中国科技论文统计源期刊　中文核心期刊  
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊  
日本科学技术振兴机构数据库（JST）
第七届湖北十大名刊提名奖  

Archive

  • Select all
    |
    Contents in Brief
  • Contents in Brief
    Dynamic Observation of Maixuekang Capsule on the Expression of MMP-2 and TIMP-2 in Rats with Unilateral Ureteral Obstruction
    GUO Qingxi, PU Xia, CHEN Kaifeng, CAO Ling, YANG Chengwan, SUN Xingwang
    2015, 34(1): 3-6. https://doi.org/10.3870/yydb.2015.01.001
    Download PDF ( )   Knowledge map   Save
    Objective To study the effect of maixuekang caspsule on the expression of matrix metalloproteinases-2 (MMP-2) and tissue inhibitors of metaloproteinases-2 (TIMP-2) in the occlusion kidney dynamically by unilateral ureteral obstruction (UUO) in rats, and to investigate the effect and the mechanism of maixuekang caspsule on the renal interstitial fibrosis (RIF). Methods The model rats were induced by ligating the right ureter.Ninety-six male Sprague Dawley (SD) rats were randomly divided into normal control group, sham-surgery group, model control group and maixuekang group (0.05 g·kg－1·d－1 by gastric gavage started 3 days prior to operation).Rats were sacrificed on the 1th, 4th, 7th and 14th day after UUO was initiated.The immunohistochemical technique and image analysis technique were performed to examine the expression of MMP-2 and TIMP-2 in each group. Results On day 4 after UUO, the expression of MMP-2 decreased in both model control and maixuekang groups, but the expression was significantly higher in maixuekang group compared with the model control group (P＜0.05).The expression of TIMP-2 was raised in both model control and maixuekang groups, but the expression was significantly lower in maixuekang group compared with the model control group (P＜0.05).On day 14 after UUO, the MMP-2/TIMP-2 ratio decreased in both model control and maixuekang groups, but the ratio in maixuekang group (0.892±0.158) was significantly higher than the model control group (0.595±0.138, P＜0.05). Conclusion Maixuekang caspsule regulates the metabolism of extracellular matrix to postpone the proceeding of RIF, by eliciting the expression of MMP-2 and down-regulating the expression of TIMP-2.
  • Contents in Brief
    Influence of Different Extraction Technologies on Hypoglycemic Activity of Hubei Green Tea Polysaccharide in Vitro
    JIA Liangliang,XI Wei,PENG Guanliang,ZENG Xiao,CHEN Jinbing,JIN Guilan
    2015, 34(1): 7-10. https://doi.org/10.3870/yydb.2015.01.002
    Download PDF ( )   Knowledge map   Save
    Objective To investigate the influence of different extraction methods on hypoglycemic activities of polysaccharides from Hubei green tea. Methods Different green tea polysaccharides were extracted by enzymatic extraction, hot water extraction or cold water extraction, respectively.The inhibitory activities of the extract on α-glucosidase, α-amylase and invertase in vitro were investigated to determine the most efficient extraction technique. Results There was no significant difference in the inhibition of α-glucosidase by polysaccharides from three different extractions.The polysaccharides extracted by enzyme method were more active on α-amylase and invertase than those extracted from hot water and cold water methods. Conclusion The green tea polysaccharides extracted by enzyme method showed better hypoglycemic activity in vitro.
  • Contents in Brief
    Effects of Grape Seed Proanthocyanidin Extract on Spatial Working Memory and 5-Hydroxytryptamine in the Rat Model of Intermittent Hypoxia
    LIU Wenqian,ZHAO Yaning,LI Jianmin
    2015, 34(1): 11-14. https://doi.org/10.3870/yydb.2015.01.003
    Download PDF ( )   Knowledge map   Save
    Objective To investigate the effects of grape seed proanthocyanidin extract (GSPE) on 5-hydroxytryptamine (5-HT) and spatial working memory after intermittent hypoxia in rats. Methods Eighty male Sprague Dawley (SD) rats were divided randomly into four groups (n＝20 in each group):the normal control, model control, GSPEs at small (100 mg·kg－1) and high (200 mg·kg－1) dose groups.Rats in the normal control group were exposed in air and the model controls suffered from intermittent hypoxia (50 mL·L－1, 8 hours daily for 6 weeks).The spatial memory ability of rats in each group was assessed with the Morris water maze (MWM).The morphological changes of neuronal cell were observed by HE staining.The concentration of 5-HT in brain was detected by high performance liquid chromatography. Results Compared with the normal control group, the MWM test showed that the latency was prolonged and the frequency of crossing the platform was higher than that in model control group (P＜0.05).The survival cells and the concentration of 5-HT significantly decreased in the model control group (P＜0.05).Compared with the model control group, MWM test showed that the escaping latency was shortened and the frequency of crossing the platform was increased in the GSPE treatment groups compared with the model control group (P＜0.05).The above observations are more significant in GSPE high dose group that the low dose group.The survival cells and the concentration of 5-HT significantly increased in the GSPE treatment groups (P＜0.05). Conclusion GSPE can regulate the concentration of 5-HT and improve learning memory function under intermittent hypoxia.
  • Contents in Brief
    Comparison of Pharmacokinetics Between Recombinant Liver Microsomes with CYP3A4 and CYP3A29
    XUE Zhengkai, WEI Hong
    2015, 34(1): 15-21. https://doi.org/10.3870/yydb.2015.01.004
    Download PDF ( )   Knowledge map   Save
    Objective To compare the pharmacokinetic characteristics of microsomes from the recombinant cell lines of HepG2 with CYP3A4 and CYP3A29 in Bama miniature pigs, and provide evidence for clinical animal testing. Methods The probe drugs nifedipine (NF), testosterone (TST) and ketoconazole (KCZ) were incubated with the recombinant microsomes of HepG2-CYP3A4 and HepG2-CYP3A29 under optimal conditions of concentration and duration.The high performance liquid chromatograph (HPLC) was utilized to detect the metabolites, and characteristics of the two types of microsomes were compared. Results There was no significant difference in the metabolic activities between CYP3A29 and CYP3A4 for both nifedipine and testosterone (P＞0.05).The half inhibitory concentrations of ketoconazole for CYP3A29 and CYP3A4 were 0.090 and 0.132 μmol·L－1 (P＜0.05), respectively, using nifedipine as the metabolism substrate, and were 0.056 and 0.032 μmol·L－1(P＜0.05), respectively, using testosterone as metabolism substrate. Conclusion There is no significant difference between cell microsomes with CYP3A29 and CYP3A4 in metabolizing nifedipine and testosterone, but ketoconazole has significantly different inhibitory activity towards the two types of microsomes.
  • Contents in Brief
    Protective Effect of Dexmedetomidine on Acute Lung Injury Induced Sepsis in Rats
    YANG Peng,HOU Jun,XU Qingguo,CHEN Chun,TANG Heqing,KE Qibin
    2015, 34(1): 22-25. https://doi.org/10.3870/yydb.2015.01.005
    Download PDF ( )   Knowledge map   Save
    Objective To investigate the protective effect of dexmedetomidine (Dex) on septic lung injury in rats. Methods One hundred male SD rats were randomly divided into four groups (n＝25 in each group).Group Ⅰ was treated with sham operation only.Groups Ⅱ, Ⅲ, and Ⅳ received cecal ligation and puncture (CLP) to create the sepsis model.Group Ⅱ served as model control, group Ⅲ and Ⅳ received intraperitoneal injections of Dex at 12.5 and 25.0 μg·kg－1 , respectively, at 0,2,4,and 6 hours after CLP.TNF-α and IL-6 in lung tissue were measured at 0,2,4,6, and 24 h.ELISA was used to detect the levels of HMGB1, TNF-α and IL-6 at 24 h.The expression of TLR4 protein was assessed by western blotting.The acute lung injury was detected by HE stain and W/D ratio. Results In groups Ⅲ and Ⅳ, the expressions of TNF-α and IL-6 were decreased significantly compared with group Ⅱ(P＜0.05).The expression of TLR4 in lung tissue and W/D ratio were decreased in groups Ⅲ and Ⅳ compared with group Ⅱ(P＜0.05).There was no significant difference between group Ⅲ and Ⅳ. Conclusion Dexmedetomidine can attenuate lung inflammatory reaction and lung injury in septic rats, the mechanism of which may be related to suppression of TLR4 pathway.
  • Contents in Brief
    Protective Effects of Genipin on Diabetic Nephropathy in Mice
    ZHANG Hongxia
    2015, 34(1): 26-29. https://doi.org/10.3870/yydb.2015.01.006
    Download PDF ( )   Knowledge map   Save
    Objective To explore the kidney protective effects of genipin on diabetic nephropathy (DN) in mice. Methods Thirty Kunming mice were randomly divided into normal control group, polyethylene glycol (PEG) group and genipin group (n＝10).The diabetic mice model was established by intravenous injections of streptozotocin.The body weight, blood glucose concentration, kidney superoxide dismutase (SOD) activity and malondialdehyde (MDA) were detected.Urinary albumin, tumor necrosis factor-α (TNF-α) and serum interleukin-6 (IL-6) levels in kidney were measured by ELISA method.The expression of nuclear factor-κB( NF-κB), p65, inducible nitric oxide synthase (iNOS) and caspase-3 were detected by Western blotting. Results In the normal control, PEG and genipin groups, the level of urinary albumin was (11.40±0.98),(120.30±9.57) and (56.20±4.07) mg, respectively.Serum creatinine was (54.3±9.7) , (130.6±21.3) and (87.3±10.1) μmol·L－1, repectively .The renal SOD activity was (2 430±1 010), (2 260±370), and (3 060 ± 400) U·L－1, respectively.The renal TNF-α level was (683.50±54.88) , (961.40±39.52) and (531.70±15.84) pg·mg－1, respectively.Serum IL-6 level was (74.40±16.70) , (686.60±12.38) and (196.30±62.50) pg·mg－1, repectively.The expression of NF-κBp65, iNOS and caspase-3 in the kidney of genipin group were all decreased(P＜0.01). Conclusion Genipin protects kidneys in DN mice through antioxidation and anti-inflammation effects.
  • Contents in Brief
    Preparation of LHRHa and R8 Co-modified 5-FU Loaded Liposome and Its Anti-prostate Cancer Effect
    YANG Qingliang, XIA Yonghua, HE Yan, ZHANG Guangyun, SONG Ge
    2015, 34(1): 30-34. https://doi.org/10.3870/yydb.2015.01.007
    Download PDF ( )   Knowledge map   Save
    Objective To prepare luteinizing hormone-releasing hormone analogues (LHRHa) and R8 co-modified 5-FU loaded liposome (LHRHa/R8-LP-5-FU) and investigate therapeutic effect towards prostate cancer. Methods The co-modified liposome was prepared by film-ultrasonic method.The appearance, particle size and Zeta potential were evaluated.The cellular uptake by PC-3 cells in vitro was used to evaluate the targeting efficiency.The anti-proliferation activity of LHRHa/R8-LP-5-FU was evaluated by MTT assay.Tumor spheroids were used to evaluate anti-tumor ability of LHRHa/R8-LP-5-FU in vitro. Results The particle diameter of the co-modified liposome was (115.0±15.2) nm with the Zeta potential of (11.00±2.15) mV.The embedding ratio of LHRHa/R8-LP-5-FU was (84.5±5.1)%.The result demonstrated that the ratio of co-modified liposome uptaken by PC-3 were 2.8 and 3.2 times higher than that of R8-LP and LHRHa-LP(P＜0.01), respectively, and the cell inhibition of LP-5-FU, R8-LP-5-FU, LHRHa-LP-5-FU and LHRHa/R8-LP-5-FU were (26.4±4.5)%, (39.5±4.2)%, (48.6±3.5)% and (82.5±4.3)% (P＜0.01), respectively.The inhibition of the growth of tumor sphere by LHRHa/R-LP-5-FU was obviously higher than that of the other liposome groups. The experimental results of cell toxicity were consistent with the experimental results of cell uptake. Conclusion The co-modified liposome may serve as a promising prostate cancer delivery system for antitumor agents.
  • Contents in Brief
    Inhibitory Effect of Ciprofloxacin Combined with Lactoferrin-derived Peptides Chimera on the Biofilm Formation and Production of the Signal Molecules of Quorum Sensing System in Pseudomonas aeruginosa
    ZUO Peng, WANG Aili,WANG Zhengyun,XU Guopeng, HU Qiongjie, SHAO Bing, XU Xilin, XIONG Weining,XIONG Shengdao
    2015, 34(1): 35-39. https://doi.org/10.3870/yydb.2015.01.008
    Download PDF ( )   Knowledge map   Save
    Objective To compare the inhibitory effect of single use of ciprofloxacin (CIP),lactoferrin-derived peptides chimera (LFchimera) and combination of CIP plus LFchimera on the biofilm formation and production of the signal molecules of quorum sensing(QS) system in Pseudomonas aeruginosa. Methods The wild type strain of P.aeruginosa-PAO1 was used as test strain,which was divided into the control group , CIP(0.04 μg·mL－1)group, LFchimera(0.25 μmol·L－1 and 1.00 μmol·L－1), CIP(0.04 μg·mL－1) plus LFchimera(1.00 μmol·L－1)one.The expression of biofilm and the signal molecules of QS in PAO1 were quantitatively determined respectively, and were compared among groups. Results Compared with those in the control group(1.511 3±0.031 8,0.502 5±0.028 3), the quantitative expression of biofilm and the signal molecules of QS in CIP group(1.073 3±0.010 9,0.245 3±0.014 0),LFchimera low dose group(1.065 5±0.011 4,0.235 9±0.010 7),LFchimera high dose group(0.665 3±0.012 9,0.108 6±0.007 0)and the combination group(0.122 1±0.013 0,0.048 2±0.005 4)were all significantly reduced (P＜0.05).The quantitative expression of biofilm and the signal molecules of QS had no significant differences between the CIP group and LFchimera low dose group(P＞0.05), were significantly lower in the LFchimera high does group compared to the LFchimera low dose group and the CIP group(P＜0.05), and were remarkably lower in the combination group compared to single drug groups (P＜0.05). Conclusion Both of CIP and LFchimera at sub-MICs could inhibit biofilm formation and the production of signal molecules of QS in Pseudomonas aeruginosa.The inhibition effect of LFchimera is dose-dependent.This inhibitory effect could be further enhanced when CIP is combined with LFchimera, which indicats they have synergistic effect on the resistance of biofilm formation in Pseudomonas aeruginosa by suppressing the production of the signal molecules of QS.
  • Contents in Brief
    Effects of the Active Efflux Gene on Multidrug Resistance of Acinetobacter Baumannii to Beta Lactam Antibiotics
    SUN Jingna,YANG Jizhang,WANG Guoxin,ZHAO Shuai,LIU Zeshi,ZHANG Zheng
    2015, 34(1): 40-42. https://doi.org/10.3870/yydb.2015.01.009
    Download PDF ( )   Knowledge map   Save
    Objective To investigate the effect of expression of active efflux gene on multidrug resistant Acinetobacter baumannii in the course of resistance to beta lactam antibiotics. Methods Drug resistance of 96 strains of multidrug resistant Acinetobacter baumannii to nine beta lactam antibiotics was analyzed.A total of 34 efflux phenotype positive strains were isolated according to the standard that the MIC level was reduced by more than 75 percent when the carbonyl cyanide chlorobenzene hydrazone inhibitors were added.The efflux pump protein gene adeB was detected, analyzed and sequenced by the method of PCR amplification. Results The antibiotic resistance rate to cefotaxime, cefiazidime,ceftriaxone,cefepime, cefperazone/sulbactam were 92.3%,86.6%,83.0%,70.8%,68.3%, respectively.The resistance rate to ampicillin, piperacillin, piperacillin/tazobactam and ampicillin/sulbactamare were 90.1%, 77.5%, 72.4%,67.3%, respectively.The adeB genes were detected in 33 stains out of 34 of the efflux pump phenotype positive strains, and the positive rate was 97.06%.The 33 strains were sequenced for adeB efflux pump gene.We found the sequence homology was 100.0% match with Genebank. Conclusion Active efflux pump gene in Acinetobacter baumannii plays an important role in multidrug resistance to beta lactam antibiotics.
  • Contents in Brief
    Risk Factor Analysis for Liver Injury Induced by Linezolid for Treatment of Methicillin-Resistant Staphylococcus Aureus Pneumonia
    PANG Chang, PU Chenghao, LIN Shuwu
    2015, 34(1): 43-46. https://doi.org/10.3870/yydb.2015.01.010
    Download PDF ( )   Knowledge map   Save
    Objective To study the safety of linezolid in treating the methicillin-resistant staphylococcus aureus (MRSA) pneumonia, and investigate the risk factors associated with liver injury. Methods Retrospective analysis was conducted in 50 cases of MRSA pneumonia treated with linezolid in first affiliated hospital to Chinese Medical University.Single factor analysis and non-conditional logistic regression analysis were used to analyze the possible risk factors for liver injury. Results The incidence of liver injury was 56.0%.Univariate analysis showed that there was no relationship between the patient's underlying liver function and liver injury ( χ2＝1.524, P＞0.05).However, there were associations between age ( χ2＝13.345,P＜0.01), gender ( χ2＝4.461, P＜0.05), duration of treatment ( χ2＝11.436, P＜0.01), combination of antibiotics ( χ2＝9.416, P＜0.01) and liver injury.Logistic regression analysis showed that age, gender, treatment duration and antibiotics combination are associated with liver function impairment. Conclusion The use of linezolid against MRSA pneumonia is safe in clinical use.The liver injury caused by treatment is reversible, but the risk factors should be concerned, and the liver function should be monitored.
       
  • Contents in Brief
    Cost-effectiveness of Antibiotic Therapy in Children with Purulent Meningitis
    DING Xiangyu, ZHANG Guying,LI Chengling, WANG Jianxin, SHANG Tao
    2015, 34(1): 47-49. https://doi.org/10.3870/yydb.2015.01.011
    Download PDF ( )   Knowledge map   Save
    Objective To explore the optimal treatment strategy with the lowest cost and best effect for children with purulent meningitis. Methods A total of 572 cases of children diagnosed with purulent meningitis were divided into 5 groups according to their antibiotics regimen(A:ceftriaxone sodium-ceftazidime, n＝222; B:ceftriaxone sodium-meropenem, n＝202; C:ceftazidime-meropenem, n＝52; D:ceftriaxone sodium-penicillin G sodium, n＝48; E:meropenem, n＝48).A cost-effectiveness analysis was performed under the principles of pharmacoeconomics along with decision tree model. Results The cost-effectiveness ratios of A,B,C,D and E groups were 101.25,109.50,117.49,123.44,188.02,respctievly.Group A had the lowest cost-effectiveness ratio. Conclusion Ceftriaxone sodium-ceftazidime is the optimal combination for the treatment of children with purulent meningitis .
  • Contents in Brief
    Establishment and Analysis of HPLC Fingerprint Eucommia Ulmoides Oliv.from Guizhou
    LIU Xing, ZHOU Xin,ZHOU Mei,CHEN Huaguo, GONG Xiaojian
    2015, 34(1): 84-88. https://doi.org/10.3870/yydb.2015.01.023
    Download PDF ( )   Knowledge map   Save
    Objective To establish the high performance liquid chromatography (HPLC) fingerprint of Eucommia ulmoides Oliv.and compare the contents of chemical components of Eucommia ulmoides Oliv.from different habitats,which provides a reference for the development of the quality control standards of Eucommia ulmoides Oliv. Methods The chromatographic separation was performed on a Phenomenex Synergi Hydro-RP C18 (250 mm×4.6 mm,4 μm) column with a mixture of methanol-0.05% phosphoric acid (gradient elution) as the mobile phase.The detection wavelength was set at 235 nm, and column temperature was kept at 25 ℃.The similarity was evaluated with the traditional Chinese medicine chromatographic fingerprint similarity evaluation software system.Hierarchical cluster analysis and principal component analysis were applied for the chemical fingerprint pattern recognition. Results The HPLC fingerprint of Eucommia ulmoides Oliv.was set up, 19 common peaks were determined, and three peaks were identified compared to reference substances.The similarity evaluation showed differences among twenty Eucommia ulmoides Oliv.samples from different areas, dividing them into classes A and B by the hierarchical cluster analysis. Conclusion The fingerprint of Eucommia ulmoides Oliv.provides evidences for the quality control evaluation of it.
  • Contents in Brief
    HPLC Fingerprint Analysis of Water-soluble Components in Danshen Capsules
    ZHANG Jun,CHENG Yuefa,ZHANG Aibing
    2015, 34(1): 89-94. https://doi.org/10.3870/yydb.2015.01.024
    Download PDF ( )   Knowledge map   Save
    Objective To establish a high performance liquid chromatography (HPLC) fingerprint of water-soluble components in danshen capsules. Methods The HPLC was performed on a Thermo Syncronis C18 column(250 mm×4.6 mm,5 μm) with methanol-acetonitrile-phosphoric acid in water as mobile phase in gradient elution at a flow rate of 1 mL·min－1, and the column temperature was kept at 30 ℃.The detection wavelength was 286 nm. Results Nine and 22 common peaks were selected as the HPLC fingerprints of water-soluble components in danshen capsules from two manufactures, respectively.The standard HPLC fingerprints of water-soluble components were established. Conclusion The analysis is stable and reproducible, which can be applied to the quality control for water-soluble components in danshen capsules.
  • Contents in Brief
    Simultaneous Determination of 5-Hydroxymethylfurfural and Phenolic Acids in Xiangdan Injection by RP-HPLC
    CHENG Qiaoyuan,GUO Zengxi, CHEN Bilian
    2015, 34(1): 95-98. https://doi.org/10.3870/yydb.2015.01.025
    Download PDF ( )   Knowledge map   Save
    Objective To establish a method for content determination of 5-hydroxymethylfurfural, sodium danshensu, protocatechuic aldehyde, rosmarinci acid and salvianolic acid B in xiangdan injection by reversed phase high performance liquid chromatography (RP-HPLC) . Methods The analysis was performed on a Kromasil C18(250 mm×4.6 mm,5 μm)column with acetonitrile-0.05% water solution of trifluoroacetic acid as mobile phase at the gradient elution mode with the flow rate of 0.8 mL·min－1.The column temperature was kept at 30 ℃ and the detector wavelength was set at 288 nm.The content was calculated with external standard method. Results The linear relationships between the concentration and peak area of 5-hydroxymethylfurfural, sodium danshensu, protocatechuic aldehyde, rosmarinci acid and salvianolic acid B were 0.002 73－1.364 00 μg(r＝1), 0.274 80－13.740 00 μg(r＝0.999 9),0.034 64－1.732 00 μg(r＝1.000 0), 0.053 59－2.680 00 μg(r＝1.000 0), and 0.105 30－5.264 00 μg(r＝1.000 0), respectively.The average recovery and the RSD of the five components were 100.93%(1.00%),101.36%(0.87%),100.54%(0.51%),105.14%(0.39%),and 102.31%(1.91%), respectively. Conclusion This method has been proven to be simple, fast, reliable and reproducibile, which can be applied to the content determination of the five components in xiangdan injection.
  • Contents in Brief
    Determination of Dehydrocavidine in Different Parts of Corydalis Saxicola Bunting from Various Origins by HPLC
    YE Yong,HUANG Qiujie,LIU Huagang
    2015, 34(1): 99-101. https://doi.org/10.3870/yydb.2015.01.026
    Download PDF ( )   Knowledge map   Save
    Objective To establish a quantification method for the determination of dehydrocavidine in Corydalis saxicola Bunting by reversed phase high performance liquid chromatography (RP-HPLC).Different parts of the herb from two different sources were analyzed in order to provide evidence for setting quality standards for Corydalis saxicola Bunting. Methods The samples were separated by RP-HPLC on a SHISEIDO-SPOLAR C18 column(250 mm×4.6 mm,5 μm), with the mixture of acetonitrile and buffer solution of 0.05 moL·L－1 sodium dihydrogen phosphate (25:75) as the mobile phase.The detection wavelength was set at 347 nm, and the flowing rate was 1.0 mL·min－1.The column temperature was kept at 30 ℃. Results The linear range of dehydrocavidine was within 0.024 1－0.482 0 μg(r＝0.999 9),and the average recovery was 99.48% with RSD of 1.44%(n＝6).There was great variation in the content of dehydrocavidine under different growing conditions.Wildly grown Corydalis saxicola Bunting had the highest content of dehydrocavidine, and the roots have the highest content among different parts of the plant. Conclusion The established method is simple, accurate, and reproducible, therefore can be applied to the quality control for analogous alkaloids in Corydalis saxicola Bunting.
  • Contents in Brief
    Quantitative Determination of Osthole in Supercritical CO2 Fluid Extracts of Cnidium monnieri by Gas Chromatography-Mass Spectrometry
    ZHANG Ren, CHEN Qinhua, ZHU Jun, YU fei
    2015, 34(1): 102-103. https://doi.org/10.3870/yydb.2015.01.027
    Download PDF ( )   Knowledge map   Save
    Objective To establish a method for content determination of osthole from Cnidium monnieri in supercritical fluid CO2 (SFE-CO2) extract by gas chromatography-mass spectrometry (GC-MS). Methods The sample was extracted from Cnidium monnieri by SFE-CO2 and solved by acetic ether.The content of ostholei determined by GC-MS with selected ion monitor(GC-MS/SIM).Separation was performed on DB-5MS capillary column (30 m×0.32 mm,0.25 μm) at the flow rate of 2.0 mL·min－1.The column temperature was started at 60 ℃ for 2 min, then increased to 280 ℃ at 10 ℃·min－1 and maintained at 280 ℃ for 6 min.The temperature of both interface and inlet was kept at 280 ℃. Results The linear range of osthole was within 5－1 000 μg·mL－1(r＝0.999 1).The mean extraction recovery was 98.97% and limit of detection was 1.0 ng·mL－1. Conclusion The method is reliable and stable,which can be applied to quality control of Cndium monnieri.
  • Contents in Brief
    Compatibility of Cefmenoxime Hydrochloride for Injection with Five Kinds of Infusions
    WANG Jianxin,YU Jing, LIU Ruiqin, YANG Jizhang, WANG Ru
    2015, 34(1): 108-110. https://doi.org/10.3870/yydb.2015.01.029
    Download PDF ( )   Knowledge map   Save
    Objective To study the stability of cefmenoxime hydrochloride for injection after mixing with fructose injection, invert sugar injection, invert sugar electrolyte injection, xylitol injection,or xylitol and sodium chloride injection. Methods The physical appearance,quantity of particles and pH were observed at room temperature after mixing cefmenoxime hydrochloride with each of above injections for 6 hours at concentrations simulated in clinical use.The content of cefmenoxime was determined by high performance liquid chromatography ( HPLC). Results There was no significant difference in appearance, pH or cefmenoxime content after cefmenoxine mixed with 5 kinds of infusions for 6 hours, and the quantity of particles met the requirement. Conclusion Cefmenoxime is stable when dissolved in all 5 kinds of infusions, which can be used as solvents for it.
  • Contents in Brief
    Pharmaceutical Care for a Patient with Acute Pancreatitis Complicated with Alcohol Withdrawal Syndrome
    LUO Hongli, XIAO Shunlin, LI Yan, YU Lei
    2015, 34(1): 116-119. https://doi.org/10.3870/yydb.2015.01.031
    Download PDF ( )   Knowledge map   Save
    Objective To explore the pharmaceutical care approaches for patients with acute pancreatitis by clinical pharmacists. Methods There were various contradictions when treating patients with acute pancreatitis complicated with alcoholic liver disease, alcohol withdrawal syndrome and pulmonary infections.In this patient case, clinical pharmacists worked with physicians to individualize medication regime and monitored the process of treatment. Results With the cooperation between clinical pharmacists and physicians,the symptoms of acute pancreatitis were improved,the pulmonary infection, the subsequent antibiotics-associated diarrhea and alcohol withdrawal symptoms were all relived, and the patient was discharged. Conclusion Patients with comorbidities or therapeutic dilemma are good targets for pharmacist intervention.Clinical pharmacists can cooperate with physicians to optimize therapy and implement pharmaceutical monitoring for patients, and to promote rational use of drugs.
  • Contents in Brief
    A Case Analysis of Non-Hodgkin's Lymphoma with Liver Injury Induced by Chemotherapy
    XU Weijia,CAI Lin,WU Xue,WANG Liu,MENG Xiaohui
    2015, 34(1): 120-122. https://doi.org/10.3870/yydb.2015.01.032
    Download PDF ( )   Knowledge map   Save
    Objective To provide reference for rational drug use in patients with lymphoma during chemotherapy. Methods Clinical pharmacists participated in the treatment of one case of malignant lymphoma with liver injuryed after chemotherapy.According to the analysis, the main causes of liver injury in this patient were hepatitis B virus (HBV) reactivation and drug induced hepatitis. Results The liver function of this patient gradually recovered with antiviral therapy. Conclusion Patients with lymphoma should be routinely monitored for HBV serum markers, HBV-DNA and liver function before and after chemotherapy.Comprehensive treatment should be given in time when necessary.
RSS