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    药物研究
  • 药物研究
    JIN Yuan;DENG Yu;WANG Guihua;LI Xiaolan;GONG Jianping;HU Junbo
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    ABSTRACTObjectiveTo study the influence of alltrans retinoic acid(ATRA) on acute promyelocytic leukemia cell line(HL60) differentiation and cell cycle distribution and determine the effective pattern by which ATRA inhibits tumor growth. MethodsTo create the HL60 cell line subcutaneous xenograft tumor model in nude mice,which were treated with ATRA through tail vain every other day and the volume of tumor was measured on day 14. In vitro,the expression of CD11b,a differentiation marker,was detected by using immunofluorescence technique,and cell cycle was tested by PI stain assay. ResultsThe volume of xenograft tumor was (0.5±0.6) cm3 in the ATRA group after 14 days’ treatment,which was (1.9±1.7) cm3 in the control group(P<0.05). In vitro,the expression of differentiation marker CD11b was (27.3±3.2)% in the ATRA group and (5.1±3.2)% in the control group(P<0.01)after cultivation for 72 hours. Being cocultured with ATRA for 24 hours,the cell cycle distribution of HL60 was analyzed,G0/G1 phase was(57.6±1.5)% in the ATRA group and(43.0±2.0)% in the control group (P<0.01). ConclusionThe growth of xenograft tumor was inhibited by ATRA through tail vain injection,which may be associated with cell differentiation induction and cell cycle blockage.
  • 药物研究
    YU Xiaocui;HUANG Lijun;LIU Gaofeng;ZHANG Xiangning
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    ABSTRACTObjectiveTo investigate the effects of danhong injection on the activities of five cytochrome P450 enzymes in vitro for clinical combination therapy. MethodsPhenacetin,tolbutamide,dextrorphan,chlorozoxazone,testosterone were incubated as probes with or without danhong injection at low,middle and high concentrations. HPLC method was used to detect the substrate metabolites to study the effects of danhong injection on the activities of five cytochromes P450 enzymes. ResultsIn vitro,the activities of CYP1A2 and CYP2C9 in danhong injection groups were lower than that in the blank group (P<0.05) ; but no significant difference was presented for CYP2D6 ,CYP2E1 and CYP3A4 in danhong injection groups compared with the control group (P>0.05). with the IC50 value and Ki value were 0.54% and 0.226%,respectively. ConclusionDanhong injection remarkably inhibits the activity of CYP1A2 in vitro in a mixedtype inhibition model. It also weakly suppresses the activity of CYP2C9,but not on CYP2D6,CYP2E1,CYP3A4 activities.
  • 药物研究
    LIU Haijun;LIANG Zaifu;ZHAN Lifen;SUN Jinmin
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    ABSTRACTObjectiveTo evaluate the safety of purified snake venom enzyme (PSE). MethodsOne hundred mice were randomly divided into two groups for the median lethal dose (ivLD50 and ipLD50) test through tail vein injection and intraperitoneal injection,respectively. A total of 120 mice and 40 rabbits were randomly divided into four groups for the effects study of the tested groups at different doses. The mice were administered PSE for 90 days continuously and rabbits were treated with PSE for 45 days (every other day). On the 91st day,blood was collected and ALT,BUN and routine blood testing were carried out.The liver and kidney tissues were moved for pathological examination. ResultsThe ivLD50 of PSE was 6.695 U•kg-1,90% confidence interval was 5.434-7.956 U•kg-1; ip LD50 of PSE was 4.599 U•kg-1,90% confidence interval was 3.735-5.462 U•kg-1. Compared with that of the control group,no significant difference (P>0.05) was showed on the function of liver and kidney and blood indicators. ConclusionPSE has low toxicity at the experimental doses. Since the amount of enzyme is 1.25×10-2 U•g-1 in cream,the dosage for adults (0.05 g•cm-1 equivalent to 6.25×10-4 U•cm-1 enzyme activity) is safe and reliable.
  • 药物研究
    ZENG Chunhui;YANG Ke;DENG Jiagang;YANG Bin;XU Mingguang;LI Xianmei
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    ABSTRACTObjectiveTo investigate longterm toxicity of daganqing granules in rats. MethodsThe total of 60 Wistar rats were randomly divided into the high,middle and low dose groups,which were treated with daganqing granules (170.0,85.0 and 42.5 g •kg-1•d-1 crude drug) by intragastric administration,respectively,once a day for 90 days. The general behaviors and physical status,hematology,blood biochemistry,organs coefficient and histopathological analysis were detected in 90 days and 14 days after drug withdrawn. ResultsNo significant difference in body weight was found between treated rats and the blank control despite the rats treated with 170.0 g•kg-1. There were focal moderate fatty infiltration developed in eight rats and diffuse moderate fatty infiltration in one rat by histopathological examination. However,these symptoms subsided in 14 days after medication termination. The serum creatinine was significantly reduced after consecutive 90 days’therapy of daganqing granules compared with that in the control group (P<0.05),while which were reversed to normal in 14 days after supplements termination. There were no significant differences observed between two other treating groups and the control in the general behaviors and physical status,the hematology,blood biochemistry,organs coefficient and histopathological status. ConclusionDaganqing granules are safe by intragastric administration at the dosage in clinical range.
  • 药物研究
    XIE Chang;SHEN Chanjuan;CHEN Shuhe;YE Xiaochuan;ZOU Pengcheng;LIU Yanwen
    ABSTRACTObjectiveTo explore the bioactive constituents of antiinflammatory active site of Smilax china L. for providing evidence for serum pharmacochemistry research. MethodsThe blood migrating constituents of Smilax china L. were determined by comparing the HPLC fingerprints of the aqueous extracts,drug contained sera and blank sera. ResultsFortyfive compounds absorbed in blood were detected,including twentyfive prototype constituents of Smilax china L. and twenty metabolites. ConclusionThese fortyfive constituents in blood could be bioactive components of Smilax china L.,further studies on which could be useful to clarify the bioactive constituents and mechanisms of action of Smilax china L..
  • 药物研究
    CHEN Wei;LI Cunyu;PENG Guoping;ZHENG Yunfeng;LI Hemin;LI Hongyang
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    ABSTRACTObjectiveTo study effects of ultrafiltration on sensitization of shuanghuanglian injection. MethodsInjected on guinea pigs with shuanghuanglian injection after it was ultrafiltrated and detected the 5hydroxy tryptamine of guinea pig’s plasma,contrasted with 0.9% sodium chloride injection group,egg protein group,shuanghuanglian injection group,shuanghuanglian ultrafiltratea add with aluminum hydroxide group,shuanghuanglian ultrafiltratea add with aluminum chloride group,analysised the 5hydroxy tryptamine change levels of each group,which showed the effect of shuanghuanglian injection sensitization based on ultrafiltration. ResultsThere was no significant difference sensitization before and after ultrafiltrated. ConclusionThe factors that caused the sensitization of guinea pigs were not just residual macromolecular,components,it may be related to some micro molecular components in the injection.
  • 药物研究
    ZHANG Yueqin
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    ABSTRACTObjectiveTo study the antiinflammatory effect of tripterygium glycosides. Methods①The capillary permeability in mice:a total of 60 mice were randomly divided into the blank control,model control,tripterygium glycosides at 0.3,0.6,1.2 g•kg-1 doses,10 mg•kg-1 indomethacin groups. Mice were orally administrated for 5 days,later they were intravenously injected with 0.5% evans blue dye solution 0.01 mL•g-1.By the end of experiment,mice were sacrificed and peritoneal lavage fluid was collected for determination of absorbance(A value); ②The carrageenin paw swelling in rats:fifty rats were randomly distributed into blank control,tripterygium glycosides at dose of 0.25,0.50,1.00 g•kg-1,4 mg•kg-1 dexamethasone groups. after the model was established,rats were continuously given intragastric administration for 7 days,and the volume of right hind limb was determined. ResultsThe a value of peritoneal lavage fluid in the blank control,model control,tripterygium glycosides at dose of 0.25,0.50,1.00 g•kg-1 and 4 mg•kg-1 dexamethasone was 0.24±0.16,1.41±0.60,0.99±0.45,0.93±0.45,0.88±0.58,0.52±0.38,respectively,which demonstrated that tripterygium glycosides inhibited capillary permeability of mice.The foot swelling rate in the groups of blank control,tripterygium glycosides at dose of 0.25,0.50,1.00 g•kg-1 and 4 mg•kg-1 dexamethasone was (36.3±13.7)%,(26.3±13.9)%,(37.9±14.5)%,(32.5±12.4)%,(17.2±10.9)%,respectively 5 h after last administration. ConclusionTripterygium glycosides has certain antiinflammatory and swelling reducing effects.
  • 药物研究
    WANG Zhiqiang;ZHANG Meijun;WU Jiyu;WANG Xuebao;HUANG Xuesun;ZHANG Xiuhua
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    ABSTRACTObjectiveTo investigate the binding interaction of levofloxacin and ofloxacin with human serum albumin (HSA)by fluorescence spectrophotometry.MethodsThe interaction of levofloxacin and ofloxacin with HSA was studied by measuring fluorescence quenching spectra,synchronous fluorescence spectra. The type of binding force was estimated according to thermodynamic equation.ResultsAccording to StemVolmer equation and doublereciprocal equation under physiological condition(pH=7.4,37 ℃),the type of fluorescence quenching of levofloxacin was found to be static quenching,the binding constant was 1.46×105 L•mol-1 and the binding sites was 1.11; the type of fluorescence quenching of ofloxacin also was static quenching,the binding constant was 4.31×104 L•mol-1 and binding sites was 1.04. The interaction between Levofloxacin and HSA was mainly driven by hydrophobic force which was the same as Ofloxacin.ConclusionThe fluorescence quenching,combining constants and binding sites of ofloxacin with HAS were less than those of levofloxacin,which provides important information for studing the pharmacological effects,biological characteristics and configuration modification of levofloxacin and ofloxcin with HSA.
  • 药物研究
    LIANG Huamin;LI Chunyan;HUANG Wei;GUO Tingting
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    ABSTRACTObjectiveTo detect levels of NFκB and TNFα expression in gastric mucosa during Helicobacter pylori (Hp) infection,and demonstrate the relationship between IL1β gene polymorphism and gastic cancer. MethodsHp positive patients with gastroduodenal ulcer in 94 cases,through polymorphism analysis and detection,in which IL1B31T/T genotype in 21 cases,C/C genotype in 40 cases,C/T genotype 33 patients. In 34 patients with Hp negative functional gastrointestinal disease patients as control,in which the IL1B31T/T genotype in 7 cases,C/C genotype in 18 cases,C/T genotype 9 patients.ResultsIn gastric corpus of individuals without Hp infection,the expressions of NFκB and TNFα was remarkably lower and similar in different genotype of IL1B31. The expressions of NFκB and TNFα in subjects with Hp infection,however,was upregulated and T/T genotype of IL1B511 was higher,compared with the other genotypes (1.20±0.17 vs 0.87±0.18 and 0.94±0.16;1.10±0.16 vs 0.90±0.15 and 0.97±0.17,P<0.01,respectively). Individuals with Hp infection have a significant higher NFκB activity and IL1B31T/T genotype was more obvious(0.99±0.12,0.89±0.15 and 0.90±0.14,P<0.01).T/T C/T C/C genotype of IL1B31 had remarkable higher expression TNFα and NFκB in Hp infection than normal control group (P<0.01).ConclusionHp infection promotes TNFα and NFκB mRNA expression,the upregulation of NFκB activity,in the IL1B31T/T genotype is more obvious .After Hp infection,IL1B31 genotype in patients with high risk of gastric cancer has involved in inflammatory reaction.
  • 药物研究
    LIU Yongfang;LIU Zhigang;FENG Xinmin
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    ABSTRACTObjectiveTo investigate the effect of sevoflurane on vascular endothelial growth factor (VEGF)expression and associated signaling pathway in cardiac myocytes of neonate rats.MethodsPrimary cultures of cardiomyocytes of neonatal SD rats born for 1-3 days were incubated with different concentrations sevoflurane (group S1,2,3)at 0.5,1.0,1.5 MAC; with p38MAPK inhibitor SB203580 (group P)at a final concentration of 50 nmol•L-1; with 50nM SB203580 and 1.0 MAC sevoflurane(group SP),respectivly for 6 hours.And the group of cells without any treatment was considered as the control group(group C).VEGF expressions were identified by enzymelinked immunosorbent assay.ResultsSevoflurane dose dependently increased cardiac myocytes VEGF expression.①The VEGF levels were significantly raised in group S2(123.32±11.56)and group S3(177.46±31.03)(P<0.05 or P<0.01)compared with those in the group C (61.09±6.21)and group S1 (73.67±8.02) pg•mL-1(P<0.01).②The upregulation of sevoflurane on VEGF was blocked by SB203580,however,the latter did not alter VEGF secretion from cardiomyocytes of neonatal rats (P>0.05).Compared with group S2,VEGF declined in cells of group SP and remained unchanged in goup P(P>0.05).ConclusionInducing VEGF expression by sevoflurane via p38MAPK signal pathway may be associated with its cardiac protection.
  • 抗结核病药物专栏
  • 抗结核病药物专栏
    DU Youyi;XIONG Xuefang;ZHOU Jiafeng
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    ABSTRACTObjectiveTo study the clinical efficacy of pemetrexed combined with oxaliplatin to treat patients with advanced nonsmall cell lung cancer and the impact of which on serum carbohydrate antigen (CA) and carcinoembryonic antigen(CEA). MethodsA total of 60 patients with advanced nonsmall cell lung cancer were randomly divided into the treatment group and the control one,30 in each group. The treated group received 500 mg•(m2)-1 of pemetrexed and 120 mg•(m2)-1 of oxaliplatin by intravenous infusion on the first day,and repeated for every 3 weeks; the control group received 1 000-1 250 mg•(m2)-1 of gemcitabine by intravenous infusion on the first and eighth day and 120 mg•(m2)-1 of oxaliplatin by intravenous infusion on the first day,and repeated every 3 weeks. The tumor foci,the quality of life (body weight,Karnofsky score),CA125 and CEA levels were compared between two groups after two courses of treatment. ResultsThere were no significant differenes in efficacy and tumor stability between two groups (P>0.05) by the end of treatment; the body weight and Karnofsky score of the treated group and the control group were (74.06±5.28) kg and(67.94±5.03) kg,(84.63±8.26) and(76.85±7.94) (P<0.05),respectively; CA125 and CEA levels were significantly decreased compared with those prechemotherapy (P<0.05 or P<0.01),and which declined more conspicuously in the treated group than those in the control group (P<0.05) after treatment. ConclusionCombining pemetrexed with oxaliplatin for patients with advanced nonsmall cell lung cancer is a safe and effective alternative chemotherapy,which not only improves the quality of life,but also reduces the serum level of CA125 and CEA.
  • 抗结核病药物专栏
    LI Juan;MA Rongli;ZHANG Yan
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    ABSTRACTObjectiveTo evaluate the effects of linezolid on platelet in the process of treating elderly patients with gramnegative bacteria affected lung infection.MethodsClinical data of 13 elderly patients with lung infection who were treated with linezolid were analyzed retrospectively.ResultsThere were 7 elderly patients developed thrombocytopenia,and the incidence of which was 53.85%.The reduction of platelet developed on the third to twelfth day of linezolid usage,and the lowest platelets count occurred on the sixth to twelfth day.There were 2 cases of thrombocytopenia with bleeding complications.All the 7 cases terminated using linezolid,in which there were 2 cases infused with platelet and 1 with plasma.The platelets counts rose again on the third to sixth day after linezolid withdrawn,and the platelet resumed to the normal level on the sixth to fourteenth day.ConclusionWhen elderly inpatients with lung infection receive linezolid against gramnegative bacteria,the incidence of thrombocytopenia turns high.In clinical settings,we should choose linezolid carefully and monitor platelet counts closely in the course of using linezolid and posttreatment.
  • 药物与临床
  • 药物与临床
    SU Wei;NA Wanqiu;LI Jianhua;YANG Jianhong;XIA Qingchun;GUAN Tiefeng
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    ABSTRACTObjectiveTo investigate the clinical efficacy for treating mild and moderate ischemic poststroke depression in aged patients and effect on neural dysfunction of shuganjieyu capsule. MethodsA total of 84 aged patients with mild and moderate ischemic poststroke depression were randomly assigned to the test group and the control group (n=42,each).The test group was treated with shuganjieyu capsule (0.72 g,bid),and the control group was treated with citalopram tablet (20 mg,qd) for 6 weeks.The therapeutic efficacy,adverse reactions and neurological dysfunction were evaluated at the beginning and by the end of 2,4,6week after treatment by Hamilton Depression Scale(HAMD),Hamilton Anxiety Scale(HAMA),Clinical Global Impression(CGI),Treatment Emergent Symptoms Scale(TESS),and Chinese Scale of Clinical Neurologic Deficit (CSS),respectively. ResultsBy the end of study,the efficacy of the test group and the control group was 69.0% and 64.3%,respectively(P>0.05). And the scores of HAMD and HAMA were significantly lower than those at the start(P<0.05),and no statistically significant difference was found between two groups(P>0.05). The TESS was conspicuously fewer in the test group than that in the control group (P<0.01),while the efficacy index of which was remarkably superior than that of the control group (P<0.01). By the 6th weekend,the CSS was significantly lower than that at the beginning and that in control group (P<0.05). ConclusionShuganjieyu capsule presents the similar effect as citalopram on treating mild and moderate poststroke depression in aged patients with less side effects and promotes neurological function recovery.
  • 药物与临床
    CHEN Shiming;WANG Yufang
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    ABSTRACTObjectiveTo investigate the clinical efficacy by combination medication of cyclosporine,methylprednisolone and gamma globulin on severe aplastic anemia (SAA). MethodsFiftyfour cases of severe aplastic anemia were randomly divided into the test group (27 cases) and the control group (27 cases). The controls were only treated with androgen as conventional therapy,and the test group was treated with cyclosporine A(CsA,3.5-12.0 mg•kg-1 •d-1),highdose of methylprednisolone (HDMP,1 000 mg•d-1,60 mg•d-1) and highdose of gamma globulin (HDIVIG,20 g•d-1) in addition of the conventional therapy. The treatment lasted for three months,and blood parameters were checked. ResultsThe total effective cases were 21 in the test group and 14 in the control(P<0.05). Compared with the control,blood indicators of the test group were all significantly elevated after treatment(P<0.05),and no serious adverse reactions occurred in two groups during the treatment. ConclusionThe combination medication of cyclosporin A,methylprednisolone and gamma globulin with androgen shows significant effect on severe aplastic anemia,less side effects and is worth developing into clinical application.
  • 药物与临床
    LUO Junfeng
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    ABSTRACTObjectiveTo study the clinical efficacy of congrong yishen granula combination on treating chronic prostatitis accompanied with sexual dysfunction.MethodsA total of 120 patients with chronic prostatitis accompanning sexual dysfunction were divided into test group (64)and control group (56).Fiftysix in the control group were treated with oral levofloxacin tablets 0.2 g,tid and tamsulosin hydrochloride sustainedrelease tablets 0.2 mg,qd for 8 weeks.Sixtyfour in the therapy group were cotreated with oral congrong yishen granula1sack,tid,besides administration of levofloxacin and tamsulosin hydrochloride in the same way as above for 8 weeks.The sexual function between pretherapy and posttherapy were compared according to chronic prostatitisrelated sexual function index (CPSFI)form and international index for erectile function(IIEF5)questionnaires.ResultsThe totol effective rate by CPSFI form of the test group and control one was 90.6% and 53.6%,respectively.The totol effective rate in the test group and control by using IEF5 questionnaires was 84.4% and 57.1%,respectively.There was significant difference between two groups(P<0.01).ConclusionIn combination with congrong yishen granula for treating chronic prostatitis accompaied with sexual dysfunction could generate satisfying effect.ABSTRACTObjectiveTo study the clinical efficacy of congrong yishen granula combination on treating chronic prostatitis accompanied with sexual dysfunction.MethodsA total of 120 patients with chronic prostatitis accompanning sexual dysfunction were divided into test group (64)and control group (56).Fiftysix in the control group were treated with oral levofloxacin tablets 0.2 g,tid and tamsulosin hydrochloride sustainedrelease tablets 0.2 mg,qd for 8 weeks.Sixtyfour in the therapy group were cotreated with oral congrong yishen granula1sack,tid,besides administration of levofloxacin and tamsulosin hydrochloride in the same way as above for 8 weeks.The sexual function between pretherapy and posttherapy were compared according to chronic prostatitisrelated sexual function index (CPSFI)form and international index for erectile function(IIEF5)questionnaires.ResultsThe totol effective rate by CPSFI form of the test group and control one was 90.6% and 53.6%,respectively.The totol effective rate in the test group and control by using IEF5 questionnaires was 84.4% and 57.1%,respectively.There was significant difference between two groups(P<0.01).ConclusionIn combination with congrong yishen granula for treating chronic prostatitis accompaied with sexual dysfunction could generate satisfying effect.
  • 药物制剂与药品质量控制
  • 药物制剂与药品质量控制
    ZOU Jiangbing;KONG Qiuling;CHEN Longhao;JIANG Linlan
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    ABSTRACTObjectiveTo develop a method to determinate total flavonoids in Passiflora edulis Sims from different areas. MethodsThe total flavonoids in Passiflora edulis Sims were determined by spectrophotometry at 273 nm. Rutin was used as standard. A mixture of AlCl3NaAC solution was taken as a color reagent. ResultsThe calibration curve was linear in the range of 9.94-49.68 μg•mL-1(r=0.999 9).The average recovery was 99.71%(RSD=1.32%).The contents of total flavonoids from different areas were 1.150%-2.842%. ConclusionThere is variation for the total flavonoids from Passiflora edulis Sims planted in different areas.
  • 药物制剂与药品质量控制
    Xirali TURSUN;Mardan NIYAZ;Kurbannisa MATKASIM;Turghun KADIR;Gulxan MUHAMMAT;Hayrinsagul;Kaiser ABDUKIRIM
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    ABSTRACTObjectiveTo develop a HPLC method for determination of gallic acid in Amilenushidari majun. MethodsThe column of phenomenex C18(4.6 mm×250 mm,5 μm) was used in HPLC,and the methanol0.4%phosphoric acid (10:90) was taken as mobile phase,the column temperature was kept at 25 ℃ and detection wavelength was set at 273 nm. A flow rate was set at 1.0 mL•min-1 and 10 μL sample was loaded. ResultsGood linear relation between peak area and loading quantity was obtained in the range of 0.106-0.530 μg (r=0.999 7)of gallic acid ,and the average recovery of gallic acid was 97.9%(n=9),RSD=1.13%. ConclusionThis method is simple,rapid,accurate and reliable for the quality control of Amilanoshidari majun.
  • 药物制剂与药品质量控制
    WANG Wenqing;LI Wei;HOU Xiaolong;FANG Jianguo
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    ABSTRACTObjectiveTo establish a method for content determination of polysaccharide in shengu granule for quality evaluation. MethodsThe content of polysaccharide was determined by phenolsulfuric acid method and UV photometry at 486 nm. ResultsGood linearity,high accuracy and precision,wide range and long durability of calium were obtained in this way. The average recovery of high,middle and low content was 97.85%,99.99%,98.35%,respectively(n=9). ConclusionThis method is sensitive,accurate and rapid,which can be used for quality control of calium in shengu granule.
  • 药物制剂与药品质量控制
    LIU Jinmei;ZHAI Xuejia;SHI Fang;CHEN Dongsheng;LU Yongning
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    ABSTRACTObjectiveTo develop a HPLC method for the simultaneous determination of protocatechuic acid,protocatechuic aldehyde and paeoniflorin in compound chuanji tablets. MethodsA Thermo ODS2 HYPERSIL C18 column (250 mm×4.6 mm,5 μm) was used. The mobile phase consisted of 10 mmol•L-1 KH2PO4,0.02% H3PO4 in water (A) and methanol (B) with linear gradient elution (0-12 min,15% B; 12-32 min,25% B),The flow rate was 1.0 mL•min-1. DAD detection was used and detection wavelength was set at 210 nm for protocatechuic acid,280 nm for protocatechuic aldehyde and 232 nm for paeoniflorin,respectively. The temperature of column was 38 ℃. ResultsProtocatechuic acid,protocatechuic aldehyde and paeoniflorin showed good linearity (r≥ 0.999 9) in the range of 5.27-105.50,1.60-31.92 and 86.04-1 721.00 μg•mL-1,respectively. The RSDs of intraday and interday precision were no more than 1.93% (n=6). The average recovery rate of three tested compounds at low,medium and high concentration levels were in the range of 98.39%-99.94% for all with RSDs less than 1.78% (n=6). The contents of protocatechuic acid,protocatechuic aldehyde and paeoniflorin in compound chuanji tablets were (120.70±1.94),(24.28±0.57),(3 439.00±60.18) μg•g-1,respectively. ConclusionThe developed method is simple,accurate,reliable and can be used to control the quality of compound chuanji tablets.
  • 药物制剂与药品质量控制
    ZHANG Qian;ZHU Libin;HUANG Zhijun;ZHAI Li;LI Chongming
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    ABSTRACTObjectiveTo establish the method of quality control for liujun shengfa tablets. MethodsAngelica sinensis,Cacumen Platycladi,Royal jelly powder in liujun shengfa tablets were idendified by TLC.The 2,3,5,4’tetrahydroxystilbene2OβDglucoside contained in tablets were determined by HPLC. ResultsThe qualitative identification with TLC was specific and simple to be operated. It was showd that a good linear correlation appeared in the range of 0.045 6-0.912 0 μg (r=0.999 9) of 2,3,5,4’tetrahydroxystilbene2OβDglucoside,and the average recovery was 98.99% (RSD= 1.99%,n=6). ConclusionThe method is simple,accurate and reliable,which can be used for quality control of liujun shengfa tablets.
  • 用药指南
  • 用药指南
    QIAO Xiaoyun;WANG Yu;PEI Yunping
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    ABSTRACTObjectiveTo study the optimal monitoring indicators for assessment of plasma cyclosporine A (CsA) in diabetic nephropathy (DN) patients receiving renal transplantation. MethodsThe lowest and highest concentration (C0,C2) of CsA in 17 DN patients receiving renal transplantation were detected by Enzymemultiplied immunoassay technique(Emit). The data about liver toxicity,renal toxicity or rejection were eliminated and others were grouped and analyzed according to the time afteroperation,gender and age. ResultsThe rational range of (C0+C2) of CsA in the first month,second to fourth month,fifth to sixth month after renal transplantation were 800-1 800 ng•mL-1,700-1 500 ng•mL-1,600-1 200 ng•mL-1,respectively,while C2/C0 were within 4~8. ConclusionUsing C0+C2 and C2/C0 as monitoring indicators for the plasma concentration of CsA,comprehensively reflects the drug exposure in body and toxicity of CsA to liver and kidney,and benefits for avoiding rejection and toxic effects.
  • 用药指南
    WANG Jinqiu;WANG Min;FENG Yanmei
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    ABSTRACTObjectiveTo guide a reasonable medication for epileptic patients. MethodsThe total of 3 906 cases of epileptic patients administrated with valproic acid(VPA) and carbamazepine(CBZ) were followed up in No.2 hospital of Xi’an Jiaotong University from 2007 to 2010,in which 100 samples were taken out for monitoring the serum freedrug concentration. ResultsThe concentration of VPA and CBZ used in combination or alone decreased(72.5% vs 54.8%) and increased(58.0% vs 84.3%),respectively. However,at the higher concentration,the protein biding rate was significantly reduced and the free serum level was increased. ConclusionThe dosage should be adjusted according to the serum drug monitoring when VPA and CBZ were used together,and the free serum drug concentration should be under supervision at a high dose.