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    药物研究
  • 药物研究
    Effects of Cyclopamine on Experimental Choroidal Neovascularization and Expression of Glil,HIF1α and VEGF
    HE Hua;LI Guigang;WANG Zhitao;HE Xiaoyang
    2010, 1(8): 980-985. https://doi.org/10.3870/yydb.2010.08.002
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    ObjectiveTo investigate the efficacy and mechanism of cyclopamine on experimental choroidal neovascularization （CNV）.MethodsA Brown Norway rat model of CNV was induced by laser photocoagulation （140 mW,75 μm,0.1 s）and rats were divided into blank control, PBS control and two cyclopamine treated groups （2.5 mg·mL1, 4.0 mg·mL1）.Except for the blank control, rats were intravitreally injected with 5 μL PBS, 2.5 mg·mL1and 4.0 mg·mL1 of cyclopamine every other day, respectively, since the first day till day13 of photocoagulation.On day 14, fluorescence fundus angiography and indocyanine green eyeground angiography were carried out.The eye samples were moved out to make choroidal flatmounts marked with FITC dextran and histologic analysis.The Sonic hedgehog （Shh） Gli signal cascade reaction and mRNA expression level of Glil, hypoxia inducible factor （HIF）1α and VEGF in HIF1αVEGF signal cascade reaction were further analyzed by realtime PCR. ResultsThe laserinduced rat CNV was significantly inhibited by cyclopamine（P＜0.05）, and which dosedependently decreased the mRNA expression of Glil, HIF1α and VEGF（P＜0.05）.ConclusionThe activation of Sonic hedgehog signaling pathway and HIF1αVEGF cascade are implicated in the development of experimental CNV.Cyclopamine as Sonic hedgehog signaling inhibitor could become a novel effective therapeutic medicine for blocking CNV formation.
  • 药物研究
    Inhibition on αGlucosidase by Rhubarb Polysaccharides
    ZHANG Haifeng;DONG Yalin;LIU Linna;ZHANG Yan
    2010, 1(8): 985-989. https://doi.org/10.3870/yydb.2010.08.003
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    ObjectiveTo extract polysaccharide from rhubarb, determine the content and study the αglucosidase inhibition effect on the enzymeinhibitor model and Caco2 cells model for preliminary exploring the potential value of rhubarb polysaccharides on treating diabetes. MethodsRhubarb polysaccharides were extracted by water isolation and alcohol precipitation, and the content was determined with phenolsulfuric acid colorimetry. The inhibitory activity of rhubarb polysaccharides on αglucosidase (EC 3.2.1.20) was measured, as well as αglucosidase expressed on Caco2 cell model by using oxidase colorimetric method. ResultsThe average concentration of rhubarb polysaccharides in rhubarb extracts was (23.08 ± 0.89)%, RSD= 3.87% (n= 3), and average recovery was 97.59%, RSD= 3.15% (n= 6). With maltose as the substrate in the enzymeinhibitor model, the inhibition rates of 125，250，500，1 000 μg·mL1 rhubarb polysaccharides were 33.09%, 24.43%, 33.14%, 31.73%, respectively. The inhibition rate of 250 μg·mL1 positive drug (acarbose ) was 80.45%. On the Caco2 cell model, the inhibition rates of 125，250，500，1 000 μg·mL1rhubarb polysaccharides were 23.43%,26.23%,28.24%,29.17%, and 250 μg·mL1positive drug (acarbose) reached 84.08%. ConclusionWater extraction and alcohol precipitation for Rhubarb polysaccharides isolating and phenolsulfuric acid colorimetric assay for determining content of polysaccharide are simple and feasible. Rhubarb polysaccharides has a certain degree of αglucosidase inhibitory activity.
  • 药物研究
    Effects of Kallikrein on Pulmonary Arterial Hypertension in Rats Induced by Chronic Hypoxic Hypercapnia
    YANG Hanwen;ZHUGE Yi;WANG Xiaotong;JINLu;LIU Yinhua;YUAN Hai
    2010, 1(8): 989-993. https://doi.org/10.3870/yydb.2010.08.004
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    ObjectiveTo investigate the effects of kallikrein on pulmonary arterial hypertension induced by chronic hypoxic hypercapnia in rats. MethodsThirty male SpragueDawley rats were randomly divided into three groups (10 in each)：the control (NC group)，hypoxic hypercapnic+normal saline (HH group)，hypoxic hypercapnia+Kallikrein group(HC group). The rats in both HH and HC groups were placed in the isobaric cabin where concentration of O2 was within 9 %～11 % and concentration of CO2 was maintained within 5 %～6 %(8 hours/day, 6days/week, total 4 weeks).At the rest time, they lived under the same condition as those in NC group.At the beginning of the fourth week, the rats in HC group were injected with Urinary Kallidinogenase (1.6×102PNA·kg1 ) and those in the HH group were injected with normal saline through its tail vein before put into the cabin, for total 6 days. By the end of the 4th week, The mPAP was measured by external jugular vein cannula, the expression of eNOS and eNOSmRNA were detected by immunohistochemistry and RTPCR, respectively. ResultsCompared with the NC group, mPAP, OD of eNOSmRNA, WA／TA(vessel wall area／total area) and PAMT(media thickness of pulmonary arterioles) in both HH and HC groups were significantly higher (P＜0.01). Compared with the NC group ,RV/(LV+S) in HH group was significantly higher (P＜0.01).The expression of eNOS on small arterial and bronchi and eNOSmRNA in pulmonary were lower（P＜0.01）.Compared with the HH group,the values of mPAP, RV/(LV+S), OD of eNOSmRNA, WA／TA and PAMT in the HC group were significantly lower (P＜0.01). The expression of eNOS in HC group rats’ pulmonary was higher. ConclusionKallikrein may reduce rats’ pulmonary arterial hypertension induced by chronic hypoxic hypercapnia in our experiment due to activating of eNOS and alleviating vascular remodeling.
  • 药物研究
    The Effect of Extraction of Smilax china L. on Transcription Level of Bcl2 Gene in Hut 78 Cells
    GAO Li;PENG Xiaoming;HUO Shixia;ZHAO Mo;TANG Xiaoqin;YAN Ming
    2010, 1(8): 993-995. https://doi.org/10.3870/yydb.2010.08.005
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    ObjectiveTo investigate the effect of Smilax china L. extraction on the transcription level of Bcl-2 gene in Hut-78 cells. MethodsThe light density of of each group by semiquantitative RTPCR was measured after being treated, to analyze the change of the transcription level of Bcl-2 gene by calculating Bcl2/GAPDH. ResultsThe extraction of Smilax china L inhibited the transcription level of Bcl2 gene,and showed the best at a concentration of 8 μg·mL-1. ConclusionThe extraction of Smilax china L could reduce the transcription level of Bcl-2 gene in Hut-78 cells.
  • 药物研究
    Antiinflammatory Effect of Keerkang Oral Liquid
    GUO Qiang;LI Mengquan;ZHAO Yujia;LI Houzhong;JIN Xiudong
    2010, 1(8): 996-997. https://doi.org/10.3870/yydb.2010.08.006
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    ABSTRACTObjectiveTo study the antiinflammatory action of keerkang oral liquid.Methods40 rats were randomly divided into 5 groups: blank control group （0.9% NaCl）, prednisone group（0.2 mg·kg1） and groups of the three kerkang oral liquid （6.68, 3.34 and 1.67 mL·kg1）.Swelling of rat’s toe caused by injecting egg albumin was observed; number of migrated leucocytes caused by injecting CMC was counted; and weight of granuloma caused by implanting tampon was measured.ResultsThe toe swelling was reduced by different dosages of keerkang oral liquid at different time.The number of migrated leucocytes was decreased significantly by high and middle dosages of it. keerkang oral liquid obviously lowered the weight of granuloma caused by tampon embeded.ConclusionKeerkang oral liquid has an antiinflammatory action.
  • 药物研究
    GABAA Receptor Partially Mediated Propofolinduced Spatial Learning and Memory Dysfunction in the Ventral Hippocampus of Rats
    FENG Zhihui;YUAN Liyong;SHAO Yongping;DAI Tijun
    2010, 1(8): 998-1001. https://doi.org/10.3870/yydb.2010.08.007
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    ObjectiveTo study the relationship between gammaaminobutyric acid （GABA）A receptors in the ventral hippocampus and the propofolinduced spatial learning and memory dysfunction of rats.Methods112 Sprague－Dawley rats were divided randomly into 14 groups（n＝8）.Y－maze was used,and the numbers of lightning shock were evaluated as space learning and memory performance.The model of space learning and memory dysfunction was established by i.p.injection of propofol in rats.After the pipes were inserted into ventral hippocampus of rats, different dosage of bicuculline,a specific GABAA receptor antagonist bicuculline（Bic）,was infused.The amendment of propofol induced learning and memory dysfunction by different doses of Bic（0.125，0.25，0.5 mmol·L1，1μL）was observed.ResultsThe space learning and memory dysfunction was observed after 20 mg·kg1 propofol was injected into rats’ abdominal cavity, compared with NS, fat milk and 5 mg·kg1,10 mg·kg1Pro, P＜0.01, which was improved parallelly with the increased dosage of bicuculline, 0.125，0.25，0.5 mmol·L1，1 μL（P＜0.05）.However,the performance of space learning and memory of rats received the maximum dosage of bicuculline（0.5 mmol·L1，1 μL）was lower than that of rats in solventia（fat milk and artificial cerebrospinal fluid） group, P＜0.05.ConclusionPropofol may partially induce spatial learning and memory dysfunction in the ventral hippocampus of rats through GABAA receptor.
  • 药物研究
    Evaluation of Sensitivity of Tumor Cell to Chemotherapeutic Drugs Using MTT Assay
    LU Yan
    2010, 1(8): 1002-1005. https://doi.org/10.3870/yydb.2010.08.008
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    ObjectiveTo study the vitro MTT assay for determining the drug sensitivity in human tumors. Methods18 samples of leukemias, 15 brain tumors, 8 gastric cancers, and 8 breast cancers were cultured in vitro for determinating inhibitory rate of chemotherapy drugs to cancer cells by MTT. ResultsThere was significant difference in drug sensitivity to different cancer cells in vitro. The average inhibition against cancer cells conformed to their clinical effects. ConclusionThe MTT assay is sensitive, specific, rapid, simple and with high probability of sucess, which is important for clinical use and individual medication.

  • 药物研究
    Compare the Hypoglycemia Effect of Two Kinds of Tinospora sagittata （Oliv.） Gagnep.
    WANG Yonghui;YANG Guangyi;RAN Peihong;YE Fang
    2010, 1(8): 1005-1007.
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    ObjectiveCompare the hypoglycemia effect of Tinospora capillipes Gagnep and Tinospora sagittata （Oliv.）Gagnep. MethodsThe hyperglycemia model in mice was induced by alloxan, and the mice were i.g. administered with the semibionic extracts of T.capillipes and T.sagittata. The metformin was fed as a positive control. The hypoglycemic effects of two extracts were compared by testing blood sugar levels before and after administration, and the percentage of decrease. ResultsBoth T.sagittata and T.capillipes showed significant hypoglycemic effect, while, highdose of T.sagittata decreased blood sugar more obviously than that of T.capillipes group. ConclusionThe hypoglycemia effects of T.sagittata are better than that of T.capillipes.
    • 消化内科药物专栏
  • 消化内科药物专栏
    Effect of Rabeprazole Combined with Rebamipide in the Treatment of 36 Patients of Active Gastric Ulcer
    SHANG Xinjie;WANG Changxiong;CHEN Guanglan;LI Hongguang;XU Weihong;YE Jietong
    2010, 1(8): 1010-1012. https://doi.org/10.3870/yydb.2010.08.011
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    ObjectiveTo evaluate therapeutic effects of rabeprazole combined with rebamipide on active peptic ulcer of Helicobacter pylori infection, including clinical symptoms，endoscopic mucosal inflammatory changes，and the ulcer recurrence rate in sixmonth and 1year follow up observation. MethodsA randomized controlled clinical study in parallel was carried out.72 cases of gastric ulcer with active Hp infection were randomly divided into treatment group and control group, 36 cases in each.In the treatment group: Hp eradication triple plan combined with rebamipide was given at the first week, 100 mg tid; at the following 2 to 8 week, rabeprazole 10 mg, qd, and rebamipide,100 mg tid.For the control group: Hp eradication triple plan was taken for the first week; and omeprazole 20 mg per day was administered in the following 2 to 8 weeks.At the end of 8 weeks, gastroscopy and pathological examination were performed, and the ulcer recurrence rate were observed in the followup sixmonth and 1year for all patients.ResultsAfter treatment,clinical symptoms like mucosal ulcer healing and inflammation were significantly improved under gastroscopy and endoscopy, compared with the control; and 1year follow up ulcer recurrence rate was lower than the control, the difference showed statistically significance.ConclusionRabeprazole combined with rebamipide could alleviate ulcer symptoms, subside inflammation, improve the quality of ulcer healing and reduce ulcer recurrence rate.
  • 消化内科药物专栏
    Curative Effect of Bifid Triple Viable Bacteria Capsules Combined with Trimebutine Maleate Tablets in Treating Diarrhea Irritable Bowel Syndrome
    TANG E
    2010, 1(8): 1013-1015. https://doi.org/10.3870/yydb.2010.08.012
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    ObjectiveTo investigate the curative effect of bifid triple viable bacteria capsules combined with trimebutine maleate tablets on the treatment of diarrhea irritable bowel syndrome.Methods75 patients with diarrhea irritable bowel syndrome were divided into two groups: 38 patients of treatment group treated with trimebutine maleate tablets,0.2 g,tid,po and bifid triple viable bacteria tablets,40 mg,bid,po, 37 patients of control group treated with trimebutine maleate tablets in the same therapeutic way.The clinical symptom of two groups was compared before and after treatment,respectively,and clinical efficacy was evaluated after treatment.ResultsThe symptoms of treatment group were significantly improved than the control group.The total effective rate of it （92.11%） was higher than that of the control （81.08%）, P＜0.05.ConclusionThe treatment of bifid triple viable bacteria tablets combined with trimebutine maleate tablets can provide a satisfactory effect for patients with diarrhea irritable bowel syndrome.
    • 药物与临床
  • 药物与临床
    Comparison of Chemotherapy for Elderly Advanced Gastric Cancer by OLF and ELF
    2010, 1(8): 1024-1026. https://doi.org/10.3870/yydb.2010.08.017
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    ObjectiveTo compare the efficacy and toxicity of different chemotherapies of OLF and ELF for elderly advanced gastric cancer.Methods48 patients, diagnozed as gastric cancer via histopathological examination,were randomly divided into two groups, treatment group and control group,with 24 cases in each group, treated for at least two cycles, 21 days per cycle.ResultsIn the treatment group,CR 2, PR 10, NC 5 and PD 7.In the control group, CR 1, PR 9, NC 6 and PD 8, no significant difference was found between two groups（P＞0.05）.The main toxicities were myelosuppression,gastrointestinal reactions and reversible handfoot skin reaction.ConclusionThe shortterm chemotherapeutic efficacy of OLF is satisfied for elderly advanced gastric cancer and the toxicity can be tolerated.It is an effective treatment for the elderly patients with advanced gastric cancer.
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