中国科技论文统计源期刊 中文核心期刊  
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊  
日本科学技术振兴机构数据库(JST)
第七届湖北十大名刊提名奖  

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    药物研究
  • 药物研究
    LIU Xue-qin;YU Mei;ZHANG Yan;WEI Li-li
    Objective To investigate effects of polysaccharides from cultured mycelium of Cordyceps sinensis (Berk.) Sacc. (PCS) on insulin resistance in a fat-fed (FF) and streptozotocin (STZ)induced type 2 diabetic mice model. Methods Normal male Balb/c mice were i.p. injected with low-dose of STZ for consecutive 3 d and fed with high fat diet for 3 weeks simultaneously. PCS (200, 400 mg•kg-1) were administered orally to FF/STZ induced diabetic mice for 28 d. An oral glucose tolerance test (OGTT) followed the changes of fasting blood glucose, insulin resistance indexes(HOMA-IR), serum insulin and lipids were tested. Results T2DM-IR typical syptoms like abdominal obesity, glycemia and abnormal insulin level developed in FF/STZ induced diabetic mice. PCS reduced fasting blood glucose, total cholesterol, triglyceride and free fatty acid, and improved oral glucose tolerance dosedependently. The antidiabetic effects of PCS were also confirmed by lower serum insulin and insulin resistance indexes compared with untreated diabetic control mice. Conclusion Our findings suggest that PCS (400 mg•kg-1) ameliorates insulin resistance and prevents mice from developing hyperglycemia and hyperlipemia, probably by improving insulin sensitivity of target cells and increasing insulin receptor.
  • 药物研究
    CHEN Chen;WEI Xin;WANG Dao-wen
    Objective To investigate the effects of terfenadine derivatives with different structures on apoptosis in Tca-8113 cells. Methods Hoechst33258 stain and AO/EB stain were used to measure the apoptosis in Tca-8113 cells cultured with different compounds for 24 h. Results All four terfenadine derivatives (npropyl, allyl, bromideand vinyl) induced tumor cells apoptosis. The apoptosis rate measured by Hoechst33258 stain were (35.20±3.50)%, (59.69±4.70)%, (44.55±4.20)% and (56.32±5.10)%, respectively. The apoptosis rate measured by AO/EB stain were(55.14±9.70)%,(88.23±11.80)%, (72.47±9.30)% and (92.46±13.10)% , respectively. Conclusion All four different terfenadine derivatives promote apoptosis of Tca-8113 cells. The compounds with alkenyl structure have shown more significant proapoptosis effects, which may be related to an increase in the number of unsaturated bonds.
  • 药物研究
    JIANG Su-zhen;CHEN Yu-shan
    Objective To investigate the effect and mechanism of ethanol isolated dry ginger extract (EDGE) on intestinal movement in mouse and rabbit. Methods We observed the effect and mechanism of EDGE on normal, hyperfunctional and inhibitory state of intestinal motility through movement of Chinese ink along small intestine of mice and the isolated intestinal preparation of rabbits in vitro. Results EDGE obviously enhanced the normal,inhibitory state of intestinal motility of mice, but significantly inhibited the hyperfunction induced by Neo. EDGE obviously strengthened spontaneous constriction of normal or atropine induced rabbit ileum in vitro. But it exerted an inhibitory effect on the contraction induced by Ach in a dosedependent manner. It also reduced the contractive response of Ach in Ca2+free Tyrode’s solution while did not affect the contraction caused by CaCl2. Conclusion EDGE had the twoways regulation on intestinal motility, which could be related to Mreceptor.

  • 药物研究
    SHEN Yi-hui;LIU Jiang-kui;HE Jing-tang;YIN Ming;MA Lan-fang;LIU Xiao-li
    Objective To explore the effects of telmisartan on expression of adiponectin and PPAR-γ in rats with nonalcoholic steatohepatitis(NASH). Methods Thirtyfive male SD rats were randomized into normal model group(NC,n=10),NASH control group(FC,n=15) and telmisartan prevention group(FT,n=10). NC group was given with standard food, the other 2 groups were given with high fat diet for 16 weeks to induce NASH, and FT group was given with telmisartan (5 mg&#8226;kg-1&#8226;d-1) for 4 weeks by intragastric adminstration 12 weeks later. Fasting blood glucose and insulin were measured and insulin resistance value (HOMA-IR) was assessed by homeostasis mode assessment. Liver expression of adiponectin and PPAR-γmRNA were detected with RTPCR.The level of plasma adiponectin was detected with ELISA. Results The level of FBG, FINS and HOMAIR were signficantly higher in group FC than those in group NC (P<0.01), but those in group FT were signifcantly decreased than those in group FC(P<0.01). The level of adiponectin and adiponectin, PPAR-γmRNA were significantly decreased in group FC than those in group NC(P<0.01).The level of adiponectin and adiponectin, PPAR-γmRNA were higher in group FT than those in group FC(P<0.05).Conclusion Telmisartan has the protective and therapeutic effect for nonalcoholic steatohepatitis.
  • 药物研究
    CHEN Jun-hong;CHEN Cheng-shui

    Objective To evaluate the proper concentration of urokinase and time to dissolve cordal fiber texture by observing the dissolution of cordal fiber texture in different concentrations of urokinase solutions.Methods Cordal fiber texture obtained from thoracoscope was incubated with 0,500,1 000,2 000,4 000,6 000,8 000,10 000U&#8226;mL-1 urokinase, respectively. Then, the dissolution of cordal fiber texture was observed in 3 h. Results Using the same time quantum and different concentrations of urokinase,there was significant difference in dissolution between 0 U&#8226;mL-1 and the rest concentrations such as 500,1 000,2 000,4 000,6 000,8 000 and 10 000 U&#8226;mL-1 (P<0.05) when the time quantum was greater or equal to 1.5 h. However, there was no statistic significance among the concentions besides 0 U&#8226;mL-1 (P>0.05). Using the same concentration but different time quantums, there was no statistic significance between 0 U&#8226;mL-1 and 6 000 U&#8226;mL-1 urokinase.When the concentrtions reach to 500,1 000,2 000,4 000,6 000, 8 000, 10 000 U&#8226;mL-1 , there was no statistic significance among the time quantums a when the time quantum was greater or equal to 1.5 h, while within 1.5 h, the difference was significant.Conclusion The proper concentartion of urokinase solution dissolving cordal fiber texture is 500 U&#8226;mL-1, and the proper time is 1.5 h.

  • 泌尿生殖系统用药专栏
  • 泌尿生殖系统用药专栏
    WANG Hong-xiang;CHEN Bin;HU Kai;JIN Yan;HAN Yin-fa;YAO Xiao-dong;SHEN Jin-fang;WANG Yi-xin;HUANG Yi-ran
    Objective To investigate the efficacy of huangmai mixture treating oligoathenospermia due to spleen and kidney yang deficiency. Methods Sixtysix male infertility patients were randomized into experimental group and control group, given with huangmai mixture and compound xuanju capsule, respectively. All patients recruited went through a treating period of 24 weeks and came for followup visits at the 4th, 8th, 12th, 16th, 20th and 24th weekend, receiving clinical examinations and safety evaluation. Observation items included semen analysis, sperm morphology analysis, antisperm antibody, seminal plasma biochemical analysis and serum sex hormone. Results After treatment for 24 weeks, the total effective rates of experimental group and control group were 63.33% and 53.33%, respectively. The total effective rates between groups were not statistically significant(P>0.05). In total, 6 patients’ partners conceived during treating period (4 in experimental group and 2 in control group). Compared with those before treatment, the sperm density, sperm motility, a class sperm percentage, a+b class sperm percentage and sperm activity in the treated group and control group increased significantly (P<0.05). Fructose density and αglycosidase also increased, but the increasing degree showed no statistical significance. Serum sex hormone of two groups did not vary before and after treatment(P>0.05). Statistical significance was not observed in percentage increased between groups(P>0.01). Conclusion Huangmai mixture for treating oligoathenospermia due to spleen and kidney yang deficiency has the same efficacy and safety compared with compound xuanju capsule, with a relatively low cost, huangmai mixture is more suitable for clinical application.
  • 药物与临床
  • 药物与临床
    WU Chun-ling;LOU Han-jian;JIA Wei;CHEN Guo-zhong;TENG Yi-ming;ZHOU Xu-ling;SONG Qiu-xiang;ZHU Han-jun;WU Yi;HE Jian-xin
    Objective To study the clinical effect and prognosis of moxifloxacin in the treatment of early and no risk factory for MDR hospitalacquired pneumonia. Methods 60 cases of early and no risk factory for MDR hospitalacquired pneumonia were randomly divided into moxifloxacin (0.4 g ivgtt qd for 7 d) treatment group(30 cases)and ceftriaxone(2.0 g ivgtt qd for 7 d) treatment group(30 cases). The clinical indicators(CPIS,CRP),clinical Results ,clearance of pathogens,prognosis and security were observed. Results After treatment,the efficacy of the treatment group was 83.3%,the control was 66.7%. CPIS in the treatment group droped from(6.9±1.1)down to(3.0±1.9),CRP from(43.0±5.1)down to(17.6±3.9) mg&#8226;L-1; while CPIS in the control group declined from(7.1±1.4)to(4.6±1.7),CRP from(41.5±6.3)down to(30.8±4.4)mg&#8226;L-1. The pathogens clearance in the treatment and control groups was 81.0% and 63.2%, respectively. The incidence of later HAP and mortality in the treatment group was 6.7% and 3.3%, respectively; those in the control group was 16.7% and 10.0%, respectively. Conclusion Moxifloxacin can improve the clinical symptoms, shorten the course of treatment and improve prognosis for the early and no risk factory for MDR hospitalacquired pneumonia, which is efficient and security.

  • 药物与临床
    FENG Li;TIAN Yu-ke;WANG Peng;CHEN Ming-bing
    Objective To compare the effects of three general anesthesia Methods including intravenous compound anesthesia with midazolam, propofol or two drugs combined with inhalation of isoflurane on postoperative cognitive function of elderly patients. Methods 45 ASA Ⅰ-Ⅱ patients aged 60-80 years undergoing laparoscopic operation were randomly assigned on three groups: group A(n=15), midazolam was injected intravenously for anesthesia induction and midazolaminfusion was used in anesthesia maintenance; group B(n=15), propofol was given in anesthesia induction and propofolinfusion was used during surgery for anesthesia maintenance; group C(n=15), midazolam and propofol were given for anesthesia induction and during operation, and isoflurane was inhaled for anesthesia maintenance. The vital signs and AAI were monitored. The cognitive function tests were performed before surgery and at early recovery, medium recovery and late recovery after awakening. Results Early recovery: 5 min after awakening, the scores of patients in group A were significantly lower than values of preoperative, group B and group C (P<0.05). But 15 min after awakening, group A returned to preoperative level. And there was no significant difference between other two groups at every time points (5,15,30 min)( P>0.05). Medium recovery: 30 min after awakening, the scores decreased significantly in patients in three groups compared with preoperative values(P<0.05). Group A scored lowest(P<0.05) and group B, group C by turns(P<0.05); 1 h after awakening, the scores of group A and group B increased significantly and there was significantly difference between them(P<0.05), but they were all lower than preoperative values(P<0.05), at this time the scores of patients in group C returned to preoperative level. And so did group B at 2 h, yet the cognitive function level of group A was still lower than preoperative values(P<0.05). Late recovery: 4 h after awakening, the scores of patients in group A and group B were both lower than preoperative values(P<0.05). Group A scored lower(P<0.05) and there was no significant difference between the scores of group C and preoperative values(P>0.05). 24 h after awakening, the scores of group B returned to preoperative level(P>0.05), but the cognitive function of group A had not recovered(P<0.05). 72 h after awakening, the cognitive function level of group A descent significantly(P<0.05), and there was no significantly difference in other two groups compared with preoperative values(P>0.05).Conclusion The three Methods of general anesthesia have some influence on the cognitive function of elderly patients three days after surgery. The total effect of midazolambased anesthesia is most serious and lasts longer, and the influence of propofolbased anesthesia is much slighter. Yet the cognitive function of patients treated with midazolam and propofol intravenous combined with inhalation of isoflurane anesthesia is recovered the quickest.

  • 药物与临床
    YU Ye-an;XIA Yuan-yu;YIN Qing-qiao;LI Xiang-you
    Objective To study the effects of levocarnitine by intravenous injection on microinflammatory state and correlation inflammatory factor in maintenance hemodialysis patients. Methods Sixtyeight maintenance hemodialysis(>12 months) patients in our dialysis center were enrolled, and were randomly divided into treatment group and control group.Patients in the treatment group were injected with levocarnitine (1.0 g once) after every dialysis for 3 months,while patients in the control group only accepted routine hemodialysis therapy.Blood biochemical indicators, serum hs-CRP, IL-6, IL-8, TNF-a,Lp(a) and Kt/V value were measured and compared at the experiment onset, 1 month and 3 months later.Results The hs-CRP, IL-6, IL-8, TNF-α and Lp(a) level were obviously decreased in the treatment group after 1 month,and there were significantly difference between two groups(P<0.05).All above markers in the treated group were descended further after 3 months, and the difference between two groups was more significant(P<0.01). Conclusion Levocarnitine can reduce serum level of hs-CRP,IL-6,IL-8, TNF-α and Lp(a) and improve the microinflammatory state in maintenance hemodialysis patients.
  • 药物制剂与药品质量控制
  • 药物制剂与药品质量控制
    XIN Hai-liang;SHENG Jia-yu;GE Ling-xian;YUE Xiao-qiang;SU Yong-hua;LI Min;LING Chang-quan
    Objective To optimize the process conditions and parameters for extracting and purifying technology of ginseng leaves extract. Methods Using orthogonal test to determine extracting conditions of ginseng leaves extract, static adsorpting test to select the resin. Meanwhile, the dynamic adsorption was used to investigate the effect of sample concentration, flow rate, adsorption capacity and other factors on the dynamic adsorption properties of resin. Contents of Ginsenoside Re was determined by HPLC and yield rate was calculated by weight analysis. Results Extraction process: rough ginseng leaves were refluxed with 50% alcohol twice (12 times of solution and 2 h each time). Purification process: D101 macroporous resin (4 cm×16 cm, column volume 140 mL, dead volume 30 mL) was selected to study the dynamic adsorption of resin, the proper concentration was 0.4 g&#8226;mL-1, the largest sample added was 160 mL, adsorption rate was 1.5 BV&#8226;h-1. The absorbed sample was eluted with 6 BV water, 40% ethanol, 95% ethanol successively, and the 40% ethanol eluted part was combined and evaporated in vacum to obtain the gingsen leaves extract. Conclusion The technolgy is convenient and feasible for extracing and purifying ginseng leaves extract.
  • 药物制剂与药品质量控制
    ZHOU Xue-gang;ZHANG Li-ping;WANG Yan-fang;WANG Zong-xi;WANG Qi-feng;LIAO Zi-rong
    Objective To study effects of temperature and time of the peony root cooking on content of paeoniflorin; and determine it from cooked, uncooked, underground sections of peony. Methods The HPLC method was used with a LichroCART RP-C18(4 mm×250 mm,5 μm)column at 30 ℃. The mobile phase composed of HCN-0.1% phosphoric acid(V:V=14:86), with a flow rate at 1.0 mL&#8226;min-1. The detection wavelength was 230 nm. Results Cooking temperature and time largely affected the changes of paeoniflorin; the contents of paeoniflorin after being cooked (85 ℃, 15 min) in different species of peony were higher than precooked ones. The content of peoniflorin in different parts of peony root was revealed as: rhizoma>fibrous root>axial root. Conclusion There should be a clear indicator for cooked Peony root. and a systematic and comprehensive study on it. The one alone indicator is not enough for research. Whether or not peeled off for the root of peony should be explicited.

  • 药物制剂与药品质量控制
    ZHANG Xiao-yin;SHI Da-wei;ZHANG Chun-hong
    Objective To investigate the application of sulfobutyl-ether-β-cyclodextrin (SBE-β-CD) in eye drops of balofloxacin preparation. Methods Prepared the eye drops of balofloxacin with and without SBE-β-CD. Comparatively investigated their drug content with UV spectrometry, related substances with HPLC, stability under light and heat, as well as the eye irritations. Results SBE-β-CD significantly reduced eye irristations of the eye drops with drug. SBE-β-CD also significant improved the stability of eye drops of balofloxacin under light. Conclusion The inclusion by SBE-β-CD effectively prevents degradation of balofloxacin under light.


  • 药物制剂与药品质量控制
    WANG Wei;LIU Zu-xiong;YANG Xiao-song
    Objective To study the method of preparation and quality control of loxoprofen sodium plastic. Methods Loxoprofen sodium plastic was prepared with chitosan as plastic material and high performance liquid chromatography (HPLC) was used to determine the content of loxoprofen sodium. Results The linear range of loxoprofen sodium was 10-90 μg&#8226;mL-1,while the average recovery rate was 100.22%, and RSD was 0.44% (n=9).Conclusion The preparation technology for loxoprofen sodium plastic is reasonable, and quality control is feasible, both of which are consistent with relevant regulations of plastic preparation of China pharmacopoeia(2005 edition).

  • 用药指南
  • 用药指南
    HUA Jun-yan;LIU Bing-jin;TIAN Wei-qiang;WEI Yi-juan
    Objective To investigate Pseudomonas aeruginosa infection distribution and drug resistance of different types of antibacterial agents in our hospital from January to December on 2009, to guide the clinical rational use of medicines. Methods 340 cases of Pseudomonas aeruginosa infection in our hospital were collected, the distribution of clinical infection, bacterial resistance and retrospectively clinical medication were analyzed, respectively. Results The most Pseudomonas aeruginosa were isolated from sputum samples,with the positive rate as 80.3%;the highest sensitive agent to Pseudomonas aeruginosa was polymyxin(account for 97.1%),followed by amikacin(91.8%),obramycin(83.2%);the highest resistance was SMZTMP(account for 97.4%),followed by klinomycin(92.6%),mezlocillin(70.9%). The metalloenzyme masculine strain was only sensitive to polymyxin B, while resistant to other antibiotics. Conclusion Pseudomonas aeruginosa infection in hospital is in a tough situation. The drug sensitivity Results suggest that doctors should choose antibiotics reasonable,in order to reduce the emergence of drugresistant strains.

  • 用药指南
    DUAN Xue-yun;FENG Han-ge;CHEN Shu-he;WU Bing-bing;FAN Heng
    Objective To investigate the compatible stability of cefodizime sodium and omeprazole sodium for injection without light.Methods The content of the mixture at 4 ℃, 25 ℃ and 37 ℃ for 8 h were determined by HPLC method, and its external appearance and pH value were observed.Results There were no significant changes in appearance and pH value of the mixture for injection at 4 ℃ for 8 h, 25 ℃ for 6 h and 37 ℃for 2 h, respectively, the variation was less than 5%.Conclusion The mixture of cefodizime sodium and omeprazole sodium for injection can be used after storing at 4 ℃ for 8 h, 25 ℃ for 6 h and 37℃ for 2 h.