药物研究
LIU Yunhai;XIE Wei;FANG Jianguo;LI Jing
2006, 25(1): 0-0.
ObjectiveTo probe into the effect of the antiendotoxic part in Radix Isatidis on the expression of moesin mRNA in tissues of mice induced by lipopolysaccharide(LPS).MethodsOne week before the test, an intraperitoneal injection of 0.2 mL·（20 g）-1 bacillus calmettcguerin(BCG) was given to each of 30 mice, which were then randomized equally into 5 groups. Mice of the test groups were given 0.4 mL·（20 g）-1 of 1.00%,0.50% and 0.25% solution of F022 by gastrogavage , mice of the control group were given 0.4 mL·（20 g）-1 of 1.00% solution of F022 by gastrogavage , and mice of the model group were given each an equal volume of 0.9% sodium chloride. The above procedures were repeated 1.5 h later, and 30 min following the second drug administration, the test groups and the model group were given 0.2 mL·（20 g）-1 of LPS. Mice were anesthetized 9 h later by ether with the liver, kidney and spleen tissues treated by moesin mRNA hybridization in Situ and the staining of cytoplasm observed under microscope. ResultsA brown yellow staining of the tissue cytoplasm was taken as positive, and the administration of LPS could increase moesin mRNA expression .With prior administration of F022 part in radix isatidis the moesin mRNA expression by LPS was inhibited with a dose dependent characteristic. ConclusionThe F022 part in radix isatidis can inhibit the LPS induced moesin mRNA expression in tissues of mice liver, kidney and spleen with an antiendotoxic mechanism possibly at the molecular level.
