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    药物研究
  • 药物研究
    Comparative Study of Two Kinds of Decolorant on Two Kinds of Animal Vitiligo Models
    ZHANG Lanlan;YAN Ming;LIU Xiaodong;HUO Shixia
    2009, 28(6): 690-692. https://doi.org/10.3870/yydb.2009.06.002
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    (1. ABSTRACTObjectiveTo compare the effects of hydroquinone and hydrogen dioxide on vitiligo in guinea pig and C57BL mice by observing the melanocyte, levels of melanin granule contained,and tyrosinase and immunoglobulin G,M,A in serum. MethodsThe vitiligo animal model was established with decolorant. DOPA and iron dyeing were used to detect quantity of melanocyte,contents of melanin granule，immunohistochemistry was applied in analyzing the content of tyrosinase,and the automatic biochemistry analyzer was used to determine serum IgG, IgM, IgA. ResultsAfter treated with both hydroquinone and hydrogen, the melanocyte, contents of melanin granule, tyrosinase and immunoglobulin G,A,M in serum of both guinea pig and C57BL mice were lower than those in the normal animals, except for the content of immunoglobulin M in C57BL mice. ConclusionBoth guinea pig and C57BL mice are elegible for establishing vitiligo animal model by being decolorized with hydroquinone and hydrogen dioxide.
  • 药物研究
    Determination of Rhodamine 123 in Rat Plasma by HPLCFluorescent
    HUANG Beibei;LI Guofeng;LI Qing;SUN Yabin;LIU Sijia
    2009, 28(6): 693-695. https://doi.org/10.3870/yydb.2009.06.003
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    ABSTRACTObjective To establish a HPLCfluorescent detector method for determining the concentration of rhodamine 123 in plasma. Methods The chromatographic separation was carried out on BTD4703 Packing C18 column with mobile phase consisted of acetonirile1% triethylamine (28：72)and a flow rate of 1.0 mL•min1.The Ex was set at 485 nm and Em was set at 546 nm. ResultsThe linear range of R123 was 15.625～500.000 ng•mL1 (r=0.999 7),with the lowest detectable limit at 5.000 ng•mL1.The recovery of extraction was (83.05±2.71)% .Both the intraday RSD and interday one were less than 8%. The sample showed a good stability within 24 hours after extraction and three freezethaw cycling. ConclusionThe method is simple, sensitive, accurate and reproducible, and which is suitable for pharmacokinetic study of R123 in rat plasma.
  • 药物研究
    Experimental Study on Acute Toxicology of Salcalcitonin Buccal Spray in Mice
    ZHOU Shunchang;ZHU Youmei;DU Zuohua;HUANG Kaixun;ZENG Fandian
    2009, 28(6): 695-697. https://doi.org/10.3870/yydb.2009.06.004
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    （1. ABSTRACTObjectiveTo investigate the acute toxicology of salcalcitonin administered via muscle and sublingually for further chronic toxicity study. Methods90 cleaning KM mice were given with salcalcitonin buccal spray by muscle injection and tongue mucous with different doses, respectively. The acute reaction and the main viscera pathological morphology were evaluated by observing the fur, symptoms of central nervous system and mortality. ResultsLD50 in cleaning mice(♂+♀) of salcalcitonin by muscle injection was 55 780.24 U•kg1 (50 660.56－61 417.36 U•kg1).But LD50 in cleaning KM mice(♂) by muscle injection was 68 173.75 U•kg1 (52 372.73－88 742.05 U•kg1). Within 24 h of injection, all mice appeared astasia, abnormal action, restless, shivering and ataxia. The symptoms lasted till 96 h. No unexpected reaction was found in mice by of salcalcitonin buccal spray given via tongue mucous for four times, and the MTD of which was 10 000 U•kg1. Conclusion It shows that salcalcitonin i.m. presents certain acute toxicity, but via tongue mucous is more safe. The sexuality of mice impacts acute toxicity of salcalcitonin buccal spray.
  • 药物研究
    Hyperlipoidemia and Antiatherosclerosis Effects of Chitosan
    YUAN Juping;HUANG Jinhua;LU Yuan
    2009, 28(6): 698-699. https://doi.org/10.3870/yydb.2009.06.005
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    ABSTRACTObjectiveTo investigate the mechanism of antiatherosclerosis effects by chitosan．Methods40 rats were randomly divided into 4 groups：normal control, hyperlipidemia model, chitosan at low dosage and one at high doasge．After 9 week’s experiment, the total cholesterol(TC)，tryglyceride(TG)，low density lipoprotein(LDL), high density lipoprotein(HDL)， SOD and MDA in sera were detected, and pathomorphology of aorta was studied．ResultsCompared with those in the hyperlipidemia model rats，the levels of MDA and TC，TG in the sera of rats given with chitosan were lower, while the activity of SOD and level of HDL were increased．The aortic valves in rats fed with chitosan was significantly thinner and number of foam cells was less than those in the model rats．ConclusionThe chitosan could attenuate production of atherogensis by lowing levels of TG，TC，MDA and elevating HDL and SOD.
  • 药物研究
    Impact of Amifostine on the Result of MTT Assay
    GUO Zongming;LI Yu;LUO Wenda;ZHU Min;ZHANG Li;FENG Changwei;XU Tiao
    2009, 28(6): 700-701. https://doi.org/10.3870/yydb.2009.06.006
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    ABSTRACTObjectiveTo investigate the impact of amifostine(AMI) on the result of MTT assay. MethodsFour groups were arranged as: reset group(medium), control group(medium+cell)、AMI group(AMI+medium) and AMI+Cell group(AMI+medium+cell). At the point of MTT adding and 4 h later,the color of cell culture was observed and the A was detected by enzymelinked immune assay. ResultsThe results showed that the color of culture was unchanged in both reset group and control group, and the of which were low after adding MTT (0.087±0.021 and 0.093±0.018).Even incubated for 4 hours, the color and A still didn’ t change (0.076±0.015 and 0.103±0.022, P＞0.05); the color changed into amethyst in both AMI group and AMI+Cell group, and the A were 0.215±0.013 and 0.274±0.013 after adding MTT with AMI at 0.5 mmol•L-1 ,which were significantly higher than those in the reset and control group in a dosedependent way of AMI. After incubating for 4 hours, the A ceased increasing in the AMI group(0.247±0.031 ,P＞0.05),but continued to elevate (0.381±0.022， P＜0.05) in the AMI+Cell group. Conclusion It is concluded that AMI and its metabolites may directly react with MTT and change the color and value,impacting the result of MTT assay.
  • 药物研究
    Comparison of Relative Bioavailability between Disintegration Tablets and Capsules of Cefixime in Healthy Volunteers
    LONG Minghui
    2009, 28(6): 702-704. https://doi.org/10.3870/yydb.2009.06.007
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    ABSTRACTObjective To compare the pharmacokinetic parameters and relative bioavailability between disintegration tablets and capsules of cefixime. MethodsAn open, randomized, twoperiod crossover study was conducted in 20 healthy volunteers after a single dosage of 200 mg cefixime or reference one orally. Concentration of cefixime in serum was assayed by HPLC method. The pharmacokinetics parameters were calculated, and AUC, Cmax and tmax were analyzed by two onesided t test. ResultsThe AUC, Cmax and tmax of disintegration tablets and capsules were (19.438±9.400) and (21.220±9.751) mg•h•L1， (2.614±0.931) and (2.811±1.030) mg•L1， (3.499±0.596) and (3.314±0.825) h, respectively. The main pharmacokinetics parameters of cefixime between two groups were corresponding (P＞0.05). The relative bioavailability of cefixime disintegration tablets was (110.14±12.70) %. ConclusionThe disintegration tablets and capsules of cefixime are bioequivalent.
    • 中药及天然药物专栏
  • 中药及天然药物专栏
    Antifatigue Effects of Polysaccharides with Different Relative Molecular Weight from Porphyra Haitanensis
    ZHAO Tingting;ZHANG Quanbin;LI Zhien;XU Zuhong
    2009, 28(6): 709-712. https://doi.org/10.3870/yydb.2009.06.010
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    (1. ABSTRACTObjectiveTo investigate the antifatigue effects of polysaccharides with different relative molecular weight （P1,P2,P3） from Porphyra haitanensis and the natural one（P）. MethodsMice were given with porphyrans for two weeks.The time of forcedswimming and pole climbing, and liver glycogens after forcedswimming test were detected. Results The degraded products increased liver glycogens, and significantly prolonged the time of forcedswimming and pole climbing in a molecular dependent manner as P1＞P2＞P3. The natural product P, elevated liver glycogen and prolonged the time of pole climbing as well. ConclusionThe degraded porphyrans are more effective in antifatigue function and synthesis of glycogens than the natural one.
  • 中药及天然药物专栏
    Preliminary Study on Serum Pharmacochemistry of Traditional Uighur Medicine Vernonia Anthelmintica(L.) Willd
    LIU Xiaodong;YAN Ming;ZHANG Lanlan;HUO Shixia
    2009, 28(6): 713-715. https://doi.org/10.3870/yydb.2009.06.011
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    (1. ABSTRACTObjective To explore the bioactive constituents of Vernonia anthelmintica(L.)Willd for its serum pharmacochemistry research. MethodsThe blood migrating constituents of Vernonia anthelmintica(L.)Willd were determined by comparing the HPLC fingerprints of the aqueous extracts, drug contained sera and blank sera. ResultsNine compounds absorbed into blood were detected, five among which were original constituents of Vernonia anthelmintica(L.)Willd, the rest might be metabolites of the original ones. ConclusionThese nine constituents absorbed in blood could be bioactive components of Vernonia anthelmintica(L.)Willd. Further studies on which would be useful to clarify the bioactive constituents and mechanisms of action of Vernonia anthelmintica(L.)Willd.
  • 中药及天然药物专栏
    Therapeutic Effects of Erigeron on Stable Effort Angina in Canine
    LU Zhuolin;XU Xiangwei;HE Xiaoyun;ZHAO Zhuanyou;ZHOU Zewei
    2009, 28(6): 715-718. https://doi.org/10.3870/yydb.2009.06.012
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    (1. ABSTRACTObjectiveTo study the therapeutic effects of erigeron on a stable effort angina pectoris in dogs. MethodsThe dogs were subjected to left anterior descending coronary artery (LAD) stenosis to generate a stable effort angina pectoris only when the hearts were paced. The effect of erigeron was evaluated by observing changes of STsegment on electrocardiograms, coronary flow and coronary resistance. ResultsSTsegment descended rapidly only when the the dogs were subjected to left anterior descending coronary artery (LAD) stenosis and hearts were paced. Nitroglycerin obviously inhibited this change. The pathological model was proved to be reliable. Erigeron injected through vein reduced STsegment descending by pacing and lowered the coronary resistance.Conclusion Erigeron could relieve the stable effort angina pectoris in a prevention regime.
  • 中药及天然药物专栏
    The Antitumor Effect of Polysaccharides from Eucommia Ulmoides Oliv in Mice
    XIN Xiaoming;WANG Dawei;ZHAO Juan;WANG Hao;FEI Hongrong;DONG Feng;GAO Yunsheng
    2009, 28(6): 719-721. https://doi.org/10.3870/yydb.2009.06.014
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    (1. ABSTRACTObjectiveTo study antitumor effect of polysaccharide from eucommia ulmoides (EUP). MethodsThe inhibitory effect of eucommia polysaccharide on Sarcomal 180 (S180) in mice was studied. The negative and positive control mice were treated with physiologic saline and cyclophosphamide (CP), respectively. The antagonism effects of eucommia polysaccharide on adverse reaction caused by CP on mice was studied as well. Results10.9 g of polysaccharide was extracted from 500 g eucommia ulmoides, the purity of which was 55.2%. Eucommia polysaccharide inhibited the growth of tumor S180, increased the index of spleen and thymus in mice. It also elevated the leukocyte count of peripheral blood and bone marrow, and reversed the reduction of leucocyte count induce by cyclophosphamide. ConclusionThe polysaccharide from eucommia ulmoides has the antitumor effect. It could improve the immunity and resist the bone marrow depression induced by cyclophosphamide.
  • 中药及天然药物专栏
    Effect of Guanxinning Injection on the QT Dispersion in Old Patients with Pneumocardial Disease Complicated with Coronary Artery Disease
    WANG Ling;YANG Rongshi;ZENG Yulan;XIONG Ping;LIU Xia
    2009, 28(6): 722-723. https://doi.org/10.3870/yydb.2009.06.015
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    ABSTRACTObjectiveTo explore the effect of guanxinning injection on the QT dispersion in old patients with pneumocardial disease complicated with coronary artery disease.Methods106 old eligible patients were divided into two groups. The control group was (53 cases) given with regular treatments. The test group (53 cases) was intravenously infused with 20 mL of guanxinning injection per day for 14 days, in addition to regular treatments. The changes of QT dispersion were observed in two groups. ResultsSignifciant changes of the QT dispersion after treatment were found in the test group ［(78.70±19.72) ms versus (48.30±20.63) ms, P＜0.01］, while, no significant changes were observed in the control group (P＞0.05). ConclusionGuanxinning injection can reduce the QT dispersion in pneumocardial disease complicated with coronary artery disease.
    • 药物与临床
  • 药物与临床
    Effects of Atorvastatin on Prognosis of Transient Ischemic Attack
    ZHAI Zhiyong;NIE Yingxue;LI Jiashu;ZHANG Chaodong
    2009, 28(6): 739-742. https://doi.org/10.3870/yydb.2009.06.026
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    （1. ABSTRACTObjectiveTo study the effects of statins on prognosis of transient ischemic attack. Methods459 patients had transient ischemic attack (TIA) within one to six months, with lowdensity lipoprotein (LDL) cholesterol levels of 2.51－4.89 mmol•L-1, and without known coronary heart disease were enrolled and treated with 60 mg of atorvastatin (220 cases) per day or placebo (239 cases)in a doubleblind way. All the patients were followedup for 1 a. The primary end point was a firstepisode nonfatal or fatal stroke. ResultsThe mean LDL cholesterol level of atorvastatin group and control group after a year was 2.28 mmol•L-1 and 3.34 mmol•L-1, respectively(P＜0.05). 36 patients (16.4%) receiving atorvastatin and 59 patients (24.7%) receiving placebo had a fatal or nonfatal stroke (P＜0.05). 30 cases in the atorvastatin group had cerebral infarction and 6 cases developed hemorrhagic strokes, whereas 53 cases in the placebo group had cerebral infarction and 6 cases in which developed hemorrhagic strokes. The incidence of cerebral infarction in atorvastatin group decreased significantly in comparison with that of the placebo group(P＜0.05), whereas the incidences of hemorrhagic stroke did not significant deviate in two groups(P＞0.05). The risk of major cardiovascular events in the atorvastatin group was decreased obviously(P＜0.01). The overall mortality rates were similar, with 7 deaths in the atorvastatin group and 5 deaths in the placebo group.ConclusionAtorvastatin at a dose of 60 mg•d-1 reduced the overall incidence of strokes and cardiovascular events in patients with TIA and without known coronary heart disease.
    • 药物制剂
  • 药物制剂
    Study on the Macroporous Resins for Isolation and Purification of Cornus Officinalis Saposins
    LI Xinru;ZHAO Feng;LONG Wei;LIU Peixun
    2009, 28(6): 767-770. https://doi.org/10.3870/yydb.2009.06.040
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    ABSTRACTObjectiveTo explore the optimal type of macroporous resins for isolating and purifing Cornus officinalis saposins. MethodsThe comphrehensive evaluation of the optimal type among HPD300 resin、AB8 resin and SA2 resin with both static and dynamic adsorptions was conducted, and the best one screened out was used to purify Cornus officinalis saposins. ResultsIt was showed that resin HPD300 had better adsorption properties for Cornu officinalis saponins. ConclusionThe macroporous resins are useful to isolate and purify total saposins in Cornus officinalis.The HPD330 resins is more suitable for purifying Cornus officinalis saposins.
  • 药物制剂
    Preparation and PhysicalChemical Characteristics of Nimodipine Liposome
    LU Jihong;LI Xudong;ZHANG Xiaoping;GAO Huayin
    2009, 28(6): 771-773. https://doi.org/10.3870/yydb.2009.06.041
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    （1. ABSTRACTObjectiveTo prepare nimodipine liposome and investigate the physicalchemical characteristics of the products. MethodsOptimized the formulation and preparation procedure of nimodipine liposome by using singlefactor design and orthogonal experiment design, and detected the entrapment efficiency, particle size and oxidation of the product. ResultsThe ratio of phospholipid to cholesterol in the optimal formulation of nimodipine liposome was 1：4，distearyl to the mixture of phospholipid and cholesterol was 1：10,the main drug to the total lipids was 1：40. The mean diameter of nimodipine liposome was 145.2 nm and the envelop rate was 97.6%.ConclusionThe product nimodipine liposome has a high entrapment efficiency and good stability, which fits for the clinical use.
  • 药物制剂
    Extraction, Purification and Determination of Polysaccharides from Cultured Mycelia of Cordyceps Militaris
    CHEN Lei;YANG Chunqing;SUN Mingshu
    2009, 28(6): 774-776. https://doi.org/10.3870/yydb.2009.06.042
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    ABSTRACTObjectiveTo establish a method for extraction, purification and determination of polysaccharides from cultured mycelia of Cordyceps militaris. MethodsThe best condition for extraction of polysaccharides was confirmed by orthogonal experiment, phenolsulphuric acid was used to determine the contents of polysaccharides, and Sephadex G100 was used to purify polysaccharides by molecular weight. ResultsThe whole concentration of polysaccharides was 1.36% by water extraction and EtOH precipitation; five compositions from polysaccharide CP1 was obtained by gelfiltration; and the main monosaccharide of polysaccharide CP1 was identified as glucose by being hydrolyzed with sulphuric acid. ConclusionThe method is simple, reproducible,accurate and reliable, which is fit for extractration, purification and determination of polysaccharides in cultured mycelia of Cordyceps militaris.
  • 药物制剂
    Preparation and in Vitro Release Profile of Retinoic Acideluting Stents
    DU Lingling;LIU Zongjun
    2009, 28(6): 776-778. https://doi.org/10.3870/yydb.2009.06.043
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    (1. ABSTRACTObjectiveTo develop a method for preparing retinoic acideluting stents(RAESs) and investigate their release behavior in vitro. MethodsThe retinoic acid layer and polymer layer were prepared at the content of 10 and 7 mg•mL1（W/V）,respectively, and were coated on the bare metal stents by the airspraying method; The release kinetics of RAES in vitro were carried out in 20% methonol solution at 37 ℃, the drug percent and layer thickness in retinoic acid layer were adjusted .ResultsRAESs prepared with only 1 mL 30% retioncid acid layer solution，or with 1 mL 50% retioncid acid layer solution followed by 0.5 mL polymer layer，could consistently release over 30 d. ConclusionRAESs prepared in two ways above have a good controlrelease and mechanical functions,which is suitable for further animal experiments.
    • 药品质量控制
  • 药品质量控制
    Contents Determination of Gentiopicroside in Gentiana Rigescens from Different Habitas in Guizhou Province
    HAN Shuang;LI Wei;XI Xianrong
    2009, 28(6): 783-785. https://doi.org/10.3870/yydb.2009.06.046
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    ABSTRACTObjectiveTo determine the content of gentiopicroside and evaluate the qulity of Gentiana rigescens from different habitats in guizhou province. MethodsHPLC was used with Alltima C18 (4.6 mm×250 mm ,5 μm), methanolwater(30：70) as mobile phase, detector wavelength at 270 nm and column temperature at 30 ℃.The optimal extraction method for gentiopicroside was sdudied by orthogonal experiments. ResultsThe calibration curve was linear with gentiopicroside in the range of 0.08～0.64 mg•mL1（r=0.999 9）, the average recovery was 102.4% with RSD of 1.69%（n= 5）.The contents of gentiopicroside in Gentiana rigescens from 11 habits were between 2.113%～4.808%. ConclusionThe quality of Gentiana rigescens from different habitats of guizhou province are consistent with the chinese Pharmacopoeia 2005, and there is no notable difference among them
    • 用药指南
  • 用药指南
    Stability and Compatibility of Potassium Sodium Dehydroandrogrographilide Succinat Injection with Ceftazidime Injection
    CHEN Fuchao;WAN Lin;FANG Baoxia;LI Peng;LI Qin;CHEN Qinhua;ZHU Jun
    2009, 28(6): 794-796. https://doi.org/10.3870/yydb.2009.06.052
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    (1. ABSTRACTObjective To study the stability and compatibility of potassium sodium dehydroandrographilide succinate injection with ceftazidime injection. MethodsHPLC method was adopted with the HypersilC18 as chromatographic column, acetonitrile0.02 mol•L1 potassium dihydrogen phosphate(pH 3.5) as gradient elution.The contents and pH value were determined after mixing of potassium sodium dehydro and rographolide succinate with ceftazidime injection in 0.9% sodium chloride under the room temperature within 8 hours,and the appearance of the solution was observed. ResultsNo significant differences were found in the appearance outside and the contents inside of the mixed solution, but the pH value increased gradually along with the time variation. ConclusionThe mixture of potassium sodium dehydroandrographilide succinate with ceftazidime injection in 0.9% sodium chloride were stable within 3 hours．
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