中国科技论文统计源期刊 中文核心期刊  
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊  
日本科学技术振兴机构数据库(JST)
第七届湖北十大名刊提名奖  

Archive

  • Select all
    |
    特约稿
  • 特约稿
    GAO Feng;CAI Qin;CAI Lun;LU Fu-rong
    2014, 33(10): 1261-1264. https://doi.org/10.3870/yydb.2014.10.001
    Download PDF ( )   Knowledge map   Save
    4-Hydroxyisoleucine (4-HIL) is the active component isolated from the seeds of Trigonella foenumgraecum.It exerts broad pharmacological effects such as antihyperglycemia, blood lipid lowing, improvement of insulin resistance, etc.As the Chinese traditional medicine, it can be used clinically in treating many diseases such as type 2 diabetes mellitus, dyslipidemia, metabolic syndrome, fatty liver and other diseases.Here the current research status and application prospect on 4-Hydroxyisoleucine were summarized.
  • 药物研究
  • 药物研究
    GUAN Hong-jing;CUI Chang-ping;HUANG Ji-you;SHU Fen;
    2014, 33(10): 1265-1268. https://doi.org/10.3870/yydb.2014.10.003
    Download PDF ( )   Knowledge map   Save
    Objective To investigate the effects of apigenin on the migration of vascular smooth muscle cells (VSMC) induced by platelet derived growth factor (PDGF)-BB and the possible molecular mechanism. Methods VSMCs were isolated from thoracic aortas of male Sprague-Dawley rats using enzyme digestion method.Migration of VSMCs was determined by transwell assay.Western blotting was carried out to evaluate phosphorylation of c-jun Nterminal kinase (JNK).Results Treatment with PDGF-BB (20 ng.mL-1) significantly promote VSMC migration, the number of migrated cells was 2.46 times than that of control group.However, after 12.5 μmol.L-1 apigenin pretreatment, the number of migrated cells was 46.5%of the PDGF-BB group.Various dose of apigenin can significantly inhibit VSMC migration induced by PDGF-BB , 12.5 μmol.L-1 apigenin treatment significantly inhibited PDGF-BB phosphorylation of JNK.Conclusion Apigenin can suppress the migration of VSMC induced by PDGF-BB . These beneficial effects on VSMC were at least partly mediated by the inhibition of activity of JNK.
  • 药物研究
    DAI Jing;WANG Li-cong;WU Dan;TU Sui-ping;QIU Li-ying
    2014, 33(10): 1269-1273. https://doi.org/10.3870/yydb.2014.10.004
    Download PDF ( )   Knowledge map   Save
    Objective To evaluate the inhibition effects of shuanghuanglian injection powder and its active components on the activities of CYP1A, CYP 2D and CYP 3A in rats liver microsomes. Methods Three probe substrates including phenacetin for CYP1A, dextromethorphan for CYP2D and midazolam for CYP3A were incubated with shuanghuanglian injection powder and the active components (baicalin, phillyrin, forsythiaside A, lutin, chlorogenic acid, coffeic acid and lutiolin) in rat liver microsomes.Contents of three probe substrates were simultaneously determined by HPLC to evaluate the metabolic rates.Results Shuanghuanglian injection powder and baicalin inhibited the activities of CYP2D and CYP 3A, but didn’t affect CYP1A.The other active components showed no effect on CYP1A, CYP2D and CYP3A.Conclusion Drugdrug interactions may occur when combining shuanghuanglian powder injection with CYP2D and CYP 3A substrates and baicalin may be the effector substance responsible for the interactions.
  • 药物研究
    DAI Jing;WANG Li-cong;WU Dan;TU Sui-ping;QIU Li-ying
    2014, 33(10): 1269-1273. https://doi.org/10.3870/yydb.2014.10.004
    Download PDF ( )   Knowledge map   Save
    Objective To evaluate the inhibition effects of shuanghuanglian injection powder and its active components on the activities of CYP1A, CYP 2D and CYP 3A in rats liver microsomes. Methods Three probe substrates including phenacetin for CYP1A, dextromethorphan for CYP2D and midazolam for CYP3A were incubated with shuanghuanglian injection powder and the active components (baicalin, phillyrin, forsythiaside A, lutin, chlorogenic acid, coffeic acid and lutiolin) in rat liver microsomes.Contents of three probe substrates were simultaneously determined by HPLC to evaluate the metabolic rates.Results Shuanghuanglian injection powder and baicalin inhibited the activities of CYP2D and CYP 3A, but didn’t affect CYP1A.The other active components showed no effect on CYP1A, CYP2D and CYP3A.Conclusion Drugdrug interactions may occur when combining shuanghuanglian powder injection with CYP2D and CYP 3A substrates and baicalin may be the effector substance responsible for the interactions.
  • 药物研究
    YU Qi-jing;TAO Hong;YANG Yun-zhao
    2014, 33(10): 1273-1277. https://doi.org/10.3870/yydb.2014.10.005
    Download PDF ( )   Knowledge map   Save
    Objective To investigate roles of superoxide dismutase-1(SOD1), phosphatidylinositol 3 kinase (PI3K)/serine/threonine protein kinase (AKT) signal transduction pathway in protection of propofol on spinal cord ischemic reperfusion injury (SCIRI) in rabbit model before and after ischemia. Methods Sixty Japanese male rabbits were randomly divided into 3 groups (n=20), namely shamoperation group (Group S), ischemiareperfusion group (Group I/R) and ischemiareperfusion group with propofol treatment (Group P).Abdominal aorta of the rabbits in group I/R and group P were blocked by clamp for 40 min and then the clamp was removed.Propofol (30 mg.kg-1) was intravenously infused 10 min before blocking the aorta and at the time of reperfusion.Normal saline was intravenously infused at the same time points in the other two groups.Four rabbits of each group were randomly executed 1, 2, 3, 5, 7 days after surgery.Spinal cord tissues at L3-L4 levles were harvested.Bioactivity of SOD1 was detected by ELISA and mRNA expression levels of SOD1, PI3K and AKT were detected by RTPCR.Results On the 1st day after the surgery, the bioactivity of SOD1 increased significantly in Group I/R and Group P as compared with that in Group S (P<0.05).On the 2nd day, compared with Group S, the bioactivity of SOD1 increased significantly in Group P (P<0.05), but there was no change in Group I/R (P>0.05).On the 3rd, the 5th and the 7th day, compared with Group S, the bioactivity of SOD1 decreased significantly in Group I/R (P<0.05), but there was no change in Group P (P>0.05).Linear regression analysis indicated that there was a positive correlation between the changes of SOD1 activity and the mRNA expression of SOD1, PI3K and AKT respectively in spinal cord tissues.Conclusion Pre and postischemic conditioning with propofol shows potent protective effects against SCIRI in the rabbit model.The mechanisms may be related to increased expression of SOD1 in the spinal cord tissues by activating PI3K/AKT signal transduction pathway.
  • 药物研究
    WANG Xiao-li;ZHOU Jun;LI Mao-xing;QIU Jian-guo;JIA Zheng-ping;ZHANG Ru-xue
    2014, 33(10): 1278-1283. https://doi.org/10.3870/yydb.2014.10.006
    Download PDF ( )   Knowledge map   Save
    Objective To observe the effect of mifepristone (MIF) on the level of corticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH), corticosterone (CORT), insulin (INS) and aldosterone (ALD) in plasma and expression of glucocorticoid receptor (GR) mRNA in hippocampus in type 2 diabetic rats and to discuss the effect and mechanism by which mifepristone improves hyperglycemia. Methods Type 2 diabetes mellitus model was induced by highfat diet plus intragastric administration of low dose streptozotocin (30 mg.kg-1).Rats were randomly divided into normal control group, model control group, positive control (MET) (metformin hydrochloride 200 mg.kg-1.d-1) group, mifepristone low dose (MIF-L) (10 mg.kg-1.d-1), medium dose (MIF-M) (25 mg.kg-1.d-1) and high dose (MIF-H) (50 mg.kg-1.d-1) groups.The normal control group and model control group were given distilled water.Fasting blood glucose (FBG) was measured once a week.The rats were decapitated after five weeks.Organ index, corticotropin release hormone(CRH), adrenocorticotropic hormone(ACTH), corticosterone(CORT), insulin(INS) and aldosterone(ALD)levels were measured.The expression of GR mRNA in hippocampus was measured by using realtime PCR.Results Compared with the normal control group, body weight was decreased significantly (P<0.01), FBG was increased significantly (P<0.01), organ index was increased significantly (P<0.05), CRH, ACTH, CORT, INS and ALD were increased and the expression of GR mRNA in hippocampus was decreased (P<0.01) in the model control group.Compared with model control group, body weight increased in MIF-M and MIF-H groups after administration for 14 days (P<0.01).FBG was decreased in MIF-M group 1 to 4 weeks after administration, with significant difference (P<0.05) at 4th week.The kidney index was decreased in MIF-M and MIF-H groups (P<0.01, P<0.05).CRH, ACTH and CORT were increased, ALD level was decreased in MIF-L group, CRH, ACTH, CORT and ALD were decreased, INS level was increased in MIF-M and MIF-H groups, without statistically significant differences (P>0.05).Relative expression of GR mRNA was significantly increased in MIF-L, MIF-M and MIF-H groups (all P<0.01).Conclusion Hyperglycemia in type 2 diabetic rats can be improved by MIF.The possible mechanism may be related to regulating the HPA axis through inhibiting GR.
  • 药物研究
    CHEN Jing;WANG Wen-qing;SHI Chun-yang;HOU Xiao-long;WAN Jin;FANG Jian-guo
    2014, 33(10): 1283-1288. https://doi.org/10.3870/yydb.2014.10.007
    Download PDF ( )   Knowledge map   Save
    Objective To explore the effects of volatile oil and 2-undecanone from Houttuynia Cordata Thunb.(H.cordata) on LPS-TLR4/MD-2-TNF-α signaling pathway. Methods TLR4/MD-2 blocking agent was used to mask the TLR4/MD-2 site, then protein expression levels of TLR4 in cells treated with volatile oil and 2-undecanone were analyzed by western blot.ELISA was used to detect the secretion of the inflammatory cytokines such as TNF-α, IL-1β and IL-10.Comparison analysis was then performed from the Results of cell experiments in vitro and antiinflammatory effects through xyleneinduced ear edema test in vivo.Results In concentrations between 1 to 10 μg.mL-1,Houttuynia volatile oil showed better effect than 2-undecanone on inhibition of TLR4 protein in LPSinduced RAW264.7 cells, and had some differences in the effects on inflammatory factors. Compared with the LPS+TLR4/MD-2 group, the LPS+TLR4/MD-2 + volatile oil group had no significant difference in the expression of TLR4 protein (P>0.05), but the LPS+TLR4/MD-2 +2undecanone group reduced the expression of TLR4 protein obviously.It appeared that volatile oil exerts its antiinflammatory effect through LPS-TLR4/MD-2-TNF-α pathway, but 2-undecanone may exert its antiinflammatory effect by other means.Houttuynia volatile oil showed better antiinflammatory activity than 2-undecanone in vivo at the same dose.Conclusion There are some differences in antiinflammatory effects and related mechanisms between volatile oil and 2-undecanone, probably owing to the synergistic effects of multiingredients in the volatile oil.
  • 药物研究
    ZHANG Meng-yi;ZHENG Heng;CHEN Ying;CHEN Jian-hua
    2014, 33(10): 1288-1290. https://doi.org/10.3870/yydb.2014.10.008
    Download PDF ( )   Knowledge map   Save
    Objective In vitro detection of antiproliferative effects of P161 combined with cisplatin (DDP) on multiple cancer cells. Methods Growth inhibition rates of HepG2, HT29, IE8, Panc-1 and MA-782 treated by different concentrations of DDP, P161 and P161 combined with DDP were determined by MTT assay.Results DDP and P161 dosedependently inhibited proliferation of multiple tumor cells.A synergistic effect was found in DDP combined with P161 and there was a significant difference in the effect between DDP combined with P161 and DDP alone (P<0.05).DDP dose could be decreased to reach the same inhibitory effect.In the same concentration gradient of DDP combined with P161, the inhibition rate of Panc-1 was low and that of MA-782 was high.Conclusion P161 can increase the sensitivity of tumor cells to DDP.The combination of P161 and DDP can reduce the effective therapeutic concentration of DDP.
  • 药物研究
    SHEN Fang-li;HUO Shi-xia
    2014, 33(10): 1291-1293. https://doi.org/10.3870/yydb.2014.10.009
    Download PDF ( )   Knowledge map   Save
    Objective To study the effects of the gingerol on the melanogenesis in melanoma B16 cells. Methods Melanoma cells were cultured with gingerol at 12.5,25.0,50.0,100.0,200.0 μmol.L-1 and positive control drug hydroquinone, respectively, using Dulbecco’s modified eagle’s medium(DMEM) as the blank control group. The cell proliferation was measured by methyl thiazolyltet tetrazolium(MTT) colorimetric assay.The tyrosinase activity and melanin content were measured by colorimetry assay.Results Gingerol at different concentrations had inhibitory effect on B16 cell proliferation compared with the blank control group(P<0.05 or P<0.01), the inhibition rate being more than 48%at the dosage of 200.0 μmol.L-1.Tyrosinase activity was inhibited significantly compared with blank control group(P<0.05 or P<0.01), the inhibition rate being up to 50%at the dosage of 200 μmol.L-1.Melanin content was also decreased at all levels of gingerol compared with blank control group(P<0.05 or P<0.01), but not in a dosedependent manner.The inhibition rate of melanin content reached the plateau at gingerol levels greater than 25.0 μmol.L-1.Conclusion Gingerol can inhibit the cell proliferation, tyrosinase activity and decrease melanin synthesis in certain range of concentrations.
  • 药物研究
    HAN Chao;ZHENG Lin-ying;LYU Jun-hua;ZHAO Ru-xia;ZHOU Yong-biao;PAN Wei-song
    2014, 33(10): 1294-1299. https://doi.org/10.3870/yydb.2014.10.010
    Download PDF ( )   Knowledge map   Save
    Objective To investigate the effect of total glucosides of paeong (TGP) on the liver lipid infiltration and fibrosis in rats with nonalcoholic fatty liver disease (NAFLD) induced by fructose and highfat diet. Methods Fructosehighfatty induced NAFLD rat model was established.Metformin (MET, 200 mg.kg-1) and TGP (200, 100 mg.kg-1) was intragastrically given to the rats in the treatment group, TGP high dose and low dose group, respectively.Normal control group and model control group was intragastrically treated with equivalent distilled water (10 mL.kg-1).At the fourth week after the treatment, all the rats were sacrificed and the indices such as serum fasting blood glucose(FBG), INS, insulin sensitivity index(ISI), triglycerides(TG), apelin36, visfatin, alanine aminotransferase(ALT), aspartate aminotransferase(AST), free fatty acid(FFA), collagen Ⅲ(COLⅢ), collagen Ⅳ(COLⅣ) were determined.Hepatic content of TG was determined and the pathological changes in the liver tissues were observed under the microscope.Results As compared with the model control group, TGP effectively decreased FBG, INS, TG in serum and liver tissues, activity of ALT and AST in serum and content of FAA, Apelin, Visfatin, COLⅢ and COLⅣ, with significant differences (P<0.05 or P<0.01).TGP alleviated lipid infiltration and fibrosis in rat liver tissues.Conclusion TGP can inhibit effectively lipid infiltration and fibrosis of NAFLD rats, probably through improving glucolipid metabolism and antogonizing insulin resistance.
  • 药物研究
    YANG Bo;ZHOU Cheng-zhi;ZHANG Dao-liang;HU You-zhi;XIAO Jin-feng
    2014, 33(10): 1310-1313. https://doi.org/10.3870/yydb.2014.10.014
    Download PDF ( )   Knowledge map   Save
    Objective To explore the effects of wogonin on hyperlipidemia in mice and clarify the molecule mechanism. Methods Thirty mice were evenly divided into three groups: normal control group, model control group and treatment group.The normal control group was given normal diet, the model control group received highfat diet, the treatment group received highfat diet with wogonin (500 mg.kg-1).Results The mice developed hyperlipidemia 12 weeks after starting the highfat diet.The body weight, visceral fat and fat index were increased (P<0.05).After treatment, these indices were reduced (P<0.01).Wogonin significantly reduced the total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL), except the triglyceride (TG).Compared to the model control group, the hepatic lipase(HL) and lipoprotein lipase(LPL) activity in the treatment group were recovered (P<0.05), but HMG-CoA reductase activity was inhibited (P<0.01).Mechanistic study suggested that the lipidlowering effect might be related to the lipid synthesis genes (SREBP-1c, FAS, PPARγ) and the lipid metabolism genes (PPARα, CPT-1).Conclusion Wogonin can prevent hyperlipidemia, which might be related to the regulation of enzyme activity and the changes of lipid synthesis and oxidative metabolism.
  • 药物研究
    HE Zheng;ZHENG Jun
    2014, 33(10): 1314-1318. https://doi.org/10.3870/yydb.2014.10.015
    Download PDF ( )   Knowledge map   Save
    Objective To study the effects of protein kinase C (PKC) inhibitor staurosporine (STS) on the proliferation and apoptosis of human cholangiocarcinoma QBC-939 cells and to explore its possible mechanism. Methods CCK8 was used to detect the effects of PKC inhibitor STS on the proliferation of human cholangiocarcinoma QBC-939 cells.The effects of STS on the ultrastructural characteristics of QBC-939 cells were observed by routine transmission electron microscopy (TEM).The apoptosis rate and the cell cycle distribution of QBC-939 cells were detected by flow cytometry.The expression of cyclin B1, Cdk1 and pCdk1 in QBC-939 cells was detected by Western blotting.Results STS could significantly inhibit the proliferation of QBC-939 cells in a dosedependent manner (P<0.05) and the half inhibitory concentration (IC50) of QBC-939 cells at 24th and 48th h was 334 nmol.L-1 and 118 nmol.L-1, respectively.TEM observed that STS could induce typical apoptotic bodies and supermicrostructural changes of QBC-939 cells.By Annexin VFITC/PI double labeling flow cytometry, we found that the apoptotic rate of QBC-939 cells after treatment with STS for 0, 12, 24 and 48 h was (10.16±4.52)%, (22.35±2.19)%, (34.27±2.30)%and (59.70±5.97)%, respectively.By flow cytometry, compared with the control group, STS could significantly increase apoptosis rate of QBC-939 cells, decrease the percentage of cells in G0/G1 phase and increase the percentage of cells in G2/M phase (P<0.05).Western blotting proved that the expression levels of cyclin B1 and Cdk1 proteins in the STStreated QBC-939 cells were significantly decreased (P<0.05), while the expression level of p-Cdk1 protein in the STStreated QBC-939 cells was significantly increased (P<0.05).Conclusion STS can significantly inhibit cell proliferation and induce apoptosis of human cholangiocarcinoma QBC-939 cells and the mechanism may be related to cell cycle arrest at G2/M phase.
  • 血液系统疾病专栏
  • 血液系统疾病专栏
    ZHAO Ning;HUANG Yong-ji;MA Guang-bin;YAN Dong-xue
    2014, 33(10): 1321-1325. https://doi.org/10.3870/yydb.2014.10.017
    Download PDF ( )   Knowledge map   Save
    Objective To investigate the effect of ellagic acid extracted from gallnut on multiple myeloma SP2/0 cell line and related mechanisms. Methods Multiple myeloma SP2/0 cell line was treated for 48 h with different concentrations of ellagic acid in vitro.Cell morphology, proliferation, apoptosis and cell cycle were analyzed with microscope, MTT experiment and flow cytometry, respectively.Tumor cell proliferation and apoptosisrelated gene expression of COX-2 were detected by Western blotting.Results Cell cycle was arrested at the G1 phase 48 h after treatment with ellagic acid, the cell in G1 were (55.21±3.01)%,(64.48±0.43)%,(75.10±2.46)%,respectively, with significant difference as compared with control group [(34.04±1.74)%,P<0.01].Cell suppression rate (21.18±5.92)%, (44.58±3.43)%and (70.15±2.90)%, respectively, in 20, 40 and 60 μg.mL-1 ellagic acid treatment groups.Compared with the control group, the differences were significant (P<0.01).Cell apoptosis rate was (9.60±0.56)%, (19.30±1.51)%and (35.10±5.26)%, respectively, in 20, 40 and 60 μg.mL-1 ellagic acid treatment groups, with significant differences as compared with the control group[(3.23±0.85)%, P<0.01].With the increase of drug concentration, COX2 expression was decreased.Conclusion Ellagic acid can inhibit myeloma SP2/0 cell proliferation and promote apoptosis.
  • 血液系统疾病专栏
    WANG Yang;ZHANG Hua-nian;CHEN Yu-jun;XU Hua;LIU Mao-chang
    2014, 33(10): 1327-1331. https://doi.org/10.3870/yydb.2014.10.019
    Download PDF ( )   Knowledge map   Save
    Objective To recheck the reliability of methotrexate (MTX) serum concentration at 48 h (C48 h) in predicting the pharmacokinetic characteristics and toxic reactions at terminal elimination phase after high dose MTX infusion and to provide a reference for determination of rational rescue regimen in clinic practice. Methods In total, 114 cases of children with acute lymphoblastic leukemia (ALL) received 176 courses of high dose MTX chemotherapy treatment.The regimen was continuous infusion of MTX[3-5 g.(m2)-1] in 24 h.Plasma samples were treated with solid phase extraction and serum concentrations of MTX were determined by HPLC at 24, 48 and 72 h (C24 h, C48 h and C72 h) after starting MTX infusion.All data were divided into C48 h≥1 μmol.L-1 group and C48 h<1 μmol.L-1 group.The pharmacokinetic parameters of the two groups at elimination phase were estimated by residual method and the toxic reactions after MTX infusion of two groups were compared by Ridit analysis.Results The C72 h and AUC48were significantly higher in C48 h≥1 μmol.L-1 group than in C48 h<1 μmol.L-1 group (P<0.01).The MTX toxicities to the blood, digestive and hepatic systems were significantly higher in C48 h≥1 μmol.L-1 group than in C48 h<1 μmol.L-1 group (P<0.05).Conclusion C48 h can predict the pharmacokinetic characteristics and toxic reactions at ther terminal elimination phase.Therefore, C48 h≥1 μmol.L-1 can be used as a marker of MTX elimination delay event to guide later rescue regimen.
  • 药物制剂与药品质量控制
  • 药物制剂与药品质量控制
    DING Wei-ming;LI Gui-ling;CAI Cong;WANG Ju-xian;YANG Xin-yi
    2014, 33(10): 1357-1360. https://doi.org/10.3870/yydb.2014.10.028
    Download PDF ( )   Knowledge map   Save
    Objective To determine the solubility and apparent oil/water partition coefficient of sitafloxacin in different solvents. Methods High performance liquid chromatography (HPLC) was used.The column was Dikma Diamonsil C18(2) (4.6 mm×250 mm, 5 μm).The mobile phase was 0.05 mol.L-1 KH2PO4 solution (pH was adjusted with H3PO4 to 2.4)-acetonitrile (70:30).The column temperature was set at room temperature.The flow rate was 1.0 mL.min-1.The detection wavelength was 295 nm and the injection volume was 10 μL.The solubility of sitafloxacin and the apparent oil/water partition coefficient at pH 2.0, 4.3, 5.8, 6.6, 7.4, 8.0, 10.0 and 11.2 were determined.Results The equilibrium solubility of sitafloxacin in water was 0.44 mg.mL-1 and the apparent oil/water partition coefficient was 0.23 (lgP=-0.64) at (37±2) ℃.Sitafloxacin has the lowest equilibrium solubility (0.13 mg.mL-1) and the highest apparent oil/water partition coefficient in pH7.4 buffer solution system.At pH>10 and pH<5.8, the solubility of sitafloxacin increased obviously and apparent oil/water partition coefficient decreased.Conclusion Sitafloxacin is insoluble in water and also poorly soluble in oil, but its solubility could be improved significantly in acidic or alkaline solution.
  • 药物制剂与药品质量控制
    LIU Wei;ZHOU Jian-liang;CHEN Bi-lian;ZHU Ming
    2014, 33(10): 1360-1364. https://doi.org/10.3870/yydb.2014.10.029
    Download PDF ( )   Knowledge map   Save
    Objective To establish the high performance liquid chromatography(HPLC) fingerprint of wuzi yanzong pills. Methods HPLC was performed on Agilent Extend C18column (250 mm×4.6 mm, 5 μm) with a gradient elution system using acetonitrile:methanol (10:1)0.4% phosphoric acid as the mobile phase.The column temperature was set at 30 ℃ and the flow rate was 1.0 mL.min-1.The eluate was detected at the wavelength of 254 nm.Chromatographic peaks were identified by LC-MS method.Results Nine common peaks in wuzi yanzong pill samples were identified by comparing their LC-MS data with those of reference compounds and related reference reports.The HPLC fingerprint of wuzi yanzong pills was finally developed based on the analysis of sixteen batches of samples and their similarities were above 0.93.Conclusion This method has high precision, stability and repeatability.This study could be used for overall quality assessment of wuzi yanzong pills.
  • 药物制剂与药品质量控制
    ZHAO Yan-na;YANG Li;HAO Chun-ying;LI Yan-hong;WANG Xiang-tao;GUO Yi-fei
    2014, 33(10): 1365-1369. https://doi.org/10.3870/yydb.2014.10.030
    Download PDF ( )   Knowledge map   Save
    Objective To investigate the influence of ionic strength on the stability of the methotrexate-loaded dendrimer nanoparticles. Methods The influences of different ions (Na+, Cl-) and different concentrations of sodium chloride on the stability of the nanoparticles were studied.The particle size was measured by dynamic light scattering(DLS) and drugloading content was determined by highperformance liquid chromatography (HPLC) in order to evaluate the stability.Results The Cl- was finally verified to play an important role in stabilizing the nanoparticles and the effective concentration of the sodium chloride was recommended to be below 1.80%. Conclusion The recommended concentration (less than 1.80%) of the sodium chloride significantly improves the stability of the nanoparticles and benefits for long term storage.
  • 药物制剂与药品质量控制
    HE Dan;YANG Lin;ZHANG Jing-qing
    2014, 33(10): 1370-1372. https://doi.org/10.3870/yydb.2014.10.031
    Download PDF ( )   Knowledge map   Save
    Objective To establish a UPLC method for detecting content of imperatorin and isoimperatorin in huoxiang zhengqi oral liquid. Methods ACQUITY UPLC BEH C18 (2.1 mm×50 mm, 1.7 μm) was used and the mobile phase was methanol and water by gradient elution mode.The column temperature was 30 ℃, the flow rate was 0.3 mL.min-1 and the detection wavelength was 248 nm.Results The linear range of imperatorin was 1.305-13.050 μg.mL-1 and the regression equation was as follows: Y =13 633X+3 976 (r=0.999 9).The linear range of isoimperatorin was 0.596-5.960 μg.mL-1 and the regression equation was Y=10 661X+1 073 (r=0.999 9).The average recovery was 99.25%(RSD=0.74%) and 98.94%(RSD=0.63%), respectively.Conclusion The method is accurate, rapid and reliable, and can be used to determine imperatorin and isoimperatorin in huoxiang zhengqi oral liquid.
  • 药物制剂与药品质量控制
    DENG Yin-hua;ZHANG Wei
    2014, 33(10): 1373-1375. https://doi.org/10.3870/yydb.2014.10.032
    Download PDF ( )   Knowledge map   Save
    Objective To establish a method for the quantitative determination of sildenafil in aphrodisiacs by liquid chromatographymass spectrometry (LC-MS). Methods Chromatography was carried on a Waters BEH C18 column (50 mm×2.1 mm, 1.7 μm) with the column temperature being 35 ℃ and 0.02 mol.L-1 ammonium acetate water solution (containing 0.1% acetic acid)-acetonitril (70:30) as the mobile phase at a flow rate of 0.2 mL.min-1.Determination was performed by multiple reaction monitoring mode in two channels, 475→100, 475→283, using electrospray ionization in positive ion mode.Results Sildenafil had a good linear relationship in the range of 0.1986-1.986 ng with the linear regression equation being Y=27 750X+15 232 (r=0.995 2).The average recovery was 96.6% with RSD being 1.8%(n=9).The limits of quantitation and detection were 0.04 ng and 0.01 ng, respectively.Conclusion The determination Results of 10 batches of samples show that the method is sensitive and accurate and can be used as an appropriate technique to detect sildenafil in aphrodisiacs.