中国科技论文统计源期刊　中文核心期刊  
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    药物研究
  • 药物研究
    Comparative Evaluation of Anti-radiation Effect of Pterostilibene and Resveratrol
    BAI Jia-li;YU Cheng-cheng;WANG De-zhi;HAO Jian-xiu;SHEN Xiu;WANG Hao;LIU Xiao-qiu;LONG Wei;ZHOU Ze-wei
    2013, 32(4): 415-418. https://doi.org/10.3870/yydb.2013.04.001
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    Objective To compare the anti-radiation effect between pterostilibene and resveratrol in equimolar oral doses on ICR mice. Methods ICR mice of 6–8 weeks were selected to receive whole body irradiation of 6.5 Gy γ-ray.The mice were randomly divided into six groups to receive the following treatment:Control group, 0.05% CMC-Na; positive control group, rubidate 100 mg.kg-1 ; low- and highdose resveratrol groups, resveratrol at 50 or 150 mg.kg-1 ; low- and high-dose pterostilibene groups, pterostilibene at 56 or 168 mg.kg-1.The mice of the above groups were administered intragastrically 3 days, 2 days, and 1 day before the radiation.After the radiation, the mice were administered intragastrically for 5 consecutives days.The thymus, liver, spleen, lung and bilateral femurs were weighed to calculate corresponding organ indexes.White blood cell count (WBC), blood platelet (PLT), bone marrow nucleated cells (BMNC) and bone marrow DNA were determined, so as to evaluate the radiationprotective effect of pterostilibene and resveratrol. Results Compared with control mice, the indicators of all experiment groups were increased and most have significant difference (P<0.05); the antiradiation effect of pterostilibene was better than resveratrol at the same equimolar oral dose, with significant difference between the lowdose pterostilibene (56 mg.kg-1 ) group and the lowdose resveratrol (50 mg.kg-1 ) group. Conclusion Pterostilibene has better antiradiation effect on ICR mice than resveratrol, and the antiradiation effect of pterostilibene at 56 mg.kg-1 is the best.
  • 药物研究
    Effect of Tetramethylpyrazine on Rat Vascular Calcification and the Related Mechanism
    SUN Na
    2013, 32(4): 418-423. https://doi.org/10.3870/yydb.2013.04.002
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    Objective To investigate the effect and possible mechanism of tetramethylpyrazine involved in regulating vascular calcification. Methods Angiosteosis in rats was induced by vitamin D3 intramuscular injection and nicotine intragastric administration.Calcification of vascular smooth muscle cells (VSMC) was induced by β-glycerophosphate.The Ca2+ contents in aorta and VSMCs were measured by an atom absorptive spectrophotography.Ca2+ deposition in aorta and VSMCs was evaluated by the 45CaCl2 absorption and Von Kossa staining.The alkaline phosphatase (ALP) activity was detected by a chemical colorimetric method.The mRNA level of osteopontin (OPN) was determined by reverse transcription PCR. Results ALT activity, Ca2+ contents and 45Ca2+ deposition were significantly increased, but osteopontin mRNA expression was significantly decreased in calcified vessel and VSMC.After treatment with tetramethylpyrazine (40 mg.kg-1 in vivo and 200 mg.L-1 in vitro), the Ca2+ content, 45Ca2+deposition and ALP activity were decreased, while osteopontin mRNA expression was increased in plasma, aorta and VSMCs, respectively. Conclusion Our Results indicated that tetramethylpyrazine significantly protects the aorta against calcification partly through the activation of the endogenous anticalcification system in local aorta tissues.
  • 药物研究
    Effect of Serum Containing Banshi Culuan Zhuyun Soup on Proliferation of Ovarian Granulosa Cells of Human
    HE Guo-zhen;CEN Yan-hui;YANG Mei-chun;LI Mi;LI Li;WU Cheng
    2013, 32(4): 424-427. https://doi.org/10.3870/yydb.2013.04.003
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    Objective To observe the effect of serum containing banshi culuan zhuyun soup on proliferation of human ovarian granulsa cells. Methods According to the serum pharmacological method, serum containing high, medium and low doses of banshi culuan zhuyun soup was prepared.The human ovarian granulosa cells were collected from the patients and incubated for 24 h.The serum containing three different doses of banshi culuan zhuyun soup and normal serum were added to the cell culture system.MTT assay was used to detect the cell proliferation at 0, 12, 24, 36, 48, 60, and 72 h. Results MTT assay showed that the serum containing banshi culuan zhuyun soup did not have significant effect on cell proliferation before 24 h (P>0.05).But at 36, 48, and 72 h, it showed that the A values of the different experimental groups were significantly higher than that of the control group (P<0.01).Compared with the low dose group, A values of the medium and high dose groups were significantly different (P<0.01).There was no significant difference in A values between the high dose and medium dose groups (P>0.05). Conclusion Serum containing banshi culuan zhuyun soup can effectively promote proliferation of the ovarian granulosa cells in a dosedependent manner.
  • 药物研究
    Relationship of Anti-oxidative Effect of Taxane and Apoptosis
    DUAN Rui-sheng;ZHANG Hui-ping;XIE Tao;LI Yan;LI Li-tao;DONG Mei;WANG Wei-ping
    2013, 32(4): 428-431. https://doi.org/10.3870/yydb.2013.04.004
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    Objective To study the antioxidative effect of taxane. Methods Oxidative SK-N-SH cell model was established by treatment with suitable concentration of hydrogen peroxide.7-Deacetyl-taxine B was extracted and purified from Taxus cuspidate, and used to treat the oxidative SK-N-SH cells.Cell viability was evaluated by using 3-(4,5)-dimethylthiahiazo (-z-y1)3,5-di-phenytetrazoliumromide (MTT) assay.Apoptosis rates were detected using flow cytometry and the ultrastructure was observed by electron microscopy.Expression of Caspase-3 was analyzed using Western blotting. Results The viability of SK-N-SH cells was detected with the ratio of (99.32±4.30)% (P>0.05) after treated with 100 μmol.L-1 7-Deacetyl-taxine B, and the viability of oxidative SK-N-SH cell model after treated with 1, 10 and 100 μmol.L-1 7-Deacetyl-taxine B were (25.90±1.61)%, (31.20±1.25)%, (57.6±3.74)% (P<0.05) ,respectively.After AnnexinV/PI staining the livability rate and apoptosis rate in negative control group were (50.23±5.31)% and (23.27±1.62)%, and the rates were (71.20±3.20)%, (19.20±2.11)%(P<0.05) after treated with EGCG, and they were (79.95±6.13)%(P<0.05), (13.09±1.8)%(P<0.05) after treated with 100 μmol.L-1 7-Deacetyl-taxine B.In negative control group, cells showed features of apoptosis, but after treated with 7-Deacetyl-taxine B the apoptosis and ultramicrostructure destruction were obviously alleviated.The caspase3 protein expression of negative control group obviously decreased from (1.30±0.16) to (0.77±0.20) (P<0.05) after treated with7-Deacetyl-taxine B. Conclusion The anti-oxidative activity of 7-Deacetyl-taxine B can decrease apoptosis.
  • 药物研究
    Determination of Ziprasidone in Human Serum by RP-HPLC
    WANG Ze-min
    2013, 32(4): 432-434. https://doi.org/10.3870/yydb.2013.04.005
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    Objective To develop a high performance liquid chromatography (HPLC) method for the determination of ziprasidone in human serum. Methods The serum samples were extracted with ether and separated on an Zorbax Eclipse XDB C18 column using methanol-0.03 mol.L-1 ammonium acetate (70:30) as the mobile phase.The flow rate was 0.8 mL.min-1.The fractions were detected by diode array detector (DAD) with wavelength at 254 nm. Results The linear range of ziprasidone was 10-1 000 ng.mL-1.The correlation coefficient was 0.991 7 and the limit of quantitation (LOQ) was 10 ng.mL-1.The matrix effect was from 92.1% to 106.7%.The extraction recovery was in the range of 72.3%–80.4% (n＝5).The intra-day and inter-day precision (RSD) were 4.7%-6.4% (n＝5) and 8.9%-12.1% (n＝5), respectively. Conclusion The HPLC method is simple, sensitive and accurate.It is suitable for the determination of ziprasidone in human serum.
  • 药物研究
    Effect of Analgecine on mRNA Expression of Bcl-2 in Perienchyma of Cerebral Contusion Focus
    HU Jin;ZHANG Xiao-li;ZHANG Hui
    2013, 32(4): 435-437. https://doi.org/10.3870/yydb.2013.04.006
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    Objective To investigate mRNA expression of Bcl-2 in perienchyma of cerebral contusion focus and effect of analgecine on it. Methods The cerebral contusion rat model was established by Feeney’s falling body injury and treated with analgecine.The brain was taken and sectioned 24 and 72 h after the treatment.Bcl-2 mRNA positive cells were determined by using in situ hybridization and medical image analysis technique. Results The number of Bcl-2 mRNA positive cells and the average gray value of the treatment group were significantly higher than those of sham operated group and model group (P<0.05).Those were significantly higher in the 72 h group than in the 24 h group (P<0.05). Conclusion Analgecine could protect the neurons from cerebral contusion.It can promote expression of Bcl-2 mRNA and alleviate neuron injury in penumbra area.
    • 肿瘤科用药专栏
  • 肿瘤科用药专栏
    c-Myc Regulates Proliferation of HT-29 Cells Through HIF-1α
    CHEN Cheng;WANG Gui-hua;YANG Xi;LI Xiao-lan;TAO De-ding;GONG Jian-ping;HU Jun-bo
    2013, 32(4): 438-442. https://doi.org/10.3870/yydb.2013.04.007
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    Objective To investigate the influence of c-Myc on hypoxiainducible factor 1, α subunit (HIF-1α) and proliferationof HT-29 cells. Methods The plasmid pcDNA3.1-c-Myc was constructed and transfected into human colon carcinoma cell line HT-29.Efficiency of transfection was detected by Western blotting.The mRNA and protein levels of HIF-1α in normoxia and hypoxia were measured by realtime PCR and Western blotting.The mRNA levels of vascular endothelial growth factor (VEGF) and eNOS (the target genes of HIF-1α) were measured by realtime PCR.PcDNA3.1-c-Myc plasmid and HIF-1α siRNA were cotransfected into HT-29 cells.MTT was used to detect the proliferation of the HT-29 cells. Results The plasmid pcDNA3.1-c-Myc was successfully constructed.After transfection into HT-29 cells in normoxia and hypoxia, realtime PCR showed that the mRNA level of HIF-1α had no change, while Western blotting showed the protein level of HIF-1α was significantly increased.The mRNA levels of VEGF and eNOS were significantly increased.The proliferation of HT-29 cells was stimulated through the overexpression of c-Myc.But cotransfection of pcDNA3.1-c-Myc plasmid and HIF-1α siRNA decreased cell proliferation in HT-29 cell line. Conclusion c-Myc regulates the proliferation of HT-29 cells through HIF-1α.
  • 肿瘤科用药专栏
    Over-expression of Hint2 Sensitizes Breast Cancer Cell MCF7 to Taxol
    CAI Shao-xin;CHEN Cheng;YANG Xi;DENG Yu;LI Zhao-ming;LI Xiao-lan;HU Jun-bo
    2013, 32(4): 442-445. https://doi.org/10.3870/yydb.2013.04.008
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    Objective To evaluate the effect of overexpression of Hint2 on sensitivity of breast cancer cell MCF7 to taxol . Methods The expression vector of pCMV-HA-Hint2 was constructed and transfected into MCF7 cells and the protein expression of Hint2 was detected by immunofluorescence and Western blotting.The changes of sensitivity to taxol after transfection were examined by MTT and flow cytometry respectively. Results In MCF7 cells, the protein levels of Hint2 were significantly increased after transfection, After exposed to 5 μmol.L-1 taxol for 24 h, cell viability of Hint2 overexpression group was 33.78%, compared to 44.12% in control group. Conclusion Overexpression of Hint2 can enhance the sensitivity of MCF7 to taxol.
  • 肿瘤科用药专栏
    Effect of Rapamycin Combined with Cisplatin on Apoptosis and Autophagy of Ovarian Cancer Cell Line SKOV3
    XU Guo-cai;ZHANG Xin;XIANG Yuan-yuan;LIN Zhong-qiu;ZHANG Bing-zhong
    2013, 32(4): 446-449. https://doi.org/10.3870/yydb.2013.04.009
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    Objective To study the effect of rapamycin combined with cisplatin on apoptosis and autophagy of ovarian cancer cell line SKOV3, and to elucidate the possible mechanism. Methods Ovarian cancer cell line SKOV3 was treated with rapamycin alone or in combination with cisplatin.Apoptosis rate was measured by flow cytometry (FCM).The expression of mTOR mRNA was detected by qRTPCR, and the expression levels of Beclin1, LC3 and bcl-2 were detected by Western blotting. Results The apoptosis rate of the cells treated with rapamycin combined with cisplatin was (12.520±1.499)%, significantly higher than those of the other groups (P<0.05).The mRNA level of mTOR was decreased significantly in SKOV3 cells treated with rapamycin combined with cisplatin for 24 h (P<0.05).The expression levels of Beclin1 and LC3II were significantly increased in SKOV3 treated with rapamycin combined with cisplatin (P<0.05), while the expression level of bcl-2 was significantly decreased (P<0.05). Conclusion Rapamycin combined with cisplatin could promote the apoptosis and autophagy in ovarian cancer cell line SKOV3 via inhibiting the expression of mTOR.
  • 肿瘤科用药专栏
    Effect of Celecoxib Combined with PDTC on Apoptosis and Expression of KDR and AQP1 in Gastric Cancer MGC803 Cells
    CEN Wei-li
    2013, 32(4): 450-454. https://doi.org/10.3870/yydb.2013.04.010
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    Objective To explore the effect of celecoxib combined with PDTC on apoptosis and expression of KDR and AQP1 in gastric cancer MGC803 cells. Methods The cells were cultured in RPMI1640 and divided into four groups:control group, 25 μmol.L-1 Celecoxib group, 50 μmol.L-1 PDTC group, and 25 μmol.L-1 Celecoxib + 50 μmol.L-1 PDTC group, which were subjected to treatment of drugs for 48 h.The inhibition rate was detected by methyl thiazolyl tetrazolium (MTT), the cell cycle was analyzed by flow cytometry and the invasion ability of MGC803 cells was tested by transwell chamber assay.The mRNA and protein expression levels of KDR and AQP1 were detected by RT-PCR and Western blotting, respectively. Results Celecoxib and PDTC inhibited the growth of MGC803 cells in a dosedependent manner.Combination of them exerted greater growth inhibition effect than either of them (P<0.01).Celecoxib and PDTC both induced apoptosis of MGC803 cells, and their combination produced a higher apoptosis rate (P<0.01).Celecoxib in combination with PDTC also induced G1 phase arrest and downregulated the expression of KDR and AQP1 in MGC803 cells as compared with either Celecoxib or PDTC (P<0.05). Conclusion Celecoxib in combination with PDTC has a synergistic effect to inhibit cell growth and migration, and downregulates the expression of KDR and AQP1 in MGC803 cells.Combination of celecoxib and PDTC is a potential alternative for gastric cancer therapy.
  • 肿瘤科用药专栏
    Palonosetron Hydrochloride Injection in Prevention of Moderate to Severe Chemotherapyinduced Nausea and Vomiting
    ZOU Yan-mei;XIONG Hua;HU Guang-yuan
    2013, 32(4): 455-458. https://doi.org/10.3870/yydb.2013.04.011
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    Objective To assess the advantage of palonosetron hydrochloride injection for preventing chemotherapyinduced nausea and vomiting. Methods The patients were randomized to receive a single dose of palonosetron 0.25 mg (group A, n＝77), or tropisetron 5 mg (group B, n＝78) 30 min before initiation of moderate or severe emetogenic chemotherapy.Acute and delayed vomiting, nausea, and adverse effect etc.in the patients were strictly observed and recorded.The quality of life (QOF) was evaluated. Results Effective rates for controlling acute and delayed vomiting, 0-120 h vomitting were significantly higher in group A (81.8%, 70.1% and 67.5%) than in group B (51.3%, 43.6% and 41.0%).Time for controlling vomitting was (95.07±42.17) h and (62.07±50.13) h in group A and B, respectively (P<0.05).The number of times was (1.46±2.12) per case and (2.96±3.13) per case in group A and B, respectively (P<0.05).The time length from chemotherapy to first rescue medication in group A was not longer than group B (P>0.05).However, the number of times of rescue medication in group A was significantly lower than that of group B (P<0.05).The nausea grade in group A was 0.86±0.69, which was significantly lower than that of group B (1.29±1.03), P<0.05.The FLIE score of group A was significantly higher than that of group B (P<0.05).The adverse events were similar, including headache, constipation and diarrhea. Conclusion Plonosetron hydrochloride injection is superior to tropisetron for preventing chemotherapyinduced vomiting and nausea, especially for delayed vomiting and nausea, and it can improve QOL.
  • 肿瘤科用药专栏
    Clinical Efficacy of Inductive Chemotherapy with Docetaxel Plus Nedaplatin Followed by Concurrent Nedaplatin with Radiotherapy for Locally Advanced Nasopharyngeal Carcinoma
    WU Fang;WANG Ren-sheng;WEI Bo;ZHANG Yong;LIU Wen-qi
    2013, 32(4): 459-463. https://doi.org/10.3870/yydb.2013.04.012
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    Objective To observe the shortterm efficacy and acute adverse effect of inductive chemotherapy with docetaxel plus nedaplatin followed by concurrent treatment of nedaplatin and radiotherapy in patients with locally advanced nasopharyngeal carcinoma (NPC). Methods Sixty patients with locally advanced NPC confirmed by histopathology were randomized into experimental group and control group.The patients received two cycles of inductive chemotherapy with docetaxel plus nedaplatin in experimental group or cisplatin plus 5fIuorouracil in control group every 3 weeks, followed by concurrent radiotherapy and weekly nedaplatin or cisplatin chemotherapy.All the patients received intensity modulated radiotherapy (IMRT) which began at the 7th week.The prescription doses were as follows:GTVnx 68-70 Gy/30 fraction, GTVnd 66-68 Gy/30 fraction, CTVl 60-64 Gy/30 fraction, CTV2 50-54 Gy/30 fraction, 5 fraction/week. Results More chemotherapy cycles were finished in experimental group than in control group (7.67 vs.7.17, t＝2.574, P＝0.013).The volume of GTVnx in experimental group and control group was reduced by 15.44 cm3 and 12.21 cm3 respectively after inductive chemotherapy (P>0.05).The volume of GTVnd in experimental group and control group was reduced by 8.97 cm3 and 6.74 cm3, respectively (P>0.05).There was no significant difference in complete response rate between the two groups (P>0.05).Incidence rate of grade 34 neutropenia was higher in experimental group than in control group (33.3% vs.10.0%, t＝4.812, P<0.05), whereas more patients in control group suffered from nausea and vomiting than those in the experimental group (6.7% vs.30.0%, t＝5.455, P<0.05 and 0% vs.26.7%, t＝7.067, P<0.01, respectively).There was no significant difference in oneyear survival rate between the two groups (P>0.05). Conclusion Treatment with docetaxel plus nedaplatin is effective and welltolerated in patients with locally advanced NPC.The shortterm efficacy between experimental group and control group is similar.Experimental group also shows a significant superiority in reducing nausea and vomiting.
  • 肿瘤科用药专栏
    Determination of Cytotoxicity of Lentinan on Tumor Cells by MTT Assay in vitro
    HU Jing-na
    2013, 32(4): 464-466. https://doi.org/10.3870/yydb.2013.04.013
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    Objective To study the cytotoxicity of lentinan in vitro. Methods Cytotoxicity of lentinan on human prostate cancer cell line PC-3, human lung cancer cell line A-549, human hepatoma cell line HepG2 and human leukemia cell line K-562 was determined by MTT assay. Results The IC50 values of PC-3, A-549, HepG2 and K-562 were (25.8±3.4), (90.0±7.8), (63.3±4.2), and (33.1±3.3) μg.mL-1, respectively.The growth curve showed that inhibitory effect of lentinan at 100 μg.mL-1 on prostate cancer cell line PC-3 and HepG2 was increased over time. Conclusion The lentinan has cytotoxicity in cancer cells in vitro.
    • 药物与临床
  • 药物与临床
    Effects of Dexmedetomidine on Perioperative Pulmonary Shunt Fraction for Patients with Esophageal Cancer
    LIANG Guo-jin;LUO Ai-lin
    2013, 32(4): 469-473. https://doi.org/10.3870/yydb.2013.04.015
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    Objective To investigate the effect of dexmedetomidine on perioperative pulmonary shunt fraction for patients with esophageal cancer. Methods Sixty patients, ASAⅠ or Ⅱ, scheduled for esophagectomy under general anesthesia were randomly assigned into three groups (20 patients in each group) to intravenously receive treatment of high-dose dexmedetomidine (HDex), low-dose dexmedetomidine (LDex) or normal saline.Patients in group HDex and group LDex respectively were administered intravenously at a loading dose of 0.6 μg.kg-1 and 0.3 μg.kg-1 dexmedetomidine over 10 min, respectively.Both group HDex and group LDex were treated with a maintenance dose of 0.3 μg.kg-1.h-1 over half an hour.Patients in placebo group received normal saline.After anesthesia, at 15 min after twolung ventilation (TLV1), 15 min and 1 h after onelung ventilation (OLV1 and OLV2), 15 min after restoration of two lung ventilation (TLV2), 1 and 6 h post operation (P1 and P6), blood from internal jugular vein and radial artery was collected for blood gas analysis.The intrapulmonary shunt (Qs/Qt) was calculated. Results At OLV1 and OLV2, pulmonary shunt fraction in the blank group was significantly different from those of the highdose group and the lowdose group (OLV1:LSD-t respectively equals -3.03 and -3.39, P<0.05; OLV2:LSD-t respectively equals -2.91 and -2.65, P<0.05), but the values between the high-dose group and low-dose group were not significantly different (P>0.05). Conclusion Dexmedetomidine exerts a greater impact on the pulmonary shunt fraction over time of onelung ventilation, however, during twolung ventilation and 6 h post operation, the impact is not significant.
    • 药物制剂与药品质量控制
  • 药物制剂与药品质量控制
    Research on Value Assignment and Uncertainty Evaluation of Purity Reference Material of Amygdalin
    ZHOU Zheng-zheng;GUO Yong-hui;YANG De-zhi;LV Yang
    2013, 32(4): 504-510. https://doi.org/10.3870/yydb.2013.04.030
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    Objective To develop the purity certified reference material of amygdalin which has traceability, value transmission and highaccuracy quantity value, and to establish an accurate definite value analytical method. Methods The purity value of purity certified reference material of amygdalin was determined by combined value assignment of high performance liquid chromatography (HPLC) area normalization method and differential scanning calorimetry (DSC) on the basis of Technical Norm of Primary Reference Material and related documents requirements. Results The developed purity certified reference material of amygdalin was in accordance with Technical Norm of Primary Reference Material requirements and the standard value and uncertainty was (99.3±0.4)% (k＝2, P＝0.95). Conclusion The combined different principles analytical technology method could overcome the defects of one method and guarantee for the accurate value assignment results of the purity reference material of amygdalin in this study and it was an internationally accepted National Certified Reference Material which could provide reference material, material standard and scientific foundation of standard method for the international competition of related medicines.
  • 药物制剂与药品质量控制
    Extraction of Chemical Constituents of Volatile Oil from Pinecone of Pinus thunbergii Parl.by CO2 Supercritical Fluid Extraction and Steam Distillation
    LIANG Jie;SUN Zheng-yi;ZHU Xiao-yong;LIANG Chen-yan
    2013, 32(4): 510-513. https://doi.org/10.3870/yydb.2013.04.031
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    Objective To compare the chemical constituents of volatile oil from the pinecone of Pinus thunbergii Parl.by two extraction methods . Methods The volatile oil was extracted from the pinecone of Pinus thunbergii Parl.by supercritical fluid extraction (SFE) and steam distillation (SD) methods, respectively.The chemical constituents of volatile oil were separated and analyzed by gas chromatographymass spectrometry (GC-MS). Results Fortythree kinds of chemical components of the pinecone of Pinus thunbergii Parl.were identified by SFE and 37 components by SD, which accounted for 60.78% and 86.16% of the total volatile oil, respectively. Conclusion The chemical constituents and their contents of volatile oil extracted by two methods were different significantly, and the extraction yield of SFE method is lower than that of SD method.
  • 药物制剂与药品质量控制
    Preparation and Quality Evaluation of Compound Metronidazole and Chlorhexidine Acetate Suppositories
    GUAN Yan-bin;TIAN Yu-dong
    2013, 32(4): 514-516. https://doi.org/10.3870/yydb.2013.04.032
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    Objective To prepare compound metronidazole and chlorhexidine acetate suppositories and evaluate the quality. Methods Fatty glyceride and camphor were used as the vehicle.Metronidazole and chlorhexidine acetate were used as main ingredient.The quality of the suppositories were determined by high performance liquid chromatography (HPLC) on C18 column (4.6 mm×250 mm, 5 μm) with methanol and 10 mmol.L-1 sodium heptanesulfonate (containing 0.3% triethylamine, pH adjusted to 4.0 by phosphoric acid) (70:30:0.3) as mobile phase.The flow rate was 1 mL.min-1 and detecting wavelength was 260 nm. Results The quality of the suppositories can be evaluated by characteristics, identification, and content assay.The linear range of metronidazole and chlorhexidine acetate was 8.94-107.28 and 0.764 8-8.952 0 μg.mL-1 , and the average recovery was 100.2% (RSD＝0.5%) and 100.2% (RSD＝1.0%), respectively. Conclusion The preparation technique is simple.The determination method is rapid, simple and accurate, and can be used for the quality control of suppository.
  • 药物制剂与药品质量控制
    Determination of Monosaccarides and Dissaccharides in Dendrobium officinale by HPLC-ELSD
    ZHANG Lin-jie;WANG Ru-wei;HE Hou-hong;WU Jian;WANG Jian-fang
    2013, 32(4): 517-520. https://doi.org/10.3870/yydb.2013.04.033
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    Objective To develop a method for determination of monosaccarides and dissaccharides in Dendrobium officinale by HPLCELSD. Methods Shodex Asahipak NH2 (250 mm×4.6 mm, 5 μm) was used as chromatographic column.Mobile phrase was acetonitrile-water (78:22).Flow rate was set at 1.0 mL.min-1.Column temperature was 30 ℃ .ELSD parameters were as follows: temperature of drift tube: 100 ℃, gas flow: 1.60 L.min-1. Results The assay displayed good linearity.The linear ranges were from 5.91 to 36.28 μg (r=0.999 8) for fructose, from 4.87 to 23.31 μg (r=0.999 9) for glucose, and from 5.91 to 36.28 μg (r=0.999 9) for sucrose.The recoveries ranged from 95.20% to 103.0%. Conclusion The method possesses advantages of easy operation, high accuracy and good reproducibility, and is suitable for determination of monosaccarides and dissaccharides in Dendrobium officinale.
  • 药物制剂与药品质量控制
    Preparation and in vitro Study of Coenzyme Q10 Ophthalmic Microemulsion Gel
    DONG Shao-hua;CHEN Ying;SU Wei;HU Xiao;HU Jing-bo
    2013, 32(4): 520-524. https://doi.org/10.3870/yydb.2013.04.034
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    Objective To prepare Coenzyme Q10 microemulsion gel for topical ocular drug delivery. Methods Gel type was selected by observing the flow state of microemulationgel at room temperature.Gel material was chosen according to the viscosity changes of microemulation gel after addition of the artificial tear.The microemulation gel quality was evaluated by studying its physiochemical property, rheological and in vitro release characteristics. Results Ionic gel was chosen as the final formulation material.It could be used with a small quantily and it is liquid at room temperature.After added with artificial tears, its viscosity was rapidly increased.After dispersion, the average particle diameter of the microemulsion gel was 30.41 nm.The in vitro release experiment showed that the preparation could continuously released coenzyme Q10 over 24 h.Rheology found it was aspseudoplastic fluid. Conclusion In this study, the ophthalmic emulsion gel can be sustainedly released, and is suitable for ophthalmic application.
  • 药物制剂与药品质量控制
    Studies on Chromatographic Fingerprints of Pueraria lobata and Pueraria thomsonii
    WANG Jin-hua;YAO Zhong-qing
    2013, 32(4): 525-529. https://doi.org/10.3870/yydb.2013.04.035
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    Objective To establish chromatographic fingerprints for the identification and quality evaluation of Pueraria lobata and Pueraria thomsonii. Methods Seventeen samples of Pueraria thomsonii and 7 samples of Pueraria lobata were analyzed with the thinlayer chromatography (TLC) and the developed high performance liquid chromatography (HPLC) fingerprint method.The HPLC data was calculated with similarity evaluation system for chromatographic fingerprint of TCM (Version 2004A). Results A satisfactory fingerprint was obtained by HPLC method, and qualitative analyses of some of the peaks were carried out by HPLC/MS approach. Conclusion The present research provides useful evidence for the quality control of evaluation of Pueraria lobata and Pueraria thomsonii.
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  • 用药指南
    Continuous Assessment and Analysis of Serum of Valproic Acid
    ZHOU Jin-yu;SUN Zeng-xian;XU Jing
    2013, 32(4): 534-536. https://doi.org/10.3870/yydb.2013.04.038
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    Objective To continuously assess and analyze valproic acid (VPA) via drug monitoring for quality control, to choose the optimal quality control rule which suits our laboratory, and to ensure the quality of drug monitoring. Methods The VPA quality control data were statistically analyzed, by using LeveyJennings method combined with Westgard rules. Results Continuous assessment showed that 8 control Results were out of control.The new quality control rule was 12S/13S/22S/41S/7tr. Conclusion The LeveyJennings method combined with Westgard rules can timely discover the cause of determination errors, increase the accuracy of determination, and is suitable for clinical therapeutic drug monitoring.
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