中国科技论文统计源期刊　中文核心期刊  
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日本科学技术振兴机构数据库（JST）
第七届湖北十大名刊提名奖  

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    药物研究
  • 药物研究
    Impact of Guanxinping Granules on Carotid Atherosclerosis Plaque and Blood Lipid in Mesocricitus Auratus
    ZHU Xuanxuan;LI Qiyi;WU Xutong;WANG Haidan
    2012, 31(2): 135-138.
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    ObjectiveTo study the effects of guanxinping granules on carotid atherosclerosis plaque and blood lipid in mesocricitus auratus. MethodsFed mesocricitus auratus with high fat diet to establish a model of atherosclerosis(AS)，with normal diet for the normal group. After four weeks，the AS rats were divided into model group，normal control group， low dose group，and high dose group. Rats were administered for 8 weeks. By the ends of 4 week and 8 week， cholesterol (TC)，triglyceride (TG)，low density lipoprotein cholesterol (LDLC) of serum were detected. The coronary artery and hepatic tissue were tested by using HE staining. ResultsCompared with the normal group， TC，TG，LDLC of model group significant raised（P＜0.01）. Guanxinping granules decreased the level of TC，TG， LDLC，compared with the model group (P＜0.05，P＜0.01). The diffuse degeneration of liver cells and lipid deposition in aortic wall were found in the model group which mainly manifested as hyperlipidemia. And scattered acicular or foamy voids，elastic fiber breakage，and local endothelial cell defect occurred in wall full layer. After intervention with guanxinping granules for 8 weeks, lesions were significantly reduced compared with that in the model group，no active vascular wall diffuse and lipid deposition appeared. But the wall full layer was still scattered with acicular or foamy voids and elastic fiber breakage. ConclusionGuanxinping granules effectively regulate lipid metabolism，delay or reverse coronary atherosclerotic process, which protect vascular endothelial cells and improve endothelial dysfunction function.
  • 药物研究
    Effects of Oxymatrine Injection on Expression of NOD2 and TNFα in Colonic Intestinal Mucosa of Rats with Experimental Colitis
    TANG Qing;FAN Heng;HU Hui;SHOU Zhexing;LIU Xingxing
    2012, 31(2): 138-141.
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    ObjectiveTo investigate the role of NOD2 in the pathogenesis of experimental colitis in rats, and effects of oxymatrine injection on experimental colitis. MethodsThirtytwo SpragueDawley (SD) rats were randomly divided into four groups: normal control group, model group, mesalazine (SASP) group, and oxymatrine (OMT) group. Except normal control group, the rats in other three groups were generated colitis with trinitrobenzenesulfonic acid (TNBS). Rats in the model group, SASP group, and OMT group were treated for 15 days with distilled water, SASP, and OMT, respectively. Colon lesion score and histological score of experimental rats were detected. Expression of NOD2 protein in the colonic mucous of rats was tested by immunohistochemistry. Expression of TNFα in rats colon mucosa was checked by ELISA. ResultsCompared with the normal control group， the expression of NOD2 and TNFα in colonic mucosa of rats in the model group were significantly increased(P＜0.01). The NOD2 and TNFα expression in colonic mucosa of rats were significantly decreased in comparison to that of the model group(P＜0.05). ConclusionNOD2 protein plays an important role in the pathologic course of ulcerative colitis, while OMT attenuates ulcerative colitis through inhibiting expression of NOD2 and TNFα.
  • 药物研究
    Assessment of the Effects of Different Beta 2 Adrenoceptor Agonists on Cardiac Function in Isolated Rat Heart
    ZHU Lingyun
    2012, 31(2): 142-147.
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    ObjectiveTo evaluate the effects of beta 2 adrenergic agonists on cardiac function in isolated rat hearts.MethodsOn the basis of Langendorff perfussion into the isolated rat hearts, cardiac function in the influence of salbuterol sulfate，levalbuterol，metoprolol tartaric acid and formoterol fumarate on systolic pressure(LVSP)，both the maximum ascending and descending rate (+dp/dt,－dp/dt max) of left ventricle，and heart rate(HR) were monitored by using medlab recorder system. ResultsBeta 2 adrenergic agonists significantly increased LVSP，heart rate +dp/dt max and －dp/dt max of all groups compared with the control group(P＜0.05). Arrhythmias such as premature systole，tachycardia occurred and developed with the concentration increased. While，effects of levalbuterol and formoterol fumarate were significant weaker than those of salbutamol sulfate (P＜0.05). The selective beta 2 adrenergic blockers completely blocked effects of selective beta 2 adrenergic agonists; while selective beta 1 adrenergic partly blockers blocked effects of selective beta 2 adrenergic agonists. ConclusionThis study demonstrats that beta 2 adrenergic agonists possess certain degree of toxicity，levalbuterol is safer on heart that albuterol; Formoterol produces less impact on heart than buterol and terbutaline; No significant difference of security occurred between formoterol and levalbuterol.
  • 药物研究
    Antiinflammatory and Analgesic Effects of Rheumatism Ⅱ Mixture
    CHEN Li;WANG Qibin;LI Zhixiang;ZHAN Yan;ZHU Haitao;YE Lihong;WANG Gang
    2012, 31(2): 147-149.
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    ObjectiveTo study the antiinflammatory and analgesic effects of rheumatism II mixture. MethodsThe analgesic effect of rheumatism II mixture was studied by hot plate test and writhing response，and the antiinflammation of it was explored in the xyleneinduced ear swelling model，peritoneal exudate dye and egg whiteinduced paw edema in rats. The model rats were treated with 15.00，7. 50， 3.75 g•mL－1 of rheumatism II mixture as high， medium and lowdose treatment groups，respectively; with 50 mg•kg－1 hydrocortisone subcutaneously as the positive control group，and with an equal volume of 0.9% sodium chloride solution as the model control group.ResultsThe pain threshold was higher in all treatment groups and the positive control than that in the model control group in the hot plate test (P＜0.01)；the writhing of the high，mediumdose groups and the positive control group was significantly less than that of the model group in the acetic acid writhing test (P＜0.01)；the absorbance of each dose of treatment groups and the positive control was lower than that of the model group in the abdominal dye experiment (P＜0.01); the ear swelling and toe one of the high， mediumdose groups and the positive control were significantly less than those of the model group in the mouse swelling tests (P＜0.01). ConclusionRheumatismⅡ mixture has good antiinflammatory and analgesic effects by inhibiting the egg white mixtureinduced paw edema， xyleneinduced ear swelling and acetic acid induced writhing.
  • 药物研究
    Protective Effects of Ligustrazine on CisplatinInduced Oxidative Injury of Kidneys in Rats
    XUE Ying;LI Qixiong;LI Shuang
    2012, 31(2): 150-152.
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    ObjectiveTo investigate protective effects of ligustrazine (tetramethypyrazine, TMP) on cisplatininduced oxidative injury of kidneys in rats. MethodsTMP was administered at 50，100 mg•kg－1•d－1 body weight intraperitoneally (ip) for five consecutive days, 2 days before a single dose of cisplatin (DDP, 8 mg•kg－1，ip). The rats were sacrificed four hours after the last injection. Blood urea nitrogen(BUN) and serum creatinine (SCr) were tested, 24 hours urine were collected to detect urinary protein. The contents of malondialdehyde (MDA), glutathione (GSH) content, nitric oxide (NO), activities of superoxide dismutase (SOD), glutathioneStransferase (GST), the nitric oxide synthase (NOS) in the renal cortex were measured. ResultsCompared with DDP, TMP (50 and 100 mg•kg－1•d－1) dosedependently reduced 24 h urinary protein, BUN and SCr induced by cisplatin, by 41.45%,65.33%;18.12%,57.51%;14.39%,48.89%（P＜0.05, P＜0.01）, respectively. The 100 mg•kg－1•d－1 TMP significantly decreased levels of MDA, NO, NOS and GSH, SOD,GST induced by cisplatin in kidney by 36.73%, 45.39%, 22.86%( P＜0.05) , respectively; while, the content of GSH was increased by 1.33fold，1.66fold ,1.57fold （P＜0.05）, respectively, compared with that in the DDP group. ConclusionTMP could protect cisplatininduced oxidative nephrotoxicity.
  • 药物研究
    Antinociceptive Effects and Acute Toxicity of Xuanzhi Analgesic Tablet
    XU Lijun;WANG Kaifu;ZOU Xin;GONG Di;HUANG Guangying
    2012, 31(2): 153-155.
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    ObjectiveTo study the antinociceptive effects and acute toxicity of xuanzhi analgesic tablet. MethodsThe acetic acidinduced writhing response and the hotplate test in mice were taken to study analgesic effects and maximum tolerant dose were regarded as toxicological evaluation for the tablet. ResultsEach dose of the tablet showed significantly pain relieving effect in acetic acid induced mice model in a doserelated manner. Moreover, each dose started to attenuat pain induced by heat board at the same time as aspirin，but lasted longer; the MTD of which was 259.2 g•kg－1. ConclusionXuanzhi analgesic tablet possesses powerful analgesic effects，endures longer than aspirin and is safe.
  • 药物研究
    Effects of 2，3，5 4’tetrahydroxystilbene2OβDglucoside on Nitric Oxide Synthase and Gene Expression in Endothelial Cells of Human Umbilical Vein
    SHEN Jianfen;ZHANG Youzhi;XIAO Junhua;WANG Jialing
    2012, 31(2): 156-159.
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    ObjectiveTo investigate the effects of 2，3，5 4’tetrahydroxystilbene2OβDglucoside(THSG) on content of nitric oxide(NO) and activity of endothelial nitric oxide synthase (eNOS) and the mechanisms.MethodsThe human umbilical vein endothelial cells(HUVECs) were cultured and divided into four groups：the control，THSG（1，10，100 μmol•L－1）groups．Spectrophotometry endothelium assay was used to measure NO and activity of eNOS. RTPCR was used to analyze the eNOS mRNA expression and Western Blot was used to analyze eNOS protein expression level in HUVECs. ResultsTHSG significantly increased the production of NO，activated NOS and upregulated expression of eNOS mRNA and protein in a concentrationdependent manner compared with the control group. ConclusionTHSG raises expression of eNOS mRNA and protein，which may result in activating NO production and relaxing blood vessels.
  • 药物研究
    Effects of Propofol on Studying and Memory Dysfunction and Cortex TrkB/Akt Pathway in Focal Cerebral Ischemia/reperfusion Injury of Rats
    ZHANG Junfeng;ZHOU Wu;WANG Tao
    2012, 31(2): 159-161.
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    ObjectiveTo investigate effects and mechanism of propofol on the studying and memory dysfunction after ischemia/reperfusion in rats. MethodsPropofol or saline placebo was administered intraperitoneally prior to reperfusion and effects of which on TrkB/Akt pathway in nerve growth factor receptor were studied by Western Blot and immunohistochemistry in the model of memory and learning dysfunction caused by ischemia/reperfusion(I/R). ResultsThe latent period of I/R group was obviously shorter than that of the control group，(121.98±15.56) vs. (210.78±26.71) s; the number of cumulated stimulation of I/R group was obviously more than that of the control group，(119.36±13.15) vs. (42.78±5.22). The severe neuronlogic deficits，studying and memory function induced by ischemia/reperfusion were significantly improved after treated with propofol. TrkB expression and Akt activity were significantly increased by propofol. ConclusionPropofol ameliorates the studying and memory function, which may be associated with TrkB/Akt pathway activation.
    • 药物制剂与药品质量控制
  • 药物制剂与药品质量控制
    Evaluation of Standard Value and Measurement Uncertainty for Determination of Panax Notoginseng Total Ash and Acid-insoluble Ash
    LIU Shucong;WANG Xiaoying;ZHANG Li;MA Lin;LU Yang
    2012, 31(2): 195-198.
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    ObjectiveTo establish an evaluation of standard value and measurement uncertainty of total ash and acid insoluble ash from Panax notoginseng. MethodsTo establish a mathematical model for calculating standard value and measurement uncertainty of panax ash and acid insoluble ash by analyzing various factors affecting measurement. ResultsThe standard value and expanded uncertainty of panax ash and acid insoluble ash were（3.83±0.39）% and （0.21 ±0.04）%. ConclusionThe method created show traceability and can be used to assign standard value for Chinese herbal medicine.
  • 药物制剂与药品质量控制
    Determination of Impurities in the Oligonucleotide by Solid Phase Extraction Ion chromatography
    MA Jinping;ZHONG Jie;LU Dandan;ZHANG Jinzhong;SHI Mingzhu;GAO Chan;ZHU Deling
    2012, 31(2): 202-205.
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    ObjectiveAn solidphaseextraction ion chromatography was developed for determining three residual impurities such as acetic acid (MCAA)， dichloroacetic acid (DCAA) and chloride ion (Cl－) in crude oligonucleotide simultaneously. MethodsThe final optimized conditions for testing were listed as follows by using Ionpac AS 23 (4 mm×250 mm) analytical column，Ionpac AG23 (4 mm×50 mm) protective column, mobile phase as 0.8 mmol•L－1 NaHCO3/4.5 mmol•L－1 Na2CO3 and suppressed conductivity detector. ResultsThe standard curve equation of MCAA，DCAA and Cl－ were Y= 5.230 7X+0.014 (r=0.999 7)，Y=19.762X－0.007 2 (r=0.999 9) and Y=507.4X－0.746 2 (r=0.999 8), respectively; MCAA，DCAA and Cl－ showed a good linearity in 0.002－0.400 μg•mL－1， 0.000 624－0.156 μg•mL－1， 0.002 4－1.5 mg•mL－1 and detection limits were 0.02，0.312 ng•mL－1 and 0.01 μg • mL－1 ，respectively. ConclusionThe method is simple，fast，accuracy and can be used for impurities control of crude oligonucleotide.
  • 药物制剂与药品质量控制
    Contents of Total Amino Acids in Dendrobium crystallinum Tchb. F. with Pre-column Derivatization Method
    LI Guang;LI Xuelan;CHEN Xi;
    2012, 31(2): 206-208.
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    ObjectiveTo analyze the contents of amino acids in Dendrobium crystallinum Tchb. F. by using precolumn derivatization method. MethodsThe amino acids in Dendrobium devonianum were pretreated with 2，4dinitrofluorobenzene and their derivatives were analyzed on Waters 600 HPLC using Symmetry C18 column. Gradient eluent consisted of sodium acetate solution (pH=6.4) with 1% N，Ndimethylformamide， acetonitrile and water (1：1) was taken. The detection wavelength was 360 nm and column temperature was 35 ℃ and loading volume was 20 μL. ResultsThe calibration curves for amino acids showed good linearity(r=0. 999 5－1.000 0，n=5). The mean recoveries of amino acids were 99.34%－101.93% with RSD as 0.54%－1.91%(n=6). And the average content of amino acids was 3.79%. ConclusionThe method is quick，simple with minimum loss of amino acids, which provides a theoretical basis for the Dendrobium crystallinum development.
  • 药物制剂与药品质量控制
    Content Determination of Vitexicarpin in Different Organs of Vitex trifolia L. var. simplicifolia Cham
    SUN Weiyang;LI Jianfang
    2012, 31(2): 206-208.
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    ObjectiveTo provide scientific evidence about chosing useful parts and cultivation through studying vitexicarpin in different organs of Vitex trifolia L. var. simplicifolia Cham.MethodsHighperformance liquid chromatography (HPLC) was used for determination of vitexicarpin in different organs of Vitex trifolia L. var. simplicifolia Cham. ResultsThe results showed that vitexicarpin presented the highest amount in fruit(0.030 8%) and followed by that of leaf(0.012 0%), while vitexicarpin in branch and root couldn’t be detected by this protocol due to its low concentration after natural airing. ConclusionIt’s rational to use the fruit of Vitex trifolia for medication and further exploit application of the leaf.
  • 药物制剂与药品质量控制
    Determination of Astragaloside Ⅳ in Fuzheng Qiangjin Tablets by HPLCELSD
    XIE Wei;FANG Jianguo;DUGuang;WANG Wenqing;SHI Chunyang;WAN Jin
    2012, 31(2): 208-210.
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    ObjectiveTo establish a HPLC evaporative light scattering detector(ELSD) method for determination of astragaloside Ⅳ in fuzheng qiangjin tablets. MethodsThe astragaloside Ⅳ in the tablet was determined by HPLCELSD. HPLC system consisting of an Licrosorb C18 column (4.6 mm× 150 mm, 5 μm) and a mixture of acetonitrile–water (34：66) as mobile phase was adopted and flow rate was 0.8 mL•min－1. The flow rate of carrier gas was 1.6 L•min－1. The aerosolizer’s temperature was 49 ℃ and the gain was 4. ResultsA good linear relationship was found between the astragaloside Ⅳ kept within a range of 0.052－1.034 mg• mL－1. The average rate of recoveries was 102.2%（RSD=1.00%, n＝3）,99.1%（RSD=1.36%, n＝3）,102.4%（RSD=1.49%, n＝3）, respectively. ConclusionThis method possesses merits of good accuracy and reproducibility, which can be used for determination of astragaloside Ⅳ in fuzheng qiangjin tablets.
  • 药物制剂与药品质量控制
    Content Determination of Total Flavonoid in the Extracts of Ampelopsis megalophylla
    HE Xiaoyu;YANG Ruojing;ZHU Zhikai;JIANG Can;ZHANG Xiuqiao;LIU Yanwen
    2012, 31(2): 211-212.
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    ObjectiveTo determine and compare total flavonoid in different extracts of Ampelopsis megalophylla.MethodsThe extracts were produced by heating reflux with ethanol and percolation method, respectively. Ampelopsin was used as reference substance, and the content was detected at 311 nm. ResultsThe average recovery rate was 99.36%, RSD was 1.59%.The content of total flavonoid in the extracts by refluxation was 640.74 mg•g－1, which was 672.38 mg•g－1 by percolation. ConclusionThis method is simple and reliable, which is useful for content determination of total flavonoid in the extracts of Ampelopsis megalophylla. The extracts by percolation is purer than those isolated through refluxation.
  • 药物制剂与药品质量控制
    Preparation and Characterization of Rotenone Loaded Nanogel
    MA Li;ZHANG Zhiwen;JIANG Shijun;XUE Daquan
    2012, 31(2): 213-216.
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    ObjectiveTo prepare the rotenone loaded nanogel (RNG) with the PLGAPEGPLGA triblock copolymer. MethodsRNG was prepared by a film dispersion method. The size was measured by dynamic light scattering，and physical state of rotenone in RNG was analyzed by DSC and Xray diffraction. Moreover，in vitro release profiles of RNG were evaluated. ResultsRNG exhibitted nanometric particles with a mean diameter of 120 nm and narrow distribution (PDI=0.190). The DSC and XRD measurement indicated that rotenone existed as amorphous state in nanogel. Furthermore，in vitro release profiles of RNG supposed to be related with drug content in RNG and Tween 80 concentration in the release media. ConclusionRotenone loaded nanogel is successfully developed and conspicuously improves the aqueous solubility and in vitro release profiles of the poorly watersoluble drug.
  • 药物制剂与药品质量控制
    Formulation Optimization of Zuning Granules by Central Composite DesignResponse Surface Methodology
    GUO Junhao;DU Guang
    2012, 31(2): 216-219.
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    ObjectiveTo optimize the formulation of zuning granules by central composite designresponse surface methodology. MethodsThe content of extraction powder in preparation and amount of syndets added were studied on the theory of central composite design with moisture absorption，molding rate and solubility as evaluation indexes．The data were simulated using multilinear equation and secondorder equation polynomial equation．The possible optimal formulation was predicted by response surface method.ResultsRegression coefficients of secondorder quadratic models were superior to those of the firstorder model and with higher reliability. The optimized conditions was obtained with the ratio (X1) of the powdered extracts to excipients as 62.50%, the dosage (X2) of wetting agent added as 6.30%.ConclusionThe established model based on the theory of central composite design is suitable for preparation optimization of zuning granules.
  • 药物制剂与药品质量控制
    Content Determination of Some Components in Shengu Granule
    WANG Wenqing;LI Wei;LIN Meng;ZHANG Fei;HOU Xiaolong;WAN Jin;FANG Jianguo
    2012, 31(2): 219-222.
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    ObjectiveTo determine contents of some components in shengu granule and for providing the theory basis of quality evaluation. MethodsThe contents of calcium acetate, polysaccharide, amino acids, heavy metal, arsenic (As), plumbum(Pb) and phosphorus(P) in shengu granule were detected by using titration,phenol hydratesulfuric acid method, amino acids automatic analysis, colorimetry, gucai method, FAAS and andmolybdenumblue spectrophotometry, respectively. ResultsThere were 48.17% calcium acetate, 2.14% polysaccharide, 3.32% amino acids and 2.4×10－6 P and less than 1×10－6 heavy metal, 2×10－6 As and 0.5×10－6 Pb in the granule. ConclusionThe shengu granule contains high amount of nutrients, low amount of harmful components and is in good quality.
    • 用药指南
  • 用药指南
    Curative Effect of Insulin Pump in Treatment of Diabetic Ketoacidosis: A MetaAnalysis
    MEI Shasha;SONG Enfeng
    2012, 31(2): 248-252.
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    ObjectiveTo evaluate the curative effect of insulin pump in treatment of diabetic ketoacidosis(DKA). MethodsApplication of insulin pump in treatment of DKA was retrieved and collected electronically, and metaanalyses were conducted for randomized controlled trials (RCTs). ResultsNineteen RCTs were included.Compared with a regular continuous insulin injection, continuous subcutaneous insulin infusion(CSII) for treating DKA had more advantages. Fifteen studies indicated that the duration required for standardizing blood glucose in CSII was lower. Seven studies demonstrated that less average insulin dosage in CSII was needed. It took shorter time to eliminate ketonuria in CSII from fifteen RCTs. Insulin induced hypoglycemia in CSII was obviously decreased from twelve studies. And blood ketone was corrected quicker from four RCTs in CSII. ConclusionInsulin pump simulates physiological insulin secretion is effective and rapidly in correcting ketoacidosis. It is a safe, reliable, convenient and effective method for treating DKA, which enables a better quality of life.
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