中国科技论文统计源期刊 中文核心期刊  
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊  
日本科学技术振兴机构数据库(JST)
第七届湖北十大名刊提名奖  

Archive

  • Select all
    |
    药物研究
  • 药物研究
    JIANG Ming;ZHENG Qisheng;YU Guangyun;LONG Wei;LIU Peixun
    2014, 33(11): 1401-1406.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo explore the antitumor mechanism of xianhe yanling recipe(XHYLR).MethodsThe mechanism of action of 241 compounds contained in the Chinese medicine, XHYLR was studied by using computational pharmacological.Methods including molecular docking, biotechnology network, chemical space technology and the prediction of absorption, distribution, metabolism, excretion and toxicity (ADME/T).ResultsMost of the compounds in XHYLR had good druglike properties.Distribution of 241 compounds in chemical space also revealed that these compounds showed similar antitumor activity.It suggested that the prescription of XHYLR was reasonable.The phenomena that one molecular interacted with multiple target proteins and several molecules interact with one target protein were observed in XHYLR.For example, the COX2 interacted with 40 micromolecules, and catechin interacted with five targets including COX2.ConclusionThese results are helpful for understanding the complicated mechanism of antitumor activity of XHYLR.
  • 药物研究
    WU Yuanyuan;JIN Guimin;DU Guanhua;LYU Yang;DAI Guidong
    2014, 33(11): 1407-1411.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo study the pharmacokinetics difference of levofloxacin polymorphs in rats, evaluate the advantageous medical polymorph, and explore the effects of different polymorphs on clinical medicine.MethodsFour crystal forms of levofloxacin were administered intragastrically to rats,and high performance liquid chromatography (HPLC) was used to measure the contents of levofloxacin in rat plasma. The pharmacokinetic parameters were calculated and comparedResultsAfter a single oral dose, the peak plasma concentration (Cmax) of crystal forms of Ⅰ,Ⅱ,Ⅲ and Ⅳ of levofloxacin was 6.984, 9.692, 9.405, 6.424 mg•L-1; the time to peak (tmax) was 0.6, 0.9, 1.0, 1.0 h; the halflife (t1/2) was 4.207, 2.97, 4.857, 1.695 h; the area under the curve (AUC0→12 h) was 31.478, 42.385, 32.406, 31.636 mg•h•L-1.ConclusionThere is no statistically significant difference in pharmacokinetic parameters.However, compared with other crystal forms, plasma concentration of crystal form II is higher and maintained longer.Therefore, crystal form II of levofloxacin is an advantageous polymorph for medicine.
  • 药物研究
    JIN Yan;HUANG Shan;ZHAO Lei;PEI Xiaona
    2014, 33(11): 1411-1415.
    Download PDF ( )   Knowledge map   Save
    ObjectiveThe study aimed to investigate the best extraction process and the analgesia and antiinflammatory properties of total flavonoids from Euphorbia prolifera.MethodsThrough single factor experiment and orthogonal test,factors that affect the extraction yield of total flavonoids were studied,including the extraction time,solid to liquid ratio,ethanol concentration and extraction times.The mice models of ear edema induced by xylene and twisting induced by acetic acid were used to evaluate the analgesia and antiinflammatory effects of different extractions from Euphorbia prolifera.ResultsTotal flavonoids were extracted by the refluxing method,and the optimum conditions were extracting for 3 hours,solid to liquid ratio of 1:30,ethanol content of 60% in the solvent,and processed for 2 times.The highest extraction yield of total flavonoids from Euphorbia prolifera was 5.63%.The ethanol,ethyl acetate and nbutanol extracts decreased twisting and ear swelling in mice.ConclusionThe extraction for total flavonoids from Euphorbia prolifera is simple,efficient and reproducible.The ethanol,ethyl acetate and nbutanol extracts of total flavonoids have obvious analgesia and antiinflammatory effects.
  • 药物研究
    WU Yan;YANG Lan;ZHANG Yufei;YUAN Xiaohuan;CHU Yanhui
    2014, 33(11): 1416-1419.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo explore the mechanism and effects of basic fibroblast growth factor (bFGF) on skin wound healing.MethodsFibroblasts (FB) were isolated from normal skin and hypertrophic scar and cultivated by direct adherence method.FB were then treated with different concentrations of bFGF (0,0.1,1,10,100,1 000 ng•mL-1) and cultivated with serumfree medium for 72 hours.The proliferation and apoptosis of FB in each group were detected by cell counting and trypan blue staining.Content and gene expression of typeⅠand type Ⅲ collagen and fibronectin were determined by ELISA and RTPCR,respectively.ResultsbFGF promoted the proliferation of FB at low concentrations promoted apoptosis of FB at higher concentrations.The proliferation of FB from hypertrophic scar was slower than that from the normal skin.bFGF significantly inhibited type Ⅰ collagen production from hypertrophic scar FB but not from the normal skin.Moreover,bFGF upregulated fibronectin expression in the normal fibroblasts,but not in the hypertrophic scar.No change in type Ⅲ collagen expression and production was observed in FB from either source.ConclusionbFGF has differential effects and mechanisms on FB of the normal skin and hypertrophic scar,suggesting that bFGF may play a role in early phase of skin wound healing and scar formation.
  • 药物研究
    CHEN Fanghui;YANG Renze;LUO Xinhui;ZHONG Sheng;LI Zeling;ZENG Taohui;WEI Guilin
    2014, 33(11): 1420-1423.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo explore the protective effect of rosiglitazone on insulin resistance (IR) induced by high glucose in vascular endothelial cells and its possible mechanism.MethodsHuman umbilical vein endothelial cells(HUVECs) was divided into 3 groups: the normal control group cultivated in DEME medium with 5.5 mmol•L-1 glucose;the high glucose group (HG) cultivated in DEME medium with 33 mmol•L-1glucose for 24 h after the IR model was set up;the rosiglitazone group cultivated in DEME medium with 33 mmol•L-1glucose and 10 μmol•L-1 of rosiglitazone for 24 h after the IR model was set up.The cell viability,nitric oxide (NO),endothelin1 (ET1),mitochondrial membrane potential,reactive oxygen species (ROS),pIKK and IkBa protein levels were detected.ResultsCompared with the normal control,the cell viability,the level of NO and the mitochondrial membrane potential were decreased,levels of ET1 and ROS increased,pIKK expression was upregulated,and IκBα expression was downregulated in HG group (all P<0.01).Rosiglitazone reversed these changes in a timedependent manner (P<0.05).ConclusionRosiglitazone has the protective effect on insulin resistance induced by high glucose in vascular endothelial cells via inhibiting ROS/IKK signaling pathway.
  • 药物研究
    XIE Xin;ZHANG Lin;CHEN Shiyu;YANG Fang;ZHANG Lide
    2014, 33(11): 1423-1426.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo study the effect of different doses of Zhengan xifeng decoction on Raf1 mRNA and protein expression in the cardiovascular tissue of spontaneously hypertensive rats (SHR).MethodsA total of 50 male SHR,24 weeks old,were randomly divided into the model,low dose,medium dose,high dose of Zhengan xifeng decoction and the compound apocynum groups,10 in each group.Ten homologous male rats (WKY) served as the normal control group.After gavaged for 5 weeks,western blotting and RTPCR were used to detect the Raf1 protein and mRNA expression in the cardiovascular tissue,respectively.ResultsCompared with the model control group,both Raf1 protein and mRNA expressions significantly increased in all treatment groups (P<0.01).ConclusionThe Zhengan xifeng decoction can stimulate cell proliferation and inhibit cell apoptosis by upregulating the expression of Raf1.
  • 药物研究
    ZOU Xianghui;LIU Bocong;ZHUANG Donghong;ZHA Guangcai;WU Yunying
    2014, 33(11): 1427-1430.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo investigate the protective effect of 3hydroxybutyrate derivate (methyl3hydroxybutyrate and ethyl3hydroxybutyrate) on the apoptosis of PC12 induced by FBS deficiency.MethodsMethyl3hydroxybutyrate and ethyl3hydroxybutyrate were prepared by chemical degradation.PC12 cells were divided into different groups: medium with FBS as negative control,medium without FBS as positive control,medium with different concentrations of methyl3hydroxybutyrate or ethyl3hydroxybutyrate without FBS as sample groups.The shape and viability of cells in each group were analyzed by optical microscope and MTT.ResultsCompared with the negative control,cells in the positive control group demonstrated a large number of apoptosis,smaller and thinner morphology,and lower activity.However,the activity of cells was improved in the sample groups.Low concentration (0.01 and 0.001 mg•mL-1) of methyl3hydroxybutyrate and ethyl3hydroxybutyrate showed a protective effect on cell apoptosis,and 1 mg•mL-1 had the best protection effect.ConclusionHigh purity methyl3hydroxybutyrate and ethyl3hydroxybutyrate can be produced by chemical method,and both chemicals present good effect on protecting the PC12 from apoptosis.
  • 药物研究
    DU Kaixian;LUO Yu;ZHANG Yan;DONG Yan
    2014, 33(11): 1431-1433.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo study the effect of lincomycin hydrochloride on the intestinal mucosal barrier of mice.MethodsA total of 24 mice were equally and randomly divided into normal control group and model group.Mice in the model group were administered with 8 mg•kg-1•d-1 of lincomycin hydrochloride through gastric lavage for 3 days to establish the model of intestinal mucosa injury and alteration of intestinal flora in mice.The intestinal flora was tested,bacterial translocation was analyzed,and mucosa permeability was determined by measuring diamine oxidase (DAO) in plasma.ResultsLincomycin hydrochloride led to alteration of intestinal flora in mice after oral administration for three days in mice.The aerobes translocation rate was 16.7% and 52.3% in the normal and model groups,respectively (P<0.01).The anaerobes translocation rate was 8.3% and 68.2% in the normal and model groups,respectively (P<0.01).The plasma concentration of diamine oxidase was increased from (2.08±0.05) mg•mL-1 in the normal group to (7.18±0.10) mg•mL-1 in the model group (P<0.01).ConclusionLincomycin hydrochloride damages intestinal mucosal barrier in mice.
  • 药物研究
    LI Jianli;LIANG Wei;WU Honghai;HOU Yanning
    2014, 33(11): 1434-1438.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo investigate the protective effect and the mechanisms of 17βestradiol on ketamineinduced apoptosis on primary cultured rat cortical neurons.MethodsCortical neurons were primarily cultured for seven days,then divided into four groups : control group (treated with equal valume of DMSO),estradioltreated group (treated with 0.1 μmol•L-1 17βestradiol),ketaminetreated group (treated with 100 μmol•L-1 ketamine),ketamine plus 17βestradioltreated group( treated with 0.1 μmol•L-1 17βestradiol+100μmol•L-1 ketamine). The neurons were treated for 24 hours.The neuron viability was determined by MTT.Neuroapoptosis was measured by nuclear morphometry after Hoechest 33258 dying.Western blotting was performed to detect the expression levels of cleavedcaspase3 and Bcl2protein.ResultsThe neuron viability in the ketamine group was (54.02±7.78)%,significantly decreased from the control group,whereas ketamine plus 17βestradiol increased the cell viability to (88.09±6.54)%,significantly higher than the ketamine group.The neuroapoptosis rate in the ketamine group was (49.50±4.34)%,significantly increased from the control group,while that in the drug combination group was (15.74±3.40)%,significantly lower compared with the ketamine group.Meanwhile,the cleavedcaspase3 expression increased,and Bcl2 expression decreased remarkably after ketamine treatment,while which was reversed in the drug combination group.Conclusion17βestradiol can protect against ketamineinduced injury by inhibiting neuron apoptosis.
  • 药物研究
    BAI Jing;HU Lei;ZHANG Li;TIAN Chunyu;PANG Dequan;BO Haimei;HAN Shuying
    2014, 33(11): 1438-1441.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo investigate the therapeutic effects and mechanism of Artemisia argyi ferment substance on systemic Candida albicans infection.MethodsThe model of systemic Candida albicans infection was established in immunosuppressed mice.The model mice were randomly divided into the model control,Artemisia argyi ferment substance (AAFS) at different doses (100,200,and 400 mg•kg-1) and fluconazole group (20 mg•kg-1),30 mice in each.Mice in each treatment group were given therapeutic drugs by gavage for 5 consecutive days,twice daily.The survival of mice was determined 21 days after the model was set up.The serum levels of IFNγ and IL2 were determined by ELISA.The proliferation activity of T lymphocyte in the spleen was detected by MTT assay.The number of living fungi in liver and kidney tissues was counted.ResultsCompared with the model control,AAFS at middle and high doses and fluconazole significantly increased the survival rate of mice,the serum levels of IFNγ and IL2,and the proliferation activity of T lymphocyte in the spleen,but decreased the number of living fungi in tissues(P<0.01).Compared with low dose AAFS,middle and high doses of AAFS and fluconazole showed significantly different effect on each index (P<0.05 or P<0.01),but there was no difference among these groups (P>0.05).ConclusionAAFS at 200-400 mg•kg-1 has inhibitory effects on systemic Candida albicans infection in mice,the mechanism of which is related to increasing the proliferation of T lymphocyte in spleen and the levels of IFNγ and IL2 in serum.
  • 药物研究
    BI Renbing;GUO Deliang;PAN Dingyu;LIU Zhisu;HU Shouguo
    2014, 33(11): 1442-1445.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo investigate the effects of radiotherapy combined with resveratrol (Res) on proliferation,invasion and apoptosis of hepatoma cell line Bel7404.MethodsBel7404 cell line was treated with Res(25 μmol•L-1 ) combined with radiotherapy,then divided into four groups (group 1: the control;group 2: radiation at dose of 2 Gy;group 3: radiation at dose of 4 Gy;group 4: radiation at dose of 6 Gy).Cell proliferation and invasion were detected by MTT assay.Cell apoptosis were observed by fluorescence microscopy.Expressions of MMP2 and VEGF proteins were determined by Western blotting.ResultsCompared with the control group,cell proliferation and invasion were significantly inhibited,while cell apoptosis was increased in all radiation groups (P<0.05).ConclusionThe sensitivity of hepatoma Bel7404 cells to radiation can be enhanced by resveratrol.Radiation therapy combined with resveratrol can inhibit proliferation and invasion of hepatoma cells and increase the cell apoptosis,which may be related with the downregulation of MMP2 and VEGF proteins.
  • 药物研究
    BIAN Jiaming;ZHANG Mei
    2014, 33(11): 1446-1449.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo study the phosphorylation mode of extracellular regulated kinase (ERK) induced by acute and chronic morphine treatment on Chinese hamster ovary (CHO) cells expressed with μ opioid receptors.MethodsThe time course of ERK phosphorylation 1 h and 36 h after morphine exposure as well as naloxoneprecipitated withdrawal was detected by immunobloting.ResultsA transient enhancement of ERK phosphorylation was induced by 1 μmol•L-1morphine with the peak effect at 5 min (P<0.01),and the effect was dosedependent.No difference in ERK phosphorylation was found after 36h of treatment with 10 μmol•L-1morphine compared with the control.However,5 or 10 minnaloxone precipitation induced remarkable decrease in ERK phosphorylation compared with the control (P<0.01).ConclusionDifferent changes of ERK phosphorylation were found under acute and chronic morphine treatment and naloxone precipitation,indicating a compensation of ERK related pathway induced by μ opioid receptors.
  • 药物与临床
  • 药物与临床
    JIANG Li;CHEN Xiaoping
    2014, 33(11): 1468-1470.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo observe the effects of octreotide combined with enzyme supplementation in treatment of pain in chronic pancreatitis.MethodsFiftysix patients were divided into two groups.Thirtysix patients in the treatment group were treated with octreotide combined with enzyme supplementation, and twenty patients in the control group were treated with enzyme supplementation alone.The pain relief rate, analgesic requirement per day and average days in hospital were analyzed between the two groups.ResultsCompared with the control group,the treatment group presented better improvement of abdominal pain(P<0.05),less requirement of analgesics(P<0.05), and shorter duration in hospital(P<0.05).ConclusionOctreotide combined with enzyme supplementation is effective in alleviating pain and improving quality of life in patients with chronic pancreatitis.
  • 药物与临床
    PEI Qiang;ZHAO Xide;SANG Wenfeng
    2014, 33(11): 1470-1473.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo investigate the relationship among electrocardiogram QRS complex duration,left ventricular ejection fraction (LVEF),Nterminal probrain natriuretic peptide (NTproBNP) and their relation to New York Heart Association (NYHA) functional classification in patients with chronic heart failure (CHF),and observe the intervention effects of Shenfu injection (SFI) on patients with CHF.MethodsA total of 72 CHF patients were randomly assigned to treatment group (36 cases) and control group (36 cases).Patients in the control group received standard care,and those in the treatment group received standard care plus 1.0 mL•kg-1•d-1 of SFI in 5% glucose for two weeks.Before and after the treatments,the electrocardiogram QRS complex duration,LVEF,NTproBNP were measured and NYHA functional classification was evaluated.ResultsElectrocardiogram QRS complex duration was negatively correlated with LVEF and was positively correlated with NTproBNP and NYHA functional classification (P<0.05).After the treatment,the proportion of patients with NYHA class ⅠⅡ significantly increased and class ⅢⅣ significantly decreased in both groups.The treatment group was superior to the control group in reinstating cardiac function (P<0.05).After the treatment,electrocardiogram QRS complex duration and the levels of NTproBNP were significantly descended and LVEF were significantly ascended in both groups (P<0.05).The level of NTproBNP in the treatment group was significantly lower than in the control group (P<0.05),but electrocardiogram QRS complex duration and LVEF did not show significant difference.The prior and posttreatment difference in electrocardiogram QRS complex duration and LVEF was more obvious in the treatment group than in the control(P<0.05).ConclusionThe extension of electrocardiogram QRS complex duration is correlated with plasma NTproBNP elevation and cardiac dysfunction,which can be used as a reference for disease progression or medication efficacy.SFI is effective on CHF of the yangqi deficiency type.
  • 药物制剂与药品质量控制
  • 药物制剂与药品质量控制
    XIAO Ping;CHEN Jianwei;LI Xiang;LI Jin
    2014, 33(11): 1482-1486.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo establish a HPLC method for simultaneous determination of uracil,hypoxanthine,guanosine,thymine,and (R,S)goitrin in Isatidis Radix.MethodsThe determination was performed on a Hanbon Hedera C18 column(4.6 mm×250 mm,5 μm).The mobile phase consisted of acetonitrile(A) and water(B) with linear gradient elution at the flow rate of 1.0 mL•min-1.The column temperature was 30 ℃.Detection wavelength was 254 nm.ResultsThe linear range of uracil,hypoxanthine,guanosine,thymine,and (R,S)goitrin was 1.97-39.40 mg•L-1(r=0.999 9),1.25-50.00 mg•L-1(r=0.999 9),0.25-10.40 mg•L-1(r=0.999 6),2.84-56.70 mg•L-1(r=0.999 9),and 0.72-28.80 mg•L-1(r=0.999 8),respectively.The average recovery was 99.36% (RSD=0.91%),99.79% (RSD=1.12%),100.90% (RSD=1.71%),98.67% (RSD=1.50%),and 99.33%(RSD=0.94%),respectively.ConclusionThe method is simple,accurate,reliable,reproducible and sensitive,which can be used as an effective method for quality control of Isatidis Radix.
  • 药物制剂与药品质量控制
    LI Zhiping;ZHAO Xiqing;ZHAO Shiqing;ZHANG Hui;LIU Yan;XIAO Ruolei
    2014, 33(11): 1486-1490.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo prepare longcirculating temperaturesensitive liposomes with paclitaxel (LTSLP),develop methods for determination of paclitaxel,related substances and monostearoyl phosphatidylcholine (MSPC) in LTSLP,and the haemolysis of LTSLP in vitro.MethodsHPLCUV methods for paclitaxel content and related substances and HPLCCAD method for MSPC in LTSLP were established and validated.Spectrophotometric method was used to determine hemolysis in vitro.ResultsThere was a good linear relationship between peak area and concentration within the range of 60.39-181.17 μg•mL-1.Recovery and precision of the method for determination of paclitaxel content met the requirements.Specificity,sensitivity,and system suitability for related substances were consistent with requirements.There was a good linear relationship between peak area and concentration within the range of 1.5-50.0 μg•mL-1for the determination of MSPC with good specificity,sensitivity and recovery.Paclitaxel contents in three batches of selfprepared LTSLP were between 90.0% and 110.0%,single related substances were below 0.5% and total impurities were below 2.0%.There was almost no hemolysis in vitro.ConclusionThe methods for determining paclitaxel content,related substances and haemolysis can be used to assess the quality of LTSLP.Selfproduced LTSLP consistently meet the quality standards.
  • 药物制剂与药品质量控制
    LI Jianguang;HAN Songlin;ZHAO Dongsheng;ZHANG Haibo;GENG Jing;LI Xinxia
    2014, 33(11): 1491-1495.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo establish a method of simultaneously quantitative analysis for multicomponents by single marker (QAMS) of galuteolin,apigenin7glucoside,7methoxycoumarin,luteolin and apigenin in two species of Chamomiles from Xinjiang.MethodsBy using apigenin as the internal standard,the relative correction factors (RCF) of galuteolin,apigenin7glucoside,7methoxycoumarin and luteolin were set and determined by UPLC.The contents of galuteolin,apigenin7glucoside,7methoxycoumarin,luteolin and apigenin in 11 samples of Chamomiles were authentically determined by the external standard method,to verify the accuracy of QAMS.ResultsThere is a good linear relationship within the range of standard curve for five compounds by the external standard method (r>0.999 3);the average recovery was 99.7%,97.5,98.8%,100.9%,99.1%,for galuteolin,apigenin7glucoside,7methoxycoumarin,luteolin and apigenin,respectively,with RSD as 1.8%, 0.6%, 0.5%, 1.8% and 1.9%,respectively.The RCF for pigenin7glucoside,galuteolin,7methoxycoumarin and luteolin to apigenin was 1.441,2.308,1.117,1.490,respectively,with RSD as 0.07%,0.04%,0.09% and 0.18%,respectively.No significant difference between the quantitative results by QAMS and external standard method was observed.ConclusionIt is practical to determine content of galuteolin,apigenin7glucoside,7methoxycoumarin,luteolin with RCF and apigenin by using external standard method,for the deficiency and expensive of the standard reference.
  • 药物制剂与药品质量控制
    FANG Xiaqin;LIU Mengmeng;ZHANG Xiuli;WU Yifei;ZHENG Wensheng
    2014, 33(11): 1496-1498.
    Download PDF ( )   Knowledge map   Save

    ObjectiveTo establish a method for determination of imatinib mesylate liposome and related substances.MethodsThe liquid chromatography was carried out on a Kromasil C18 column.The mobile phase A consisted of methanoloctane sulfonate solution (42:58).The mobile phase B consisted of methanoloctane sulfonate solution (4:96).The flow rate of gradient elution was 1.2 mL•min-1.The detection wavelength was 268 nm.The column temperature was room temperature.ResultsThe intermediates and degraded substances could be seperated under the selected chromatographic conditions.Imatinib mesylate showed a good linear relationship within 1-100 μg•mL-1,r=0.999 1(n=5).ConclusionThe method is specific,accurate,sensitive,and simple,and can be used for quality control of imatinib mesylate liposome.

  • 药物制剂与药品质量控制
    JIANG Yi;CHEN Jingjing;TIAN Feng;YANG Min
    2014, 33(11): 1499-1501.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo establish a headspace GC method for the determination of residual organic solvents in testosterone cypionate.MethodsThe residual organic solvents were determined by GC with DBWAX capillary colum(30 m×0.32 mm,0.25 μm) and FID detector at 230 ℃,using high purity nitrogen as the carrier gas by headspace sampling.The flow rate was 1.5 mL•min-1,the split ratio was 20:1,the temperature of injection port was 220 ℃,the headspace heating temperature was 70 ℃,and the headspace balance time was 40 mins.The content of residues was calculated by using npropanol as the internal standard.ResultsFour residual solvents were completely separated.There were good linearity for methanol,ethanol,benzene and pyridinein in the ranges of 4.17-2.50×103 μg•g-1 (R=0.999 9),4.18-2.51×103 μg•g-1 (r=0.999 6),0.84-172 μg•g-1 (r=0.998 1),and 2.95-1.77×103μg•g-1 (r=0.999 9),respectively.The detection limit was 2.08,1.23,0.28,and 0.87 μg•g-1,respectively.The average recovery of methanol,ethanol,benzene and pyridinein was 102.2%(RSD=4.0%),99.6%(RSD=1.9%),112.6(RSD=5.6%),and 98.9%(RSD=1.6%),respectively.ConclusionThis method is reliable,sensitive,accurate and can be used for the determination of residual organic solvents in testosterone cypionate.
  • 药物制剂与药品质量控制
    LIU Zegan;HUANG Liangyong;ZHU Haitao;DU Shiming
    2014, 33(11): 1502-1505.
    Download PDF ( )   Knowledge map   Save
    ObjectiveTo investigate the stability of jinyin qingre oral liquid.MethodsAccording to the stability test guidelines for pharmaceutical preparations,the appearance,pH and relative density of jinyin qingre oral liquid were detected,the contents of geniposide,chlorogenic acid and salvia acid B were determined by HPLC.The chromatographic conditions were as follows: AtlantisT3 column (150 mm×4.6 mm,3 μm),mobile phase A: 0.05% phosphoric acid,mobile phase B:acetonitrle,gradient elution at the flow rate of 0.8 mL•min-1,and column temperature at 35 ℃.The detection wavelength was set at 254 nm.ResultsThe appearance,pH,and relative density of jinyin qingre oral liquid did not change significantly with the acceleration test,but the contents of geniposide,chlorogenic acid and salvia acid B decreased time dependently.ConclusionLight and high temperature could decrease the stability of the jinyin qingre oral liquid.