ObjectiveThe ultrafiltration was employed to determine the human plasma protein binding ratio of a novel Caspase inhibitor F1013. The influence factors of this determination were investigated. MethodsThe unbound fractions in plasma were separated with ultrafiltration equipment under different conditions and were determined with high performance liquid chromatography (HPLC). ResultsThe Amicon Ultra0.5 equipment was suitable for researching the plasma protein binding ratio of F1013. The plasma protein binding ratio of F1013 was increased with the increasing of rotation speed and centrifuge time, and it was decreased with prolongated incubation time. ConclusionHuman plasma protein binding ratio of F1013 was influenced by rotation speed, centrifuge time and incubation time. When the ultrafiltration was applied to study the human plasma protein binding ratio of F1013, the best ultrafiltration conditions are that the human plasma spiked with F1013 was incubated in the 37 ℃ water for 1 h, and then put into the Amicon Ultra0.5 equipment, to centrifuge at 14 000 r8226;min－1 for 15 min. The method has satisfying recovery, precision, and a good feasibility.
ObjectiveTo study dynamic changes of total flavonoids extracted from Prunella by using ultrasonic enhancement and hotdip methods and the influence of ultrasonic on the total flavonoids extraction. MethodsThe total flavonoids in Prunella were extracted by ultrasonic enhancement and hotdip methods. We investigated the dynamic changes of total flavonoids in the extraction process. ResultsThe total flavonoids extraction rate of the ultrasound enhanced extraction was 2.73 times that of the hotdip extraction. The extraction rates of ultrasonic enhancement and hotdip extraction were 99.21% and 36.61% respectively at the 260th min. The extraction time was respectively 58 min and 106 min when 70% of total flavonoids were extracted. ConclusionUltrasonic can significantly improve the extraction of total flavonoids from Prunella.