ObjectiveTo establish a method for determination of imatinib mesylate liposome and related substances.MethodsThe liquid chromatography was carried out on a Kromasil C18 column.The mobile phase A consisted of methanoloctane sulfonate solution (42:58).The mobile phase B consisted of methanoloctane sulfonate solution (4:96).The flow rate of gradient elution was 1.2 mL8226;min-1.The detection wavelength was 268 nm.The column temperature was room temperature.ResultsThe intermediates and degraded substances could be seperated under the selected chromatographic conditions.Imatinib mesylate showed a good linear relationship within 1-100 μg8226;mL-1,r=0.999 1(n=5).ConclusionThe method is specific,accurate,sensitive,and simple,and can be used for quality control of imatinib mesylate liposome.