中国科技论文统计源期刊　中文核心期刊  
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第七届湖北十大名刊提名奖  

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    药物研究
  • 药物研究
    Construction of Myocardial Fibrosis Cell Model and Study the Therapeutic Effect of Valsartan
    JIANG Mei-hua;TU Ling;XU Xi-zhen;ZHAO Gang;WU Rui
    2008, 27(7): 739-742.
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    Objective To construct the cell model of myocardial fibrosis（MF）, investigate the therapeutic effect of valsartan on which, and provide a foundation for further research. MethodsCardiac fibroblasts( CFs) were isolated by collagenase digesting. The MF cell model was constructed with the addition of angiotensin Ⅱ(Ang Ⅱ) into the cells at the 3rd or 4th generation with a final concentration of 10-6μmol•L-1. The growth condition of CFs was observed and recorded under the light microscope at 4,6,8,12 and 24 h after drug intervention. The viability of CFs was quantitatively assessed by MTT assay and AO/EB staining. Transmission electron microscopy was used to observe the subcellular structure of cells. ResultsThe CFs began to adhere to the bottom 3 hours after plantation. The growth rate of the CFs was increased significantly with Ang Ⅱ . Morphological alteration like apoptosis was showed in part of cells within 24 hours of Valsartan’s addition. The most powerful inhibition on CFs was determined by valsartan at a concentration of 10.00 μmol•L-1 after 8 hours of treatment by MTT assay. It was also suggested that CFs showed the highest mortality rate under this condition by AO/EB staining method, and the main cause of death was apoptosis. The results drawn form transmission electron microscopy also indicated that apoptosis was the major feature of cell death. ConclusionThe cell model of MF could be constructed successfully under the available condition. Moreover, the CFs proliferation could be inhibited by valsartan for inducing the cell apoptosis.
  • 药物研究
    Penetration Acceleration Effects of Borneol for Clindamycin Across Blood Brain Barrier in Mice
    CHU Jian-bo;WANG Wei;ZHAO Wen-xia;SHANGGUAN Ying-ying
    2008, 27(7): 743-745.
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    Objective To establish a method for simultaneous determinating clindamycin in brain tissues by HPLCMS, and study the penetration assiting effects of borneol at different doses for clindamycin across blood brain barrier(BBB) in mice. Methods Mice were i.g. 0,150,300,600 mg•kg-1 of borneol (dissolved with PEG400), and i.v. 40 mg•kg-1 of clindamycin. The brain samples were taken out 15,30,45 min later, respectively. The homogeneous tissue was extracted with alkalined ethyl acetate and condensed with N2. Clindamycin were determined by means of selected iron extraction through double channels, m/z 425.20［M+H］+ for clindamycin and m/z 337.30［M+H］+ for fentanyl citrate as internal standard. Results The concentration of clindamycin in brain tissues could be determined accurately in this way. The concentration of clindamycin in brain was showed significant difference with that in the contrast group, with i.g. 300 mg•kg-1 of borneol 30 min after. Conclusion The method is quick, accurate and specific. And the borneol could assist the penetration of clindamycin across BBB in Mice.
  • 药物研究
    Effect of Gel Matrixes and Enhancers on the Permeability of Lappaconite Hydrobromide through Human Skin
    LI Xin-ping;ZHONG Yun-wei;WU Zheng;GAO Jian-qing
    2008, 27(7): 745-748.
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    Objective To study effects of gel matrixes and penetration enhancers on the permeability of lappaconite hydrobromide through human skins．MethodsCarbomer 934 and HPMC were used as gel matrixes and mixed with azone or oleate to prepare lappaconite hydrobromid gel. The absorbance of lappaconite hydrobromide through human skin was evaluated by using the TK-12A type diffusion cells. Results The absorption of lappaconite hydrobromide was better by using carbomer 934 as matrix than HPMC. 3% azone plus 20% ethanol significantly enhanced the drug absorbing from carbomer gel through human skin. However, 3% oleate had no effect on drug absorption. Conclusion Lappaconite hydrobromidcontaining gel with carbomer 934 as matrix and 3% azone plus 20% ethanol as permeability enhancer shows nice drug transdermal delivery effects.
  • 药物研究
    Protection of N-acetylcysteine on Kidneys of Diabetic Rats
    WANG Juan;JIA Ru－han;YANG Ding－ping;LIU Hong－yan;DING Guo－hua
    2008, 27(7): 748-751.
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    Objective To observe effects of N-acetylcysteine on kidneys of diabetic rats. Methods 14 SD rats were i.p. injected with streptomycin(STZ) 60 mg•kg-1 and divided into two groups: the diabetic model group, the treatment group, which treated with N－acetylcysteine(125 mg•kg-1•d-1), and another 7 normal rats as negative control. Kidney indext/body weight, blood glucose(BS), TC、TG、UAE、Ccr for 24 h were measured after 8 weeks. Meanwhile, MDA and TAOC were measured by a commercial kit. The glomerular structure was observed by PPAS staining and light microscopy. In addition, the expression of desmin in glomeruli were determined by immunohistochemistry. Results TC、UAE、Ccr、MDA in the treatment group were decreased significantly in comparison to the diabetic model group; however, TAOC increased markedly. The renal pathologic changes of the treatment group were improved as well, compared with those in the model group. Compared with the diabetic group, the desmin expression was reduced significantly in the treated group. Conclusion N－acetylcysteine delays the process of diabetic nephropathy, which could be related to attenuating glomeruli oxidative stress and,preventing podocyte damage.
  • 药物研究
    Effect of Ceftazidime on Pharmacokinetics of Netilmicin on Subjects
    WANG Xiao－hua;WANG Xiao－gang;XIA Chun－hua
    2008, 27(7): 751-754.
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    Objective To study effects of ceftazidime on pharmacokinetics of netilmicin on subjects and provide information for its rational use in clinical practice. Methods The serum and urine netilmicin at different time point were determined by a high performance liquid chromatographyindirect photometry method in 12 subjects given with netilmicin by drop infusion or netilmicin combined with ceftazidime. The pharmacokinetic data of netilmicin and urine recoveries were calculated with DAS software. Results The pharmacokinetics of netilmicin both in single use or combined with ceftazidime fitted with an open twocompartment model. AUC0-t, t1/2β,CL of netilmicin and the urine recovery within 024 h from a single administration group were (52.93±5.58) mg•L-1•h, (3.68±0.33) h, (4.49±0.53) L•h-1 and 72.22%,while the data from coadministration group were (71.81±8.03) mg•L-1•h, (5.06±0.57)h, (2.95±0.37) L•h-1 and 59.20%, respectively. It was indicated that there was a significant difference in the parameters between two groups. Conclusion The elimination of netilmicin from human bodies descended markedly when combined with ceftazidime, which resulted in drug accumulation after continuous administration. A proper reduction in dosage of netilmicin was necessary when combined with ceftazidime.
    • 中药及天然药物专栏
  • 中药及天然药物专栏
    Establishment of Polymorphism Reaction for Amplified Fragment Length and Silver-staining System for Polygonum multiflorum Thunb
    YAN Ping;GUO Jie－wen;JIAO Xu－wen;PANG Qi－Hua;ZHAO Xuan;ZHAO Shu－jin
    2008, 27(7): 757-760.
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    Objective To establish the optimum polymorphism (AFLP) analysis system for amplified fragment length and silver staining system for Polygonum multiflorum Thunb. Methods The improved method of CTAB－DNA isolation was used to extract total DNA from Polygonum multiflorum Thunb. Several factors including the quantity and time for digested DNA were investigated and orthogonal test was used to optimize the system of preamplification and selective amplification. Results 400 ng DNA were digested with restriction enzymes at 37 ℃ about 5 h. The sharp fingerprint patterns were obtained by 6% denatured acrvlamide/bisacrvlimade gels electrophoresis with the AFLP silverstaining using the optimized system of pre－amplification and selective amplification. Conclusion The AFLP reaction system could be used to identify the species and local populations for Polygonum multiflorum Thunb.with efficiency, strong stability and reliability.
  • 中药及天然药物专栏
    Effect of Zhishi Xiaopi Pill on Blood Gastrin and Motilin in Rats
    ZENG Rong;LI Jing－yun
    2008, 27(7): 760-762.
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    Objective To investigate the influence of different dosages of zhishi xiaopi pill(ZSXPP) on blood gastrin(GAS) and motilin(MTL) in rats. Methods 50 rats were randomly divided into five groups(n=10), normal group treated with distilled water, high, middle and low dosage of ZSXPP groups (7.5,15.0 and of 30.0 g•kg-1 of crude plants, respectively) and positive(10 mL•kg-1domperidone1 of domperidone) control group. After 4 weeks of treatment. the serum contents of gastrin and motilin were detected by radioimmunoassay. Results Contents of GAS and MTL in ZSXPP treated rats were significantly increased (P＜0.05), compared with those in the normal group . Conclusion ZSXPP could promote gastric motivity, which may be associated with increasing the contents of GAS and MTL in the blood.
  • 中药及天然药物专栏
    Determination of Paeoniflorin in Radix Paeonia hybrida from Different Habitats
    YANG Yuan-yuan;MA Xiao-kang;GU Zheng-yi;ZHOU Gang;WANG Yu-qing
    2008, 27(7): 763-764.
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    Objective To determine the contents of paeoniflorin in Radix Paeonia hybrida from 10 different habitats．MethodsAn Agilent TCC18 column (4.6 mm×150 mm,5 μm) was used with the mobile phase being methanol0.05 mol•L-1 KH2PO4 solution (34.8：65.2,V/V,flow rate at 1.0 mL•min-1,detecting wavelength as 230 nm, and the loading volume as 10 μL．ResultsThe linear range was 0.022 4  0.448 0 mg (r=0.999 9, n=6), and the average recocery of paeoniflorin was 98.8%～101.5%, with RSD as 0.355%1.770%(n=9). Conclusion Paeoniflorin from plants in 10 different habitats were accurately determined, and which all meet up the pharmacopoeia standards.
  • 中药及天然药物专栏
    Antioxidant Activity of Polyphenolic Constituents from Smilax china
    ZHAO Zhong-xiang;JIN Jing;FANG Wei;RUAN Jin-lan
    2008, 27(7): 765-767.
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    Objective To study the antioxidant activity of polyphenolic constituents from the traditional Chinese medicine Smilax china, and provide theoretical evidence for its traditional application. Methods TEAC assay and DPPH method were used to evaluate the free radical scavenging activity of nine polyphenolic compounds SCE1～9, which were isolated from the ethyl acetate soluble fraction of the rhizomes of Smilax china,and to preliminarily study the structureeffect relation of them. Results Compounds SCE1～9 showed strong antioxidant activity, since all of them could effectively scavenge free radicals like ABTS•+ and DPPH. In which the compound SCE1 had the highest antioxidant activity, with the TEAC value as 3.11±0.04 mmol•L-1, and the DPPH free radical scavenging rate was (95.95±0.76)% under the concentration of 50 μmol•L-1. Conclusion Polyphenolic constituents might be the main active part of rhizomes of Smilax china.
  • 中药及天然药物专栏
    Anti-rotavirus Effects of the Saponin and Volatile Oil from Psidium Guajava Leaves
    HUANG Hai-jun;ZHOU Ying-chun;YAN Wen;CHENG Bing-jie;HUANG Gui-qiong
    2008, 27(7): 772-775.
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    Objective To evaluate antirotavirus effects of the saponin and volatile oil from psidium guajava leaves by MTT assay. Methods The saponin and volatile oil were extracted from psidium guajava leaves,and added into MA104 cells. Afterwards, the cell viability was detected by MTT assay. Calculating TC50, different dose of the two components were added into rotavirus infected cells to determine their antirotavirus effects in vitro. Results It was found that the two components inhibited the infection of MA104 cells by rotavirus, the maximum suppression ratio were 62.12% and 60.04%, respectively for prophylactic use, the therapeutic index TI were 1.69 and 1.50. Meanwhile, the two components have therapeutical effects on rotavirus infected cells, the maximum inhibition rate were 78.37% and 86.55%, and the TI were 3.30 and 3.59, respectively. The antivirus effects showed a dose related manner. Conclusion The saponin and volatile oil from psidium guajava leaves have strong antirotavirus effects in vitro.
  • 中药及天然药物专栏
    Identification of Rhizoma Smilacis Glabrae by FTIR Spectroscopy Ordination Assay
    HUANG Mei-ai;CHEN Xiao-long;CHENG Cun-gui
    2008, 27(7): 775-777.
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    Objective To establish the method for quality control of rhizoma smilacis glabrae ( the slice of the root of Smilax glabra Roxb.). Methods FTIR of rhizoma smilacis glabrae and their confusable varieties were obtained by horizontal attenuated total reflectance of Fourier transform infrared spectroscopy (HATR-FTIR). The analysis of main components (PCA) was carried out by taking powder as comparing standard, selecting thirtyone representative absorbency data as a basis. Results PCA of FTIR exhibited practical value for identifying the degree of difference in chemical compositions among different species from the same genera. Conclusion The method of combing PCA with HATRFTIR for the quality control of rhizoma smilacis glabrae is simple, rapid and accurate.
  • 中药及天然药物专栏
    Pharmacological Experiments on Guangpu Zhixie Ye
    LIU Song;TAN Da-qi;LU Xiao-cong;LI Wei-xing;LI Qiu-hua;YAN Chun-hai
    2008, 27(7): 778-781.
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    Objective To observe the main effects of guangpu zhixie ye. Methods Anti-diarrhea and immunity enhancing effects of the drug was investigated by experiments as :① diarrhea induced by Rhubarb in mice, ,Rotavirus in Tupaia and pathogenic E.coli in Rabbit ; ②intestine impelling & stomach empty tests in mice ; ③immunity test in mice and in vitro antibacterial tests. Results Guangpu zhixie ye significantly reduced the diarrhea frequency in spleen deficiency mice induced by Rhubarb and in Tupaia by Rotavirus, prolonged the survival time of rabbits remarkably, inhibitted intestine impelling and stomach empty, and improved the phagocytic index of peritoneal macrophage and specific antibody forming ability in mice. Moreover, the drug also showed an excellent broadspectrum bacteriostasis effect in vitro. Conclusion Guangpu zhixie ye has antidiarrhea effects, the mechanism underlying might be related to inhibiting the gastrointestinal motility of smooth muscle.
    • 药物与临床
  • 药物与临床
    62 Cases of Hyperthyroidism with Leukopenia Treated with the Complex Zaofan Pill
    YU Jun-ping;WANG Ji-ru;FANG Ming
    2008, 27(7): 791-792.
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    Objective To determine the clinical effectiveness of the complex zaofan pill on patients with hyperthyroidism and leukopenia. Methods 116 hyperthyroidism patients with leukopenia were randomly divided into the treatment group (n＝62 )and control group (n＝54). The treatment group was given 9 complex zaofan pills, po,tid;the control group was given leucogen 20 mg,tid and Vit B4 20 mg,tid. The leukocyte was mornitored after 2 courses of treatment (2 weeks a course ). Results The effective rate was higher in the treatment group than that in the control group (83.87% vs 61.11%, P＜0.05). The increase of total leukocytes was more obvious in the treatment group than that in the control group. Slight stomach uncomfort occasionally appeared in the treatment group. Conclusion The complex zaofan pill is benefitful for treating patients with hyperthyroidism and leukopenia, which deserves clinic expending.
    • 药物制剂
  • 药物制剂
    Entrapment Efficiency Determination of Tetramethylpyrazine Phosphate Nanoliposome
    YAN Yi-lin;ZHOU Li-ling;LIN Shao-yu;HUANG Xiong
    2008, 27(7): 824-825.
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    Objective To establish a method for determining entrapment efficiency of tetramethylpyrazine phosphate(TMPP) nanoliposome. Methods The free TMPP were separated from liposome by Sephadex G-50 gel column, and the entrapment efficiency was determined by ultraviolet spectrophotometry．Results Free TMPP could be effectively separated from liposome by sephadex chromatography. The column recovery of TMPP and the recovery of the TMPP sample were 99.09% and 98.35%, respectively.The linear range was 0.160～24.048 μg•mL-1 (r＝0.999 8), the entrapment efficiency of TMPP nanoliposome was 36.12%, RSD=1.18%. Conclusion The method is simple and accurate, which can be used for determining entrapment efficiency of TMPP nanoliposome.
    • 药品质量控制
  • 药品质量控制
    Content Determination of Dexibuprofen in the Gel Preparation by HPLC
    WANG Wen-qing;WANG Qiu-lan;FANG Jian-guo;MA Yong-gui;TANG Jie;SHI Chun-yang
    2008, 27(7): 835-837.
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    Objective To establish a method for content determination of dexibuprofen in the gel preparation by HPLC. Methods Hypersil ODS2 column was used, with acetonitrilewater (pH 3.0, 58：42) as mobile phase, detecting wavelength as 263 nm, flow rate as 1.0 mL•min-1 and temperature as 35 ℃. Results The dexibuprofen showed a good linear correlation with a range of 25.1～251.0 μg •mL-1,r=0.999 9. The average recovery was 101.6% （n=9）. Conclusion This method is simple, rapid, accurate and repeatable, which can be used for quality control in this preparation.
  • 药品质量控制
    Establishment of Quality Standard for Jianpiyangwei Granules
    ZHAO Hong-zhi;YE Ting-xiang;LIU Zheng-hui;CHENG Yi;LIU Xin-yuan;LI Chun
    2008, 27(7): 837-840.
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    Objective To establish the quality standard for jianpiyangwei granules. Methods Glycyrrhetinic,Rhizoma Atractylodis Macrocephalae and Radix Codonpsis were identified by TLC. Contents of magnolol and honokiol were determinated by HPLC. The chromatographic column was an Agilent Eclipse XDB C18 (4.6 mm×250 mm, 5 μm), the mobile phase was methanolwater (65：35), the detection wavelength was 294 nm, with the flow rate at 1.0 mL•min-1, and the column temperature at 30 ℃. Results Glycyrrhetinic,Rhizoma Atractylodis Macrocephalae and Radix Codonpsis could be detected by TLC. The linear ranges of magnolol and honokiol were from 57.04 to 570.40 ng (r= 0.999 7) and from 37.76 ng to 377.60 ng (r=0.999 5), respectively. The average recoveries of samples were 97. 7% (RSD as 1.0 %) and 97. 0 % (RSD as 1.9%) , respectively. Conclusion The method is specific, simple, fast and accurate, which can be used for quality control of jianpiyangwei granules.
  • 药品质量控制
    Studies of the Quality Control for Shengfa Capsule
    2008, 27(7): 841-843.
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    Objective To establish the quality standard for shengfa capsule. Methods The thin liquid chromatography was used to identify the botanical drugs and the high performance liquid chromatography was applied to determine contents of 2,3,5,4′-tetrahydroxys tilbene-2-O-β-Dglucoside in shengfa capsule. Results Fleeceflower root, Glossy privet fruit and Alisma L. orientale Juzep could be identified by the established standard. The average content of 2,3,5,4′tetrahydroxys tilbene-2-O-β-Dglucoside was 0.256 mg per capsule. The average recovery ratio and relative standard deviation were 97.61% and 1.27%, respectively. Conclusion The quality standard is easy and simple for manipulating and can be used for the quality control of Shengfa capsule.
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