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Objective To explore the kidney protective effects of genipin on diabetic nephropathy (DN) in mice. Methods Thirty Kunming mice were randomly divided into normal control group, polyethylene glycol (PEG) group and genipin group (n＝10).The diabetic mice model was established by intravenous injections of streptozotocin.The body weight, blood glucose concentration, kidney superoxide dismutase (SOD) activity and malondialdehyde (MDA) were detected.Urinary albumin, tumor necrosis factor-α (TNF-α) and serum interleukin-6 (IL-6) levels in kidney were measured by ELISA method.The expression of nuclear factor-κB( NF-κB), p65, inducible nitric oxide synthase (iNOS) and caspase-3 were detected by Western blotting. Results In the normal control, PEG and genipin groups, the level of urinary albumin was (11.40±0.98),(120.30±9.57) and (56.20±4.07) mg, respectively.Serum creatinine was (54.3±9.7) , (130.6±21.3) and (87.3±10.1) μmol·L－1, repectively .The renal SOD activity was (2 430±1 010), (2 260±370), and (3 060 ± 400) U·L－1, respectively.The renal TNF-α level was (683.50±54.88) , (961.40±39.52) and (531.70±15.84) pg·mg－1, respectively.Serum IL-6 level was (74.40±16.70) , (686.60±12.38) and (196.30±62.50) pg·mg－1, repectively.The expression of NF-κBp65, iNOS and caspase-3 in the kidney of genipin group were all decreased(P＜0.01). Conclusion Genipin protects kidneys in DN mice through antioxidation and anti-inflammation effects.