2012, 31(12): 1613-1615.
ABSTRACTObjectiveTo establish a high performance liquid chromatography （HPLC) method for quantitative determination of fluticasone propionate in percutaneous solution，and investigate the ability of percutaneous penetration of fluticasone propionate cream.MethodsHypersil ODS C18 column （250 mm×4.6 mm，5 μm) was used. Mobile phase was as follows: methanol1.15 g·L－1 ammonium dihydrogen phosphate （adjusted to pH 3.5)acetonitrile （55：35：15 in volume). The column temperature was set at 55 ℃. The flow rate was 1.0 mL·min－1. The detection wavelength was 240 nm. Improved Franz type diffusion cells were used in vitro permeation studies and excised minipig’s skins in vitro were used as a transdermal barrier. The concentration of fluticasone propionate in the receptor solution was determined by HPLC to investigate its cumulative permeation quantities at different time points respectively，which were compared with those of the commercial cream. ResultsThe regression equation of penciclovir was linear in the range of 0.050 2－1.003 0 μg·mL－1 （A＝47 709.364 7×C＋300.774 4，r＝0.999 8). The average recovery was 99.58%. A good linear relationship existed between the cumulative penetration quantities and the time. There was no significant difference in the skin permeation quantities between the selfmade cream and commercial cream. ConclusionThe method was simple，rapid，and accurate. It is a better method to evaluate the characteristics of permeation for fluticasone propionate.