Archive

• 01 August 2012 Volume 31 Issue 8

    

  ---

  药物研究
  药物与临床
  药物制剂与药品质量控制
  

• Select all
  |

药物研究
• Select
  药物研究

  Thermodynamic Effect of the Ternary Complexes of Praseodymium with Salicylic Acid and 8hydroxy Quinoline on Proliferation of HL60 Cells

  ZHANG Hui;LI Juan;RUAN Lijun;YAO Feihong;HU Jilin;LI Qiangguo;

  2012, 31(8): 969-971.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo investigate the thermodynamic effect of the ternary complexes of praseodymium with salicylic acid and 8hydroxy quinoline［Pr（C7H5O3）2（C4H6NO2S）•2H2O］ on the proliferation of HL60 cells in vitro.MethodsMicrocalorimetry was performed to determine the curve of cell proliferation and metabolic heat after the coordination compound was added. Thermodynamic parameters ［growth and metabolism rate constant （κ）, maximum heat output power (Pmax), peak time (tmax) and cell growth inhibition rate (I)］ were analyzed. ResultsThe proliferation activity of HL60 cells was inhibited in a dosedependent manner when treated with different concentrations of ［Pr（C7H5O3）2（C4H6NO2S）］•2H2O. When the cells were treated with 4.0 μg•mL－1 ［Pr（C7H5O3）2（C4H6NO2S）］•2H2O, the κ and Pmax decreased significantly, and the I significantly increased. When the cells were treated with ＞4.0 μg•mL－1 ［Pr（C7H5O3）2（C4H6NO2S）］•2H2O, apoptotic cells were seen. Conclusion［Pr（C7H5O3）2（C4H6NO2S）］•2H2O can affect the growth and metabolism of HL60 cells in a dosedependent manner, and its effective concentration is 4.0 μg•mL－1.
• Select
  药物研究

  Influence of Fuke Qianjin Tablets on ICAM1 and MMP2 in Acute Pelvic Inflammatory Models of Rats

  SHI Zhenyu;GUO Jiansheng;YUAN Jianling;ZUO Zhiwen;YUE Zenghui

  2012, 31(8): 972-974.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo investigate the effects of fuke qianjin tablets on ICAM1 and MMP2 in acute pelvic inflammatory models of rats.MethodsThe rat models with acute pelvic inflammatory disease (Wistar female rats) were successfully established by injecting three kinds of bacteria (Escherichia coli, Ureaplasma urealyticum and Staphylococcus aureus) into the uterus. The rats were randomly divided into 8 groups and the ICAM1 and MMP2 positive cells in the pelvic tissues were tested by immunohistochemistry (SABC), after 21 days of treatment. ResultsCompared with the blank group and sham group, ICAM1 positive cells of model group were significantly increased while MMP2 positive cells were significantly decreased in the pelvic tissues. Compared with the model group, ICAM1 positive cells of fuke qianjin tablets treatment groups were significantly decreased while MMP2 positive cells were significantly increased in the pelvic tissues. ConclusionFuke qianjin tablets can intensely influence the expression of ICAM1 and MMP2 in the pelvic tissues.
• Select
  药物研究

  Effects of Probucol on AGEs-induced eNOS Uncoupling and NADPH Oxidative Stress Pathway

  CHENG Yongxia;GUO Sufen;LIU Guibo;FENG Yukuan;YAN Bin;WANG Hongwei;YU Jianbo;CAO Yong;LI Zhiqiang;JIN Zaishun;CHEN Lili;YANG Xianghong

  2012, 31(8): 975-979.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo observe the effect of probucol on AGEsinduced eNOS uncoupling in cardiac microvascular endothelial cells, and to investigate its mechanism. MethodsThe cardiac microvascular endothelial cells were cultured in vitro. The cells were added with probucol (5, 10 and 20 μmol•L－1, respectively) and AGEs (100 mg•L－1), and incubated for 24 h. Meanwhile, a group managed with serumfree culture solution was assumed as blank control. We determined BH4, NO, O2 generation, eNOS, ROS and p47phox protein expression of all groups. ResultsAs probucol concentration was increased, production of NO was increased gradually (P＜0.05), production of O2 decreased gradually (P＜0.05), BH4 expression increased (P＜0.05), eNOS expression gradually decreased (P＜0.05), ROS was seen decreased gradually (P＜0.05).ConclusionProbucol can inhibit intracellular eNOS uncoupling in the cardiac microvascular endothelial cells, and its mechanism might be related to suppressing the activation of NADPH oxidative stress pathway.
• Select
  药物研究

  Effects of Ginkgo Leaf Extract and Diphyridamole on Apoptosis and TGFβ Activity of Human Peritoneal Mesothelial Cells

  LI Ming;YIN Limin

  2012, 31(8): 979-982.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo study the effect of ginkgo leaf extract and diphyridamole on the proliferation and TGFβ activity of human peritoneal mesothelial cells (HPMCs). MethodsHPMCs were treated with ginkgo leaf extract and diphyridamole injection for 48 h and observed under a microscope by using Hoechst 33258 staining. The cell proliferation was detected with WST8 method. Apoptosis peaks were examined by flow cytometry. TGFβ activity was detected by ELISA. ResultsTreatment of 10 and 100 μmol•L－1 ginkgo leaf extract and diphyridamole could inhibit the proliferation of HPMCs, induce HPMC apoptosis and downregulate the TGFβ activity (before the treatment: 242.20±0.13, after the treatment: 88.78±0.14, P＜0.05). ConclusionGinkgo leaf extract and diphyridamole at 10 and 100 μmol•L－1 induces apoptosis of HPMCs, which is dependent on TGFβ pathway in the HPMCs
• Select
  药物研究

  Experimental Study on Antiinflammatory and Analgesic Effects of Different Extracts from Chinese Lobelia

  HUANG Lide;GUO Liqiang;PAN Tingqi;PAN Xiying;YAN Zudi;LIU Shengli;ZHAO Shanmin;HE Xianjiao

  2012, 31(8): 982-985.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo study analgesic and antiinflammatory effects of different extracts from Chinese lobelia. MethodsWater extract and 75% alcohol extract were obtained from Chinese lobelia. Antiinflammatory effect of Chinese lobelia was studied by using mouse abdominal cavity capillary permeability increase test induced by acetic acid, mouse auricle swelling test induced by xylene, mouse toe swelling test induced by 10% albumen test in mice. Analgesic action of Chinese lobelia was studied by mouse aceticacidinduced twisting test and hot plate test.ResultsIn the same dose, the water extract of Chinese lobelia significantly inhibited the twisting effect induced by acetic acid in the mice (P＜0.01). The extracts of Chinese lobelia increased the threshold of pain in the mice significantly by hot plate test, after the treatment for 1 h or 2 h (P＜0.05, P＜0.01). The extracts of Chinese lobelia inhibited xyleneinduced mouse auricle swelling (P＜0.05). The extracts of Chinese lobelia also significantly inhibited 10% albumeninduced mouse toe swelling, 0.5 h, 1 h, 2 h or 4 h after the induced inflammation (P＜0.05, P＜0.01). ConclusionChinese lobelia has obvious analgesic and antiinflammatory effects.
• Select
  药物研究

  Study on Nimesulideinduced Apoptosis of Human Nasopharyngeal Carcinoma Cells

  JIN Jing;YUAN Yulin;ZHOU Xuhong;ZENG Fandian

  2012, 31(8): 985-990.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo observe apoptosis of nasopharyngeal carcinoma CNE2 cells induced by selective cyclooxygenase 2 (COX2) inhibitor nimesulide (NIM). MethodsThe CNE2 cells cultured in vitro were treated with gradient concentrations of NIM (0, 0.05, 0.1, 0.2, 0.4 and 0.8 mmol•L－1). Light microscope was used to observe morphology & number of the cells. Transmission electron microscope was applied to study changes of cell morphology and structure. Absorbance and viability of the cells was detected by MTT assay. Cell cycle distribution and apoptosis was detected by flow cytometry (FCM). COX2 expression was analyzed by Western blot. ResultsAfter the CNE2 cells were treated with NIM for 3 days, cell shrinkage and reduction was observed under the light microscope, and morphological changes, structural damage and death of the cells appeared under the transmission electron microscope. MTT results showed that the higher the concentration of NIM was, the lower the absorbance of the cell suspension was detected under the same reaction time. FCM results showed that a hypodiploid peak (apoptotic peak) appeared before G0/G1 peak in all dose groups of NIM. With gradual increasing of the concentration of NIM, the cells in G0/G1 phase decreased, while the cells in G2/M phase increased, and apoptotic rate increased gradually. Western blot results showed that NIM decreased the protein expression of COX2. These results were related with the concentration of NIM. Statistical analysis showed that the difference was significant (P＜0.05) as compared with the control group and the adjacent group. ConclusionSelective COX2 inhibitor NIM can induce apoptosis of human nasopharyngeal carcinoma CNE2 cells, which is correlated with its inhibition on COX2 in a dosedependent manner.
• Select
  药物研究

  Study on the Determination of Human Plasma Protein Binding Ratio of the Caspase Inhibitor F1013 and Its Influence Factors

  WU Shan;WEI Junxia;WANG Qiongqiong;WU Jianhong;LIU Yanwen;ZENG Fandian

  2012, 31(8): 990-993.

    Knowledge map   Save

  ObjectiveThe ultrafiltration was employed to determine the human plasma protein binding ratio of a novel Caspase inhibitor F1013. The influence factors of this determination were investigated. MethodsThe unbound fractions in plasma were separated with ultrafiltration equipment under different conditions and were determined with high performance liquid chromatography (HPLC). ResultsThe Amicon Ultra0.5 equipment was suitable for researching the plasma protein binding ratio of F1013. The plasma protein binding ratio of F1013 was increased with the increasing of rotation speed and centrifuge time, and it was decreased with prolongated incubation time. ConclusionHuman plasma protein binding ratio of F1013 was influenced by rotation speed, centrifuge time and incubation time. When the ultrafiltration was applied to study the human plasma protein binding ratio of F1013, the best ultrafiltration conditions are that the human plasma spiked with F1013 was incubated in the 37 ℃ water for 1 h, and then put into the Amicon Ultra0.5 equipment, to centrifuge at 14 000 r•min－1 for 15 min. The method has satisfying recovery, precision, and a good feasibility.

• Select
  药物研究

  Efficacy of Fufang Shenyan Pill on Adriamycin Nephrotic Rats

  WANG Yingjuan;PAN Kaili;ZHAO Xiaoli;QIANG Huan

  2012, 31(8): 994-996.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo observe effects of fufang shenyan pill on the changes of IL18, TGFβ1, ET1 and TNFα in adriamycininduced nephrotic rats. MethodsForty rats were evenly divided into five groups: group A, normal control； group B, nephrotic rats treated with water；group C: nephrotic rats treated with traditional Chinese medicine；group D: nephrotic rats treated with glucocorticoid；group E, nephrotic rats receiving combination therapy. The expression of IL18, TGFβ1, ET1 and TNFα was detected in all the groups by ELISA methods. ResultsThe expression of IL18, TGFβ1, ET1 and TNFα was significantly higher in the groups B, C, D and E than in the group A, and their expression was decreased from group B, C, D to group E (in turn). ConclusionThe fufang shenyan pill can regulate the expression of IL18, TGFβ1, ET1 and TNFα in adriamycininduced nephrotic rats, and the regulation effect can be enhanced when combined with glucocorticoid.
• Select
  药物研究

  Protective Effects of AlphaLipoic Acid on Isoproterenolinduced Myocardial Hypertrophy in Rats

  ZHANG Chi;XIA Hao

  2012, 31(8): 996-999.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo investigate the protective effects of alphalipoic acid on isoproterenol (Iso)induced myocardial hypertrophy in rats. MethodsTwentyfour rats were randomly and evenly divided into 3 groups: control group, model group and alphalipoic acid (LA) group. Myocardial hypertrophy model of rats were induced by injection of Iso (1 mg•kg－1, ip) once daily for 15 days. The control group was treated with the same volume of normal saline injection. The LA group according to the above method was injected with the alpha lipoic acid in the abdominal cavity at 100 mg•kg－1•d－1 once daily for 15 days. The heartweight (HW), left ventricular weight (LVW), the ratios of HW/BW and LVW/BW (LVI) were measured. The content of angiotensin (AngII) and the expression of angiotensin type one receptor (AT1R) in the left ventricle were determined by radioimmunoassay and immunochemistry, respectively. Reverse transcriptionpolymerase chain raction (RTPCR) was used to detect the expression of AT1 receptor mRNA of the left ventricular myocardial tissue. Thiobarbituric acid method and xanthineoxidase method were used to determine the contents and activities of malondialdehyde (MDA) and superoxide dismutase (SOD) in the myocardial tissue, respectively. ResultsThe HW/BW, LVI and the content of AngII and the expression of AT1R mRNA in the left ventricle significantly increased in the model group as compared with the control group. The content of MDA in the left ventricle and blood was increased but SOD decreased (P＜0.01). The cardiac indices were decreased significantly, the content of MDA and Ang II, the expression of AT1R and AT1 mRNA decreased in the LA group as compared with the model group, but the content of SOD was increased (P＜0.01). ConclusionOxidative stress is involved in the development of myocardial hypertrophy. αLA is shown to prevent remodeling of Isoinduced myocardial hypertrophy in rats through antioxidation.
药物与临床
• Select
  药物与临床

  Curative Effect of Levetiracctam Monotherapy on Newly Diagnosed Epilepsy Patients

  YANG Chunqing;LI Zhe;LI Pan;LI Wenling;CUI Xuehua;DU Yali

  2012, 31(8): 1017-1020.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo investigate the therapeutic effect of levetiractam (LEV) on electroencephalogram of epileptic patients. MethodsIn this openlabel selfcontrol study, 120 newly diagnosed epileptic patients received LEV monotherapy. The patients were followed up for therapeutic effect, interictal epileptiform activity (IEA) and electroencephalogram (EEG) background activity two months, six months or twelve months after the therapy. ResultsAfter two months, six months, and twelve months LEV therapy, the total effective rates were 89.2%, 82.5% and 77.5%, and IEA was vanished or decreased ＞50% in 48.3%, 50.0% and 52.5% cases, respectively. After six months LEV therapy, the relative power of θ, δ band significantly decreased (P＜0.05), the relative power of α band increased, and that of β band did not change by brain electrical activity mapping. ConclusionLEV monotherapy is effective for newly diagnosed epilepsy with various seizures, and can diminish or even vanish IEA and improve EEG background activity.
• Select
  药物与临床

  Analgesia Efficacy of Parecoxib for Uvulopalatopharyngoplasty Postoperative Patients

  LI Jun;JIN Jing;WU Xianglei

  2012, 31(8): 1020-1022.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo investigate the analgesia efficacy of parecoxib for uvulopalatopharyngoplasty (UPPP) postoperative patients. MethodsFortytwo patients of obstructive sleep apnea hypopnea syndrome (OSAHS) undergoing UPPP were randomly assigned to treatment group and control group (n＝21 of each). The treatment group patients were given a single dose of parecoxib 40 mg before extubation and 40 mg•d－1 during following 3 days. The control group patients were given normal saline as a placebo. Visual analog scale (VAS) was used to evaluate the degree of postoperative pain and the scores at 2 h, 12 h, 24 h, 48 h post operation and the blood coagulation were recorded. ResultsParecoxib showd significantly postoperative analgesia efficacy (P＜0.05) but little impact on blood coagulation. ConclusionParecoxib could be used safely as postoperative analgesia for OSAHS patients.
• Select
  药物与临床

  Effect of Combined Use of αlipoic Acid, Lipo PGE1 and Danhong on Treatment of Diabetic Peripheral Neuropathy

  LIU Chao;DU Wanhong;ZHENG Taolin

  2012, 31(8): 1023-1025.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo observe the effect of combined use of αlipoic acid, Lipo PGE1 and danhong on the treatment of diabetic peripheral neuropathy. MethodsOne hundred patients with diabetic peripheral neuropathy were randomly and evenly divided into treatment group and control group. All the patients were administered oral antidiabetic drug or injected with insulin to control the blood glucose level. Additionally, the treatment group was given combined use of αlipoic acid, Lipo PGE1 and danhong, but the control group received αlipoic acid for treatment. Michigan symptom score, motor nerve conduction velocity (MNCV) and sensory nerve conduction velocity (SNCV) in both groups were compared before and after the treatment. ResultsAfter the treatment for 14 days, the subjective symptoms and signs were significantly improved with a total effective rate of 90.0% in the treatment group versus 64.0% in the control group. Compared with the control group, the total effective rate of treatment group was significantly higher (P＜0.01). NCV of both groups was significantly improved after the treatment, and the NCV of the treatment group was significantly higher than that of the control group (P＜0.01). In the treatment group, Michigan scores of all symptoms after the treatment were lower than those before the treatment (P＜0.05). In the control group, Michigan scores of hypesthesia and numbness after the treatment were lower than that before the treatment, and after the treatment the Michigan scores of prickling and burning of the treatment group were significantly lower than that of the control group (both P＜0.05). After the treatment, MNCV and SNCV of the treatment group were significantly higher than those before the treatment, and significantly higher than those of the control group as well (P＜0.01). ConclusionCombined use of αlipoic acid, Lipo PGE1 and danhong is effective in the treatment of diabetic peripheral neuropathy.
• Select
  药物与临床

  Clinical Efficacy and Safety of Lowdose rhTNFR: Fc Combined with Methotrexate in Treatment of Rheumatoid Arthritis

  HU Qing;ZENG Yuqin;LI Xuefeng;SUN Mingjin;HUANG Chenghu

  2012, 31(8): 1026-1028.

  Download PDF ( )   Knowledge map   Save

  ObjectiveThe aim of this study was to evaluate the efficacy and safety of combination use of lowdose rhTNFR: Fc and methotrexate (MTX) in the patients with active rheumatoid arthritis (RA). MethodsSixtyfive patients with active RA were divided into two groups: Group A (n=35), treated with combined use of hypodermic injection of lowdose rhTNFR: Fc (12.5 mg•W－1) and orally administration of MTX (10 to 15 mg weekly), and group B (n=30), treated with orally administration of MTX (10 to 15 mg weekly). Arthrocele, joint tenderness, the time of early morning stiffness, and the changes of ESR and RF were recorded before the treatment, one month and 24 months after the treatment. Staging by Xray and Disease Active Score 28 (DAS28) were determined before and 24 months after the treatment. Drug adverse reaction was recorded during the treatment. ResultsClinical indicators were significantly improved better in group A than in group B one month after the treatment (P＜0.01). Changes of staging by Xray in group A were significantly less than those in group B (P＜0.05). There was no significant difference in the drug adverse effect between the two groups. ConclusionLowdose rhTNFR: Fc combined with MTX can reduce joint inflammation, improve physical function rapidly, and retard radiological progression of RA. The efficacy and safety is good.
• Select
  药物与临床

  A Clinical Observation of Lentinan Combined with GP Regimen in Patients with Advanced Nonsmall Cell Lung Cancer

  HAN Liang;LI Jinsong;LIU Yong

  2012, 31(8): 1029-1032.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo evaluate the shortterm efficacy and influence of lentinan combined with gemcitabine and cisplatin (GP regimen) on life quality of patients with advanced nonsmall cell lung cancer (NSCLC). MethodsFiftyone cases histologically confirmed Ⅲb and Ⅳ stage of NSCLC were enrolled and allocated to treatment group (n＝25) and control group (n＝26) randomly. The treatment group received lentinan combined with GP regimen: Lentinan 2 mg intravenously guttae (ivgtt) on days 1 and 4, gemcitabine 1 000 mg•（m2）－1 ivgtt on days 1 and 8 combined with cisplatin 25 mg•（m2）－1 ivgtt on days 2 to 4 in a cycle of 21 days; the control group were treated with simple GP regimen: gemcitabine 1 000 mg•（m2）－1 ivgtt on days 1 and 8, cisplatin 25 mg•（m2）－1 ivgtt on days 2 to 4 in a cycle of 21 days. The efficacy and toxicities were evaluated after two cycles.ResultsThe scores of QLQC30 in the treatment group and the control group were both improved after the treatment, but the global score of QLQC30 was significantly higher in the treatment group than in the control group (P＜0.05). The incidence of fatigue was significantly decreased in the treatment group as compared with the control group (P＜0.05). The scores of QLQLC13 (cough, dyspnea and chest pain) in the treatment group and control group were both decreased after the treatment, and the difference was not statistically significant (P＞0.05). Cell immune function didn’t change before and after the chemotherapy in the treatment group, but declined in the control group (P＜0.05). The incidence of leucopenia was significantly higher in the control group than in the treatment group (P＜0.05). The differences of remission rate and the incidence of gastrointestinal disorder were not statistically significant (P＞0.05). ConclusionLentinan combined with GP regimen can effectively keep disease progression under control, comparing with control group, and alleviate adverse reaction induced by chemotherapy and significantly improve the quality of life in patients with advanced NSCLC.
药物制剂与药品质量控制
• Select
  药物制剂与药品质量控制

  Comparative Study on the Dissolution Character between Berberine and 8hydroxy Dihydroberberine

  WEI Shichao;ZHANG Chenyu;XU Lijun

  2012, 31(8): 1053-1055.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo compare the dissolution character of berberine (Ber) and 8hydroxy dihydroberberine (Hdber). MethodsAn high performance liquid chromatography (HPLC) method was established for determination of Ber and Hdber with C18 column. The mobile phase was cyanideswaterlauryl sodium sulfate KH2PO4 (500 mL－500 mL－3.4 g－1.7 g), adjusted to pH 5.4 with phosphoric acid or triethylamine, and the detection wave length was set at 365 nm. A paddle method was used to investigate their dissolution character. ResultsThe dissolution equilibrium time of Ber was 30 min at 37 ℃, while Hdber needed 90 min. When reached the dissolved equilibrium, the concentrations of Ber and Hdber were 45 μmol•L－1 and 0.35 μmol•L－1, respectively. ConclusionThe method is convenient, stable and reliable, and the dissolution rate of Hdber is significantly lower than that of Ber.
• Select
  药物制剂与药品质量控制

  Determination of Solubility of Sesamin by RPHPLC

  ZHANG Tingting;XU Yuan;CHANG Min;WANG Qingwei

  2012, 31(8): 1056-1058.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo establish a reversedphase high performance liquid chromatographic (RPHPLC) method for determination of sesamin. The solubility in different solvents was determined. MethodsInertsil ODS3 (4. 6 mm×150 mm，5 μm) column was used and detection wavelength was set at 286 nm. The flow rate was 1.0 mL•min－1.ResultsThe results showed that sesamin had a good linear relationship when its concentration was between 1.029 μg•mL－1 and 51.450 μg•mL－1. The regression equation was Y= 1 447.594X－2.852 (r = 0.999 9). Solubility values of sesamin in acetidin were higher than those in the other solvents. ConclusionThis method is simple, quick and accurate for the determination of sesamin.
• Select
  药物制剂与药品质量控制

  Determination of Berberine Hydrochloride in Zuning Granules by HPLC

  GUO Junhao;DU Guang;WANG Pingtao

  2012, 31(8): 1058-1059.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo establish a high performance liquid chromatography (HPLC) method for determination of berberine hydrochloride in zuning granules. MethodsThe HPLC method was employed to determine berberine hydrochloride. The separation was achieved on a Dionex C18 column (4.6 mm × 250 mm, 5 μm) at a flow rate of 1.0 mL•min－1. The mobile phase is acetonitrile0.1% H3PO4 (50：50). The detection wavelength was 265 nm, and the column temperature was set at room temperature. ResultsThe linear range of berberine hydrochloride was within 5－50 μg (r=0.999 9). The average recovery was 100.201% and RSD was 0.49%.ConclusionThe method is simple, accurate and repeatable. It is proved to be suitable for the quality control of zuning granules.
• Select
  药物制剂与药品质量控制

  Study on Preparation and Preliminary Stability of Chlorhexidineiodine Solution

  XU Dan;ZOU Qinghe;LIU Hui;WU Longchuan;GUO Miaomiao;PU Jinhui

  2012, 31(8): 1060-1062.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo prepare chlorhexidineiodine solution and to investigate its preliminary stablilty. MethodsHlorhexidineiodine was synthesized. Singlefactor method was used to optimize the prescription of chlorhexidineiodine solution using nonionic surfactants as solubilizer and a small molecule organic solvent as potential solvent. Low temperature experiment and freeze thawing test were designed to detect the stability of the optimized prescription. ResultsChlorhexidineiodine possessed the best dissolubility when such solubilizers as Cremophor EL, ELP, RH40, Solutol 15 combined with the potential solvent ethanol were used to solubilize chlorhexidineiodine. The optimized prescription of 0.5% chlorhexidineiodine solution was as follows: 10% Cremophor RH40 + 1% ethanol. Its cloud point was 100 ℃. No such changes as preparation characteristics, content and cloud point were found in low temperature test and freeze thawing test. ConclusionThe stable chlorhexidineiodine solution can be prepared successfully using the solubilization principle.
• Select
  药物制剂与药品质量控制

  Determination of Chlorogenic Acid, Caffeic Acid and Rutin in Different Medicinal Parts and Growth Stages of Solanum lyratum Thunb.

  SHU Xiang;CHEN Jun;LIN Shihe;XIE Dai;YU Nancai

  2012, 31(8): 1063-1065.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo study contents of chlorogenic acid, caffeic acid and rutin in different medicinal parts and growth stage of Solanum lyratum Thunb. and to provide a theoretical basis for confirming an optimal recovery season. MethodsThe root, stem, leaf and fruit samples were collected from May to November. High performance liquid chromatography (HPLC) method was used. The Angilent Zorbax ODS C18 columm (4.6 mm×250 mm, 5 μm) was used. The detection wavelength was 327 nm. The mobile phase consisted of acetonitrile1% acetum, and the flow rate was 0.6 mL·min－1. The column temperature was set at 25 ℃. ResultsChlorogenic acid in different medicinal parts of Solanum lyratum Thunb. was comparatively abundant, the distribution of chlorogenic acid content met the following rules: fruit＜upper part stem＜root＜under part stem＜leaf. The maximum values of chlorogenic acid at different medicinal parts were as follows: 2.583 mg·g－1 in the root at August, 2.762 mg·g－1 in the under part stem at August, 1.788 mg·g－1 in the upper part stem at september. The content of chlorogenic acid, caffeic acid and rutin in the leaf was comparatively abundant. The maximum value of caffeic acid in the leaf was 0.397 mg·g－1 at August. The maximum values of rutin and chlorogenic acid were 3.777 mg·g－1 and 8.169 mg·g－1 at November, respectively. ConclusionThe contents of chlorogenic acid, caffeic acid and rutin change as Solanum lyratum Thunb. grows and develops. The active constituent content is most abundant in the leafiness Solanum lyratum Thunb. harvested at August or September.
• Select
  药物制剂与药品质量控制

  Determination of Metronidazole in Complex Oral Ulcer Paster by Gas ChromatographyMass Spectrometry

  CHEN Qinhua;ZHANG Jinfeng;LI Peng;Zhu Jun;

  2012, 31(8): 1066-1070.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo develop a method for the determination of metronidazole in complex oral ulcer paster by gas chromatographymass spectrometry in selective ion monitoring. MethodsThe metronidazole was analyzed quantitatively on the DB5 quartz capillary chromatographic column by selective ion monitoring. The sample was extracted in ethanol. ResultsThe linear range of metronidazole was 5－400 μg•mL－1 (r=0.999 4), the average recovery was 96.0% (RSD=2.08%, n=6) and the detection limit was 1 ng•mL－1.ConclusionThe method is convenient, of good sensibility and accuracy; it can determine metronidazole in complex oral ulcer paster.
• Select
  药物制剂与药品质量控制

  Study on Extraction Kinetics of Total Flavonoids in Prunella

  WANG Qibin;ZHU Haitao;CHEN Li

  2012, 31(8): 1070-1072.

  Download PDF ( )   Knowledge map   Save

  ObjectiveTo study dynamic changes of total flavonoids extracted from Prunella by using ultrasonic enhancement and hotdip methods and the influence of ultrasonic on the total flavonoids extraction. MethodsThe total flavonoids in Prunella were extracted by ultrasonic enhancement and hotdip methods. We investigated the dynamic changes of total flavonoids in the extraction process. ResultsThe total flavonoids extraction rate of the ultrasound enhanced extraction was 2.73 times that of the hotdip extraction. The extraction rates of ultrasonic enhancement and hotdip extraction were 99.21% and 36.61% respectively at the 260th min. The extraction time was respectively 58 min and 106 min when 70% of total flavonoids were extracted. ConclusionUltrasonic can significantly improve the extraction of total flavonoids from Prunella.