Archive

• 01 August 2011 Volume 30 Issue 08

    

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  药物研究
  心血管系统用药专栏
  药物与临床
  药物制剂与药品质量控制
  

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药物研究
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  药物研究

  Effect of Reinforcing Kidney Replenishing Qi and Promoting Blood Circulation Recipe onthe Expression of IGFⅠ and IGFBP3 in Brain of the Fetal Rat with FGR

  WANG Qi;XIONG Liping;TU Qingnian

  2011, 30(08): 975-978.

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  ObjectiveTo investigate the effect and mechanism of reinforcing kidney, replenishing qi and promoting blood circulation recipe on the protein expression of IGFⅠ and IGFBP3 in the brains of rat fetal growth restriction (FGR).MethodsThe FGR model was created by passive smoking in SD rats .Thirtytwo pregnant SD rats were randomly divided into four groups: control group, FGR group, FGR rats treated with reinforcing kidney, replenishing qi and promoting blood circulation recipe (Chinese medicine group), FGR rats treated with Larginine (Larginine group).The protein expression of IGFⅠ and IGFBP3 in the fetal brain was analyzed by immunohistochemistry.ResultsCompared with the FGR model controls ［（0.148 3±0.015 6） and （0.168 9±0.021 4）］, the expression of IGFⅠ and IGFBP3 in fetal brains of Chinese medicine treated group ［（0.191 3±0.017 8） and （0.187 5±0.023 3）］ increased significantly(P＜0.05); in addition, the expression of IGFⅠ was higher than that of Larginine treated group ［（0.193 3±0.020 3）］ as well (P＜0.05).The difference between the Chinese medicine treated group and the model control one was insignificant (P＞0.05).ConclusionThe recipe of reinforcing kidney, replenishing qi and promoting blood circulation recipe promotes fetal growth in uterus by regulating the expression of IGFⅠ and IGFBP3.
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  药物研究

  Extraction of Liposoluble Components in Salvia Bunge f.alba by Supercritical Fluid Carbon Dioxide and Chemical Analysis by GSMS

  WANG Haiyan;LI Yuqin;WANG Guangxu

  2011, 30(08): 978-981.

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  ObjectiveTo isolate the liposoluble components in Salvia Bunge f.alba by supercritical fluid carbon dioxide and take a chemical analysis by gas chromatography mass spectrometry(GSMS).MethodsL9(34) orthogonal test was used to optimize the supercritical fluid extraction (SFE) of liposoluble components in Salvia Bunge f.alba based on extracting temperature, duration, pressure and concentration of entrainer and the chemical composition of which was analyzed by GSMS.ResultsThe optimal process was making the extraction for 1.5 h at 45 ℃, under the pressure of 25 MPa, and with 90% ethanol as a modifier. 22 compounds were identified by GCMS, in which the main constituents were ferruginol (5.54%), tanshinon IIA (12.54%), cryptotanshinone (8.39%), and isocryptotanshinone (11.92%).ConclusionThe SFE technique was successfully applied to the extration of the liposoluble in Salvia Bunge f.alba.The extraction could be a kind of important materials coating a lot of tanshinons and unsaturated fatty acids.The result offered a theory guidance to further empolder and utilize Salvia Bunge f.alba .
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  药物研究

  Protective Effects of Ferulic Acid on Human Keratinocyte

  WANG Gang;ZHANG Xiuhua;YANG Jinxia;CHANG Mingquan;YANG Guangyi;YE Fang;DUAN Dejian

  2011, 30(08): 982-984.

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  ObjectiveTo investigate the protective effects and mechanism of ferulic acid on human keratinocytes(HKC). MethodsThe cell model of oxidative stress was established in HKC treated with CoCl2.The cell viability post cultured with ferulic acid was detected by MTT method, the secretion of cytokines TNFα and IL8 in the supernatant was measured by ELISA.ResultsThe surviving rate of HKC cell with normal group was 100.00% and the contents of TNFα and IL8 was 11.37，98.30 pg•mL1 respectively.The surviving rate of HKC cell respectively was 96.15%，67.92% in group treated with 0.03 and 0.3 mg•mL1Ferulic acid，which were significantly higher than those in the model group (46.20%，P＜0.01，P＜0.05),and the effect was in a dosedependent manner.The contents of TNFα(respectively were 12.95 ,17.46 pg•mL1)were significantly lower than those in the model group (29.54 pg•mL1,P＜0.01，P＜0.05), The contents of IL8(respectively were 102.76，128.96 pg•mL1)were significantly lower than those in the model group(162.33 pg•mL1,P＜0.01,P＜0.05 ).However, There were no significant effects on the surviving rate of HKC cell and the contents of TNFα and IL8 in group treated with 0.003 mg•mL1Ferulic acid.ConclusionFerulic acid may prevent HKC against oxidative injury by inhibiting secretion of TNFα and IL8.
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  药物研究

  Morphological Study on Urethral Sphincter Muscle Injury Treated by InsulinLike Growth Factor-I

  RAO Qun;DU Guanghui;CAI Dan;ZHUANG Quanyuan;CHEN Zhong;YANG Weimin;YE Zhangqun;CHEN Yuan

  2011, 30(08): 984-987.

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  ObjectiveTo study the microstructural and morphological changes following urethral sphincter muscle injury of female rats and evaluate the therapeutic effect of periurethral injection of insulin like growth factorI（IGFI）.MethodsModel of urethral sphincter muscle injury was made in female virgin SD rats(n=40）by intravaginal balloon inflation.The treatment group(n=20) was taken periurethral injection of 1.0 μg human IGFI in the middle urethral muscle, the control group was given with 10 μL saline injection,and four normal rats were used as a blank control without any surgery and treatment.Four rats in each group were killed at 2,4,6,8,14 day, respectively.The one third of middle urethra specimens was harvested for histological study with Masson’s trichrome staining to quantify the composition of striated muscle, smooth muscle and dense connective tissue.ResultsThe break, atrophy，structure distortion and collagen fibers hyperplasia were obviously found in the middle striated muscle layer.Compared with the saline control group, the ratio of crosssectional area of the striated muscle to smooth muscle increased significantly on day 8 and day 14(P＜0.05) in the IGFI treatment group, which showed more muscle fiber hyperplasia and intact morphological structure.ConclusionLocal injection of IGFI stimulates the repairing of injured urethral spincter muscle, which may play a role in treatment of stress urinary incontinence induced by urethral sphincter muscle dysfunction.
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  药物研究

  Contents Study of Two Alkloids in Wutou Decoction with Different Proportion

  LI Ling;LIU Diangao;ZHANG Yiwen;ZHAO Chunjie

  2011, 30(08): 987-990.

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  ObjectiveTo investigate the contents of mesaconiatine and hypaconitine in wutou decoction with different proportion.MethodsAconiti Radix Cocta was combined with other herbs in different ratio, and the content of mesaconiatine and hypaconitine in which was investigated by HPLC.ResultsFor mesaconiatine and hypaconitine, the linear regression equation were set as Y=4.253X+30.98（r=0.999 4） and Y= 3.713X+96.02（r=0.999 3）, with the linear range of 4.95－79.20 μg•L1 and 12.18－194.80 μg•L1, respectively.It presented a great influence on the contents of mesaconiatine and hypaconitine by the prepared Aconiti Radix being combined with other herbs at different ratio.The best wutou decoction composed of 6 g prepared Radix Aconiti, 5 g Ephedra, 13 g Astragali Radix, 7 g Paeoniae Radix Alba, 13 g prepared Radix Glycyrrhizae, which leading to the optimum contents of mesaconiatine and hypaconitine.The linear relation of mesaconiatine and hypaconitine was good within their linear range.ConclusionThe investigation on the combinations of wutou decoction based on the content change of mesaconiatine and hypaconitine can ensure the efficacy and reduce the toxicity of wutou decoction.
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  药物研究

  Effect of Cepharanthine on Pharmacokinetics of Fexofenadine in Rats

  HU Yuqin;JIANG Xuehua;YU Zhiying;TAO Yong;HUANG Meilan

  2011, 30(08): 991-993.

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  ObjectiveTo study the effect of cepharanthine on pharmacokinetics of fexofenadine in rats.Methods10 rats were randomly divided into two groups, which were treated with fexofenadine as group A and fexofenadine plus cepharanthine as group B.The blood samples were collected at different time points from two groups.The serum concentration of fexofenadine sodium was determined by HPLC and the pharmacokinetic parameters were calculated with 3P97 program and analyzed.ResultsThe Cmax and AUC0t were significantly elevated in the drug combination group compared with those in the group treated with fexofenadine alone.The AUC0t in group A and group B was (5.379±1.570) and (8.088±1.760) mg•h•L1(P＜0.01); the Cmax was (1.225±0.770) and (1.417±0.440) μg•mL1 (P＜0.05)，the AUC0t and Cmax were increased 7.09%(P＜0.05) and 15.67% (P＜0.05)，respectively; however，no significant differences were noted with regard to Ka, MRT and t1/2.ConclusionThe marked increase of Cmax and AUC0t of fexofenadine when fexofenadine and cepharanthine were coadministered，indicating that the oral bioavailabilities of fexofenadine were significantly increased.
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  药物研究

  Study on the Liver Protection，Jaundice Receding and Liver Enzyme Decreasing Effect of Lingwu Granula

  XU Xueyu;LIU Jun;YU Shan;LIU Qingchuan;CHENG Lijuan;HUANG Zhengming

  2011, 30(08): 994-997.

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  ObjectiveTo study the liver protective effects and mechanism of lingwu granula on liver injury in a mice model of jaundice induced by αnaphthylisothiocyanate(ANIT).MethodsMice were randomly divided into six groups: normal group， ANIT model group， lingwu granula treatment groups (2.5，5，10 g•kg-1) and yinzhihuang granula group (2.34 g•kg-1).After intragastric administration of certain medicines in each group for 7 days except normal and ANIT model groups which were treated with NS，mice were i.g.to induce jaundicetype liver injury model (105 mg•kg-1) .The blood and liver tissue were collected to detect the serum contents of alanine aminotransferase（ALT）， aspartate aminotransferase（AST），total bilirubin（TBiL） and the activities of malondialdehyde（MDA）， superoxide dismutase（SOD），glutathione（GSH） in liver.The hepatic histopathology was detected.ResultsThe contents of TBiL，ALT，AST of ANIT model group were increased obviously compared with those in the normal group.However，lingwu granula significantly decreased the content of ALT， AST， TBiL in serum in a good dosedepend manner compared with ANIT model group (P＜0.01).In addition，lingwu granula at middle and high dose significantly lowered the activities of MDA (P＜0.01) and reinforced the activities of SOD (P＜0.01) and GSH (P＜0.05 and P＜0.01) in liver as well.ConclusionLingwu granula protects liver injury induced by ANIT， brings down liver enzyme and recedes jaundice, which may be related to its antioxidation effect.
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  药物研究

  Percutaneous Absorption of the Compound Lidocaine Cream in Vitro

  SHU Chengren;GE Miaomiao;HUANG Lu;WU Haiyan;LI Weiyong

  2011, 30(08): 997-999.

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  ObjectiveTo compare the percutaneous absorbency of the compound lidocaine cream between the lab made and commercially available one during different periods of storage.MethodsThe permeation of the cream was studied on rat skin with Franz diffusing cell.Lidocaine and prilocaine was quantified by HPLC.The percutaneous absorbency in vitro was evaluated by transdermal absorption accumulation of lidocaine and prilocaine.ResultsThere were no significant difference in the transdermal absorption accumulation between the test cream and the reference one (P＞0.05). And no significant difference was found in the transdermal absorption and drug release whenever within the expiry date (P＞0.05).ConclusionThe percutaneous absorbency of the test cream is consistent with the reference cream, and which is stable during storing within the period of validity.
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  药物研究

  Concentration Determination of Ziprasidone and Aripiprazole in Human Plasma by RP-HPLC

  LIU Weizhong;CHEN Qingxia;MO Yuquan;WEN Yuguan;WANG Guangfa

  2011, 30(08): 1000-1003.

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  ObjectiveTo establish a method for determining the concentration of ziprasidone and aripiprazole in human plasma by RPHPLC.MethodsThe plasma level of drugs were analyzed by a reverse phase HPLC system with a C18 column(150 mm×4.6 mm， 5 μm), 0.03 mol•L1 ammonioummethanol(14：86) as mobile phase, flowing at 0.8 mL•min1, and the detection wavelength at 254 nm.Ethyl acetate and dichloromethane(80：20) were used as extracting solvent.ResultsThe relation of peak area and concentration showed linear in the range of 20.0－640.0 ng•mL1 for ziprasidone，25.0－1 000.0 ng•mL1 for aripiprazole，respectively.The average recoveries of ziprasidone and aripiprazole at high，middle and low concentrations were 96.95%，97.71%， 100.21% and 97.87%， 98.36%， 101.82%， respectively; the extraction recovery was 71.42%， 74.33%， 78.26% and 72.67%， 76.51%， 79.32%， respectively.The intraday and interday variations(RSD) were less than 9% (n=5).The minimum detectable concentration was 10.0 ng•mL1.The regression equation of ziprasidone was Y=61.717X＋2.16, r = 0.998 9（n=7）and aripiprazole was Y=87.946X＋1.37, r= 0.999 1（n=7）, respectively.ConclusionThe method is sensitive，accurate，simple and quick，which can be used for clinical drug monitoring and pharmacokinetic studies of ziprasidone and aripiprazole.
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  药物研究

  Pharmacokinetics of Complex Prescription of Roxithromycin Tablets by Multiple Dose of Administration in Healthy Volunteers

  REN Xiuhua;SHEN Lingling;XIANG Daochun;LIU Yu;CHEN Qian;ZHANG Donglin;FANG Shuxian;LIU Dong;DU Guang

  2011, 30(08): 1003-1006.

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  ObjectiveTo study the pharmacokinetics of complex prescription of roxithromycin tablets(I) by multiple dose of administration in healthy volunteers.MethodsThe total of 24 healthy volunteers were randomly divided into two groups with twelve each, and given with multiple dose of I or roxithromycin tablets plus ambroxol hydrochloride tablets(II)， respectively.The concentration of roxithromycin or ambroxol in plasma was determined by LCMS/MS separately.The pharmacokinetic parameters were calculated with DAS 2.1 practical program and evaluated by ttest.ResultsThe Cmax，t1/2， AUC(0→t) and AUCs of roxithromycin and ambroxol of I were（ 6 261.67±2 111.12 ）and 51.42±19.83 ng•mL1，（14.70±8.91）and （10.19±1.94）h，（96 055.25±38 428.39） and （679.86±183.90 ）ng•mL1•h，（47 767.15±16 097.92） and（372.53±119.18） ng•mL1•h，respectively.ConclusionCompared with II，the pharmacokinetic parameters of roxithromycin and ambroxol of I showed no significant difference.

心血管系统用药专栏
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  心血管系统用药专栏

  The Plamsa Levels of Nerve Growth Factor and Interference by Captopril in Children with Congestive Heart Failure

  JIAO Rong;ZHU Xiaoyan

  2011, 30(08): 1013-1015.

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  ObjectiveTo study the levels of plasma nerve growth factor（NGF) in children with congestive heart failure（CHF) and its changes after treatment of captopril.Methods25 children with CHF were randomly divided into 2 groups: ordinary regimen group（n=12) and captopril treatment one （n=13).The plasma concentration of norepinephrin（NE) and nerve growth factor were measured by high performance liquid chromatography and a twosite enzymelinked immunosorbent assay（ELISA), respectively.20 healthy children were selected as controls.Digoxin, diuretics and the vasodilatation agent were given as conventional treatment.Catto Pulley was added besides the conventional treatment, to the young children 1～2 mg•kg1•d1, bid for 3 months.Results①Plasma concentrations of NE were markedly increased and NGF were decreased significantly in CHF group, compared with those in the normal control group; There was correlation between the levels of NE，NGF and cardiac function, and a negative correlation between NGF and NE（r=－0.771,P＜0.01）.②3 months after therapy, the levels of NE in ordinary regimen group were significantly decreased（P＜0.01）, NGF were increased （P＜0.05), there was no significant difference in ejection fraction（EF）levels compared with those before treatment （P＞0.05); In captopril regimen group, the levels of NE were significantly decreased（P＜0.01）, NGF and EF were significantly increased （P＜0.01); There were significant differences between two groups in the levels of NE，NGF and EF after treatment （P＜0.01).③The cardiac function was improved in both groups, especially in the captipril treated group after 3 months（P＜0.01).ConclusionNGF are involved in the process of CHF, captopril can prevent and reverse the neurohumoral disorders in CHF and improve the cardiac function of children with CHF.
药物与临床
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  药物与临床

  Effect of Salmeterol/fluticasone on Serum Levels of Interleukin21 and Total IgE in Asthmatic Patients

  LI Kaiyan;XIONG Shengdao;ZHU Jing;CAO Yong;XIONG Weining

  2011, 30(08): 1027-1029.

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  ObjectiveTo investigate the combined effect of salmeterol/fluticasone(SF) therapy on serum levels of interleukin21 (IL21) and total IgE in asthmatics， and to explore the relationship between them in patients with allergic asthma.MethodsA total of 95 blood samples were collected and divided into three groups: control group (n＝20) ，allergic asthma group (n＝45) and treatment group (n＝30).Patients belonging to the treatment group were treated with a combination of 50 μg salmeterol and 250 μg fluticasone twice daily for three months， and it was found that their asthma symptoms were alleviated.The levels of IL21 and total IgE in blood were detected by enzymelinked immunosorbent assay (ELISA).ResultsThe serum levels of IL21 in control group， allergic asthma group and treatment group were 17.19， 21.99 and 17.18 pg•mL-1， respectively.The levels of IL21 significantly differ from each other between the control group and allergic asthma group (P＜0.05).Negative relationship was found between the levels of IL21 and total serum IgE in allergic asthma group (r=－0.32， P＜0.05).In addition， IL21 level were significantly reduced by SF in treatment group compared to allergic asthma group (P＜0.05)， while SF did not significantly change total IgE levels (P＜0.05).ConclusionOur results suggested that combined effect of salmeterol/fluticasone inhalation can reduce levels of IL21 in asthmatic patients， and it might be one of the important mechanisms for the treatment of asthma.

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  Effects of 6% Hydroxyethyl Starch 130/0.4 on Renal Function of Elderly Patients underEsophagus Carcinoma Surgery

  SU Peng;HE Xiaofeng;YAO Ying

  2011, 30(08): 1030-1033.

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  ObjectiveTo explore the influence of 6% hydroxyethyl starch 130/0.4 on renal function of elderly patients undergoing esophageal cancer surgery.MethodsThe 54 elderly patients received esophageal cancer surgery were enrolled and randomly divided into two groups and undertook acute hypervolemic hemodilution（AHH）.6% hydroxyethyl　starch 130/0.4 sodium chloride injection and sodium lactate ringer’s injection were given to the test and control groups, respectively.The heart rate（HR）,saturation of arterial blood oxygen（SaO2）,mean arterial pressure(MAP), central venous pressure(CVP),creatinine（Cr）,blood urea nitrogen（BUN）,uric acid（UA）and Cysteine protease inhibitors C（CystatinC） were monitored at before AHH(t1)、post AHH(t2)、2 hours after AHH(t3)、4 hours after AHH(t4) and 24 hours after AHH(t5).ResultsThere was no significant difference occured for MAP and HR between the two groups.CVP in both groups was increased significantly at t2－t4 compared with that at t1 and t5.CVP in the test group was higher than that in the control group at t2, t3 and t4.No remarkable changes for Cr，BUN and UA were found whatever time points, intergroups or intragroups.Serum concentrations of were normal during the study.CysC at t5 in both groups were higher than that at t1－t4, and there was no significant difference between two groups.ConclusionAHH with 6% hydroxyethyl starch 130/0.4 has no obvious harmful effect on renal function of the elderly patients who undertaken surgical treatment for esophageal cancer.
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  药物与临床

  Efficacy and Safety of Specific Immunotherapy Plus Corticosteroid for Patients with Dust Miteinduced Allergic Asthma

  ZHU Rongfei;LIU Guanghui;HUANG Nan;LI Wenjing;LIU Huilong

  2011, 30(08): 1033-1037.

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  ObjectiveTo evaluate the clinical efficacy and safety of specific immunotherapy（SIT）plus inhalant corticosteroid（ICS） for patients with dust mite-induced allergic asthma.Methods92 eligible patients were divided into two groups，58 in the test group and 34 in the control one.The patients in test were treated with injection dermatophagoidei farini(dermatophagoides pteronyssinus) plus budesonide inhalation for 1a , the former of which started gradually from 1st grade（50 TU•mL1）0.1 mL to 3rd grade（5 000 TU•mL1）0.8 mL for maintaining treatment, and the average initial dosage of ICS was (430±200) μg•d1.The patients in control were treated with budesonide in halation for 1 a, the average initial dosage of ICS was (420±205) μg•d1.The ACT survey and lung function test were performed for all tested patients every 3 months.The dosage of ICS were reduced 50% if the asthma were well controlled.The quality of life was assessed in all patients by AQLQ before and after the treatment.Adverse drug reactions were recorded during the entire study process.ResultsThe asthma, lung function, AQLQ score and the daily ICS usage were significantly improved after treatment in both groups(P＜0.05).The asthma control rate in the test group was 93.1%, which was higher than that in the control group(70.6%)(P＜0.05).The average ICS usage in the test was (260±80) μg•d1,which was lower than that in the control ［(350±160) μg•d1］ (P＜0.05) .There were no significant differences appeared for the lung function and AQLQ score between the two groups after treatment（P＞0.05）.The main adverse reactions of SIT were nodule, redness and swelling in the injection site and no severe side effects occured in the whole study.ConclusionThe efficacy of SIT combined ICS is better than single ICS in the treatment of allergic asthma and the combined therapy has a good safety profile.
药物制剂与药品质量控制
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  药物制剂与药品质量控制

  Preparation and Quality Control of Amlexanox Troches

  FU Xudong;LIU Zuxiong;LI Ruihua

  2011, 30(08): 1066-1069.

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  ObjectiveTo prepare amlexanox troches and establish a method for its quality control.MethodsThe contents of amlexanox were determined by HPLC.The LichrospherC18(250 mm×4.6 mm,5 μm) was taken as chromatographic column, and methanol0.01 moL•L1ammonium phosphate monobasic (70：30) was used as the mobile phase.And the flow rate was set at 1.0 mL•min1, detection wave length as 350 nm, column temperature at 25 ℃, and the sample was loaded with 20 μL.ResultsThe linear range of amlexanox was 1～24 μg•mL1(r2=0.999 8)，the average recovery rate was 100.07%，and RSD was 1.46%（n=9）.The tests of uniformity, dissolubility and fragility for 3 batches of samples were accorded with the quality criteria.The amlexanox troches were made presented with good taste，smooth sruface，good shape, uniform color，appropriate rigidity，and convenient in use.ConclusionThe preparation process for amlexanox troches is reliable，easy in measurement，accurate，which fits making the clinical preparations.
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  药物制剂与药品质量控制

  Preparation and Content Determination of Quercetin Solid Lipid Nanoparticles

  CHANG Mingquan;SUN Yune;JIANG Anrong;WANG Gang

  2011, 30(08): 1069-1071.

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  ObjectiveTo establish a preparatian process and content determination method for quercetin solid lipid nanoparticles(QUESLNs).MethodsThe oil phase for preparing QUESLNs was mainly made of Glyceryl behenate (ATO)，soy lecithin and cholesterol, and the aqueous phase was made of poloxamer, PEGDPSE and Tween 80.The temperature for emulsion was set at 75 ℃ , for solification from ice to water was at 2 ℃，and the agitator for emulsion was 600 r•min1.The quercetin was determinated by a highvelocity centrifugation and HPLC.The Diamonsil C18 (250 mm×4.6 mm,5μm) was used, methanolacetic acid (55：45) was taken as mobile phase at 1.0 mL•min1, column temperature was 30 ℃ and the sample was loaded with 20 μL.ResultsQUESLNs were stable and presented as linear relation at the range of 20.0～200.0 μg•mL1，A=1.343 5C－1.490 4(r=0.999 6). The average recovery was 97.7% and RSD was 1.09%(n =6).ConclusionPeparation process for QUELCL is stable and content determination is simple，reliable，accuracy，and feasibility.
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  药物制剂与药品质量控制

  Preparation and in vitro Release of the Newtype Microspheres of Silver Sulfadiazine

  YAN Jingjing;LANG Yiyong;WANG Qiang;ZHU Xiaohong;LI Sanming

  2011, 30(08): 1072-1075.

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  ObjectiveTo prepare the chitosanalginate sodium microspheres loaded with silver sulfadiazine and study their release characteristics.MethodsThe emulsion crosslinking technique was applied to produce the microspheres, and the diameter, morphology, and in vitro release behavior of which were studied.ResultsThe appearance of microspheres was round and intact, the average diameter of which was 38.2 μm, and the 5 h in vitro drug release was completely.ConclusionThe process of preparing microspheres is simple and easy, and the drug release is quick, which deserving further research as the wound protective materials.
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  药物制剂与药品质量控制

  Studies on Fingerfrint of Herba Glechomae by HPLC

  LIAO Yaling;AN Wei;HU Guangxu

  2011, 30(08): 1075-1080.

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  ObjectiveTo establish a fingerprint analysis method for Herba Glechomae by HPLC.MethodsThe detection was carried out with methanol and water as a mobile phase at a flow rate of 1.0 mL•min1, and with the detection wavelength as 0→60 min 330 nm，60→90 min 280 nm, at 40 ℃.ResultsFingerprint analysis for Herba Glechomae was established by HPLC.13 copossessing peaks were identified and the similarity of 10 batches Herba Glechomae was between 0.905－0.978.ConclusionThe method is stable, reliable and provides an effective way for quality control of Herba Glechomae.
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  药物制剂与药品质量控制

  Content Determination of 11 Metal Elements in Decoction of Rheum and Moutan by Flame Atomic Absorption Spectrometry

  LIU Li;CAO Yan;JIANG Jun;ZHAO Chunjie

  2011, 30(08): 1080-1083.

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  ObjectiveDetermine the content of 11 metal elements (Ca，Cu，Fe，K，Mg，Ni，Cr，Mo，Zn，Cd，Na) in decoction of rheum and moutan.MethodsThe airacetylene flame atomic absorption spectrometry was used to determine the elements in decoction of rheum and moutan and the accuracy and precision of which were studied.The samples were digested with HNO3HClO4 (4：1) at 140 ℃ under normal pressure.ResultsThe RSD of 11 metal elements and recoveries were 0.68%～2.00% and 96.8%～99.7%， respectively.ConclusionThe method is simple，rapid and appropriate for content determining of the 11 metal elements，which providing an effective basis for evaluating clinical efficacy of Rheum and Moutan decoction.