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    药物研究
  • 药物研究
    Characterization of Scutellarin Phospholipid Complex
    LIU Hong;TANG Xiaoqiao;WANG Ying;YANG Xiangliang
    2010, 29(11): 1395-1397.
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    ABSTRACTObjectiveTo characterise the scutellarin phospholipid complex. MethodsInfrared spectroscopy (IR), differential scanning calorimetry (DSC) and laser particle size analyzer were use to detect the character of scutellarin phospholipid complex. ResultsDSC showed that phase transition temperature of the complex was slightly lower than those of scutellarin and phospholipid without significant change of range. When it was dispersed in water, the comlex appeared as a colloid at low temperature, while as a suspension at high temperature. The spherical particles were seen in diluted solution under microscope. Its average particle size was 831.7 nm. ConclusionThe combination between scutellarin and phospholipid is a infirm interaction.
  • 药物研究
    Effects of the Essential Oil from Angelica sinensis (Oliv.) Diels on Percutaneous Permeability of Nimodipine
    WANG Qingwei;ZHANG Jing;LIU Xueying;LI Xiaoye;XU Yuan;QI Zhihua
    2010, 29(11): 1397-1400.
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    ABSTRACTObjectiveTo study effects of the essential oil from Angelica sinensis (Oliv.)Diels on percutaneous permeability of nimodipine in rabbit skin in vitro. MethodsRabbit skin was used as permeation barrier and nimodipine was selected as a model drug. Franz diffusion cell was used to investigate the cumulative permeation amount of nimodipine which was determined by HPLC. The essential oil from Angelica sinensis (Oliv.) Diels at different concentrations were used as permeation enhancer and 30% alcoholsaline solution was used as reception solution. ResultsEssential oil at various levels from Angelica Sinensis enhanced the percutaneous absorption of nimodipine in vitro (all P＜0.05), 1% of which showed significant penetration enhancing effect and the permeability coefficients of nimodipine was 36.63 μg•（cm2）-1•h1, which was 3.25 times as much as that in normal saline. ConclusionThe essential oil from Angelica sinensis (Oliv.) Diels. could be used as the penetration enhancers in the transdermal preparation of nimodipine.
  • 药物研究
    Preparation of Paclitaxel Magnetic Nanoparticle Liposome and Anti-tumor Activity
    XIAO Chao;WU Xinrong
    2010, 29(11): 1401-1404.
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    ABSTRACTObjectiveTo prepare the paclitaxel magnetic nanoparticle liposome, and investigate its antitumor activity in vitro. MethodsIt was prepared by using membrane ultrasound disintegration method,which was optimized by orthogonal design, and the in vitro antitumor activity was investigated by MTT method. ResultsThe best preparation and formulation were as follows：lecithin：cholesterol=4：1, paclitaxel：(lecithin+cholesterol)=1：30, ultrasounding for 40 min, magneticpowder：paclitaxel=1：1, tween80：paclitaxel=4：1, polyethylene1 000：paclitaxel =8：1. The average encapsulation efficiency of the optimized liposome was 85.8%，the average particle size was 150 nm. Its antitumor activity on three types of cancer cells was good and with sustained release property. ConclusionThe paclitaxel magnetic nanoparticle liposome prepared by thin film ultrasonic method has a high encapsulation efficiency, evenly distribution, and potent antitumor activity，which meets the requirements for targeting preparation.
  • 药物研究
    Protective Effect of Ltetrahydropalmatine on Focal Cerebral Ischemia Reperfusion Injury in Rats
    CHEN Peng;MI Man;LI Bin
    2010, 29(11): 1405-1408.
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    ABSTRACTObjectiveTo study the protective effects of different doses of Ltetrahydropalmatine(LTHP) on focal cerebral ischemia reperfusion injury in rats and its mechanism. MethodsSixty healthy SD rats were randomly divided into six groups:①sham operation, ②ischemia reperfusion model(IR), ③nimodipine group(I-R+Nim10 mg•kg-1), ④large dose of Ltetrahydropalmatine group (IR+LTHP50 mg•kg-1), ⑤ middle dose (IR+LTHP25 mg•kg-1),⑥ low dose (IR+LTHP 12.5 mg•kg-1). We used the Modifiied Longa method to make the model of focal cerebral ischemia reperfusion injury in rats, except the sham operation ones. Infarct size,hydropic volume of brain,neurological deficit scores, superoxide dismutase(SOD) activity,malondialdehyde(MDA) content in serum of rats were measured. ResultsThe results indicated that the ischemia reperfusion injury resulted in high neurological deficit scores, large brain infact size and hydropic volume, decreased activity of SOD and increased content of MDA. It was showed that different doses of LTHP improved all signs mentioned above. Compared with the model control, LTHP I and II groups had significantly statistical meaning (P＜0.01), LTHP III was P＜0.05. ConclusionLTHP has the protective effects on rat brain damage induced by focal cerebral ischemia reperfusion, the mechanism of which might be associated with attenuating the injury by free radicals.
  • 药物研究
    Uptake and Transport of Eriocalyxin B in Caco2 Cell Monolayers
    DONG Ruihua;LIANG Yuguang;SHAN Tingting;GAO Hongzhi;LIU Zeyuan
    2010, 29(11): 1408-1411.
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    ABSTRACTObjectiveTo study the characteristics of uptake and transport of eriocalyxin B（ERB）through Caco2 cells. MethodsA human intestinal epithelial cell model Caco2 cell was applied to study the characteristics of uptake and transport of ERB, in which time, concentration and temperature were investigated. The determination of ERB was performed by HPLC and apparent permeability coefficients (Papp) was calculated. ResultsIt was showed that the apical to basolateral(AB)permeability(Papp) of ERB positively correlated to time and temperature before 45 minutes, and positively correlated to concentration within the effective concentration range. ConclusionERB is mainly absorbed and transported through membrane of intestinal epithelial cells by passive diffusion.
  • 药物研究
    Preparation and in vitro Evaluation of PaclitaxelLoaded Poly(lactidecoglycolide) Nanoparticles
    JI Shunli;LI Bo;LI Zhen;WANG Chengrun;JIN Yi;GE Yanru
    2010, 29(11): 1411-1415.
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    ABSTRACTObjectiveTo investigate the preparation and in vitro evaluation of paclitaxel nanoparticles with a poly(lactidecoglycolide) carrier. MethodsTAXPGLANPs were prepared by the modified solvent diffusion method. The different type of emulsifier and process parameters involved were investigated with respect to the nanopartide mean size. The properties of nanoparticles were primarily studied by a dynamic laser particle size analyzer, transmission electron microscopy and Xray powder diffraction (XRD). In addition, in vitro release characteristics of freezedried powder was also investigated. ResultsThe preparation process was optimized as follows: the volume ratio of water to organic phase was 20：10, both the surfactant and polymer concentration were 1%, the homogenizer speed was at 16 000 r•min-1, the drug concentration was 0.1%. The optimized result showed that TAXPLGANPs were spherical with an average diameter of 99.0 nm, Zeta potential of 58.3 mV, drugloading content of 7.10%, encapsulation efficiency of 56.77%. The release behavior of drug in vitro showed a sustainedrelease, with an initial burst effect, subsequently a slower rate stage. The entrapment of TAX in nanoparticles was ensured by DSC and XRD analysis. ConclusionPLGANPs might be used as a new carrier for paclitaxel.
  • 药物研究
    Effects of the Extracts and Different Polar Parts of Kansui on Tumor Cells Proliferation
    CAO Yan;ZHOU Yunyun;SONG Chengwu;HONG Yi;YU Shanggong;FANG Nianbo
    2010, 29(11): 1416-1418.
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    ABSTRACTObjectiveTo investigate the growthmodulating effects of the extracts and different polar parts of Kansui on the proliferation of MCF7, A549, HepG2 cells. MethodsThe extracts of Kansui, acetate extract, cyclohexane extract and water extract from Kansui were cocultured with MCF7, A549, and HepG2 cell lines, and the cell proliferation was evaluated by MTT colorimetric assay. ResultsThe ethanol extract of Kansui obviously reduced MCF7 proliferation, with IC50 values as 202 μg•mL1;Acetate extract and cyclohexane extract from Kansui suppressed MCF7 as well, with IC50 as 552 μg•mL-1 and 378 μg•mL-1, respectively. They stimulated the growth of A549 and HepG2 cells at a high concentration. The water soluble parts from Kansui stimulated A549 and HepG2 cells proliferation at high levels. ConclusionThe anticancer components in Kansui could be exist in acetate extracts and cyclohexane extracts, while the cancerstimulating compotents be in the water extracts from Kansui.
    • 论文
  • 论文
    Effects of Phycocyanin on Expressions of IGF1 and IGF1R after Cerebral Ischemia reperfusion in Rats
    SUN Feng;DING Xiaojie;WANG Chao
    2010, 29(11): 1418-1421.
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    ABSTRACTObjectiveTo study neurological deficit scores and expressions of insulinlike growth factor1 (IGF1) and IGF1 receptor (IGF1R) after focal cerebral ischemiareperfusion (I/R) in rats, and explore the interference effects of phycocyanin on cerebral I/R injury. MethodsModels of middle cerebral artery occlusionreperfusion (MCAO/R) were established by the intraluminal filament technique in 64 healthy adult male Wistar rats, which were blindly randomized into 3 groups: sham operation group (n=4), control group (n=30) and treatment group (n=30). We set up 6 observation phases(6，12 h，1，3，7，14 d)after focal cerebral I/R in the last groups. Phycocyanin was administered to rats in the treatment group and normal saline at the same dose was given to rats in the control group; the sham operation group was untreated. Neurological deficit scores were measured using the Bederson test and expressions of IGF1 and IGF1R were determined by immunohistochemical staining, to evaluate the effects of phycocyanin on above indexes. ResultsNeurological deficits of different degrees occurred to rats in the control group and expressions of IGF1 and IGF1R in the ischemic brain tissues were evidently higher than those in the sham operation group, especially in the cortex and striatum. The neurological deficit score of rats in the treatment group was evidently lower than the control group after 2448 h I/R and symptoms of the former were obviously alleviated. The trend of changes of IGF1 and IGF1R in the cortex and striatum was similar to those in the model control group, but evidently higher than those in the control group at the same time point. ConclusionPhycocyanin may exert its neuroprotective effect through upregulating the expressions of IGF1 and IGF1R after cerebral I/R
    • 药物研究
  • 药物研究
    Effect of Telmisartan on Renal Interstitial Fibrosis in Rats Caused by Unilateral Urethral Obstruction
    ZHANG Jue;SONG Enfeng;DING Qingyu
    2010, 29(11): 1422-1425.
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    ABSTRACTObjectiveTo investigate the effects of Telmisartan on renal fibrosis in rats caused by unilateral urethral obstruction (UUO) and its mechanism. MethodsAdult male SD rats (n=24) were randomly divided into three groups: Sham  operated group for control(Sham), UUO group, Telmisartan group(treated with Telmisartan at 10 mg•kg1•d1 2 d before operation). Rats were sacrificed 2 weeks after operation. BUN and Scr in sura were measured. The renal interstitial fibrosis was observed under light microscopy with Masson and HE staining, and the expression of αSMA and FN were detected by immunohistochemistry. ResultsThe levels of BUN,and Scr, and the degree of renal interstitial fibrosis in UUO group and Telmisartan group were significantly higher than those in Sham group. The expression of aSMA and FN in UUO group significantly increased in comparison to those in the sham operation group（P＜0.01）. Compared with the UUO group, the Telmisartan group had significantly decreased expression of aSMA and FN (P＜0.05). ConclusionThe renal αSMA and FN are highly expressed in the process of renal interstitial fibrosis. Telmisartan inhibits the renal interstitial fibrosis lesion, decreases BUN and Scr levels and down  regulates the expression of αSMA and FN in UUO rats.
  • 药物研究
    Antiinflammatory and Immune Modulation Activities from Active Fraction of Periplocaforrestii Schltr
    WU Rongrong;ZHANG Xiaorui;SUN Lei;QI Chunhui
    2010, 29(11): 1425-1428.
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    ABSTRACTObjectiveTo find out the fraction with antiinflammatory and immune modulatory activities form Periploca forrestii Schltr (HGT), and evaluate its action. MethodsCroton oilinduced ear edema was used to assess the antiacute inflammation effect of HGT5A. 2,4Dinitrochlorobenzene(DNCB)induced delayed hypersensitivity（DTH） response was employed to observe the effect on cellular immune response. ResultsHTG5A significantly inhibited croton oilinduced ear edema at 100－500 mg•kg-1 (ig) and also surpressed DNCBinduced cellular immune response at 50－100 mg•kg-1 (ig). The splenocyte proliferation was decreased after incubation with HGT5A. ConclusionHGT5A has strong antiinflammatory and immune regulatory activities.
  • 药物研究
    Study on the HSSPME for the Cortex Magnoliae officinalis of Hubei Enshi
    WANG Huaqing;ZHANG Linbi;YANG Min;LEI Mi;LIU Yimei
    2010, 29(11): 1428-1430.
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    ABSTRACTObjectiveTo optimize the headspacesolid phase microextraction (HSSPME) for the Cortex Magnoliae officinalis of Hubei Enshi.MethodsThe optimizations for specimen’s quantity, extraction time, extraction temperature, resolutional time and headextraction were carried out. ResultsThe best way was that taking 1.2 g specimen, extracting for 90 min, at 90 ℃, resolving for 3 min and using PDMS/DVB as fiber. ConclusionThe final results provide the best optimizations of HSSPME for the Cortex Magnoliae Officinalis of Hubei Enshi.
  • 药物研究
    The Effects of Different Preparation Methods on the Properties of Nimodipine Liposomes in vitro
    XIA Jing;TAN Ye
    2010, 29(11): 1430-1432.
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    ABSTRACTObjectiveTo study the effects of various liposome preparation methods of Nimodipine on the properties of the liposomes in vitro and choose the best method. MethodsDifferent preparation methods，including film dispersing，reverse phase evaporation，high pressure homogenisation were used to prepare the nimodipine liposome. Transmission electron was employed to observe the morphology and entrapment efficiency was studied .ResultsThe liposomes were globular versicles.the order of entrapment efficiency was: film dispersing ＞ reverse phase evaporation ＞ high pressure homogenisation.The order of the mean particle size was: film dispersing ＞ reverse phase evaporation ＞ high pressure homogenization.ConclusionThe quality of liposomes in vitro was significantly relevant to liposome preparation methods.
    • 药物与临床
  • 药物与临床
    20 Patients with Intractable Pain Treated by Botulinum Toxin A Along Nerve
    FAN Ronglan;LIU Shuangyu;LI Yaling;LI Ruihong
    2010, 29(11): 1452-1453.
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    ABSTRACTObjectiveTo study the effect of botulinum toxin A on refractory postherpetic neuralgia. Methods40 patients with herpes zoster neuralgia, excluded other pain causes, were randomly divided into treatment group and control group, 20 cases in each group. All 40 patients were treated with antivirus, nutrition, symptomatic. The patients in the treatment group was treated with botox A along nerve, and the injection volume was 0.1 mL containing 25,000 units every point. ResultsThere’s no difference (P＞0.05) between treatment group and control group after being treated 1 day for the pain points, which was 6.9±0.85 and 7.8±0.7, respectively. After treated for 3 days, 7 days, 14 days and 30 days, the pain scores of treatment group decreased significantly, and the differences between treatment group and control was significant as well(P＜0.05 for all). ConclusionThe injection of botox A along nerve can cure herpes zoster neuralgia, and improve the energy, appetite and sleep of the patients efficiently.
    • 药物制剂
  • 药物制剂
    Optimizing the Extraction Condition of Semibionic Extraction for Salvia MiltiorrhizaBge.of Fangling by Uniform Design
    YE Fang;YANG Guangyi;HUANG Liangyong;HAO Xincai;WANG Gang;YANG Biao
    2010, 29(11): 1469-1472.
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    ABSTRACTObjectiveTo optimize the extraction condition of semibionic extraction for watersoluble parts from Salvia miltiorrhiza Bge. MethodsThe best extraction condition of the semibionic extraction for Salvia miltiorrhiza Bge. by uniform design was optimized regarding contents of total phenolic acid and salvianolic acidB and dried extract obtained. ResultsThe pH values of water were in order as 2.0，7.5，8.0，which extracting for 1.0，0.5，0.5 h. ConclusionThe optimized extraction condition is scientific and rational.
  • 药物制剂
    Preparation of Medicinal Charcoal Clysma
    WANG Zhen;DING Yufeng;HUANG Xutong;FANG Shuxian;DU Guang
    2010, 29(11): 1472-1474.
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    ABSTRACTObjectiveTo determine the best technic for medicinal charcoal clysma preparation. MethodsThe procedure was optimized by orthoral test, L9(34). ResultsThe best preparation for medicinal charcoal clysma was A2B2, using 0.5% CMCNa and 2.0% HPMC as thickener. ConclusionThe optimized technic is credible, and can be used to promot the stability of medicinal charcoal clysma.
    • 药品质量控制
  • 药品质量控制
    Analysis of Proanthocyanidins in Grape Seeds by RRLCMSMS
    SU Yeping;DU Ping;JIN Jianxiu;XU Qiaoxia;LIU Honghai
    2010, 29(11): 1479-1481.
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    ABSTRACTObjectiveTo assay the chemical compositions of proanthocyanidins in grape seeds by RRLCMSMS. MethodsThe grape seeds extraction was purified by HPD400A macroporous adsorption resin, separated by RRLCMSMS and chemical compositions of which were analyzed by information of MSMS. ResultsThree monomers, nine dimmers, five trimers and one tetramer of proanthocyanidins in grape seeds were preliminarily isolated and identified. ConclusionThe major compositions of proanthocyanidins in grape seeds could be well separated and analyzed by RRLCMSMS.
  • 药品质量控制
    Content Determination of Ursolic Acid in Dipsacus asper Wall
    JIN Xiaoping;LIU Jingyou;MA Dan;LONG Qinge;ZHAO Yake
    2010, 29(11): 1481-1483.
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    ABSTRACTObjectiveTo establish a method for determination of ursolic acid as a triterpene in Dipsacus asper Wall., and which is used as the quality control of Dipsacus asper Wall.MethodsThe Reliasil C18 was used in the HPLC method and mobile phase was composed as methanol- water - triethylamine(90：10：0.03, pH＝6.0 adjusted by acetic acid) with detection wavelength at 210 nm and with column temperature at 23 ℃. ResultsRetention time was 12.35 min, the linear ranges of ursolic acid was 23.8～714.0 μg•mL-1 and the average recovery of ursolic acid was 98.73 %, RSD=0.51%. ConclusionThis method is simple, susceptible, accurate, and suitable for the determination of ursolic acid in Dipsacus asper Wall.
  • 药品质量控制
    Content Determination of Ursolic Acid in Dipsacus asper Wall
    JIN Xiaoping;LIU Jingyou;MA Dan;LONG Qinge;ZHAO Yake
    2010, 29(11): 1481-1483.
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    ABSTRACTObjectiveTo establish a method for determination of ursolic acid as a triterpene in Dipsacus asper Wall., and which is used as the quality control of Dipsacus asper Wall.MethodsThe Reliasil C18 was used in the HPLC method and mobile phase was composed as methanol- water - triethylamine(90：10：0.03, pH＝6.0 adjusted by acetic acid) with detection wavelength at 210 nm and with column temperature at 23 ℃. ResultsRetention time was 12.35 min, the linear ranges of ursolic acid was 23.8～714.0 μg•mL-1 and the average recovery of ursolic acid was 98.73 %, RSD=0.51%. ConclusionThis method is simple, susceptible, accurate, and suitable for the determination of ursolic acid in Dipsacus asper Wall.
    • 用药指南
  • 用药指南
    Costminimization Analysis of Four Therapeutic Regimens for Abnormal Liver Function Tests Caused by Chemotherapeutics
    HUANG Yanjing;GUI Ling;YU Shiying
    2010, 29(11): 1503-1505.
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    ABSTRACTObjectiveTo evaluate the economic effects of four therapeutic regimens for treatment of abnormal liver function tests caused by chemotherapeutics. Methods134 patients with liver function tests abnormalities caused by chemotherapeutics were retrospectively investigated, and divided into four groups: group A (VitC+inosine), group B (VitC+inosine+ diammonium glycyrrhizinate), group C(VitC+ inosine+ reduced glutathione) and group D(VitC+ inosine+ magnesium isoglycyrrhizinate). Evaluation for these therapeutic regimens was performed with pharmacoeconomic costminimization analysis. ResultsThe total costs in group A, B, C and D were 566.90, 634.40, 771.90 and 986.90 yuan, respectively; the effective rates were 51.72%, 54.55%, 55.56% and 61.11%, respectively(P＞0.05). ConclusionThe group D shows the most effective among four groups, but no significant difference is found among them. The cost of group A is lowest, therefore Group A(VitC+ inosine) is the most economical therapeutic regimen for liver function tests abnormalities caused by chemotherapeutics.
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