Archive

• 01 November 2013 Volume 32 Issue 11

    

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  药物研究
  代谢性疾病用药专栏
  药物制剂与药品质量控制
  

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药物研究
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  药物研究

  Antibreast Cancer Cells Activity of Dδ tocotrienols

  FAN Caiwen;WANG Jianhong;TIAN Jing;XIAO Shengjun;PAN Hongjie;LUO Qin;XIANG Qiu

  2013, 32(11): 1395-1398.

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  ObjectiveTo explore the biological activity of Dδ tocotrienols against human breast cancer MCF7 cells.MethodsThe Breast cancer MCF7 cell line was treated in vitro with 0 (control)，30 (low)，40 (middle)，and 50 (high) μmol•L－1 Dδtocotrienols，respectively for 24 h.Cell growth was observed under the microscope.MTT analysis and flow cytometry were applied to measure cell proliferation，cell cycle and apoptosis.ResultsDδtocotrienols inhibited the proliferation of MCF7 cell line.The cell proliferation inhibition rates of the low，middle，and high dose groups were（9.33±1.68）%，（32.47±3.18）%and（76.63±1.00）%，respectively，compared with the control group.The differences were statistically significant (P＜0.01).The percentage of G1 phase cells was（54.34±1.50）%，（58.24±0.81）%and（62.11±1.12）%，respectively in the experimental groups，all significantly higher than（43.34±2.51）%in the control group (P＜0.05).The percentage of adherent cells was significantly reduced，and the floating dead cells significantly increased in the experimental groups，The rates of early apoptosis were significantly increased by（10.87±0.50）%，（17.87±2.40）%，and（25.83±3.76）%，respectively，compared to that by（1.93±0.25）% in the control group (P＜0.05).ConclusionDδtocotrienols has antibreast cancer activity by inhibiting the proliferation of MCF7 cell line，leading to cell cycle arrest at G1 phase and inducing apoptosis.
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  药物研究

  Comparation of Inclusion Efficiency and Antitumor Effect of Glaucocalyxin A Included by Two Kinds of Cyclodextrin

  ZHANG Chong;SHANG Xiaojun;MA Suying;BAI Suping

  2013, 32(11): 1399-1402.

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  ObjectiveTo prepare glaucocalyxin A inclusion complex with two kinds of material and compare their solubility in vitro and antitumor effect in vivo.MethodsThe glaucocalyxin A inclusion complexes were prepared with hydroxypropyβ cyclodextrin(HPβCD) or γcyclodextrin(γCD).They were identified by differential thermal analysis (DTA).UVspectrophotometry method was applied to determine the contents of glaucocalyxin A.The phase solubility，solubility and dissolution rates of the two complexes and their effects on tumor weight of S180 transplantation in mice were compared.ResultsThe phase solubility，solubility and dissolution rates of glaucocalyxin A were enhanced in both kinds of inclusion complex，with γCD being a better inclusion material.Tumor inhibition rate against S180 was 43.87%and 30.66%from γCD and HPβCD included glaucocalyxin A，respectively.ConclusionγCD is superior to HPβCD for preparation of glaucocalyxin A inclusion complex，demonstrating better solubility，dissolution rate and tumor inhibition properties.
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  药物研究

  Radioprotective Activity of 2,2dimethyl Thiazolidine Derivatives

  YU Guangyun;ZHAO Bin;SHEN Xiu;LONG Wei;ZHANG Hao;JIANG Ming;LIU Peixun

  2013, 32(11): 1403-1406.

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  ObjectiveTo investigate the radioprotective effect of four derivatives of 2,2dimethyl thiazolidine in mice.Methods30day survival rate and the immunological，hematopoietic parameters were adopted to explore radioprotective effect of the four derivatives.ResultsCompared with the control group，white blood cells(WBC)，nucleated cells and DNA content in bone marrow(BMNC) of marrow，spleen colony forming unit (CFUS)， thymus and spleen index of mice treated with different dosage of the four derivatives were increased in different degree.Compound 2 and compound 3 increased the survival rate to 58.3% and the mean survival time compared to the controls, compared with the control group, the difference has statistical significance.ConclusionThe four derivatives of dimethyl thiazolidine have a certain protective effect on immune and hematopoietic system，in which compound 2 and compound 3 show a better overall radiation protection.
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  药物研究

  Effect on the Biofilm Formation of Drug Resistant Helicobacter Pylori by Chinese Herbal Extracts

  HUANG Yanqiang;HUANG Ganrong;LI Xiaohua;HUANG Xiaofeng;WEI Liandeng;WEI Hongyu;CHEN Yuanhong;TANG Huaying;YANG Shan;QIN Yanchun

  2013, 32(11): 1407-1409.

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  ObjectiveTo explore the influence of Chinese herbal extracts on biofilm formation of drug resistant Helicobacter pylori.MethodsThe standard agar dilution method was used to determine the MIC of emodin，berberine，matrine，and baicalin to drug resistant Helicobacter pylori，and detect the optical density under concentrations less than MIC.Confocal microscopy was applied to observe biofilm formation of drug resistant Helicobacter pylori.ResultsThe MIC of emodin，berberine，matrine and baicalin on drug resistant Helicobacter pylori were 64，64，128 ，256 μg•mL－1，respectively.Biofilm formation of Helicobacter pylori was significantly less under confocal microscope with the concentrations of emodin，berberine，matrine and baicalin at 32 μg•mL－1，32 μg•mL－1，64 μg•mL－1 and 128 μg•mL－1，respectively.ConclusionEmodin，berberine，matrine and baicalin can inhibit drug resistance Helicobacter pylori，and suppress the formation of bacteria biofilm at the dose of 50%MIC.
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  药物研究

  α-Glucosidase Inhibitory Activity of the Seeds from Cassia obtusifolia L.and Its Processed Products

  ZHANG Wei;PENG Tao;YIN Zhenhua;KANG Wenyi

  2013, 32(11): 1410-1413.

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  ObjectiveTo study the αglucosidase inhibitory activity of extracts from the seeds of Cassia obtusifolia L.and different processed products.MethodsThe αglucosidase inhibitory activity was screened by αglucosidase inhibitory model with acarbose as positive control in vitro.ResultsAll extracts from the seeds of C.obtusifolia and different processed products had certain αglucosidase inhibitory activity，and the activity of other extracts was higher than that of acarbose except MeOH total extracts of salt steamed and fried seeds of C.obtusifolia，and nBuOH extacts of wine fried and crude seeds of C.obtusifolia.The total MeOH extracts of wine steamed，vinegar steamed and wine fired products showed higher inhibitory activity (IC50＝ 147.40，71.28 and 1 064.52 μg•mL－1，respectively) than that of total MeOH extract of the crude (IC50＝1 387.06 μg•mL－1).The petroleum ether extracts of wine steamed，vinegar fired，wine fired and fired products had higher inhibitory activity (IC50＝ 51.27，61.27，136.91 and 61.67 μg•mL－1，respectively) than that of the petroleum ether extract of the crude (IC50＝842.82 μg•mL－1).EtOAC extracts of vinegar fired and wine fired and fired products had higher inhibitory activity (IC50＝ 81.68 and 328.19 μg•mL－1，respectively) than that of the EtOAC extract of the crude (IC50＝413.83 μg•mL－1).The nBuOH extacts of salt steamed，wine steamed，vinegar fired showed higher activity (IC50＝691.55，750.00，585.10 and 1 185.37 μg•mL－1，respectively) than that of nBuOH extact of the crude (I＝40.66%).Petroleum ether extracts of wine steamed，vinegar fired and fired products，and EtOAC extract of vinegar fired product had the strongest inhibitory activity，the IC50 values of which were less than 100 μg•mL－1.The inhibitory activity of extracts was positively correlated with concentrations.ConclusionThe processing methods for preparing C.obtusifolia impacted their αglucosidase inhibitory activity in various degree.
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  药物研究

  Influences of Benazepril and Irbesartan on Kallikrein 1 Expression in Rat Kidneys with Diabetes Nephropathy

  LIU Xinyu;WEN Yujie;LIU Taowen;DENG Lian;CHEN Xin

  2013, 32(11): 1414-1417.

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  ObjectiveTo investigate the effect of reninangiotension system blocking on kallikrein 1 (KLK1) expression in rat kidneys with diabetes nephropathy.MethodsSixty male SD rats were injected with low dosage streptozotocin and fed with high fat and sucrose diets to establish a diabetes nephropathy (DN) model.Then the DN animals were randomly divided into five groups，ten rats in each group，as normal controls (group A)，diabetic nephropathy model (group B)，benazepril treatment (group C)，irbesartan treatment (group D)，benazepril combined irbesartan groups (group E).The rats were sacrificed after 8 weeks.The kidney weight/body weight，24hours proteinuria，serum creatinine and urea nitrogen were measured to evaluate renal function.KLK1 was stained by immunohistochemical (IHC）technique in kidneys.ResultsIt was demonstrated that the expression level of KLK1 in group B，C，D，E were significantly higher than that in group A(P＜0.01).Compared with the group B，the expression of KLK1 in group C，D，E were increased (P＜0.05).Compared with the group C and group D，the KLK1 expression level was elevated in group E (P＜0.05).There was no significant difference between the group C and D (P＞0.05).ConclusionIt is suggested that benazepril and irbesartan may increase the expression of KLK1 in renal tissues of DN rats by blocking RAS.

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  药物研究

  In vitro Antibacterial Activity of Burn Liniment

  ZHAO Qiansi;ZHAO Chaoli;WU Jinhu;CHEN Yonggang;YE Ziqing

  2013, 32(11): 1418-1421.

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  ObjectiveTo investigate the in vitro inhibitory effect of the burns liniment.MethodsThe disc diffusion method was applied to study the antibacterial activity of the burns liniment with original and new formulation against three common pathogens (Staphylococcus aureus，Pseudomonas aeruginosa，Escherichia coli) in burns，with saline and chlorhexidine as a control.The agar dilution method was used to detect the minimum inhibitory concentration (MIC) of the new formulation of the burns liniment and the positive control agent AmPoSa against the selected bacteria.The MIC of the new formulation against isolated species from secretion of clinical burn wounds was also determined.ResultsThe inhibition zone diameter for the new formulation of the burns liniment against Staphylococcus aureus，Pseudomonas aeruginosa，Escherichia coli was（16.13±0.27），（15.83±0.20），（15.55±0.15） mm，respectively，significantly different for the original formulation (P＜0.01).The inhibitory activity against Staphylococcus aureus and Pseudomonas aeruginosa was superior to chlorhexidine (P＜0.05); The MIC to three pathogens of the new formulation of the burns liniment was 1.56,6.25,1.56 mg•mL－1 ,respectively，which showed inhibition against the isolates from secretion of clinical burns.ConclusionThe burns liniment has a good antibacterial effect against Staphylococcus aureus，Pseudomonas aeruginosa，Escherichia coli.
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  药物研究

  Effect of the Extraction of Buckwheat Flower and Leaf on Immunity Function of H22 Tumor Mice

  GUO Lan;WU Aiping;ZHAO Zhiyu;HAN Shuying

  2013, 32(11): 1421-1424.

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  ObjectiveTo investigate the effect of EBFL on the immune function of H22 tumor mice and explore its possible antitumor mechanism.MethodsThe abdominal cavity of mice was inoculated with H22 cells to maked ascites tumor model.The mice were divided into EBFL low dose（0.2 g•kg－1•d－1），EBFL high dose（0.4 g•kg－1•d－1），CTX（0.025 g•kg－1），EBFL high（0.4 g•kg－1•d－1）plus CTX（0.025 g•kg－1），model and control groups.EBFL was administered intragastrically every day for 2 weeks and CTX was given as intraperitoneal injection every other day.Routine blood examination was performed.The thymus gland and spleen were weighed，and thymus gland（spleen）index after administration for 2 weeks was calculated.Phagocyte function was examined by carbon particle clearance method and the cell conversion function of spleen lymphoid cells was observed with MTT method.ResultsEBFL slightly inhibited growth of H22 tumor in mice，but tumor inhibition effect of EBFL was significantly less than that of CTX（P＜0.05）.Phagocytosis index of phagocytes（0.079±0.020）and lymphoid cell conversion rate（0.298±0.033）in EBFL high group were significantly higher than those in model group and CTX group.ConclusionEBFL could alleviate CTXinduced immunosupression by boosting the immune function of H22 tumor mice.
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  药物研究

  Effect of Jiawei Yubai Pills on Immunological Function of Mice

  LIU Xiaomin;LIANG Yi

  2013, 32(11): 1425-1427.

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  ObjectiveTo study the effects of jiawei yubai pills on immunological function of mice.MethodsThe mice were randomly divided into five groups，namely the normal control，the positive control，Jiawei yubai pills treatment groups（lowdose，middledose and highdose）.The clearance rate of carbon particles and serum hemolysin assay were used to test the function of mononuclear phagocytes，the immune organ mass，and the influence of hemolysin antibody in mice.ResultsMononuclear phagocyte count was increased significantly in jiawei yubai pills treated mice.The phagocyte count in the lowdose and highdose groups was significantly higher than that of the normal control（P＜0.05 and P＜0.01，respectively）.The phagocytic activity in the middledose and highdose groups was significantly higher than that in the normal control group（P＜0.05 and P＜0.01，respectively）.Jiawei yubai pills could increase the spleen and thymus indices of the normal mice，without significant difference from the normal group.Jiawei yubai pills increased hemolysins in mice dosedependently（P＜0.01）.ConclusionJiawei Yubai pills can enhance the immunological function of mice.
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  药物研究

  Intervention Effect of Ease Powder on Locus Coeruleus/Noradrenergic System of Rats with Chronic Unpredictable Stress

  DING Xiufang;TAO Yang;CHEN lei;CHEN Xiaomin;LI jing;ZHU Xiaoxia;SUN Xuegang;FAN qin;LV Zhiping

  2013, 32(11): 1428-1431.

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  ObjectiveTo study the abnormal behavior and expression of tyrosine hydroxylas (TH) and dopamineβhydroxylase (DBH) in Locus coeruleus (LC) in rats under chronic unpredictable stress，and evaluate the therapeutic effect of Ease powder.MethodsA total of 24 male SD rats were randomly divided into control，model，and treatment (ease powder，fluoxetine) groups (n＝ 6 in each group).The rat model was established by body tightbinding.The openfield test was performed.The LC was isolated from the brains 22 days post modeling，and the mRNA expression levels of TH and DBH in LC were analyzed by realtime PCR.ResultsCompared with normal control，the squares crossed，rearing and grooming were significantly reduced (P＜0.01)，and the mRNA expression levels of TH and DBH were significantly increased (P＜0.01) in the model group.As compared to the model control，the ease powder and fluoxetinetreated groups exhibited significant increase in the squares crossed，rearing，grooming time and decrease in mRNA expression levels of TH and DBH (P＜0.01) .ConclusionChronic unpredictable stress regulates body’s mood reactionlike excitement，stress and anxiety by activating the LC/NE (Locus coeruleus/noradrenergic) system，leading to catecholamine release and TH and DBH increase in NE neurons.The ease powder can effectively reverse the abnormal behavior and downregulate TH and DBH expression.
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  药物研究

  Simultaneous Determination of Five Antiepileptic Drugs in Human Plasma by HPLC

  YI Lixin;MA Haiying;GONG Xuefei

  2013, 32(11): 1432-1435.

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  ObjectiveTo establish a method for simultaneous determination of 5 antiepileptic drugs in human plasma by high performance liquid chromatography (HPLC).MethodsThe analysis was performed with an Agilent Eclipse XDBC18 analytical column (4.6 mm×150 mm，5 μm) by gradient elution of water and methanol.The flow rate was 1 mL•min－1.The detection wavelengths were set at 210 nm during 0 to 10 min and 230 nm during 10.01 to 15 min.ResultsThe linear ranges of diazepam，clonazepam，carbamazepine，phenytoin sodium and phenobarbital were 0.1－20.0 μg•mL－1，0.01－10.0 μg•mL－1，0.2－40 μg•mL－1，2－40 μg•mL－1 and 2－80 μg•mL－1，respectively.All the calibration curves showed good linearity (r≥0.997 4) in the concentration ranges.The RSD of intra and interday of the 5 analytes was less than 15%，and the recoveries were 74.4%－91.3%.ConclusionThe developed HPLC assay is accurate，sensitive and reliable，and it can be readily utilized as a reference method for rapid clinical determination.
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  药物研究

  Bioequivalence Study of Voriconazole Capsules

  LEI Xiaoguang;LU Hua;HE Chengzhang;JIANG Weizhe

  2013, 32(11): 1435-1438.

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  ObjectiveTo establish an analyltical method for assessing voriconazole in human plasma and study the relative bioavailability and bioequivalence of voriconazole capsule in healthy volunteers.MethodsTwenty healthy male volunteers were given a single oral dose of domestic voriconazole capsule (tested preparation) or imported voriconazole tablet (reference preparation)，respectively，according to a randomized cross over design.The plasma concentrations of voriconazole were determined by LCMS/MS.The pharmacokinetic parameters and relative bioavailability were calculated by DAS 2.0 program for evaluating bioequivalence of two formulations.ResultsAfter a single dose，the main pharmacokinetic parameters of test and reference preparations were as follow：tmax was (1.35±0.66)，(1.38±0.79) h; Cmax was (1 122.91±225.70)，(1 157.13±348.37) μg•L－1; t1/2 was (6.22±4.19)，(6.18±2.71) h; AUC0～24 h was (6 294.59±3 209.73)，(6 310.87±3 150.27) μg•L－1•h; AUC0～∞ was (7 084.89±5 262.36)，(6 918.43±3 993.69) μg•L－1•h，respectively.The relative bioavailability (F0～t and F0～∞) of test formulation was (99.7±12.3) %and (102.4±14.5) %，respectively.No significant difference was found between the main pharmacokinetic parameters of two formulations.ConclusionThe domestic voriconazole capsule and imported voriconazole tablet are bioequivalent.
代谢性疾病用药专栏
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  代谢性疾病用药专栏

  Metaanalysis for Efficacy and Safety of Atorvastatin in Chinese Patients with Hyperlipidemia

  WANG Lijun;LUO Wujin;ZHANG Xinping

  2013, 32(11): 1443-1450.

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  ObjectiveTo evaluate the effectiveness and safety of atorvastatin in the treatment of hyperlipidemia.MethodsThe randomized controlled trials(RCT) on the therapeutic effect of atorvastatin on hyperlipidemia were collected by retrieving CNKI，CBM，VIP and WANGFANG database，and Review Manager 5.0 was applied for Metaanalysis.ResultsTwentythree RCT involving 1 790 cases were included. Atorvastatin showed better hypolipimia effect on the serum total cholesterol(TC)，lowdensity lipoprotein cholesterol(LDLC) and triglyceride (TG) than the controls through Metaanalysis， the differences of which were significant(P＜0.05)［MD=－0.42 ,95%CI(－0.55，－0.28),P＜0.000 01］,［MD= －0.29，95%CI（－0.37，－0.22），P＜0.000 01］and［MD=0.20，95%CI(－0.27,－0.13),P＜0.000 01］.No significant difference between two regimes on the level of creatinekinase(CK)，alanine aminotransferase(ALT)and gastrointestinal adverse reaction was presented(P＞0.05).ConclusionThe current clinical evidences show that atorvastatin is more effective than other statins in lowering TC, LDLC and TG，and has no more adverse reactions.
药物制剂与药品质量控制
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  药物制剂与药品质量控制

  Optimize the SprayDrying Process for Preparing Aqueous Extracts of Orthosiphon Stamineus

  LI Guang;LI Yihang;LI Xuelan;CHEN Xi;

  2013, 32(11): 1474-1477.

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  ObjectiveTo optimize the spraydrying process for preparing aqueous extracts of orthosiphon stamineus .MethodsThe orthogonal method was adopted to investigate three factors including relative density of the extract，inlet temperature and loading speed regarding the shift rate of rosmarinic acid，the moisture and the yield coefficient as indexes on the process.ResultsThe optimized technological parameters were that setting inlet temperature at 120 ℃，taking the extract at relative density of 1.04 g•mL－1，fixing the injection speed at 5 r•min－1.ConclusionThe process is scientific and valid，and the product obtained in this way is stable.Therefore，it can provide scientific basis for commercial process.

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  药物制剂与药品质量控制

  Infrared Fingerprint Study of the Leaf of Dimocarpus longan Lour.from Guangxi

  LIU Xianfu;LIANG Jie;SUN Zhengyi;WENG Ling;LV Songlin

  2013, 32(11): 1477-1479.

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  ObjectiveThis study aimed to establish the infrared（IR）fingerprint for the leaf of Dimocarpus longan Lour.in Guangxi.MethodsThe fingerprint was established by fourier transform infrared spectroscopy（FTIR），and then analyzed by Omic software.ResultsThe IR fingerprint of Dimocarpus longan Lour.was established.There were 5 common peaks common of 10 samples.ConclusionThe specific，rapid and stable method can be applied for quality control of the leaf of Dimocarpus longan Lour..
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  药物制剂与药品质量控制

  Improvement of Identification Method for Fibriuretinin

  PENG Hengyang;XIAO Dan;XU Jing;LIU Tianqiang

  2013, 32(11): 1480-1482.

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  ObjectiveTo improve the identification method for fibriuretinin in Chinese Pharmacopoeia，2010.MethodsThe original thinlayer chromatography（TLC）expansion system of fibriuretinin was adjusted.The influencing factors，repeatability，sensitivity，etc.were studied.ResultsAlcoholethyl acetatemethanolstrong ammonia solution（2.0：1.5：1.5：1.5：0.5）was obtained as a developing agent for identification，because the corresponding spots fibriuretinin were clear and round，Rf was moderate，sensitivity was high，and repeatability was good.At the same time，different sources of silica gel plate and different temperature had no influence on the results.ConclusionThe improved identification method can the effectively used for the quality control of fibriuretinin.
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  药物制剂与药品质量控制

  HPLC Fingerprint of Baisikuaiwa in Wudang Mountain Area

  WANG Xueqin;CHEN Jiyan;LI Cong;ZHAO Zuxing

  2013, 32(11): 1482-1485.

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  ObjectiveTo establish a HPLC fingerprint of Chinese medical herb baisikuaiwa.MethodsAnalysis was carried out at 25 ℃ on a Diamonsil C18（4.6 mm×250 mm，5 μm）eluted with methanol and water in a gradient mode.The flow rate was 1.0 mL•min－1，and the detection wavelength was 232 nm.ResultsSix peaks were identified as the characteristic fingerprints of baisikuaiwa.The similarity of 10 batches of baisikuaiwa was higher than 0.9 in four of the six fingerprint peaks.ConclusionThe method is reliable，repeatable，and can provide a reference for the quality control of baisikuaiwa.
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  药物制剂与药品质量控制

  HPLC Fingerprint of Fushousan Decoction

  JIA Zhiruo;PEI Weijing;WANG Xueyan;ZHEN Hanshen;TANG Xudong

  2013, 32(11): 1486-1490.

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  ObjectiveTo establish the HPLC fingerprint of fushousan decoction.MethodsA HPLC method was established with Atlantis T3 column (4.6 mm×250 mm，5 μm)，using methanolwater0.2%acetic acid as the mobile phase， setting the flow rate at 0.8 mL•min－1 and the detection wavelength at 290 nm.By taking ferulic acid as the reference substance，the fingerprints of 10 batches of fushousan decoction were analyzed under the same chromotographic condition.ResultsThe HPLC fingerprints of fushousan decotion were established，and sixteen common peaks were signed，in which four were originated from Rhizoma chuanxiong and twelve were from both Radix Angelica sinensis and Rhizoma chuanxiong.The similarity threshold of ten batches of samples was between 0.943－0.989.ConclusionThis method is accurate and reproducible，providing a certain reference to effective substances study and quality control of fushousan.
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  药物制剂与药品质量控制

  Preparation and in vitro Release Behavior of Silybin NMeglumine Sustainedrelease Pellet Capsules

  ZHAO Haiyin;ZHAO Yanmin;BAO Qiang;CHEN Feng

  2013, 32(11): 1490-1494.

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  ObjectiveTo prepare silybin Nmeglumine (SLBM) sustainedrelease pellets capsules and study their release in vitro.MethodsThe microcrystalline cellulose (MCC) blankpellets were prepared by means of powder layering with a centrifugal granulation equipment.The yield and size distribution were optimized as the main evaluation index.Then，Eudragit RL 30D/Eudragit RS 30D were used as the coating material to prepare SLBM sustainedrelease pellets.The release properties of coated pellets was studied.ResultsThe optimized formulation and procedure of SLBM sustainedrelease pellets were as follows：taking 1%HPMC(5 cps) as adhesive，setting rotational speed of plate and pulp pump at 130 and 6 r•min－1 ,respectively，atomization pressure as 0.3 MPa，sphronization for 3 min，the layer weight gains of Eudragit RL 30D/Eudragit RS 30D (RS to RL，4：1) being 7%of total weight.The in vitro release of the drug from pellets was in accordance with Firstorder kinetics model.ConclusionThe technology of preparation is reliable and quality controllable，SLBM sustainedrelease pellets prepared in this way show a significant sustainedrelease property.
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  药物制剂与药品质量控制

  Study on the Stability of OCarboxymethyl Chitosan

  WU Di;PU Jinhui;LIU Hui;ZHANG Jing;ZOU Ping;

  2013, 32(11): 1494-1496.

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  ObjectiveTo investigate the stability of selfmade Ocarboxymethyl chitosan(OCMC).MethodsThe rotary viscosimeter was applied to evaluate the property and stability indicated as intrinsic viscosities of OCMC under conditions as various pH，ionic strength，ultraviolet radiation and heat.ResultsThe intrinsic viscosities of OCMC decreased as pH decreased or ionic strength increased，and the intrinsic viscosities decreased as ultraviolet radiation lasted and cultivated longer under 37℃.ConclusionThe pH，ionic strength,ultraviolet radiation and temperature all affect the stability of OCMC.
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  药物制剂与药品质量控制

  Optimization of Extraction Process for Preparing Shenqi Qiangjing Capsule by Orthogonal Design

  TIAN Yuanchun;MA Ruqing;WEI Shuilin;BIN Bin

  2013, 32(11): 1497-1500.

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  ObjectiveTo optimize the extraction process for preparation of shenqi qiangjing capsule.MethodsThe extraction duration，extraction times，and water load were optimized by the L9（34）orthogonal test，using the pasteforming rate，hyperin content and total polysaccharide content as indicators.ResultsThe optimum extraction process was determined by using 12fold volume of water，extracting for 1.5 hours for 3 times.ConclusionThe extraction process is rational，stable and feasible.
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  药物制剂与药品质量控制

  Determination of Residual Solvents in Olsalazine Sodium by GC

  LI Hui;WEI Yingying;FENG Guang

  2013, 32(11): 1500-1502.

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  ObjectiveTo establish a Capillary GC method for determination of the residual solvents in olsalazine Sodium.MethodsDBFFAP capillary column（30 m×0.32 mm，0.25 μm）was used，with the detector as FID.The initial column temperature was kept at 40 ℃ for 4.0 min，then rose to 160 ℃ with an increase rate of 30 ℃•min－1，and sustained for 3 min.Injector temperature was set at 220 ℃ and detector at 250 ℃，respectively.The split ratio was 1：10.ResultsThe calibration curve of residual solvents in olsalazine sodium showed a good linearity.The average recovery was 93.1%－108.2%.ConclusionThe method is specific，accurate，and can be used for the control of the residual solvents in olsalazine sodium.
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  药物制剂与药品质量控制

  Studies on the Specific Chromatogram of Panax Japonicus in Hubei by HPLC

  YANG Tao;CHEN Xin;LI Long;XU Xu;HU Hai;HE Zhou

  2013, 32(11): 1503-1507.

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  ObjectiveTo establish the HPLC fingerprint for Panax japonicus produced in Hubei.MethodsThe analysis was performed on a Apollo C18 column（4.6 mm×250 mm，5 μm），using the mobile phase of acetonitrile0.05% solution phosphoric acid; the detection wavelength was 203 nm; the column temperature was 30 ℃ and the flow rate was 1 mL•min－1.ResultsTen samples collected in different picking time common peaks were confirmed in specific chromatograms.Four compounds were elucidated with the peak of Chikusetsu saponin Ⅴ as reference.The content of 4 major common peaks were 12.616，3.976，5.625，and 4.429 mg•g－1，respectively.ConclusionThe method can be applied to the quality control of Panax Japonicum C.A.Mey.in Hubei.