肿瘤科用药专栏
HUANG Hui;JIANG Hongling;LIU Fenju;BAI Xue;HE Zhuokai;CAI Rui;ZHANG Rongjun;ZHAO Bo;LIU Meilian
2014, 33(1): 34-38.
ABSTRACT Objective To investigate the antiproliferation effects of arsenic trioxide(As2O3)on human glioma cells44(SHG44) in vitro and its mechanism.MethodsThe inhibitory ratio of the cells by As2O3 was measured by MTT assay.The intracellular ROS were determined by a probe ( DCFHDA) under a laser scanning confocal microscopy.ResultsAs2O3 significantly inhibited SHG44 cells proliferation.The proliferation inhibition rate was dosedependent and timedependent.The proliferation inhibition by 12 μmol8226;L-1 As2O3 on SHG44 cells was as high as 63.8%.After treatment with As2O3, the intracellular ROS was increased markedly in a concentrationdependent manner.ConclusionAs2O3 can inhibit the proliferation of SHG44 cells in both dosedependent and timedependent manners.The inhibition was associated with elevation of intracellular ROS .