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    药物研究
  • 药物研究
    DING Xiaowei;SHI Lanpeng;JIANG Daomeng;XU Xiao yan;ZHOU Han;QI Can;XIONG Zhentian;MA Tao*
    2011, 30(12): 1546-1548. https://doi.org/10.3870/yydb.2011.12.002
    ABSTRACTObjectiveTo investigate the effects of haloperidol (H) on conditional place preference induced by ketamine (K) in mice.MethodsForty mice enrolled were randomly divided into 4 groups (n= 10):control group (NS group),ketamine group (K group),ketamine plus haloperidol (0.33 mg•kg1) group (KH1 group) and ketamine plus haloperidol (1.0 mg•kg1) group (KH2 group).The conditional place preference (CPP) tests were carried out in each mouse and the duration of staying in the white box 24 hours before and after withdrawal.ResultsThe basic duration for 24 hours before withdrawal in group NS,K,KH1 and KH2 was (157.38±68.75),(197.78±90.16),(190.11±127.47) and (244.00±127.88) s,respectively; that for 24 hours after withdrawal was (316.25±263.52),(337.44±152.92),(324.44±172.61)and (317.56±165.85) s,respectively.Compared with the basic duration within group,the duration of staying in the white box after withdrawal for group K or group KH1 prolonged (P<0.05),but no statistically significant difference (P>0.05) was found for group KH2.ConclusionHaloperidol may interfere with the conditional place preference induced by ketamine in mice,which implies that it may lessen the psychic dependence caused by ketamine.
  • 药物研究
    ZHOU Junhua;XIONG Zhe
    2011, 30(12): 1549-1551. https://doi.org/10.3870/yydb.2011.12.003
    ABSTRACTObjectiveTo explore the antidepressant effects of picrosideⅡ and possible mechanism.MethodsNinetysix male SD rats were radomly divided into 6 groups.The normal and model controls were treated with normal saline.The others were i.p.administrated with picrosideⅡ at doses of 1,3,10 mg•kg1 and imipramine at 15 mg•kg1,respectively for 14 d.Chronic unpredictable mild stress model (CMS)was established and the behavior was observed by the forced swimming test (FST)and open field test (OPT).The levels of adrenocorticotropic hormone (ACTH)and corticosterone (CORT)in blood serum were detected by radioimmunoassay.ResultsAfter 21 days,compared with the normal control,the model control showed longer immobility duration,lower crossing and rearing scores,higher concentration of ACTH and CORT in blood serum (P<0.05).While,the immobility time was shortened and crossing and rearing scores was improved and the concentration of ACTH and CORT in blood serum were reduced for rats treated with three doses of picrosideⅡand imipramine for 14 days,respectively compared with those in the model group(P<0.05).ConclusionPicrosideⅡ ameliorated behavioral injury caused by depression,which might be associated with the regulation of plasma levels of ACTH and CORT.
  • 药物研究
    LIU Wenxian;CHEN Qingxia;ZHOU Guicheng;LIU Weizhong;WEN Yuguan;WANG Guangfa
    2011, 30(12): 1552-1555. https://doi.org/10.3870/yydb.2011.12.004
    ABSTRACTObjectiveTo establish a method for determining the concentration of sertraline and aripiprazole in human plasma by HPLC.MethodsThe C18 column(150 mm×4.6 mm,5 μm)was used in the reversed HPLC system,the mobile phase consisted of 0.03 mol•L1 ammonioummethanol(18:82); the flow rate was 0.8 mL•min1; the detection wavelength was at 220 nm.Ethyl acetatedichloromethane(80:20)were used as extracting solvent.ResultsThe calibration curves were linear in the range of 20.0-640.0 ng•mL1 for sertraline,25.0-1 000.0 ng•mL1 for aripiprazole,respectively.The average recoveries of sertraline and aripiprazole at low,middle and high concentrations were 101.30%,98.14%,97.92% and 101.60%,97.75%,98.16%,respectively; the extraction recovery were 70.56%,73.87%,76.45% and 71.66%,74.12%,75.26%,respectively.The intraday and interday variations(RSD)were both less than 10% (n=5).The minimum detectable concentration of method was 10.0 ng•mL1. The calibration curves of sertraline and aripiprazole showed good linearity,being equal to 0.998 6 and 0.998 9.The regression equation of sertraline was Y=92.251X+1.61 and Y=85.489X+1.27 of aripiprazole,respectively.ConclusionThe method is sensitive,accurate,simple and quick,which can be used for clinical drug monitoring and pharmacokinetics studies of sertraline and aripiprazole.
  • 药物研究
    SHU Chengren;HUANG Lu;GE Miaomiao;LI Weiyong
    2011, 30(12): 1555-1558. https://doi.org/10.3870/yydb.2011.12.005
    ABSTRACTObjectiveTo evaluate the pharmacokinetics of dexmedetomidine hydrochloride injection in Chinese healthy volunteers.Methods30 healthy volunteers were divided into three groups: intravenous administration of dexmedetomidine at 0.5,1.0 and 1.5 μg•kg1.Concentrations of dexmedetomidine in plasma were determined by HPLC/MS/MS.The pharmacokinetics parameters were calculated with DAS 2.0 software.ResultsThe main pharmacokinetic parameters for three groups were as follows:AUC0→t were (902.51±264.18),(1 828.68±643.71)and (2 462.55±492.97) pg•h•mL1;AUC0→∞ were (1 068.87±505.46),(2 027.72±816.93) and (2 597.89±463.06) pg•h•mL1;Cmax were (569.90±155.87),(1 042.80±330.77) and (1 682.00±639.67) pg•mL1;tmax were (0.13±0.03),(0.08±0.00)and (0.11±0.04) h;t1/2 were (2.69±1.38),(2.59±1.12) and (2.62±0.87) h, respectively.There were no significant differences in AUC0→12,AUC0→∞,Cmax,t1/2,tmax,CLz,Vz among three groups by variance analysis(P>0.05).ConclusionThe pharmacokinetic parameters of dexmedetomidine hydrochloride such as AUC0→12,AUC0→∞,Cmax increased in a dosedependent manner in healthy human,which is consistent with linear kinetic process.
  • 皮肤病性病用药专栏
  • 皮肤病性病用药专栏
    WANG Li;CAO Yi;YANG Xiaohong
    2011, 30(12): 1559-1561. https://doi.org/10.3870/yydb.2011.12.006
    ABSTRACTObjectiveTo investigate the effects and possible mechanism of curcumin on proliferation and apoptosis of HaCaT cells. MethodsThe inverted microscope was used to observe morphology of HaCaT cells cocultured with different concentrations of curcumin; the MTT assay was taken to check cytotoxic effect of curcumin on HaCaT cells;the flowcytometric analysis was carried out to test extent of apoptosis;Western Blot was applied to examine Bcl2 and Bax protein expression. ResultsThe morphology of cells treated with curcumin changed in a dosedependent manner and part of cells fell off from the vial. No significant difference in MTT assay was showed between group of curcumin at 0.1 μmol•L-1 and the control (P>0.05),but it was shown for the group of curcumin over 0.1 μmol•L1;Flow cytometry results showed that the apoptosis of HaCaT cells was dosedependently raised by curcumin;the expression of Bax was elevated and Bcl2 was dropped. ConclusionCurcumin inhibits HaCaT cells proliferation and elicits their apoptosis,the mechanism of which may be related to the regulation of Bcl2 and Bax expression.
  • 皮肤病性病用药专栏
    YANG Jishun;XU Liping;HU Jinhong
    2011, 30(12): 1562-1564. https://doi.org/10.3870/yydb.2011.12.007
    ABSTRACTObjectiveTo explore the therapeutic mechanism of sulfur mustard (SM)on guinea pigs with psoriasislike skin lesions.Methods5% propranolol cream was evenly applied on ears of guinea pigs twice a day for 28 d.Since day 21 guinea pigs were cotreated with sulfur mustard paste.The thickness of guinea pig’s ears was tested,and skin pathological changes were detected by HE staining.The expression level of TRAIL was determined by immunohistochemical staining.ResultsSulfur mustard ameliorated ear swelling of guinea pigs(P<0.05).It reversed the injured skin to normal and promoted the TRAIL expression in sera conspicuously vs the control group.ConclusionTherapeutic mechanism of sulfur mustard for psoriasis could be related to inducing overexpression of TRAIL,and promoting apoptosis of skin mutant cells.
  • 皮肤病性病用药专栏
    FENG Jianbing;WANG Shuqin;PENG Yuanxiang;LIN Nengxing
    2011, 30(12): 1565-1567. https://doi.org/10.3870/yydb.2011.12.008
    ABSTRACTObjectiveTo study the effect of treating proriasis vulgaris with calcipotriol ointment plus mometasone furoate cream.Methods180 patients with proriasis vulgaris were randomly divided into three treatment groups,group A (60 cases) were treated with mometasone furoate cream, group B (60 cases) were treated with calcipotriol ointment, and group C (60 cases) were treated with calcipotriol ointment and mometasone furoate cream, the duration of treatment was 4week and followup was 3month. The therapeutic effect, safety and recurrence were recorded. ResultsThe curative rates of three ways were 30.0%(group A), 33.3%( group B)and 56.7% (group C), the efficiency were 43.3%( group A), 46.7%( group B),and 33.3%(group C), the efficacy were 73.3%( group A),80.0%( group B),and 90.0%( group C), respectively (P<0.05). The recurrence of group B and group C were significantly lower than that of group A. All cases were safe during process of treatment. ConclusionCombination of calcipotriol ointment and mometasone furoate cream is effective and safe for the treatment of psoriasis vulgaris.
  • 药物与临床
  • 药物与临床
    DAI Wei;WENG Fangzhong;ZHOU Ruixiang;YAN Jun;FAN Xuepeng
    2011, 30(12): 1570-1573. https://doi.org/10.3870/yydb.2011.12.010

    ABSTRACTObjectiveTo investigate the effects of glutamine(Gln)associated with enternal nutrition supplement on patients with severe pulmonary infection. MethodsSixty patients with severe pulmonary infection were randomly divided into two groups: group A(standard enternal nutrition supplement),group B(standard enternal nutrition supplement+Gln)(5.0 g,nasogastric feeding,tid). The enteral nutrition was given through nasogastric tube by using a pump in both groups,from 500 mL•d1 to 1 000 mL•d1 gradually(in 3 or 4 days ),at 80~100 mL•h1.Prior to treatment and 7 and 14 days after treatment,blood samples were collected for analyzing glutamic acid,glutamine,albumin(ALB),prealbumin(PAB),Creactive protein (CRP),immunonlobulin G(IgG),interleukin6 (IL6),tumor necrosis factorα(TNFα),amount of total peripheral lymphocytes and CD+3,CD+4,CD+4/CD+8. The scores of acute physiology and chronic health evaluation II (APACHE II) and multiple organ dysfunction syndrome(MODS),the durations of intensive care unit(ICU) stay,mechanical ventilation and 28 day survival rate were compared. ResultsThe Tcell subsets and number of total lymphocytes(14 days after treatment) in group A and B were (1.37±0.23)×109 and (1.95±0.29)×109•L1. The levels of serum CRP,IL6,TNFα in group A and B in 14 days were CRP (263.4±18.5) and (137.5±11.3) mg•L1;(77.6±32.6) and (62.4±22.5) pg•L1;(31.7±12.7) and (24.3±11.4) pg•L1,respectively. APACHE II and MODS scores in group A and B by the same time were 17.88±3.27 and 14.37±3.82;8.81±2.57 and 7.72±2.41. CD+3,CD+4 and CD+4/CD+8 in group B were conspicuously raised(P<0.05).There were no significant differences in the durations of ICU stay,mechanical ventilation and 28 day survival rate between two groups(P>0.05).ConclusionGlutamine(Gln)plus enternal nutrition remarkably improve immune function of patients with severe pulmonary infection,and promote infection control.

  • 药物与临床
    CHEN Jun;LUO Rui
    2011, 30(12): 1574-1575. https://doi.org/10.3870/yydb.2011.12.011
    ABSTRACTObjectiveTo evaluate the efficacy of administering dezocine plus anisodamine for renal colic. MethodsThe total of 160 patients with renal colic were divided into two groups randomly. Patients in group A were given intravenously with 10 mg dezocine and 10 mg anisodamine; those in group B were instilled with 5 mg dezocine and 10 mg anisodamine. The curative effect and adverse reaction were recorded and compared between two groups. ResultsThe efficacy in group A and B was 85.0% and 67.5%,respectively,and total effective rate of which was 98.8% and 97.5%,respectively. The side effects were rarely occurred and mild. ConclusionTreatment of dezocine plus anisodamine for renal colic is a safe and effective way. Ten mg of dezocine may relieve the pain faster than 5 mg one.
  • 药物与临床
    LIU Wenbing;DING Xuejun;LOU Liming;HU Dandan
    2011, 30(12): 1576-1578. https://doi.org/10.3870/yydb.2011.12.012
    ABSTRACTObjectiveTo study the effect of Ginkgo biloba extracts (GBE) on plasma 8  iso prostaglandin (8isoPGF2α),superoxide dismutase (SOD) and blood gas of patients with chronic obstructive pulmonary disease at exacerbation (AECOPD) stage. MethodsThirty healthy people as control,60 patients with AECOPD (test group) were selected,the latter were randomly divided into treatment group (GBE + conventional treatment) and control group (conventional treatment),30 cases in each. The patients in the treatment group were given with 20 mL GBE as infusion diluted in 250 mL glucose solution intravenously,once daily besides conventional treatment for 2 weeks. The clinical indicators as 8isoPGF2α,SOD,blood gas before and after treatment were measured and compared. ResultsThe plasma 8isoPGF2α in patients with AECOPD and control was (158.73±36.78) and (37.56±23.45) pg•mL1; SOD was (83.30±7.35) U•mL1 and (101.30±6.90) U•mL1,respectively,before treatment. The plasma 8isoPGF2αin the treatment group and control after treatment was (40.62±7.69) pg•mL1 and ( 96.74±10.24) pg•mL1,SOD was (98.20±2.52) and(90.80±7.24) U•mL1,respectively. ConclusionGBE is benefit for ameliorating oxidative stress in patients with AECOPD,and increases antioxidative capacity of patients.
  • 药物与临床
    YU Liangsheng;XIE Lijin;LI Donglai
    2011, 30(12): 1579-1582. https://doi.org/10.3870/yydb.2011.12.013
    ABSTRACTObjectiveTo study the pretreatment with hydroxyethyl starch on the maternal hemodynamics and oxygen supplydemand balance in fetus during cesarean section under combined spinalepidural anesthesia (CSEA).Methods60 patients undergoing elective cesarean section under CSEA(L3~4),were randomly divided into hydroxyethyl starch group (Group HES,n=30)and sodium lactate ringer’s solution group (Group LR,n=30).The patients were infused IV with hydroxyethyl starch or sodium lactate ringer’s solution 500 mL,respectively before anesthesia.The SBP,DBP,HR and SpO2 were monitored and recorded at the following timepoints:entering the operating room (T0),the end of prefilling liquid infusion (T1),5 min after subarachnoid anesthesia (T2),fetus being born(T3),and the end of operation (T4).The difference of blood pH value (pHva),partial pressure of oxygen (PvaO2),saturation of oxygen (SvaO2),content of oxygen (CvaO2)and fetal oxygen absorbing rate (ERO2)in arterial and venous blood were calculated according to blood gas analysis,which were achieved from collecting fetus umbilical arterial and venous blood when the fetus was born,and the oxygen supplydemand balance in fetus were assessed comprehensively.The Apgar scores of neonates were valued.ResultsCompared with Group HES,the values of SBP or DBP from T2 to T4 significantly decreased and HR were significantly faster in Group LR(P<0.05).The incidence of hypotension and amount of ephedrine used in Group LR were significantly higher than those in Group HES,respectively (P<0.05).Compared with those in Group LR,the blood pH in fetal umbilical arterial and venous blood,PvO2,SvO2 and CvO2 in umbilical vein,PvaO2,SvaO2,CvaO2 and ERO2 in both fetal umbilical arterial and venous blood in Group HES were all obviously higher [pH:(7.29±0.03)vs (7.26±0.06),PvO2:(4.23±0.61)vs (3.26±0.68) kPa,SvO2:(67.2±10.5)% vs (56.3±12.0)%,CvO2:(170.4±14.0)vs (122.6±13.3) mL•L-1,PvaO2:(1.62±0.50)vs (0.92±0.85) kPa,SvaO2:(33.1±7.2)% vs (22.5±9.0),CvaO2:(86.3±11.6)vs (53.4±16.5)%,ERO2:(51.5±6.3)vs (44.2±8.9)%,P<0.05].While the fetus was delivered,there was no consipicuous difference in Apgar scores of fetus between two groups.ConclusionPretreatment with hydroxyethyl starch could maintain the hemodynamic stability of mothers during cesarean section under CSEA,increase the oxygen supply to fetus and improve uptake and utilization of oxygen for fetus.
  • 药物制剂与药品质量控制
  • 药物制剂与药品质量控制
    LI Ke;GAO Yunsheng;LI Binglong;QI Yongxiu
    2011, 30(12): 1633-1636. https://doi.org/10.3870/yydb.2011.12.029
    ABSTRACTObjectiveTo establish a HPLCELSD method for determining cholesterol and making a comparation of which between two kinds of calculus bovis.MethodsA C18(250 mm×4.6 mm,5 μm)column was used with a mobile phase as acetonitrile Isopropanol (1:1)flowing at 1.0 mL•min1.The temperature of drift tube was 67.5 ℃,and the gas flow rate was 1.7 L•min1.ResultsThe cholesterol was in a good linear relationship in the range of 4.0-20.0 μg•mL1,logA=1.541 2logC-2.525 (r=0.999 2).The average recovery was 98.41%,100.04%,96.46%,respectively, and RSD was 0.63%,1.67%,1.03%,respectively.ConclusionThe method is simple,accurate,specific and can be used for the quality control of cholesterol in natural calculus bovis,succedaneums and preparations.
  • 药物制剂与药品质量控制
    HE Jin;SHI Guobing;GAO Jun;LI Junying
    2011, 30(12): 1636-1638. https://doi.org/10.3870/yydb.2011.12.030
    ABSTRACTObjectiveTo establish a method for checking bacterial endotoxin in solution of boric acid.MethodsThe method was based on the bacterial endotoxin test described in the appendix of Chinese Pharmacopeia 2010,including the sensitivity of tachypleus amebocyte lysate (TAL),preliminary interference test and official interference test of bacterial endotoxin. ResultsThe interference was eliminated by using 0.125 EU•mL1 TAL in boric acid solution being diluted by 4 times.ConclusionThe method is simple and reliable,which can be used for checking bacterial endotoxin in boric acid solution.
  • 药物制剂与药品质量控制
    LIN Lisha;GE Jian;YANG Jingjing;HAN SHUAI Weiqi;DENG Lijuan;HAN Baoyu
    2011, 30(12): 1639-1641. https://doi.org/10.3870/yydb.2011.12.031
    ABSTRACTObjectiveTo establish a method for determination of three kinds catechin in tea and compare content of which from different species of tea in current market.MethodsThe content of three kinds of catechin was measured by modified HPLC method and difference of which was tested.The determination was performed on Hypersil BDS C18(250 mm×4.6 mm,5 μm)column with acetonitrile0.1% citric acid (10:90)as mobile phase at detection wavelength of 280 nm.And the column temperature was set at 30 ℃ and the wave length was 280 nm.ResultsThe good linear relationship of three kinds of catechin occurred in the range of 2-400 mg•L1 (r>0.999).And the average recoveries were more than 99.0% and RSD were less than 2.0%.ConclusionThere was great difference in the content of catechin from five species of tea. It is suggested to further study and complete picking,processing and preserving of tea and formulate scientific and resonable quality standard.
  • 药物制剂与药品质量控制
    CHEN Qinhua;LI Peng;ZHU Jun
    2011, 30(12): 1642-1643. https://doi.org/10.3870/yydb.2011.12.032
    ABSTRACTObjectiveTo develop a new method for assay of methylnnonylketone in Houttuynia cordata with gas chromatographymass spectrometry (GCMS).MethodsThe methylnnonylketone was analyzed quantitatively by using GCMS with the DB5MS quartz capillary chromatographic column (30 m×0.32 mm,0.25 μm)and pentadecane as internal standard in a selective ion monitoring way.The sample was extracted with nhexane.ResultsThere was a good linear relationship in the range of 0.5-5.0 mg•mL1 (r=0.993 8).The recovery was 98.25% and detection limit was 1.0 ng•mL1.ConclusionThe method is convenient,sensitive,accuracy and reliable.
  • 用药指南
  • 用药指南
    YE Lei;HU Lufeng;DU Xiaoxiang;LIN Guanyang
    2011, 30(12): 1651-1654. https://doi.org/10.3870/yydb.2011.12.036
    ABSTRACTObjectiveTo study the interaction of minocycline with human serum albumin (HSA)at different temperature and mechanisms underlying.MethodsThe quenching of fluorescence was determined by measuring fluorescence quenching spectra and synchronous fluorescence spectra of HSA interacted with minocycline.The type of binding force was estimated according to the thermodynamic equation.ResultsThe fluorescence of HSA interacted with minocycline was quenched regularly,with quenching constant as 3.29×104 L•mol1(T=299 K)and 3.53×104 L•mol1(T=310 K).The maximum emission peak of synchronous fluorescence spectra in HAS was redshifted by minocycline.ConclusionThe mechanism of the interaction of minocycline with HSA is a kind of dynamic quenching,which is mainly driven by a hydrophobic force.