide B was assayed by GCMS. ResultsA good linearity of ginkoglide B was presented within 0.02－8.00 μg•mL－1, the lowest limit of quantification was 0.02 μg•mL－1, and interday and intraday relative standard deviation （RSD） was 1.30%－5.45%and 5.58%－6.03%, respectively. The recovery of ginkoglide B was 93.3%－103.7%. ConclusionThis method is simple, sensitive and specific, which is applied to pharmacokinetics study of ginkoglide B.
妇产科用药专栏
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  妇产科用药专栏

  Seventyfour Cases of Cervical Cancer Treated with Paclitaxel Liposome Combined with Nedaplatin

  YAN Lin;HUANG Lei;ZHANG Qinghua

  2013, 32(9): 1146-1148.

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  ABSTRACTObjectiveTo evaluate the efficacy and toxicity of paclitaxel liposome plus nedaplatin combined with laparoscopic hysterectomy under + pelvic lymphadenectomy in the treatment of advanced cervical cancer. MethodsA total of 74 patients with Ⅰb2 and Ⅱ b cervical cancer were treated by infusing with paclitaxel liposome155 mg•（m2）－1 and nedaplatin 80 mg•（m2）－1 for two 21day cycles before surgery. Patients were evaluated for response to treatment. ResultsEighteen patients showed complete response（CR），38 showed partial response （PR），16 showed stable disease （SD），and 2 showed progress disease（PD）．The effective rate was 75.7%. The main side effects were neutropenia，thromboeytopenia，nausea，vomiting and hair loss, which were generally tolerable after symptomatic treatment. Efficient neoadjuvant chemotherapy combined with laparoscopic radical hysterectomy + pelvic lymphadenectomy was successful for treating advanced cervical cancer; the postoperative recovery was well. ConclusionPaclitaxel liposome plus nedaplatin can effectively reduce the local infiltration of tumor , adhesions difficulty of operation. The side effects are tolerable. The treatment is worth of promotion and application.
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  妇产科用药专栏

  Combination of Aspirin and Heparin in the Treatment of Recurrent Spontaneous Abortion Associated with Antiphospholipid Antibodypositive：A Meta Analysis

  CHEN Chaoli;ZHANG Yuanzhen;LI Jiafu;REN Liang

  2013, 32(9): 1149-1155.

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  ABSTRACTObjectiveTo assess the effect of combination of heparin and aspirin and aspirin alone in the treatment of recurrent spontaneous abortion associated with antiphospholipid antibodypositive.MethodsThe total of 93 articles were screened, analyzed by Review Manager 5.0, calculating the odds ratio （OR） and 95%confidence intervals （95%CI） and drawing forest plots. Then the sensitivity analysis was performed and publication bias was evaluated.ResultsFive randomized controlled trials were included. Combination group （aspirin plus heparin） could increase the birth rate ［OR = 2.28, 95%CI （1.24, 4.18）, P＜0.05］ when compared to the aspirin alone, which showed significant difference. The combination of heparin and aspirin had no significance effect on the birth weight, preterm birth rate, preeclampsia, fetal growth restriction, minor bleeding and cesarean section rate. ConclusionCombination of heparin and aspirin is better than aspirin alone in the treatment of recurrent spontaneous abortion accompany with antiphospholipid antibodypositive.
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  Effect and Safety Analysis of Labor Induction with Dinoprostone During Different Session for Premature Rupture of Membranes

  MAO Yanhong;HUANG Fenghua;LI Jiafu

  2013, 32(9): 1156-1159.

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  ABSTRACTObjectiveTo evaluate the efficacy and security of labor induction with dinoprostone suppository during different time after premature rupture of membranes. MethodsThe total of 78 pregnant women who had premature rupture of membranes, with cervical Bishop scores ≤6, without labor induction and drug contraindication, were divided into two groups：group A with 42 women, which applied with one piece of dinoprostone （10 mg） in 12 hours after the premature rupture of membrane; group B with 36 women, which applied with one piece of dinoprostone （10 mg） in 24 hours after the premature rupture of membranes. The effect of labor induction with dinoprostone suppository in two groups was compared, as well as mode of delivery, cesarean section rate, neonatal and maternal morbidity and other clinical indicators.ResultsThe success and effective rate of labor induction were 92.9%and 97.6%in group A, and 75.0%, 83.3%, respectively in group B. The cesarean section rates was 28.6%in group A, and 83.3%in group B. These differences between two groups were statistically significant （P＜0.05）. There were no significant difference in the puerperal infection rate, postpartum hemorrhage and laceration rate in two groups. There were no neonatal asphyxia and postpartum hemorrhage in two groups. ConclusionLabor induction with dinoprostone in 12 h after premature rupture of membranes is safe and effective, which could reduce the cesarean section rate and don’t increase severe maternal and fetal risk.
药物与临床
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  药物与临床

  Clinical Observation of CVP Followed by CHOP Regimen for Treating Advancedstage Diffusing Large Bcell Lymphoma

  HUANG Yanjing;CHEN Junmin;WANG Haixia;CHEN Denglin;XIE Xianhe

  2013, 32(9): 1166-1169.

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  ABSTRACTObjectiveTo evaluate the efficacy and adverse effects of CVP followed by CHOP and CHOP regimens in the treatment of advancedstage diffusing large Bcell lymphoma（DLBCL）. MethodsAccording to clinical assessments, a total of 25 patients with advancedstage DLBCL were divided into two groups. Group A （n＝10 , high chemotherapy risks）was treated with CVP followed by CHOP chemotherapy, group B （n＝15）was treated with CHOP chemotherapy. The efficacy and adverse effects of two groups were evaluated after 68 cycles chemotherapy. ResultsThe response cases of group A and group B were 7 and 11 cases, respectively. There were two cases of grade IV leukopenia in group B, and thus the drug dosage was adjusted and treatment was prolonged. The incidence of adverse effects showed no significant difference between two groups. After 1year followup, two groups had similar survival patterns. ConclusionCVP followed by CHOP protocol shows equivalent clinical efficacy as CHOP protocol, but with better toleration. This protocol could be chosen for high chemotherapy risk patients with advancedstage DLBCL.
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  Effect of Enalapril on the Atrial Natriuretic Peptide and Atrial Remodeling in Patients with Paroxysmal Atrial Fibrillation

  TIAN Liqun;ZHENG Qiongli;HE Liqun;Li Zuomin

  2013, 32(9): 1170-1173.

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  ABSTRACTObjectiveTo explore effects of enalapril on atrial natriuretic peptide （ANP） and atrial remodeling in patients with paroxysmal atrial fibrillation （PAF）. MethodsA total of 91 PAF patients were divided into control （n＝55） and treatment （n＝36） groups. All the patients received conventional treatment and amiodarone 200 mg tid for 5－7 days, with a dose reduction to 200 mg bid for another 5－7 days, followed by a maintenance dose of 200 mg daily or every other day. Enalapril 10 mg daily was added for patients in the treatment group, with a dose reduction to 5 mg daily in case of hypotension. The changes in the plasma level of ANP and ultrasonic parameters such as left atrial diameter （LAD）, left ventricular enddiastolic dimension （LVEDD） and left atrial volume at the largest area （LAVmax） at the baseline and after 3 and 6 months of followup were observed. Furthermore, the alteration in the reoccurrence of atrial fibrillation after 3 and 6 months of followup was also examined.ResultsCompared with the controls, the plasma level of ANP［（159.8±67.9） versus （171.5±74.7） pg•mL－1 at 3 months, （143.6±57.4） versus （174.1±63.5） pg•mL－1 at 6 months; P＜0.01］ and LAVmax ［（85.6±27.8） versus （96.9±26.4） mL•（m2）－1 at 3 months, （93.3±24.7） versus （108.7±28.0） mL•（m2）－1 at 6 months; P＜0.05］ after the treatment of enalapril were significantly decreased, so did the LAD at 6 months after the treatment of enalapril ［（34.6±4.9） versus （40.5±4.6） mm, P＜0.05）］. There were no significant differences in LAD after 3 months of followup and LVEDD after either 3 or 6 months of followup. The patients with PAF in the enalapril group had significantly fewer reoccurrence rate compared to the control group （0.17 versus 0.39 at 3 months; 0.33 versus 0.60 at 6 months, P＜0.05）. ConclusionEnalapril can decrease the plasma level of ANP, improve left atrial remodeling and prevent the reoccurrence of atrial fibrillation in patients with PAF.
药物制剂与药品质量控制
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  药物制剂与药品质量控制

  Content Determination of the Main Chemical Constituents in the Effective AntiPRRSV Fraction of the Branches and Leaves of Lonicera japonica

  LIU Liqing;DENG Zhangshuang;YANG Jin;CHEN Jianfeng;ZOU Kun;TAI Yuanlin;HU Jianzhong

  2013, 32(9): 1203-1206.

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  ABSTRACTObjectiveTo establish a method for content determination of 4 chemical constituents,including benzyl alcohol O（6’OβDxylopyranosyl）βDglucopyranoside（1）, gentiopicrin（2）, dihydrodehydrodiconiferyl alcohol 4’OβDglucoside（3）,and apigenin 5OβDglucopyranoside（4）,in the effective anti porcine reproductie and respiratou syndrome virus （PRRSV） fraction of the branches and leaves of Lonicera japonica by HPLC. MethodsThe HPLC separation was performed on Acclaim C18 （4.6 mm×250 mm，5 μm） column at 30 ℃.The mobile phase in the gradient elution was acetonitrilewater（10：90－30：70）, at the flow rate of 1.0 mL•min－1 and the detection wavelength of 203,210,254 ,and 280 nm, respectively. ResultsThe average recovery of the four ingredients was 98.96%（RSD=1.49%）, 98.73%（RSD=1.42%）, 98.93%（RSD=0.98%）, and 99.14%（RSD=0.96%） ,respectively.ConclusionThe method is simple, accurate and reproducible,which can be used in the content determination of the four chemical constituents in the effective antiPRRSV fraction of the branches and leaves of Lonicera japonica.
用药指南
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  Investigation of Antibiotic Therapy in Eradication of Helicobacter pylori

  2013, 32(9): 1234-1236.

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