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    药物研究
  • 药物研究
    TANG Kun;XIE Xiaohui;CHEN Zhida;CHEN Yang;GUO Lianjun
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    ABSTRACTObjectiveTo study the neuroprotective effect of cactus polysaccharides (CP) on ischemia/reperfusion(I/R) injury induced by the middle cerebral artery occlusion (MCAO) in rats.MethodsThe male SD rats were randomly divided into shamoperated,MCAO/R model group,NStreated group,CP- treated group (200 mg·kg-1·d -1,ip).The MCAO/R model was made by MCAO method.The neurological deficits were detected at 3 h,6 h and 8 h after MCAO for 2 h and reperfusion; The cerebral infarct volume was detected by TTC staining 8 h later,the brain tissue sections of MCAO for 2 h and reperfusion for 22 h were stained by hematoxylineosin (H&E) for the morphological observation under microscope.ResultsCompared to the NStreated group,the neurological deficit scores of CPtreated group after reperfusion for 8 h was decreased by (2.35±0.47)(P<0.05),in company with the loss of infarct volume(P<0.05).The rat cortical and hippocampal neuronal cell loss,gliosis,nuclear condensation and nuclear deep dyeing were significantly alleviated (P<0.05). ConclusionThe CP can attenuate of arterial embolism in rats and ameliorate cerebral ischemia,which possessing a neuroprotective effect..
  • 药物研究
    WANG Xibin;LIU Huagang;YANG Bin;HUANG Huixue;LIU Limin
    2012, 31(9): 1113-1116.
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    ABSTRACTObjectiveTo study the effect of the crystal8 from zanthoxylum nitidum(Roxb.) DC.( crystal8) on the expression of cfos gene in rats with nociceptive pain caused by formalin.MethodsA total of 34 female SD rats were randomly divided into 5 groups:the blank control,model control,positive control(rotundine 2 mg·kg-1),high dose of crystal8 groups(2 mg·kg-1),7 rats in each,and 6 rats in the low dose of crystal8 group(1 mg·kg-1) .The expression of cfos gene in painful rats was detected by using immunohistochemistry.ResultsThe positive particles of FOS protein in the brainstem,hypothalamic and spinal cord of the model control group were (20.33±2.63),(19.28±2.42),(18.36±5.11),respectively,those in the high dose of crystal8 group was (10.76±3.66),(12.49±2.80),(6.87±2.10),respectively .ConclusionThe mechanism of analgesia by crystal8 may be related to restraining the FOS protein expression in central nervous tissues.
  • 药物研究
    FANG Ying;DING Fei;WU Lan;LIAO Pengcheng;ZHANG Huihui;LIU Yanwen
    2012, 31(9): 1116-1121.
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    ABSTRACTObjectiveTo comparatively study the chemical constituents of valerian oil which were extracted with different extraction methods.MethodsValerian oil was seperated from valerian by CO2 supercritical fluid extraction or steam distillation extraction.Qualitative study on components and comparison of relative contents were carried out by GCMS.ResultsA total of 118 chemical constituents were identified,98 kinds were obtained and identified via a CO2 supercritical fluid extraction,67 kinds were through steam distillation extraction,among which 47 kinds of constituents were the same.The yield of valerian oil which was extracted by CO2 supercritical fluid extraction was 1.8 times of that by the steam distillation extraction.ConclusionThe results show that CO2 supercritical fluid extraction is more suitable for seperating valerian oil.
  • 药物研究
    SHI Lixia;LIU Xiaomin;ZHANG Zhenjia;ZHANG Dianwen
    2012, 31(9): 1122-1124.
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    ABSTRACTObjectiveTo study the effects of shenshuaikang oral liquid on kidneyyang deficiency mice induced by hydrocortisone.MethodsThe kidneyyang deficiency model was induced by i.p.hydrocortisone in mice.Shenshuaikang oral liquid(30.0,15.0,7.5 g·kg-1)was orally administered once a day for 10 days.Effects of shenshuaikang oral liquid were detected on the body mass,body temperature,immune organs (thymus,spleen,adrenal gland),and genitals (foreskin gland,testis,prostate) in kidneyyang deficiency mice.ResultsThe results showed that body weight,body temperature were improved significantly by shenshuaikang oral liquid at the dose of 30.0 g·kg-1 and the index of thymus,spleen,adrenal gland,prostate,testis and preputial gland were significantly increased.ConclusionShenshuaikang oral liquid can significantly ameliorate kidneyyang deficiency induced by hydrocortisone in mice.
  • 药物研究
    MA Weicheng;XU Airen;DAI Xiaoyan;YING Jingyan;XU Jinlong;HU Jingna
    2012, 31(9): 1125-1127.
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    ABSTRACTObjectiveTo study the toxic effects of the compatibility of aconitum and fritillaria on myocardial cells.MethodsThe in vitro toxicity on myocardial cells of suckling mice with the decoction of Radix Aconiti Kusnezoff or Bulbus Fritillaria thunbergii,and both combined were tested,and the effects of which on lactate dehydrogenase (LDH),Ca2+ and succinodehydrogenase (SDH) were analyzed as well.ResultsThe content of LDH and Ca2+ in the cell transmitter was remarkably elevated at different degrees by the decoction of aconitum or fritillaria and their codecoction,while the activity of SDH was significantly decreased.The toxicity of codecoction was more significant than that of each decoction single.ConclusionThe toxicity is obviously modified with the compatibility of aconitum and fritillaria,which confirming the scientific concept that "aconitum is against with fritillaria".
  • 药物研究
    HUANG Xiaofei;YUAN Sujian;YANG Cheng
    2012, 31(9): 1128-1131. https://doi.org/0.3870/yydb.2012.09.006
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    ABSTRACTObjectiveTo explore the effects of total flavonoids of drynaria on the proliferation and cell cycle of rat dental pulp stem cells.MethodsThe rat dental pulp stem cells(DPSCs) were obtained with a tissue digesting method,which were cloning isolated and cultured for further identification.The dental pulp stem cells were cultured in media with different concentrations of drynaria total flavonoids (0.01 g·L-1,0.05 g·L-1 and 0.10 g·L-1).Then the proliferation was detected by MTT,and the changes of cell cycle in each group were analyzed by flow cytometry.ResultsCD44,CD29 and Stro1 were positive in DPSCs.The proliferation rate of DPSCs treated with the total flavonoids of drynaria was accelerated in comparison to the control group(P<0.05).The proportion of cells in phase S was conspicutously more than that in the control group(P<0.05),and cells at G0/G1 phase were significantly decreased (P<0.05),which were also showed in a dosedependent manner.ConclusionThe total flavonoids of drynaria could promote the proliferation of DPSCs in rats,and the effect of which might rely on expediting DPSCs from phase G0/G1 into phase S.
  • 药物研究
    2012, 31(9): 1132-1135.
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  • 药物研究
    SONG Dan
    2012, 31(9): 1135-1137.
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    ABSTRACTObjectiveTo study the in vitro antibacterial activity of paeonol from cortex moutan.MethodsThe antibacterial activity of paeonol against Escherichia coli,Proteus,Salmonella Typhi,Salmonella paratyphi B,Staphylococcus aureus and Staphylococcus epidermidis was performed by the disk diffusion susceptibility test (KirbyBauer method) and tube double dilution method.ResultsThe bacteriostasis of paeonol against Escherichia coli,Staphylococcus aureus and Staphylococcus epidermidis was the best,with the MIC was 62.5 mg·mL-1.The effects against Salmonella Typhi and Salmonella paratyphi B were inferior to that and the MIC was 125.0 mg·mL-1.The weakest effect was on the Proteus,the MIC of which was 250.0 mg·mL-1 .ConclusionPaeonol shows distinctly antibacterial effect against all of the 6 kinds of bacteria, Staphylococcus aureus ospecially.
  • 麻醉科用药专栏
  • 麻醉科用药专栏
    HE Chao;LUO Ailin;LIU Jiuhong
    2012, 31(9): 1138-1142.
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    ABSTRACTObjectiveTo investigate the effects of dexmedetomidine (Dex) on Narcotrend index (NTI) and propofol requirement of patients undergoing gynecological laparoscopic operation.MethodsForty ASA I/II gynecological patients undergoing laparoscopic operation were randomly assigned into two groups:dexmedetomidine treated group (group D,n=20) and the control group ( group C,n=20).Dex(0.5 μg·kg-1) was diluted into 10 mL for pump intravenous infusion 10 min in group D,followed by 0.4 μg·kg-1·h-1 for maintenance.Saline was applied in the same way in group C.Propofol was used by targetcontrolled infusion (TCI) 15 min after administration,with the target controlled effectsite concentration (Ce) increased gradually at 1.0 μg·mL-1 until the final Ce up to 4.0 μg·mL-1.Propofol and remifentanil were used for the anesthesia maintenance during operation.The vital signs,Ce value,MOAA/S,NTI and propofol requirement were recorded.Results① The NTI in group D was (98.5±0.6),(94.0±4.5),(87.5±5.0),(86.8±7.1) at t1~t3,that in group C was (98.8±0.8),(98.4±0.7),(98.5±0.6),(98.6±0.4),respectively.Compared with group C,the NTI in group D was significantly decreased(P<0.01).②As Ce was set at 1.0,2.0,3.0,4.0(μg·mL-1)during induction,the NTI in group D was(76.4±3.4),(64.5±7.2),(46.8±7.6),(34.3±6.7),which in group C was(83.7±5.4),(69.4±8.1),(52.2±7.5),(38.0±6.6),respectively;and which in group D was significantly lower than that in group C at different Ce values(P<0.05).③Propofol requirement in group D was (9.1±1.2) mg·kg-1·h-1,which was significantly lower than that in group C (11.1±1.5) mg·kg-1·h-1 (P<0.05).ConclusionDexmedetomidine remarkably decreases NTI,enhances the anesthetic effect of propofol and reduces the requirement of propofol without overtly influencing anesthesia recovery.
  • 麻醉科用药专栏
    2012, 31(9): 1142-1144.
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    ABSTRACTObjectiveTo investigate the analgesic effects of parecoxib sodium on transurethral resection of bladder tumor.MethodsA total of 100 patients undergoing selective transurethral resection of bladder tumor under epidural anesthesia were randomly divided into two groups with 50 cases in each.Group P received perecoxib sodium 40 mg i.v.just before the end of operation,and group C received equal volume of normal saline.The pain scores of both groups were assessed by visual analog scale (VAS) within 1 h,3 h,6 h and 12 h postoperation.The incidence of bladder spasm,gastrointestinal side effects and degree of satisfaction in patients were also observed and recorded at the same time point.ResultsThe VAS scores in group P within 12 postoperation were 1.92±0.85,2.35±0.72,2.56±0.91,2.79±1.01 ,respectively,which were significantly lower than those in the control group (P<0.05).The bladder spasm developed in the treatment and control groups within 12 h postoperation was 3.2±1.3,7.8±2.9,respectively.Degree of satisfaction in the former group was remarkably higher than that in the latter.There was no significant difference between both groups.ConclusionIntravenous injection of 40 mg parecoxib sodium exhibits favorable postoperative analgesia and perfect satisfaction,and reduces the bladder spasm in patients undergoing transurethral resection of bladder tumor.
  • 药物与临床
  • 药物与临床
    LI Liang;YANG Jianhong;DAI Xiongkai;SU Wei
    2012, 31(9): 1150-1153.
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    ABSTRACT Objective To investigate the efficacy and safety of paliperidone in treating acute schizophrenia.MethodsEighty patients with acute schizophrenia were randomly assigned to the test group (paliperidone,n=40) and control group (ziprasidone,n=40),who were administrated with paliperidone and ziprasidone,respectively,and were studied for 8 weeks.The clinical efficacy was evaluated with the Positive and Negative Syndrome Scale (PANSS) before and 1,2,4,8 weeks after treatment.Side effects were assessed with the Treatment Emergent Symptom Scale (TESS).ResultsThe total score of PANSS and each point in the two groups were notably decreased (P<0.01) after treatment compared with that before treated.The total scores of PANSS and positive symptoms in the treatment group were significantly better than those in the control by the end of the 1st and 2nd weekends (P<0.05).The effective rate of the two groups was 67.5% and 65.0 %,respectively (P>0.05).The serum prolactin level in the test group was increased significantly after treatment (P<0.01), while which exhibited no significant change in the control group and there was significant difference between two groups.The incidence of abnormal liver function in treatment group was obviously lower than that in the control group (P<0.05).There were no severity adverse effects in both groups.ConclusionPaliperidone shows reliable clinical efficacy,quick effect and safety for treating acute schizophrenia.
  • 药物与临床
    CHENG Aibin;SHANG Xiujuan;DIAO Zengli;WANG Ruigang
    2012, 31(9): 1154-1156.
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    ABSTRACTObjectiveTo study the clinical effects of carbachol on human leukocyte antigen DR (HLADR) in CD+14 monocytes from patients with severe sepsis.MethodsA total of 100 cases of patients with severe sepsis were divided into two groups including the treatment group with 60 and control group with 40.The treatment group was intramuscularly administrated with carbachol at the dose of 0.2 mg·kg-1 for 7 days on the basis of conventional therapy.While the control group was given with placebo at the same time based on routine treatment.Then the proportion of HLADR in CD+14 monocytes and CD+4,CD+8 T cell subsets were investigated before and after treatment.ResultsThe levels of HLADR in CD+14 monocytes and CD+4,CD+8 T cells were significantly increased by carbachol,which were (36.30±13.14)%,(40.81±12.09)%,(31.14±3.63)%,respectively.ConclusionCarbachol can regulate inflammatory reaction status of the body and benefit for the immune function of patients with severe sepsis.
  • 药物与临床
    GU Jian;LI Yansong;GU Jiaowei;LI Jingfeng
    2012, 31(9): 1156-1158.
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    ABSTRACTObjectiveTo study the effects of levocarnitine on the serum levels of TNFα and SOD in children with viral myocarditis.MethodsSixty children with viral myocarditis were randomly divided into two groups with 30 cases in each.The treatment group was administrated with levocarnitine at the dose of 50-100 mg·kg-1·d-1 by intranvenous drip.The control group(n=30) was injected with CoA (100 U·d-1) +ATP at the dose of 40 mg·d-1 in the same manner.The serum levels of TNFα and SOD in patients were detected by RIA before and after treatment .ResultsThe serum level of TNFα in the control group and treatment one before treatment was ( 629.2 ± 30.8) ng·L-1 and ( 628.6 ± 30.4) ng·L-1,respectively,while that of TNFα after being treated was (604.6±28.9) ng·L-1 and (346.5±19.7) ng·L-1,respectively (P<0.01).The pretreatment and posttreatment serum level of SOD was (74.9±7.6) U·mL-1 and (72.5±6.8) U·mL-1,(78.3±7.8)U·mL-1 and (150.3±13.1) U·mL-1,respectively(P<0.01).ConclusionLevocarnitine can reduce the serum levels of TNFα and increase SOD,which exerting a good therapeutic effect on viral myocarditis.
  • 药物制剂与药品质量控制
  • 药物制剂与药品质量控制
    TU Honghai;JIA Zhengping;SHENG Jie;ZHANG Quanlong;LAN Jianguo;LI Ying
    2012, 31(9): 1190-1195.
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    ABSTRACTObjectiveTo study the optimal preparation process of Sankang capsules.MethodsThe optimum extraction process of shengxue capsules was determined by the orthogonal design with pasteforming rate and contents of the special ingredients.The preparation process was optimized through the selection of formulation,materials,wetting agents and conditions for granlunation,and investigation of flowability of particles, hygroscopicity,relative humidity and bulk density of the granules.ResultsThe best extraction technology was as follows:the ethanol extraction was carried out twice,with 10 times volume of 70% ethanol for 2.0 h circumfluence reflux and 8 times of it for 1.5 h,respectively.The water extracted parts were soaked with some water for 1.0 h,refluxed with 12 times volume of water first for 1.5 h and 10 times water for 1.0 h later.The preparation technology was that mixed the ethanol extract with 1.13 relative density,water extract with 1.35 relative density and powder at a certain proportion,vacuumly dried,ground and passed through 80 mesh sieve,and formed softmaterials by using 90% ethanol as wetting agent,then crushed through 20 mesh sieve and loaded into No.0 capsules.The bulk density was 0.659 9 g·(cm3)-1 and the critical relative humidity was 44.2%.ConclusionThe preparation process is reasonable,stable and practicable.
  • 药物制剂与药品质量控制
    LI Maoxing;WEI Lili;QIU Jianguo;ZHANG Quanlong;Qiu Yinong
    2012, 31(9): 1195-1199.
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    ABSTRACTObjectiveTo study the optimal extraction process through orthogonal test and evaluate the antioxidant activities of total flavoniods in Oxytropis falcata Bunge in vitro.MethodsThe optimum extraction process was established by the orthogonal design method,with extraction efficiency and pasteforming rate as assessment standards.The antioxidant activities of the total flavonoids extract were evaluated by Fenton assay,DPPH assay and reduction capability in vitro.ResultsThe optimum extraction process was conducting thermal reflux 1 h at 80 ℃ for three times with 10 times volume of water.The total flavonoids of Oxytropis falcata Bunge extracts was (72.92±5.04) mg·mL-1.The flavonoids extract of Oxytropis falcata Bunge strongly eliminated hydroxyl radical,DPPH free radical,with EC50 as 1.10 mg·mL-1 and 262.57 μg·mL-1,respectively.ConclusionThis process significantly increases total flavoniods in extracts and the method is reliable and simple.The flavonoids extract exhibits a strong antioxidant activity in vitro in a dosedependent manner.
  • 药物制剂与药品质量控制
    NI Yiting;GUO Meihua;WANG Junde;LIU Yun;XIN Yi;WU Dachang
    2012, 31(9): 1200-1202.
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    ABSTRACTObjectiveTo identify qualitatively and analyze the activity of scorpion venom in Buthus Martensii Karsch from different regions by twodimensional gel electrophoresis (2DE),and investigate the protein composition and function differences of scorpion venom.MethodsQuantitive identification and content determination of proteins in scorpion venom were performed following preconditions including dissolution,desalting and condensing of the freezedrying scorpion venom powder.The proteins in scorpion venom were separated by pH gradient isoelectric focusing and SDSPAGE gel electrophoresis.The twodimensional electrophoresis gel map was captured via gel imaging system after silver staining.The special different proteins were determined and comparatively analyzed through PD Quest image analysis software,and thus the scorpion venom from different regions was qualitatively identified.ResultsProtein fingerprints were acquired from three samples.Total protein spots were 80,69,77,and the distinctive number of which were 56,46,55,successively.ConclusionScorpion venom in Buthus Martensii Karsch from different regions separated with 2DE show obviously diverse protein distributions.
  • 药物制剂与药品质量控制
    ZHAO Xiaoyan;LI Li
    2012, 31(9): 1203-1205.
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    ABSTRACT ObjectiveTo establish a HPLC method for determination of candesartan cilexetil and amlodipine in their combination tablets.MethodsA DIONEX Acclaim 120 C18(250 mm×4.6 mm,5 μm)column was used,with acetonitrile 0.05 mol·L-1 KH2PO4(55:45) as a mobile phase.The flow rate was 1.0 mL·min-1 and detection wavelength was set at 254 nm.ResultsCandesartan cilexetil and amlodipine had a good linear relationship wthin 19.4-51.8 and 12.5-33.3 μg·mL-1(r≥0.999 7),and the recoveries were 99.1% and 99.3%,respectively.ConclusionThe method is simple,quick,accurate and suitable for determination of candesartan cilexetil and amlodipine in their combination tablets.
  • 药物制剂与药品质量控制
    XIANG Yanying;JIN Xiaodong;ZHANG Yan;WANG Teng;ZHANG Mengjie;ZHANG Changgong
    2012, 31(9): 1205-1207.
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    ABSTRACT ObjectiveTo establish a method for determination of related substances of ursolic acid raw materials and provide the evidence for its quality control.MethodsThe chromatographic column was Agilent XDBC18(250 mm×4.6 mm,5 μm).The mobile phase consisted of methanolwater phase [water:triethylamine:glacial acetic acid (10:0.03:0.06)].The gradient elution was applied as a chromatographic separation,and the detection wavelength was set at 210 nm. ResultsThe oleanolic acid contained in the ursolic acid was less than 0.5%,and other impurities were not detected.ConclusionThis method is simple,rapid,accurate and has a good specificity that can be used in checking the related substances in raw materials of ursolic acid.
  • 药物制剂与药品质量控制
    LIN Shuyu;LI Yutang;YANG Changyun;HUANG Yang;GAN Huizhen
    2012, 31(9): 1210-1213.
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    ABSTRACTObjectiveTo investigate the appropriate HPLC detection for studying the stability of esomeprazole(Eso) sodium for injection with six kinds of common solvents,and thus provide an evidence for using reasonable compound preparation in clinical use.MethodsThe Eso sodium for injection was compatible with 100 mL six kinds of common solvents respectively, and the pH value was detected.The content of Eso sodium in the compatible solution was analyzed by HPLC.The color and clarity were observed at regular time.ResultsEso showed a good linear relationship within the range of 0.05-1.60 mg·mL-1(R2=0.999 9).There were no significant changes on pH value,color,clarity and content within 24 h after being mixed with 0.9% sodium chloride injection and xylitol one.No distinct changes appeared 12 h,8 h and 6 h after being mixed with 10%GS,5%GS, 5%GNS injection,and the Eso solutions of which were relatively stable.While,the situation was different for Eso mixed with fructose injection.ConclusionEso is incompatible with fructose injection and shows better compatibility with 0.9% NS or 5% xylitol injection.The drip of admixture should be finished within12 h,8 h and 6 h when mixing with 10%GS,5%GS,5%GNS,respectively.